Restriction map for
pFN21AE5785
(Circular) (Six-base) MAPSORT of: pFN21AE5785.seq Check: 6963 from: 1 to: 6994
pFN21AE5785 6994 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2371 3682 2371
Size: 1311 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4804 278
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4337 1937
Size: 2400 4594
Acc65I G'GTAC_C
Cuts at: 3891 4315 3891
Size: 424 6570
AclI AA'CG_TT
Cuts at: 1563 4293 5121 5494 1563
Size: 2730 828 373 3063
Fragments arranged by size:
3063 2730 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 2956 3862 5057 6441 6709 1632
Size: 1298 26 906 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1298 1195 906 268 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6797
AflIII A'CryG_T
Cuts at: 1203 5893 6870 1203
Size: 4690 977 1327
Fragments arranged by size:
4690 1327 977
AgeI A'CCGG_T
Cuts at: 5825 5825
Size: 6994
AhdI GACnn_n'nnGTC
Cuts at: 5723 5723
Size: 6994
AleI CACnn'nnGTG
Cuts at: 1166 3233 3447 1166
Size: 2067 214 4713
Fragments arranged by size:
4713 2067 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3839 1184
Size: 2655 4339
AlwNI CAG_nnn'CTG
Cuts at: 2239 3688 4091 4737 6309 2239
Size: 1449 403 646 1572 2924
Fragments arranged by size:
2924 1572 1449 646 403
ApaI G_GGCC'C
Cuts at: 3970 3970
Size: 6994
ApaLI G'TGCA_C
Cuts at: 5051 6207 5051
Size: 1156 5838
ApoI r'AATT_y
Cuts at: 1493 3127 4303 4529 4681 6790 1493
Size: 1634 1176 226 152 2109 1697
Fragments arranged by size:
2109 1697 1634 1176 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5548 160
Size: 872 4516 1606
Fragments arranged by size:
4516 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
AvaI C'yCGr_G
Cuts at: 1870 1943 2367 2704 2926 3804 4319 1870
Size: 73 424 337 222 878 515 4545
Fragments arranged by size:
4545 878 515 424 337 222 73
BamHI G'GATC_C
Cuts at: 4324 4691 4324
Size: 367 6627
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3891 4315 5770 618
Size: 325 217 627 2104 424 1455 1842
Fragments arranged by size:
2104 1842 1455 627 424 325 217
BanII G_rGCy'C
Cuts at: 1391 2357 3668 3930 3970 4313 1391
Size: 966 1311 262 40 343 4072
Fragments arranged by size:
4072 1311 966 343 262 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 2284 2380 3430 4149 928
Size: 971 122 263 96 1050 719 3773
Fragments arranged by size:
3773 1050 971 719 263 122 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4244 4278 5185 5219 1903
Size: 34 2307 34 907 34 3678
Fragments arranged by size:
3678 2307 907 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3424 4886 6096 3424
Size: 1462 1210 4322
Fragments arranged by size:
4322 1462 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6994
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 5605 136
Size: 107 122 71 631 605 700 3233 1525
Fragments arranged by size:
3233 1525 700 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3340 3392 6989 3340
Size: 52 3597 3345
Fragments arranged by size:
3597 3345 52
BlpI GC'TnA_GC
Cuts at: 3827 4411 3827
Size: 584 6410
Bme1580I G_kGCm'C
Cuts at: 1790 2149 2925 3557 3660 3970 4252 5055 6211
Size: 359 776 632 103 310 282 803 1156
Cuts at: 6211 1790
Size: 2573
Fragments arranged by size:
2573 1156 803 776 632 359 310 282
103
BmgBI CAC'GTC
Cuts at: 1318 2467 1318
Size: 1149 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2136 2836 3441
Size: 416 53 83 399 24 719 700 605
Cuts at: 3441 3525 3625 3924 4725 5683 442
Size: 84 100 299 801 958 1753
Fragments arranged by size:
1753 958 801 719 700 605 416 399
299 100 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2855 3629 3682 3731 3858 5654 1239
Size: 1616 774 53 49 127 1796 2579
Fragments arranged by size:
2579 1796 1616 774 127 53 49
Bpu10I CC'TnA_GC
Cuts at: 2192 3669 2192
Size: 1477 5517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5119 5984 6282 5119
Size: 865 298 5831
Fragments arranged by size:
5831 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 5657 882
Size: 617 2499 1659 2219
Fragments arranged by size:
2499 2219 1659 617
BsaAI yAC'GTr
Cuts at: 493 1204 2731 3235 3281 4785 493
Size: 711 1527 504 46 1504 2702
Fragments arranged by size:
2702 1527 1504 711 504 46
BsaBI GATnn'nnATC
Cuts at: 6988 6988
Size: 6994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2509 4801 5183 5818
Size: 53 83 186 936 976 2292 382 635
Cuts at: 5818 275
Size: 1451
Fragments arranged by size:
2292 1451 976 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5427 5825 6099 6246 6657 5427
Size: 398 274 147 411 5764
Fragments arranged by size:
5764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2629 2659 2828 2858 1178
Size: 30 165 30 1226 30 169 30 5314
Fragments arranged by size:
5314 1226 169 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2216 2989 3734 3935 3938 1186
Size: 835 195 773 745 201 3 4242
Fragments arranged by size:
4242 835 773 745 201 195 3
BseYI C'CCAG_C
Cuts at: 2108 2148 2232 2239 2406 2711 2829 3133 3882
Size: 40 84 7 167 305 118 304 749
Cuts at: 3882 3909 4196 6197 6693 2108
Size: 27 287 2001 496 2409
Fragments arranged by size:
2409 2001 749 496 305 304 287 167
118 84 40 27 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2708 1163
Size: 573 972 5449
Fragments arranged by size:
5449 972 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4444 5205 6233 1540
Size: 275 186 2443 761 1028 2301
Fragments arranged by size:
2443 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 3546 3675 5055 5140 6211 2031
Size: 1515 129 1380 85 1071 2814
Fragments arranged by size:
2814 1515 1380 1071 129 85
BsmI GAATG_Cn'
Cuts at: 3032 3497 4505 4598 3032
Size: 465 1008 93 5428
Fragments arranged by size:
5428 1008 465 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2149 2357 2925 3546 3557 3660
Size: 399 241 118 208 568 621 11 103
Cuts at: 3660 3668 3675 3930 3970 4252 4313 5055 5140
Size: 8 7 255 40 282 61 742 85
Cuts at: 5140 6211 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 621 568 399 282 255
241 208 118 103 85 61 40 11
8 7
BspEI T'CCGG_A
Cuts at: 6657 6657
Size: 6994
BspHI T'CATG_A
Cuts at: 4883 6613 4883
Size: 1730 5264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2371 3682 4349 844
Size: 1527 1311 667 3489
Fragments arranged by size:
3489 1527 1311 667
BsrBI CCG'CTC
Cuts at: 1394 1823 4881 1394
Size: 429 3058 3507
Fragments arranged by size:
3507 3058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 3147 4008 5489 5663 65
Size: 1183 874 1025 861 1481 174 1396
Fragments arranged by size:
1481 1396 1183 1025 874 861 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2852 5638 5825 1700
Size: 110 1042 2786 187 2869
Fragments arranged by size:
2869 2786 1042 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6994
BssSI C'ACGA_G
Cuts at: 2024 3737 5054 6066 2024
Size: 1713 1317 1012 2952
Fragments arranged by size:
2952 1713 1317 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3679 3688 794
Size: 2885 9 4100
Fragments arranged by size:
4100 2885 9
BstBI TT'CG_AA
Cuts at: 5798 5798
Size: 6994
BstEII G'GTnAC_C
Cuts at: 1184 3694 3797 1184
Size: 2510 103 4381
Fragments arranged by size:
4381 2510 103
BstXI CCAn_nnnn'nTGG
Cuts at: 2579 3235 3449 4196 2579
Size: 656 214 747 5377
Fragments arranged by size:
5377 747 656 214
BstYI r'GATC_y
Cuts at: 1972 3048 3340 3392 3589 4324 4691 5076 5093
Size: 1076 292 52 197 735 367 385 17
Cuts at: 5093 6545 6631 6643 6989 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1076 735 385 367 346 292
197 86 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 2650 3202 3477 3648 3864 4273
Size: 552 1575 10 552 275 171 216 409
Cuts at: 4273 513
Size: 3234
Fragments arranged by size:
3234 1575 552 552 409 275 216 171
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6255
Fragments arranged by size:
6255 683 56
BtsI GCAGTG_nn'
Cuts at: 2438 4510 5304 5324 2438
Size: 2072 794 20 4108
Fragments arranged by size:
4108 2072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6994
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2579 2614 2665 2700 529
Size: 35 2015 35 51 35 4823
Fragments arranged by size:
4823 2015 51 35 35 35
DraI TTT'AAA
Cuts at: 4298 4298
Size: 6994
DraIII CAC_nnn'GTG
Cuts at: 2731 2731
Size: 6994
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2288 6001 809
Size: 507 972 3713 1802
Fragments arranged by size:
3713 1802 972 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2167 2379
Size: 54 1425 50 275 18 210 127 212
Cuts at: 2379 3111 3225 3771 4441 5330 6959 8
Size: 732 114 546 670 889 1629 43
Fragments arranged by size:
1629 1425 889 732 670 546 275 212
210 127 114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4441 1537
Size: 275 2629 4090
Fragments arranged by size:
4090 2629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4924 1353
Size: 3571 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5581 5955 6101 387
Size: 1064 138 3992 374 146 1280
Fragments arranged by size:
3992 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2855 2930 2956 3629 3682 3731 3858
Size: 393 1223 75 26 673 53 49 127
Cuts at: 3858 3862 5057 5654 6441 6709 1239
Size: 4 1195 597 787 268 1524
Fragments arranged by size:
1524 1223 1195 787 673 597 393 268
127 75 53 49 26 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3101 4232 3101
Size: 1131 5863
EcoO109I rG'GnC_Cy
Cuts at: 2489 2761 2898 2963 3265 3313 3967 4200 2489
Size: 272 137 65 302 48 654 233 5283
Fragments arranged by size:
5283 654 302 272 233 137 65 48
EcoRI G'AATT_C
Cuts at: 4303 4681 4303
Size: 378 6616
FseI GG_CCGG'CC
Cuts at: 2856 2856
Size: 6994
FspI TGC'GCA
Cuts at: 5500 5500
Size: 6994
HaeII r_GCGC'y
Cuts at: 1678 2712 3576 3750 6141 1678
Size: 1034 864 174 2391 2531
Fragments arranged by size:
2531 2391 1034 864 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2629 2661 3332 3364 5635
Size: 32 135 32 1057 32 671 32 2271
Cuts at: 5635 5667 5709 5741 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2271 1057 671 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4213 4338 4584 669
Size: 988 281 2275 125 246 3079
Fragments arranged by size:
3079 2275 988 281 246 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6994
HpaI GTT'AAC
Cuts at: 4584 4584
Size: 6994
KpnI G_GTAC'C
Cuts at: 3895 4319 3895
Size: 424 6570
MfeI C'AATT_G
Cuts at: 4593 4593
Size: 6994
MluI A'CGCG_T
Cuts at: 6870 6870
Size: 6994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4144 6108 6292 1305
Size: 2839 1964 184 2007
Fragments arranged by size:
2839 2007 1964 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 2381 3113 3227 3773
Size: 54 1425 343 337 212 732 114 546
Cuts at: 3773 10
Size: 3231
Fragments arranged by size:
3231 1425 732 546 343 337 212 114
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3233 3447 3653 3821 4952 5311 5470
Size: 648 2067 214 206 168 1131 359 159
Cuts at: 5470 518
Size: 2042
Fragments arranged by size:
2067 2042 1131 648 359 214 206 168
159
MspA1I CmG'CkG
Cuts at: 1606 2179 2236 2311 2778 2833 3679 3847 3882
Size: 573 57 75 467 55 846 168 35
Cuts at: 3882 3898 3913 4043 4410 5087 6235 6480 1606
Size: 16 15 130 367 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 677 573 467 367 245
168 130 75 57 55 35 16 15
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 6994
NcoI C'CATG_G
Cuts at: 513 1065 3648 3864 513
Size: 552 2583 216 3643
Fragments arranged by size:
3643 2583 552 216
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6994
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 6994
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6994
NotI GC'GGCC_GC
Cuts at: 4441 4441
Size: 6994
NspI r_CATG'y
Cuts at: 1132 1229 2461 4067 4352 5897 1132
Size: 97 1232 1606 285 1545 2229
Fragments arranged by size:
2229 1606 1545 1232 285 97
PasI CC'CwG_GG
Cuts at: 1348 3550 1348
Size: 2202 4792
PciI A'CATG_T
Cuts at: 5893 5893
Size: 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2885 1108
Size: 1131 646 5217
Fragments arranged by size:
5217 1131 646
PmeI GTTT'AAAC
Cuts at: 4298 4298
Size: 6994
PmlI CAC'GTG
Cuts at: 2731 2731
Size: 6994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 6576 6608 5063
Size: 32 1481 32 5449
Fragments arranged by size:
5449 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2898 2898
Size: 6994
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6994
PsiI TTA'TAA
Cuts at: 4564 4564
Size: 6994
PspOMI G'GGCC_C
Cuts at: 3966 3966
Size: 6994
PstI C_TGCA'G
Cuts at: 830 2329 3684 3693 4346 830
Size: 1499 1355 9 653 3478
Fragments arranged by size:
3478 1499 1355 653 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6994
PvuII CAG'CTG
Cuts at: 2179 2311 2833 3679 3882 3898 4043 2179
Size: 132 522 846 203 16 145 5130
Fragments arranged by size:
5130 846 522 203 145 132 16
SalI G'TCGA_C
Cuts at: 1936 4336 1936
Size: 2400 4594
SbfI CC_TGCA'GG
Cuts at: 4346 4346
Size: 6994
ScaI AGT'ACT
Cuts at: 5242 6954 5242
Size: 1712 5282
SexAI A'CCwGG_T
Cuts at: 2625 2678 2625
Size: 53 6941
SfcI C'TryA_G
Cuts at: 826 1045 2325 2945 3680 3689 4342 5477 6158
Size: 219 1280 620 735 9 653 1135 681
Cuts at: 6158 6349 826
Size: 191 1471
Fragments arranged by size:
1471 1280 1135 735 681 653 620 219
191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6994
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6994
SmaI CCC'GGG
Cuts at: 1872 2369 2928 3806 4321 1872
Size: 497 559 878 515 4545
Fragments arranged by size:
4545 878 559 515 497
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5098 5999 6261 820
Size: 197 926 3155 901 262 1553
Fragments arranged by size:
3155 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4785 493
Size: 4292 2702
SpeI A'CTAG_T
Cuts at: 152 3079 152
Size: 2927 4067
SphI G_CATG'C
Cuts at: 1132 4352 1132
Size: 3220 3774
SspI AAT'ATT
Cuts at: 5 52 4918 5
Size: 47 4866 2081
Fragments arranged by size:
4866 2081 47
StuI AGG'CCT
Cuts at: 1517 3107 1517
Size: 1590 5404
StyI C'CwwG_G
Cuts at: 513 1065 2061 2153 2319 2959 3044 3067 3108
Size: 552 996 92 166 640 85 23 41
Cuts at: 3108 3648 3864 3962 4433 513
Size: 540 216 98 471 3074
Fragments arranged by size:
3074 996 640 552 540 471 216 166
98 92 85 41 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 4024 5021 5038 5191 5376 5821
Size: 1255 2001 443 997 17 153 185 445
Cuts at: 5821 325
Size: 1498
Fragments arranged by size:
2001 1498 1255 997 445 443 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 5240 6952
Size: 274 80 33 51 1443 1206 2057 1712
Cuts at: 6952 96
Size: 138
Fragments arranged by size:
2057 1712 1443 1206 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2976 3008 6954 6986 2976
Size: 32 3946 32 2984
Fragments arranged by size:
3946 2984 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6994
XbaI T'CTAG_A
Cuts at: 4330 4330
Size: 6994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 6994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6994
XmaI C'CCGG_G
Cuts at: 1870 2367 2926 3804 4319 1870
Size: 497 559 878 515 4545
Fragments arranged by size:
4545 878 559 515 497
XmnI GAAnn'nnTTC
Cuts at: 2795 5123 2795
Size: 2328 4666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4802 276
Size: 53 83 186 936 3268 2468
Fragments arranged by size:
3268 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI