Restriction map for
pFN21AE5781
(Circular) (Six-base) MAPSORT of: pFN21AE5781.seq Check: 4023 from: 1 to: 6637
pFN21AE5781 6637 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2988 3267 3980 1937
Size: 1051 279 713 4594
Fragments arranged by size:
4594 1051 713 279
Acc65I G'GTAC_C
Cuts at: 2115 3958 2115
Size: 1843 4794
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 4700 6084 6352 1632
Size: 699 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 699 268
AfeI AGC'GCT
Cuts at: 2918 2918
Size: 6637
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6440
AflIII A'CryG_T
Cuts at: 1203 5536 6513 1203
Size: 4333 977 1327
Fragments arranged by size:
4333 1327 977
AgeI A'CCGG_T
Cuts at: 5468 5468
Size: 6637
AhdI GACnn_n'nnGTC
Cuts at: 5366 5366
Size: 6637
AleI CACnn'nnGTG
Cuts at: 1166 2154 3878 1166
Size: 988 1724 3925
Fragments arranged by size:
3925 1724 988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3896 1184
Size: 2712 3925
AlwNI CAG_nnn'CTG
Cuts at: 2247 3372 4380 5952 2247
Size: 1125 1008 1572 2932
Fragments arranged by size:
2932 1572 1125 1008
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2369 2421 2567 2859 3946 4172 4324 6433
Size: 876 52 146 292 1087 226 152 2109
Cuts at: 6433 1493
Size: 1697
Fragments arranged by size:
2109 1697 1087 876 292 226 152 146
52
AseI AT'TA_AT
Cuts at: 160 1032 5191 160
Size: 872 4159 1606
Fragments arranged by size:
4159 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 3701 3962 1870
Size: 73 168 1590 261 4545
Fragments arranged by size:
4545 1590 261 168 73
BamHI G'GATC_C
Cuts at: 3967 4334 3967
Size: 367 6270
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 3032 3872 3958 5413
Size: 325 217 627 328 917 840 86 1455
Cuts at: 5413 618
Size: 1842
Fragments arranged by size:
1842 1455 917 840 627 328 325 217
86
BanII G_rGCy'C
Cuts at: 1391 3732 3956 1391
Size: 2341 224 4072
Fragments arranged by size:
4072 2341 224
BbeI G_GCGC'C
Cuts at: 3036 3036
Size: 6637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 2337 3111 928
Size: 971 364 74 774 4454
Fragments arranged by size:
4454 971 774 364 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2973 4529 5739 2973
Size: 1556 1210 3871
Fragments arranged by size:
3871 1556 1210
BclI T'GATC_A
Cuts at: 1548 2208 3588 1548
Size: 660 1380 4597
Fragments arranged by size:
4597 1380 660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5248 136
Size: 107 122 71 631 605 3576 1525
Fragments arranged by size:
3576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6632 6632
Size: 6637
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 3421 4698 5854 1790
Size: 1631 1277 1156 2573
Fragments arranged by size:
2573 1631 1277 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2884 3734 4368
Size: 416 53 83 399 24 1467 850 634
Cuts at: 4368 5326 442
Size: 958 1753
Fragments arranged by size:
1753 1467 958 850 634 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 3729 2611
Size: 1118 5519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2589 2606 5297 1239
Size: 1350 17 2691 2579
Fragments arranged by size:
2691 2579 1350 17
Bpu10I CC'TnA_GC
Cuts at: 3651 3651
Size: 6637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4762 5627 5925 4762
Size: 865 298 5474
Fragments arranged by size:
5474 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5300 882
Size: 617 3801 2219
Fragments arranged by size:
3801 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2263 4428 493
Size: 711 1059 2165 2702
Fragments arranged by size:
2702 2165 1059 711
BsaBI GATnn'nnATC
Cuts at: 2719 6631 2719
Size: 3912 2725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3033 4444 4826 5461
Size: 53 83 186 936 1500 1411 382 635
Cuts at: 5461 275
Size: 1451
Fragments arranged by size:
1500 1451 1411 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2185 5070 5468 5742 5889 6300 2185
Size: 2885 398 274 147 411 2522
Fragments arranged by size:
2885 2522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3077 3107 1178
Size: 30 165 30 1674 30 4708
Fragments arranged by size:
4708 1674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2322 3491 1186
Size: 829 307 1169 4332
Fragments arranged by size:
4332 1169 829 307
BseYI C'CCAG_C
Cuts at: 3414 3745 5840 6336 3414
Size: 331 2095 496 3715
Fragments arranged by size:
3715 2095 496 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2418 1163
Size: 573 682 5382
Fragments arranged by size:
5382 682 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2632 4087 4848 5876 1540
Size: 275 186 60 571 1455 761 1028 2301
Fragments arranged by size:
2301 1455 1028 761 571 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2040 4698 4783 5854 2040
Size: 2658 85 1071 2823
Fragments arranged by size:
2823 2658 1071 85
BsmI GAATG_Cn'
Cuts at: 2525 2727 4148 4241 2525
Size: 202 1421 93 4921
Fragments arranged by size:
4921 1421 202 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 3421 3732 3956 4698 4783 5854
Size: 399 250 1381 311 224 742 85 1071
Cuts at: 5854 1391
Size: 2174
Fragments arranged by size:
2174 1381 1071 742 399 311 250 224
85
BspEI T'CCGG_A
Cuts at: 2185 6300 2185
Size: 4115 2522
BspHI T'CATG_A
Cuts at: 3537 4526 6256 3537
Size: 989 1730 3918
Fragments arranged by size:
3918 1730 989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3992 844
Size: 3148 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 4524 1394
Size: 429 200 2501 3507
Fragments arranged by size:
3507 2501 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 5132 5306 65
Size: 1183 3884 174 1396
Fragments arranged by size:
3884 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 2631 5281 5468 1700
Size: 110 250 571 2650 187 2869
Fragments arranged by size:
2869 2650 571 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 4697 5709 4697
Size: 1012 5625
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6637
BstBI TT'CG_AA
Cuts at: 5441 5441
Size: 6637
BstEII G'GTnAC_C
Cuts at: 1184 3397 1184
Size: 2213 4424
BstXI CCAn_nnnn'nTGG
Cuts at: 3883 3883
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 3693 3967 4334 4719 4736 6188 6274 6286
Size: 1721 274 367 385 17 1452 86 12
Cuts at: 6286 6632 1972
Size: 346 1977
Fragments arranged by size:
1977 1721 1452 385 367 346 274 86
17 12
BstZ17I GTA'TAC
Cuts at: 3268 3268
Size: 6637
BtgI C'CryG_G
Cuts at: 513 1065 2004 2736 3900 513
Size: 552 939 732 1164 3250
Fragments arranged by size:
3250 1164 939 732 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5898
Fragments arranged by size:
5898 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 4153 4947 4967 2812
Size: 1341 794 20 4482
Fragments arranged by size:
4482 1341 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3154 3189 529
Size: 35 2590 35 3977
Fragments arranged by size:
3977 2590 35 35
DraI TTT'AAA
Cuts at: 3941 3941
Size: 6637
DraIII CAC_nnn'GTG
Cuts at: 2396 2468 2396
Size: 72 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 3885 4084
Size: 54 1425 50 275 18 228 1827 199
Cuts at: 4084 4973 6602 8
Size: 889 1629 43
Fragments arranged by size:
1827 1629 1425 889 275 228 199 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 4084 1537
Size: 275 246 2026 4090
Fragments arranged by size:
4090 2026 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 4567 1353
Size: 3214 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3380 3735 5224 5598 5744 387
Size: 1064 138 1791 355 1489 374 146 1280
Fragments arranged by size:
1791 1489 1280 1064 374 355 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2589 2606 4700 5297 6084 6352
Size: 393 699 258 17 2094 597 787 268
Cuts at: 6352 1239
Size: 1524
Fragments arranged by size:
2094 1524 787 699 597 393 268 258
17
EcoNI CCTnn'n_nnAGG
Cuts at: 3473 3473
Size: 6637
EcoO109I rG'GnC_Cy
Cuts at: 2435 2435
Size: 6637
EcoRI G'AATT_C
Cuts at: 3946 4324 3946
Size: 378 6259
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 2067 2920 3036 5784 1678
Size: 389 853 116 2748 2531
Fragments arranged by size:
2748 2531 853 389 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 2314 2346 2605
Size: 32 135 32 595 32 115 32 259
Cuts at: 2605 2637 3077 3109 3714 3746 5278 5310 5352
Size: 32 440 32 605 32 1532 32 42
Cuts at: 5352 5384 1373
Size: 32 2626
Fragments arranged by size:
2626 1532 605 595 440 259 135 115
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3981 4227 669
Size: 988 281 2043 246 3079
Fragments arranged by size:
3079 2043 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2575 748
Size: 1827 4810
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KasI G'GCGC_C
Cuts at: 3032 3032
Size: 6637
KpnI G_GTAC'C
Cuts at: 2119 3962 2119
Size: 1843 4794
MfeI C'AATT_G
Cuts at: 4236 4236
Size: 6637
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 3378 5751 5935 1305
Size: 1815 258 2373 184 2007
Fragments arranged by size:
2373 2007 1815 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3887 10
Size: 54 1425 343 2055 2760
Fragments arranged by size:
2760 2055 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2154 3251 3878 4595 4954 5113 518
Size: 648 988 1097 627 717 359 159 2042
Fragments arranged by size:
2042 1097 988 717 648 627 359 159
MspA1I CmG'CkG
Cuts at: 1606 3773 4053 4730 5878 6123 1606
Size: 2167 280 677 1148 245 2120
Fragments arranged by size:
2167 2120 1148 677 280 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 6637
NarI GG'CG_CC
Cuts at: 3033 3033
Size: 6637
NcoI C'CATG_G
Cuts at: 513 1065 2004 2736 513
Size: 552 939 732 4414
Fragments arranged by size:
4414 939 732 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 3995 5540 1132
Size: 97 2766 1545 2229
Fragments arranged by size:
2766 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6637
PciI A'CATG_T
Cuts at: 5536 5536
Size: 6637
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6637
PfoI T'CCnGG_A
Cuts at: 2562 2953 2562
Size: 391 6246
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 6219 6251 4706
Size: 32 1481 32 5092
Fragments arranged by size:
5092 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2435 2435
Size: 6637
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PstI C_TGCA'G
Cuts at: 830 2914 3989 830
Size: 2084 1075 3478
Fragments arranged by size:
3478 2084 1075
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
PvuII CAG'CTG
Cuts at: 3773 3773
Size: 6637
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 4885 6597 4885
Size: 1712 4925
SexAI A'CCwGG_T
Cuts at: 2701 2701
Size: 6637
SfcI C'TryA_G
Cuts at: 826 1045 2788 2910 3768 3985 5120 5801 5992
Size: 219 1743 122 858 217 1135 681 191
Cuts at: 5992 826
Size: 1471
Fragments arranged by size:
1743 1471 1135 858 681 219 217 191
122
SfoI GGC'GCC
Cuts at: 3034 3034
Size: 6637
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 2113 3964 1872
Size: 241 1851 4545
Fragments arranged by size:
4545 1851 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4741 5642 5904 820
Size: 197 926 2798 901 262 1553
Fragments arranged by size:
2798 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 3062 4561 5
Size: 47 3010 1499 2081
Fragments arranged by size:
3010 2081 1499 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6637
StyI C'CwwG_G
Cuts at: 513 1065 2004 2736 3358 4076 513
Size: 552 939 732 622 718 3074
Fragments arranged by size:
3074 939 732 718 622 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2166 2934 3793 4664 4681 4834 5019
Size: 1255 586 768 859 871 17 153 185
Cuts at: 5019 5464 325
Size: 445 1498
Fragments arranged by size:
1498 1255 871 859 768 586 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4883 6595 96
Size: 274 80 33 51 1443 2906 1712 138
Fragments arranged by size:
2906 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6597 6629 6597
Size: 32 6605
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3172 3796 3866 3172
Size: 624 70 5943
Fragments arranged by size:
5943 624 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6637
XmaI C'CCGG_G
Cuts at: 1870 2111 3962 1870
Size: 241 1851 4545
Fragments arranged by size:
4545 1851 241
XmnI GAAnn'nnTTC
Cuts at: 4766 4766
Size: 6637
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI