Restriction map for
pFN21AE5784
(Circular) (Six-base) MAPSORT of: pFN21AE5784.seq Check: 2265 from: 1 to: 6799
pFN21AE5784 6799 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4609 278
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4142 1937
Size: 2205 4594
Acc65I G'GTAC_C
Cuts at: 4120 4120
Size: 6799
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2004 2060 2696 3155 4862 6246 6514 1632
Size: 372 56 636 459 1707 1384 268 1917
Fragments arranged by size:
1917 1707 1384 636 459 372 268 56
AfeI AGC'GCT
Cuts at: 3792 3792
Size: 6799
AflII C'TTAA_G
Cuts at: 820 1017 3419 820
Size: 197 2402 4200
Fragments arranged by size:
4200 2402 197
AflIII A'CryG_T
Cuts at: 1203 2493 3633 5698 6675 1203
Size: 1290 1140 2065 977 1327
Fragments arranged by size:
2065 1327 1290 1140 977
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 5528 5528
Size: 6799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2774 1184
Size: 1590 5209
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2800 2832 2800
Size: 32 6767
AlwNI CAG_nnn'CTG
Cuts at: 2866 3025 3178 3236 3752 3820 4542 6114 2866
Size: 159 153 58 516 68 722 1572 3551
Fragments arranged by size:
3551 1572 722 516 159 153 68 58
ApaLI G'TGCA_C
Cuts at: 4856 6012 4856
Size: 1156 5643
ApoI r'AATT_y
Cuts at: 1493 2154 2264 4108 4334 4486 6595 1493
Size: 661 110 1844 226 152 2109 1697
Fragments arranged by size:
2109 1844 1697 661 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 4082 4124 1870
Size: 73 2139 42 4545
Fragments arranged by size:
4545 2139 73 42
AvrII C'CTAG_G
Cuts at: 2399 2399
Size: 6799
BamHI G'GATC_C
Cuts at: 4129 4496 4129
Size: 367 6432
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2750 3583 4120 5575 618
Size: 325 217 627 963 833 537 1455 1842
Fragments arranged by size:
1842 1455 963 833 627 537 325 217
BanII G_rGCy'C
Cuts at: 1391 3098 3552 3737 4118 1391
Size: 1707 454 185 381 4072
Fragments arranged by size:
4072 1707 454 381 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4083 928
Size: 971 2184 3644
Fragments arranged by size:
3644 2184 971
BbvCI CC'TCA_GC
Cuts at: 3295 3506 3588 3295
Size: 211 82 6506
Fragments arranged by size:
6506 211 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4990 5024 1903
Size: 34 3053 34 3678
Fragments arranged by size:
3678 3053 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2522 4691 5901 2522
Size: 2169 1210 3420
Fragments arranged by size:
3420 2169 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5410 136
Size: 107 122 71 631 605 3738 1525
Fragments arranged by size:
3738 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6794 6794
Size: 6799
BlpI GC'TnA_GC
Cuts at: 3717 4216 3717
Size: 499 6300
Bme1580I G_kGCm'C
Cuts at: 1790 4860 6016 1790
Size: 3070 1156 2573
Fragments arranged by size:
3070 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6799
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3406 3740 4530
Size: 416 53 83 399 24 1989 334 790
Cuts at: 4530 5488 442
Size: 958 1753
Fragments arranged by size:
1989 1753 958 790 416 399 334 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3571 3571
Size: 6799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2327 2647 2988 3668 5459 1239
Size: 1046 42 320 341 680 1791 2579
Fragments arranged by size:
2579 1791 1046 680 341 320 42
Bpu10I CC'TnA_GC
Cuts at: 2705 3295 3506 3588 2705
Size: 590 211 82 5916
Fragments arranged by size:
5916 590 211 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2631 4924 5789 6087 2243
Size: 388 2293 865 298 2955
Fragments arranged by size:
2955 2293 865 388 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2913 3101 5462 882
Size: 617 1414 188 2361 2219
Fragments arranged by size:
2361 2219 1414 617 188
BsaAI yAC'GTr
Cuts at: 493 1204 2912 4590 493
Size: 711 1708 1678 2702
Fragments arranged by size:
2702 1708 1678 711
BsaBI GATnn'nnATC
Cuts at: 6793 6793
Size: 6799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4606 4988 5623 275
Size: 53 83 186 936 3073 382 635 1451
Fragments arranged by size:
3073 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5232 5630 5904 6051 6462 5232
Size: 398 274 147 411 5569
Fragments arranged by size:
5569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3186 3216 3666 3696 1178
Size: 30 165 30 1783 30 450 30 4281
Fragments arranged by size:
4281 1783 450 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3542 1186
Size: 2356 4443
BseYI C'CCAG_C
Cuts at: 2923 3242 6002 6498 2923
Size: 319 2760 496 3224
Fragments arranged by size:
3224 2760 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3141 3749 3890 1163
Size: 573 1405 608 141 4072
Fragments arranged by size:
4072 1405 608 573 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4249 5010 6038 1540
Size: 275 186 2248 761 1028 2301
Fragments arranged by size:
2301 2248 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2909 3061 3098 3552 4860 4945 6016 2909
Size: 152 37 454 1308 85 1071 3692
Fragments arranged by size:
3692 1308 1071 454 152 85 37
BsmI GAATG_Cn'
Cuts at: 2870 3165 4310 4403 2870
Size: 295 1145 93 5266
Fragments arranged by size:
5266 1145 295 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3208 3208
Size: 6799
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2909 3061 3098 3552 3737 4118 4860
Size: 399 1119 152 37 454 185 381 742
Cuts at: 4860 4945 6016 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1119 1071 742 454 399 381 185
152 85 37
BspEI T'CCGG_A
Cuts at: 6462 6462
Size: 6799
BspHI T'CATG_A
Cuts at: 3936 4688 6418 3936
Size: 752 1730 4317
Fragments arranged by size:
4317 1730 752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2676 4154 844
Size: 1832 1478 3489
Fragments arranged by size:
3489 1832 1478
BsrBI CCG'CTC
Cuts at: 1394 1823 4686 1394
Size: 429 2863 3507
Fragments arranged by size:
3507 2863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2285 5294 5468 65
Size: 1183 1037 3009 174 1396
Fragments arranged by size:
3009 1396 1183 1037 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5443 5630 1700
Size: 110 3633 187 2869
Fragments arranged by size:
3633 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2992 3630 3636 96
Size: 2896 638 6 3259
Fragments arranged by size:
3259 2896 638 6
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6799
BssSI C'ACGA_G
Cuts at: 4859 5871 4859
Size: 1012 5787
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3236 794
Size: 2442 4357
BstBI TT'CG_AA
Cuts at: 2061 5603 2061
Size: 3542 3257
BstEII G'GTnAC_C
Cuts at: 1184 3386 1184
Size: 2202 4597
BstXI CCAn_nnnn'nTGG
Cuts at: 2966 2966
Size: 6799
BstYI r'GATC_y
Cuts at: 1972 4129 4496 4881 4898 6350 6436 6448 6794
Size: 2157 367 385 17 1452 86 12 346
Cuts at: 6794 1972
Size: 1977
Fragments arranged by size:
2157 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2804 3117 3302 2804
Size: 313 185 6301
Fragments arranged by size:
6301 313 185
BtgI C'CryG_G
Cuts at: 513 1065 4059 513
Size: 552 2994 3253
Fragments arranged by size:
3253 2994 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6060
Fragments arranged by size:
6060 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3943 4315 5109 5129 3013
Size: 930 372 794 20 4683
Fragments arranged by size:
4683 930 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6764
DraI TTT'AAA
Cuts at: 2308 4103 2308
Size: 1795 5004
DraIII CAC_nnn'GTG
Cuts at: 2249 3181 3949 2249
Size: 932 768 5099
Fragments arranged by size:
5099 932 768
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5806 809
Size: 507 4490 1802
Fragments arranged by size:
4490 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4246 5135 6764
Size: 54 1425 50 275 18 2416 889 1629
Cuts at: 6764 8
Size: 43
Fragments arranged by size:
2416 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4246 1537
Size: 275 2434 4090
Fragments arranged by size:
4090 2434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 3376 3938 4026 4729 1353
Size: 1239 784 562 88 703 3423
Fragments arranged by size:
3423 1239 784 703 562 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3581 3883 5386 5760 5906 387
Size: 1064 138 1992 302 1503 374 146 1280
Fragments arranged by size:
1992 1503 1280 1064 374 302 146 138
EcoICRI GAG'CTC
Cuts at: 3096 3550 3096
Size: 454 6345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2060 2285 2327 2647 2696 2988
Size: 393 372 56 225 42 320 49 292
Cuts at: 2988 3155 3668 4862 5459 6246 6514 1239
Size: 167 513 1194 597 787 268 1524
Fragments arranged by size:
1524 1194 787 597 513 393 372 320
292 268 225 167 56 49 42
EcoO109I rG'GnC_Cy
Cuts at: 2943 3022 3842 3955 2943
Size: 79 820 113 5787
Fragments arranged by size:
5787 820 113 79
EcoRI G'AATT_C
Cuts at: 2264 4108 4486 2264
Size: 1844 378 4577
Fragments arranged by size:
4577 1844 378
FspI TGC'GCA
Cuts at: 5305 5305
Size: 6799
HaeII r_GCGC'y
Cuts at: 1678 3794 5946 1678
Size: 2116 2152 2531
Fragments arranged by size:
2531 2152 2116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3186 3218 3278 3310 5440
Size: 32 135 32 1614 32 60 32 2130
Cuts at: 5440 5472 5514 5546 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2130 1614 135 60 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 4143 4389 669
Size: 988 281 682 1523 246 3079
Fragments arranged by size:
3079 1523 988 682 281 246
HindIII A'AGCT_T
Cuts at: 748 2611 3719 748
Size: 1863 1108 3828
Fragments arranged by size:
3828 1863 1108
HpaI GTT'AAC
Cuts at: 4389 4389
Size: 6799
KpnI G_GTAC'C
Cuts at: 4124 4124
Size: 6799
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3783 5913 6097 1305
Size: 2478 2130 184 2007
Fragments arranged by size:
2478 2130 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4977
Fragments arranged by size:
4977 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4757 5116 5275 518
Size: 648 2634 957 359 159 2042
Fragments arranged by size:
2634 2042 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2625 3041 3192 3580 3688 4061 4215 4892
Size: 1019 416 151 388 108 373 154 677
Cuts at: 4892 6040 6285 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1019 677 416 388 373 245
154 151 108
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6247
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6799
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6799
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 2439 2497 3637 3997 4157 5702 1132
Size: 97 1210 58 1140 360 160 1545 2229
Fragments arranged by size:
2229 1545 1210 1140 360 160 97 58
PasI CC'CwG_GG
Cuts at: 1348 2693 1348
Size: 1345 5454
PciI A'CATG_T
Cuts at: 2493 3633 5698 2493
Size: 1140 2065 3594
Fragments arranged by size:
3594 2065 1140
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2450 2774 3752 1108
Size: 1342 324 978 4155
Fragments arranged by size:
4155 1342 978 324
PfoI T'CCnGG_A
Cuts at: 2965 3135 2965
Size: 170 6629
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PmlI CAC'GTG
Cuts at: 2912 2912
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2025 2057 2716 2748 4868 4900 6381 6413 2025
Size: 32 659 32 2120 32 1481 32 2411
Fragments arranged by size:
2411 2120 1481 659 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3842 3955 3842
Size: 113 6686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6799
PsiI TTA'TAA
Cuts at: 2423 4369 2423
Size: 1946 4853
PstI C_TGCA'G
Cuts at: 830 2763 3030 3379 4151 830
Size: 1933 267 349 772 3478
Fragments arranged by size:
3478 1933 772 349 267
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6799
PvuII CAG'CTG
Cuts at: 2625 3041 3580 3688 2625
Size: 416 539 108 5736
Fragments arranged by size:
5736 539 416 108
SacI G_AGCT'C
Cuts at: 3098 3552 3098
Size: 454 6345
SacII CC_GC'GG
Cuts at: 4062 4062
Size: 6799
SalI G'TCGA_C
Cuts at: 1936 4141 1936
Size: 2205 4594
SanDI GG'GwC_CC
Cuts at: 3842 3842
Size: 6799
SapI GCTCTTCn'nnn_
Cuts at: 3938 3938
Size: 6799
SbfI CC_TGCA'GG
Cuts at: 3030 4151 3030
Size: 1121 5678
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SexAI A'CCwGG_T
Cuts at: 3177 3972 3177
Size: 795 6004
SfcI C'TryA_G
Cuts at: 826 1045 2759 3026 3375 4147 5282 5963 6154
Size: 219 1714 267 349 772 1135 681 191
Cuts at: 6154 826
Size: 1471
Fragments arranged by size:
1714 1471 1135 772 681 349 267 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 4126 1872
Size: 2254 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2646 3419 4903 5804 6066
Size: 197 926 279 424 773 1484 901 262
Cuts at: 6066 820
Size: 1553
Fragments arranged by size:
1553 1484 926 901 773 424 279 262
197
SnaBI TAC'GTA
Cuts at: 493 4590 493
Size: 4097 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 3997 4157 1132
Size: 2865 160 3774
Fragments arranged by size:
3774 2865 160
SspI AAT'ATT
Cuts at: 5 52 4723 5
Size: 47 4671 2081
Fragments arranged by size:
4671 2081 47
StuI AGG'CCT
Cuts at: 1517 2802 1517
Size: 1285 5514
StyI C'CwwG_G
Cuts at: 513 1065 2399 2784 3322 3666 3848 4238 513
Size: 552 1334 385 538 344 182 390 3074
Fragments arranged by size:
3074 1334 552 538 390 385 344 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3348 3869 4826 4843 4996 5181 5626
Size: 1255 1768 521 957 17 153 185 445
Cuts at: 5626 325
Size: 1498
Fragments arranged by size:
1768 1498 1255 957 521 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2992 3630 3636
Size: 274 80 33 51 1443 1015 638 6
Cuts at: 3636 5045 6757 96
Size: 1409 1712 138
Fragments arranged by size:
1712 1443 1409 1015 638 274 138 80
51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3666 3698 6759 6791 3113
Size: 32 521 32 3061 32 3121
Fragments arranged by size:
3121 3061 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2521 1316
Size: 1205 5594
XbaI T'CTAG_A
Cuts at: 2191 4135 2191
Size: 1944 4855
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 4124 1870
Size: 2254 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 2127 2187 4928 2040
Size: 87 60 2741 3911
Fragments arranged by size:
3911 2741 87 60
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4607 276
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SfiI SfoI SrfI SwaI XcmI