Restriction map for
pFN21AE5777
(Circular) (Six-base) MAPSORT of: pFN21AE5777.seq Check: 4760 from: 1 to: 6601
pFN21AE5777 6601 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3260 4411 278
Size: 53 83 186 936 1724 1151 2468
Fragments arranged by size:
2468 1724 1151 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3045 3825 3888 3944 1937
Size: 883 225 780 63 56 4594
Fragments arranged by size:
4594 883 780 225 63 56
Acc65I G'GTAC_C
Cuts at: 3922 3922
Size: 6601
AclI AA'CG_TT
Cuts at: 1563 4728 5101 1563
Size: 3165 373 3063
Fragments arranged by size:
3165 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2795 3798 4664 6048 6316 1632
Size: 1163 1003 866 1384 268 1917
Fragments arranged by size:
1917 1384 1163 1003 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6404
AflIII A'CryG_T
Cuts at: 1203 5500 6477 1203
Size: 4297 977 1327
Fragments arranged by size:
4297 1327 977
AgeI A'CCGG_T
Cuts at: 5432 5432
Size: 6601
AhdI GACnn_n'nnGTC
Cuts at: 3832 5330 3832
Size: 1498 5103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 6569
AlwNI CAG_nnn'CTG
Cuts at: 2058 3675 3737 4344 5916 2058
Size: 1617 62 607 1572 2743
Fragments arranged by size:
2743 1617 1572 607 62
ApaLI G'TGCA_C
Cuts at: 4658 5814 4658
Size: 1156 5445
ApoI r'AATT_y
Cuts at: 1493 2096 2233 2570 3910 4136 4288 6397 1493
Size: 603 137 337 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 603 337 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 5155 160
Size: 872 4123 1606
Fragments arranged by size:
4123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
AvaI C'yCGr_G
Cuts at: 1870 1943 3926 1870
Size: 73 1983 4545
Fragments arranged by size:
4545 1983 73
AvrII C'CTAG_G
Cuts at: 3089 3089
Size: 6601
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2762 2795 2762
Size: 33 6568
BamHI G'GATC_C
Cuts at: 3931 4298 3931
Size: 367 6234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 3081 3922 5377 618
Size: 325 217 627 456 838 841 1455 1842
Fragments arranged by size:
1842 1455 841 838 627 456 325 217
BanII G_rGCy'C
Cuts at: 1391 3521 3699 3920 1391
Size: 2130 178 221 4072
Fragments arranged by size:
4072 2130 221 178
BbeI G_GCGC'C
Cuts at: 3085 3085
Size: 6601
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2637 3189 928
Size: 971 738 552 4340
Fragments arranged by size:
4340 971 738 552
BbvCI CC'TCA_GC
Cuts at: 3299 3299
Size: 6601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3442 3476 3617 3651 4792 4826 1903
Size: 34 1505 34 141 34 1141 34 3678
Fragments arranged by size:
3678 1505 1141 141 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 5703 4493
Size: 1210 5391
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6601
BfrBI ATG'CAT
Cuts at: 2407 2407
Size: 6601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3227 3281 3335
Size: 107 122 71 631 605 1555 54 54
Cuts at: 3335 5212 136
Size: 1877 1525
Fragments arranged by size:
1877 1555 1525 631 605 122 107 71
54 54
BglII A'GATC_T
Cuts at: 2292 6596 2292
Size: 4304 2297
BlpI GC'TnA_GC
Cuts at: 2359 4018 2359
Size: 1659 4942
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2248 4662 5818 1790
Size: 337 121 2414 1156 2573
Fragments arranged by size:
2573 2414 1156 337 121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6601
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2076 3540 3892
Size: 416 53 83 399 24 659 1464 352
Cuts at: 3892 4332 5290 442
Size: 440 958 1753
Fragments arranged by size:
1753 1464 958 659 440 416 399 352
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 6601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3056 3363 5261 1239
Size: 1817 307 1898 2579
Fragments arranged by size:
2579 1898 1817 307
Bpu10I CC'TnA_GC
Cuts at: 3299 3353 3299
Size: 54 6547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4726 5591 5889 3535
Size: 1191 865 298 4247
Fragments arranged by size:
4247 1191 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2221 2366 3097 5264 882
Size: 617 722 145 731 2167 2219
Fragments arranged by size:
2219 2167 731 722 617 145
BsaAI yAC'GTr
Cuts at: 493 1204 4392 493
Size: 711 3188 2702
Fragments arranged by size:
3188 2702 711
BsaBI GATnn'nnATC
Cuts at: 6595 6595
Size: 6601
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3082 3257 4408 4790
Size: 53 83 186 936 1549 175 1151 382
Cuts at: 4790 5425 275
Size: 635 1451
Fragments arranged by size:
1549 1451 1151 936 635 382 186 175
83 53
BsaWI w'CCGG_w
Cuts at: 5034 5432 5706 5853 6264 5034
Size: 398 274 147 411 5371
Fragments arranged by size:
5371 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6376
Fragments arranged by size:
6376 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2696 1186
Size: 918 592 5091
Fragments arranged by size:
5091 918 592
BseYI C'CCAG_C
Cuts at: 3211 3276 3597 5804 6300 3211
Size: 65 321 2207 496 3512
Fragments arranged by size:
3512 2207 496 321 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3179 1163
Size: 573 1443 4585
Fragments arranged by size:
4585 1443 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4051 4812 5840 1540
Size: 275 186 2050 761 1028 2301
Fragments arranged by size:
2301 2050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3521 3699 3770 4662 4747 5818 3521
Size: 178 71 892 85 1071 4304
Fragments arranged by size:
4304 1071 892 178 85 71
BsmI GAATG_Cn'
Cuts at: 4112 4205 4112
Size: 93 6508
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2248 3521 3699 3770 3920 4662
Size: 399 337 121 1273 178 71 150 742
Cuts at: 4662 4747 5818 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1273 1071 742 399 337 178 150
121 85 71
BspEI T'CCGG_A
Cuts at: 6264 6264
Size: 6601
BspHI T'CATG_A
Cuts at: 4490 6220 4490
Size: 1730 4871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3956 844
Size: 3112 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4488 1394
Size: 429 2665 3507
Fragments arranged by size:
3507 2665 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3117 5096 5270 65
Size: 1183 1869 1979 174 1396
Fragments arranged by size:
1979 1869 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5245 5432 1700
Size: 110 3435 187 2869
Fragments arranged by size:
3435 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6601
BssSI C'ACGA_G
Cuts at: 2278 3002 4661 5673 2278
Size: 724 1659 1012 3206
Fragments arranged by size:
3206 1659 1012 724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6601
BstBI TT'CG_AA
Cuts at: 5405 5405
Size: 6601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 3476 3539 3476
Size: 63 6538
BstYI r'GATC_y
Cuts at: 1972 2292 2560 3931 4298 4683 4700 6152 6238
Size: 320 268 1371 367 385 17 1452 86
Cuts at: 6238 6250 6596 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1371 385 367 346 320 268
86 17 12
BstZ17I GTA'TAC
Cuts at: 3889 3889
Size: 6601
BtgI C'CryG_G
Cuts at: 513 1065 2004 3469 3475 3532 3538 3568 3706
Size: 552 939 1465 6 57 6 30 138
Cuts at: 3706 513
Size: 3408
Fragments arranged by size:
3408 1465 939 552 138 57 30 6
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5862
Fragments arranged by size:
5862 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 4117 4911 4931 2040
Size: 2077 794 20 3710
Fragments arranged by size:
3710 2077 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6566
DraI TTT'AAA
Cuts at: 3905 3905
Size: 6601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5608 809
Size: 507 4292 1802
Fragments arranged by size:
4292 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3282 3336 3472
Size: 54 1425 50 275 18 1452 54 136
Cuts at: 3472 3535 3709 4048 4937 6566 8
Size: 63 174 339 889 1629 43
Fragments arranged by size:
1629 1452 1425 889 339 275 174 136
63 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4048 1537
Size: 275 2236 4090
Fragments arranged by size:
4090 2236 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 3106 3851 4531 1353
Size: 797 956 745 680 3423
Fragments arranged by size:
3423 956 797 745 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 5188 5562 5708 387
Size: 1064 138 621 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 621 374 146 138
EcoICRI GAG'CTC
Cuts at: 3519 3697 3519
Size: 178 6423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2795 3056 3363 3798 4664 5261 6048
Size: 393 1163 261 307 435 866 597 787
Cuts at: 6048 6316 1239
Size: 268 1524
Fragments arranged by size:
1524 1163 866 787 597 435 393 307
268 261
EcoO109I rG'GnC_Cy
Cuts at: 2055 2422 3074 3220 3328 3601 3679 2055
Size: 367 652 146 108 273 78 4977
Fragments arranged by size:
4977 652 367 273 146 108 78
EcoRI G'AATT_C
Cuts at: 3910 4288 3910
Size: 378 6223
EcoRV GAT'ATC
Cuts at: 2859 2992 2859
Size: 133 6468
FspI TGC'GCA
Cuts at: 5107 5107
Size: 6601
HaeII r_GCGC'y
Cuts at: 1678 3085 3532 5748 1678
Size: 1407 447 2216 2531
Fragments arranged by size:
2531 2216 1407 447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2234 3350 3382 3713
Size: 32 135 32 630 32 1116 32 331
Cuts at: 3713 3745 3818 3850 5242 5274 5316 5348 1373
Size: 32 73 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1116 630 331 135 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2800 3826 3945 4191 669
Size: 988 281 862 1026 119 246 3079
Fragments arranged by size:
3079 1026 988 862 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6601
HpaI GTT'AAC
Cuts at: 2800 4191 2800
Size: 1391 5210
KasI G'GCGC_C
Cuts at: 3081 3081
Size: 6601
KpnI G_GTAC'C
Cuts at: 3926 3926
Size: 6601
MfeI C'AATT_G
Cuts at: 2901 4200 2901
Size: 1299 5302
MluI A'CGCG_T
Cuts at: 6477 6477
Size: 6601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2554 5715 5899 1305
Size: 1249 3161 184 2007
Fragments arranged by size:
3161 2007 1249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3284 3338 3474 3537 3711
Size: 54 1425 343 1452 54 136 63 174
Cuts at: 3711 10
Size: 2900
Fragments arranged by size:
2900 1452 1425 343 174 136 63 54
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 2672 3393 3474 3492 3537 3897
Size: 648 1339 167 721 81 18 45 360
Cuts at: 3897 4559 4918 5077 518
Size: 662 359 159 2042
Fragments arranged by size:
2042 1339 721 662 648 360 359 167
159 81 45 18
MspA1I CmG'CkG
Cuts at: 1606 3280 4017 4694 5842 6087 1606
Size: 1674 737 677 1148 245 2120
Fragments arranged by size:
2120 1674 1148 737 677 245
NarI GG'CG_CC
Cuts at: 3082 3082
Size: 6601
NcoI C'CATG_G
Cuts at: 513 1065 2004 3469 3475 3532 3538 3568 3706
Size: 552 939 1465 6 57 6 30 138
Cuts at: 3706 513
Size: 3408
Fragments arranged by size:
3408 1465 939 552 138 57 30 6
6
NdeI CA'TA_TG
Cuts at: 387 3894 387
Size: 3507 3094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6601
NotI GC'GGCC_GC
Cuts at: 4048 4048
Size: 6601
NsiI A_TGCA'T
Cuts at: 2409 2409
Size: 6601
NspI r_CATG'y
Cuts at: 1132 1229 2023 3959 5504 1132
Size: 97 794 1936 1545 2229
Fragments arranged by size:
2229 1936 1545 794 97
PasI CC'CwG_GG
Cuts at: 1348 3761 1348
Size: 2413 4188
PciI A'CATG_T
Cuts at: 5500 5500
Size: 6601
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3142 3761 1108
Size: 2034 619 3948
Fragments arranged by size:
3948 2034 619
PmeI GTTT'AAAC
Cuts at: 3905 3905
Size: 6601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4670 4702 6183 6215 4670
Size: 32 1481 32 5056
Fragments arranged by size:
5056 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 2422 3074 3601 2055
Size: 367 652 527 5055
Fragments arranged by size:
5055 652 527 367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6601
PsiI TTA'TAA
Cuts at: 4171 4171
Size: 6601
PstI C_TGCA'G
Cuts at: 830 2890 3953 830
Size: 2060 1063 3478
Fragments arranged by size:
3478 2060 1063
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6601
PvuII CAG'CTG
Cuts at: 3280 3280
Size: 6601
SacI G_AGCT'C
Cuts at: 3521 3699 3521
Size: 178 6423
SalI G'TCGA_C
Cuts at: 1936 3824 3943 1936
Size: 1888 119 4594
Fragments arranged by size:
4594 1888 119
SanDI GG'GwC_CC
Cuts at: 2422 2422
Size: 6601
SbfI CC_TGCA'GG
Cuts at: 3953 3953
Size: 6601
ScaI AGT'ACT
Cuts at: 4849 6561 4849
Size: 1712 4889
SexAI A'CCwGG_T
Cuts at: 2598 2598
Size: 6601
SfcI C'TryA_G
Cuts at: 826 1045 2541 2886 3064 3949 5084 5765 5956
Size: 219 1496 345 178 885 1135 681 191
Cuts at: 5956 826
Size: 1471
Fragments arranged by size:
1496 1471 1135 885 681 345 219 191
178
SfiI GGCCn_nnn'nGGCC
Cuts at: 3281 3335 3281
Size: 54 6547
SfoI GGC'GCC
Cuts at: 3083 3083
Size: 6601
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6601
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6601
SmaI CCC'GGG
Cuts at: 1872 3928 1872
Size: 2056 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3514 4705 5606 5868 820
Size: 197 926 1571 1191 901 262 1553
Fragments arranged by size:
1571 1553 1191 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4392 493
Size: 3899 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6601
SphI G_CATG'C
Cuts at: 1132 2023 3959 1132
Size: 891 1936 3774
Fragments arranged by size:
3774 1936 891
SspI AAT'ATT
Cuts at: 5 52 4525 5
Size: 47 4473 2081
Fragments arranged by size:
4473 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 1517
Size: 1074 5527
StyI C'CwwG_G
Cuts at: 513 1065 2004 3089 3469 3475 3532 3538 3568
Size: 552 939 1085 380 6 57 6 30
Cuts at: 3568 3706 4040 513
Size: 138 334 3074
Fragments arranged by size:
3074 1085 939 552 380 334 138 57
30 6 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2517 3753 4628 4645 4798 4983
Size: 1255 665 272 1236 875 17 153 185
Cuts at: 4983 5428 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1236 875 665 445 272 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4847 6559 96
Size: 274 80 33 51 1443 2870 1712 138
Fragments arranged by size:
2870 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6561 6593 6561
Size: 32 6569
Tth111I GACn'n_nGTC
Cuts at: 1316 3188 3812 1316
Size: 1872 624 4105
Fragments arranged by size:
4105 1872 624
XbaI T'CTAG_A
Cuts at: 3937 3937
Size: 6601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3545 3545
Size: 6601
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6601
XmaI C'CCGG_G
Cuts at: 1870 3926 1870
Size: 2056 4545
XmnI GAAnn'nnTTC
Cuts at: 2144 4730 2144
Size: 2586 4015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3258 4409 276
Size: 53 83 186 936 1724 1151 2468
Fragments arranged by size:
2468 1724 1151 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BsiWI BsmBI Bsu36I DraIII
EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SrfI SwaI