Restriction map for
pFN21AE5782
(Circular) (Six-base) MAPSORT of: pFN21AE5782.seq Check: 2480 from: 1 to: 6757
pFN21AE5782 6757 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3627 4100 1937
Size: 1690 473 4594
Fragments arranged by size:
4594 1690 473
Acc65I G'GTAC_C
Cuts at: 4078 4078
Size: 6757
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2574 4820 6204 6472 1632
Size: 710 232 2246 1384 268 1917
Fragments arranged by size:
2246 1917 1384 710 268 232
AflII C'TTAA_G
Cuts at: 820 1017 3290 820
Size: 197 2273 4287
Fragments arranged by size:
4287 2273 197
AflIII A'CryG_T
Cuts at: 1203 5656 6633 1203
Size: 4453 977 1327
Fragments arranged by size:
4453 1327 977
AgeI A'CCGG_T
Cuts at: 2704 5588 2704
Size: 2884 3873
AhdI GACnn_n'nnGTC
Cuts at: 3179 5486 3179
Size: 2307 4450
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 4500 6072 4500
Size: 1572 5185
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 3777 4066 4292 4444 6553 1493
Size: 2284 289 226 152 2109 1697
Fragments arranged by size:
2284 2109 1697 289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2306 3029 5311 160
Size: 872 1274 723 2282 1606
Fragments arranged by size:
2282 1606 1274 872 723
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 4082 1870
Size: 73 2139 4545
Fragments arranged by size:
4545 2139 73
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4078 5533 618
Size: 325 217 627 2291 1455 1842
Fragments arranged by size:
2291 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3754 4076 1391
Size: 2363 322 4072
Fragments arranged by size:
4072 2363 322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5786
BbvCI CC'TCA_GC
Cuts at: 2726 2726
Size: 6757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4948 4982 1903
Size: 34 3011 34 3678
Fragments arranged by size:
3678 3011 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4649 5859 4649
Size: 1210 5547
BclI T'GATC_A
Cuts at: 1548 2233 2260 1548
Size: 685 27 6045
Fragments arranged by size:
6045 685 27
BfrBI ATG'CAT
Cuts at: 2881 2933 3926 2881
Size: 52 993 5712
Fragments arranged by size:
5712 993 52
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5368 136
Size: 107 122 71 631 605 3696 1525
Fragments arranged by size:
3696 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2182 3368 6752 2182
Size: 1186 3384 2187
Fragments arranged by size:
3384 2187 1186
BlpI GC'TnA_GC
Cuts at: 4174 4174
Size: 6757
Bme1580I G_kGCm'C
Cuts at: 1790 3285 4818 5974 1790
Size: 1495 1533 1156 2573
Fragments arranged by size:
2573 1533 1495 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3640 4488 5446
Size: 416 53 83 399 24 2223 848 958
Cuts at: 5446 442
Size: 1753
Fragments arranged by size:
2223 1753 958 848 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2562 2777 3024 3171 5417 1239
Size: 1323 215 247 147 2246 2579
Fragments arranged by size:
2579 2246 1323 247 215 147
Bpu10I CC'TnA_GC
Cuts at: 2726 2726
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3983 4882 5747 6045 2651
Size: 1332 899 865 298 3363
Fragments arranged by size:
3363 1332 899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3347 5420 882
Size: 617 1848 2073 2219
Fragments arranged by size:
2219 2073 1848 617
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 2061 3677 6751 2061
Size: 1616 3074 2067
Fragments arranged by size:
3074 2067 1616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4564 4946 5581 275
Size: 53 83 186 936 3031 382 635 1451
Fragments arranged by size:
3031 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2704 5190 5588 5862 6009 6420 2704
Size: 2486 398 274 147 411 3041
Fragments arranged by size:
3041 2486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2274 2304 1178
Size: 30 165 30 871 30 5631
Fragments arranged by size:
5631 871 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6757
BseYI C'CCAG_C
Cuts at: 2698 2816 3444 3988 5960 6456 2698
Size: 118 628 544 1972 496 2999
Fragments arranged by size:
2999 1972 628 544 496 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2399 1163
Size: 573 624 39 5521
Fragments arranged by size:
5521 624 573 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 4968 5996 1540
Size: 275 186 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4818 4903 5974 4818
Size: 85 1071 5601
Fragments arranged by size:
5601 1071 85
BsmI GAATG_Cn'
Cuts at: 3928 4268 4361 3928
Size: 340 93 6324
Fragments arranged by size:
6324 340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3285 3754 4076 4818 4903 5974 1391
Size: 399 1495 469 322 742 85 1071 2174
Fragments arranged by size:
2174 1495 1071 742 469 399 322 85
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 4646 6376 4646
Size: 1730 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3290 4112 844
Size: 2446 822 3489
Fragments arranged by size:
3489 2446 822
BsrBI CCG'CTC
Cuts at: 1394 1823 4644 1394
Size: 429 2821 3507
Fragments arranged by size:
3507 2821 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 3576 5252 5426 65
Size: 1183 1362 966 1676 174 1396
Fragments arranged by size:
1676 1396 1362 1183 966 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2704 5401 5588 1700
Size: 110 894 2697 187 2869
Fragments arranged by size:
2869 2697 894 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 3162 4817 5829 3162
Size: 1655 1012 4090
Fragments arranged by size:
4090 1655 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6757
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 3554 1184
Size: 2370 4387
BstYI r'GATC_y
Cuts at: 1972 2182 3368 3503 4087 4454 4839 4856 6308
Size: 210 1186 135 584 367 385 17 1452
Cuts at: 6308 6394 6406 6752 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1186 584 385 367 346 210
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 4291
Fragments arranged by size:
4291 975 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2442 487
Size: 683 56 1216 4802
Fragments arranged by size:
4802 1216 683 56
BtsI GCAGTG_nn'
Cuts at: 4273 5067 5087 4273
Size: 794 20 5943
Fragments arranged by size:
5943 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 3982 4061 3982
Size: 79 6678
DraIII CAC_nnn'GTG
Cuts at: 2138 2138
Size: 6757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 4204 5093
Size: 54 1425 50 275 18 241 2133 889
Cuts at: 5093 6722 8
Size: 1629 43
Fragments arranged by size:
2133 1629 1425 889 275 241 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2676 4687 1353
Size: 1323 2011 3423
Fragments arranged by size:
3423 2011 1323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 3104 5344 5718 5864 387
Size: 1064 138 1176 339 2240 374 146 1280
Fragments arranged by size:
2240 1280 1176 1064 374 339 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2562 2574 2777 3024 3171 4820
Size: 393 710 220 12 203 247 147 1649
Cuts at: 4820 5417 6204 6472 1239
Size: 597 787 268 1524
Fragments arranged by size:
1649 1524 787 710 597 393 268 247
220 203 147 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2586 2586
Size: 6757
EcoRI G'AATT_C
Cuts at: 4066 4444 4066
Size: 378 6379
EcoRV GAT'ATC
Cuts at: 3679 3679
Size: 6757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 2261
Size: 6757
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 5904 1678
Size: 4226 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3165 3197 3488 3520 3741
Size: 32 135 32 1593 32 291 32 221
Cuts at: 3741 3773 5398 5430 5472 5504 1373
Size: 32 1625 32 42 32 2626
Fragments arranged by size:
2626 1625 1593 291 221 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2109 3628 4101 4347 669
Size: 988 281 171 1519 473 246 3079
Fragments arranged by size:
3079 1519 988 473 281 246 171
HindIII A'AGCT_T
Cuts at: 748 2916 3732 748
Size: 2168 816 3773
Fragments arranged by size:
3773 2168 816
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 4082 4082
Size: 6757
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5871 6055 1305
Size: 4566 184 2007
Fragments arranged by size:
4566 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4935
Fragments arranged by size:
4935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2019 2811 2886 2932 4715 5074 5233
Size: 648 853 792 75 46 1783 359 159
Cuts at: 5233 518
Size: 2042
Fragments arranged by size:
2042 1783 853 792 648 359 159 75
46
MspA1I CmG'CkG
Cuts at: 1606 2114 4173 4850 5998 6243 1606
Size: 508 2059 677 1148 245 2120
Fragments arranged by size:
2120 2059 1148 677 508 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2979 513
Size: 552 939 975 4291
Fragments arranged by size:
4291 975 939 552
NdeI CA'TA_TG
Cuts at: 387 2841 2929 387
Size: 2454 88 4215
Fragments arranged by size:
4215 2454 88
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NruI TCG'CGA
Cuts at: 2461 2461
Size: 6757
NsiI A_TGCA'T
Cuts at: 2883 2935 3928 2883
Size: 52 993 5712
Fragments arranged by size:
5712 993 52
NspI r_CATG'y
Cuts at: 1132 1229 4115 5660 1132
Size: 97 2886 1545 2229
Fragments arranged by size:
2886 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6757
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 6339 6371 4826
Size: 32 1481 32 5212
Fragments arranged by size:
5212 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PstI C_TGCA'G
Cuts at: 830 4109 830
Size: 3279 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
PvuII CAG'CTG
Cuts at: 2114 2114
Size: 6757
SalI G'TCGA_C
Cuts at: 1936 3626 4099 1936
Size: 1690 473 4594
Fragments arranged by size:
4594 1690 473
SapI GCTCTTCn'nnn_
Cuts at: 2676 2676
Size: 6757
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 2827 5005 6717 2827
Size: 2178 1712 2867
Fragments arranged by size:
2867 2178 1712
SfcI C'TryA_G
Cuts at: 826 1045 2079 2415 3938 4105 5240 5921 6112
Size: 219 1034 336 1523 167 1135 681 191
Cuts at: 6112 826
Size: 1471
Fragments arranged by size:
1523 1471 1135 1034 681 336 219 191
167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 2704 1700
Size: 1004 5753
SmaI CCC'GGG
Cuts at: 1872 4084 1872
Size: 2212 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2630 3290 3962 4861 5762 6024
Size: 197 926 687 660 672 899 901 262
Cuts at: 6024 820
Size: 1553
Fragments arranged by size:
1553 926 901 899 687 672 660 262
197
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 4115 1132
Size: 2983 3774
SspI AAT'ATT
Cuts at: 5 52 2304 3772 4681 5
Size: 47 2252 1468 909 2081
Fragments arranged by size:
2252 2081 1468 909 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6757
StyI C'CwwG_G
Cuts at: 513 1065 2004 2979 4196 513
Size: 552 939 975 1217 3074
Fragments arranged by size:
3074 1217 975 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3573 4784 4801 4954 5139 5584 325
Size: 1255 1993 1211 17 153 185 445 1498
Fragments arranged by size:
1993 1498 1255 1211 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2825 5003 6715
Size: 274 80 33 51 1443 848 2178 1712
Cuts at: 6715 96
Size: 138
Fragments arranged by size:
2178 1712 1443 848 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 2520 2552 3786 3818 6717 6749 2274
Size: 32 214 32 1234 32 2899 32 2282
Fragments arranged by size:
2899 2282 1234 214 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 6757
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6757
XmaI C'CCGG_G
Cuts at: 1870 4082 1870
Size: 2212 4545
XmnI GAAnn'nnTTC
Cuts at: 2316 3255 4886 2316
Size: 939 1631 4187
Fragments arranged by size:
4187 1631 939
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI