Restriction map for
pFN21AE5776
(Circular) (Six-base) MAPSORT of: pFN21AE5776.seq Check: 9840 from: 1 to: 6577
pFN21AE5776 6577 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4387 278
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3920 1937
Size: 1983 4594
Acc65I G'GTAC_C
Cuts at: 3898 3898
Size: 6577
AclI AA'CG_TT
Cuts at: 1563 4704 5077 1563
Size: 3141 373 3063
Fragments arranged by size:
3141 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 3831 4640 6024 6292 1632
Size: 702 1497 809 1384 268 1917
Fragments arranged by size:
1917 1497 1384 809 702 268
AfeI AGC'GCT
Cuts at: 3056 3056
Size: 6577
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6380
AflIII A'CryG_T
Cuts at: 1203 5476 6453 1203
Size: 4273 977 1327
Fragments arranged by size:
4273 1327 977
AgeI A'CCGG_T
Cuts at: 5408 5408
Size: 6577
AhdI GACnn_n'nnGTC
Cuts at: 5306 5306
Size: 6577
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3290 3438 1184
Size: 2106 148 4323
Fragments arranged by size:
4323 2106 148
AlwNI CAG_nnn'CTG
Cuts at: 2372 4320 5892 2372
Size: 1948 1572 3057
Fragments arranged by size:
3057 1948 1572
ApaLI G'TGCA_C
Cuts at: 3275 4634 5790 3275
Size: 1359 1156 4062
Fragments arranged by size:
4062 1359 1156
ApoI r'AATT_y
Cuts at: 1493 2436 3221 3755 3886 4112 4264 6373 1493
Size: 943 785 534 131 226 152 2109 1697
Fragments arranged by size:
2109 1697 943 785 534 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 2781 5131 160
Size: 872 1749 2350 1606
Fragments arranged by size:
2350 1749 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2535 3789 3902 1870
Size: 73 72 520 1254 113 4545
Fragments arranged by size:
4545 1254 520 113 73 72
BamHI G'GATC_C
Cuts at: 3907 4274 3907
Size: 367 6210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3339 3629 3898 5353 618
Size: 325 217 627 1552 290 269 1455 1842
Fragments arranged by size:
1842 1552 1455 627 325 290 269 217
BanII G_rGCy'C
Cuts at: 1391 3896 1391
Size: 2505 4072
BbeI G_GCGC'C
Cuts at: 3343 3343
Size: 6577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2882 3753 928
Size: 971 423 560 871 3752
Fragments arranged by size:
3752 971 871 560 423
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4768 4802 1903
Size: 34 2831 34 3678
Fragments arranged by size:
3678 2831 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3298 4469 5679 3298
Size: 1171 1210 4196
Fragments arranged by size:
4196 1210 1171
BclI T'GATC_A
Cuts at: 1548 3184 1548
Size: 1636 4941
BfrBI ATG'CAT
Cuts at: 2693 2693
Size: 6577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5188 136
Size: 107 122 71 631 605 3516 1525
Fragments arranged by size:
3516 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3142 6572 3142
Size: 3430 3147
BlpI GC'TnA_GC
Cuts at: 2042 3994 2042
Size: 1952 4625
Bme1580I G_kGCm'C
Cuts at: 1790 3279 4638 5794 1790
Size: 1489 1359 1156 2573
Fragments arranged by size:
2573 1489 1359 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 2571 3266
Size: 416 53 83 399 24 654 500 695
Cuts at: 3266 4308 5266 442
Size: 1042 958 1753
Fragments arranged by size:
1753 1042 958 695 654 500 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 5237 1239
Size: 969 3029 2579
Fragments arranged by size:
3029 2579 969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4702 5567 5865 4702
Size: 865 298 5414
Fragments arranged by size:
5414 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 3564 5240 882
Size: 617 792 1273 1676 2219
Fragments arranged by size:
2219 1676 1273 792 617
BsaAI yAC'GTr
Cuts at: 493 1204 4368 493
Size: 711 3164 2702
Fragments arranged by size:
3164 2702 711
BsaBI GATnn'nnATC
Cuts at: 3189 6571 3189
Size: 3382 3195
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3340 4384 4766 5401
Size: 53 83 186 936 1807 1044 382 635
Cuts at: 5401 275
Size: 1451
Fragments arranged by size:
1807 1451 1044 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5010 5408 5682 5829 6240 5010
Size: 398 274 147 411 5347
Fragments arranged by size:
5347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6352
Fragments arranged by size:
6352 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2475 3065 3153 3341 3344 1186
Size: 1051 238 590 88 188 3 4419
Fragments arranged by size:
4419 1051 590 238 188 88 3
BseYI C'CCAG_C
Cuts at: 5780 6276 5780
Size: 496 6081
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 4915
Fragments arranged by size:
4915 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4027 4788 5816 1540
Size: 275 186 2026 761 1028 2301
Fragments arranged by size:
2301 2026 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3042 3279 3532 4638 4723 5794 3042
Size: 237 253 1106 85 1071 3825
Fragments arranged by size:
3825 1106 1071 253 237 85
BsmI GAATG_Cn'
Cuts at: 2073 3241 3780 4088 4181 2073
Size: 1168 539 308 93 4469
Fragments arranged by size:
4469 1168 539 308 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3449 3449
Size: 6577
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3042 3279 3532 3896 4638 4723 5794
Size: 399 1252 237 253 364 742 85 1071
Cuts at: 5794 1391
Size: 2174
Fragments arranged by size:
2174 1252 1071 742 399 364 253 237
85
BspEI T'CCGG_A
Cuts at: 6240 6240
Size: 6577
BspHI T'CATG_A
Cuts at: 2440 2944 4466 6196 2440
Size: 504 1522 1730 2821
Fragments arranged by size:
2821 1730 1522 504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2797 3932 844
Size: 1953 1135 3489
Fragments arranged by size:
3489 1953 1135
BsrBI CCG'CTC
Cuts at: 1394 1823 2192 4464 1394
Size: 429 369 2272 3507
Fragments arranged by size:
3507 2272 429 369
BsrDI GCAATG_nn'
Cuts at: 65 1248 3520 5072 5246 65
Size: 1183 2272 1552 174 1396
Fragments arranged by size:
2272 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3336 5221 5408 1700
Size: 110 1526 1885 187 2869
Fragments arranged by size:
2869 1885 1526 187 110
BsrGI T'GTAC_A
Cuts at: 96 2972 96
Size: 2876 3701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6577
BssSI C'ACGA_G
Cuts at: 4637 5649 4637
Size: 1012 5565
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2351 2803 794
Size: 1557 452 4568
Fragments arranged by size:
4568 1557 452
BstBI TT'CG_AA
Cuts at: 5381 5381
Size: 6577
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6577
BstYI r'GATC_y
Cuts at: 1972 2010 3142 3607 3907 4274 4659 4676 6128
Size: 38 1132 465 300 367 385 17 1452
Cuts at: 6128 6214 6226 6572 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1132 465 385 367 346 300
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5086
Fragments arranged by size:
5086 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 487
Size: 683 56 1100 4738
Fragments arranged by size:
4738 1100 683 56
BtsI GCAGTG_nn'
Cuts at: 2804 2876 3432 4093 4887 4907 2804
Size: 72 556 661 794 20 4474
Fragments arranged by size:
4474 794 661 556 72 20
ClaI AT'CG_AT
Cuts at: 1554 2568 1554
Size: 1014 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6542
DraI TTT'AAA
Cuts at: 3226 3881 3226
Size: 655 5922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5584 809
Size: 507 4268 1802
Fragments arranged by size:
4268 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3009 3468 4024
Size: 54 1425 50 275 18 1179 459 556
Cuts at: 4024 4913 6542 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1179 889 556 459 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4024 1537
Size: 275 2212 4090
Fragments arranged by size:
4090 2212 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 4507 1353
Size: 966 2188 3423
Fragments arranged by size:
3423 2188 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5164 5538 5684 387
Size: 1064 138 3575 374 146 1280
Fragments arranged by size:
3575 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2334 3831 4640 5237 6024 6292
Size: 393 576 126 1497 809 597 787 268
Cuts at: 6292 1239
Size: 1524
Fragments arranged by size:
1524 1497 809 787 597 576 393 268
126
EcoO109I rG'GnC_Cy
Cuts at: 2593 2593
Size: 6577
EcoRI G'AATT_C
Cuts at: 3886 4264 3886
Size: 378 6199
FspI TGC'GCA
Cuts at: 3779 5083 3779
Size: 1304 5273
HaeII r_GCGC'y
Cuts at: 1678 3058 3343 5724 1678
Size: 1380 285 2381 2531
Fragments arranged by size:
2531 2381 1380 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2926 2958 5218 5250 5292
Size: 32 135 32 1354 32 2260 32 42
Cuts at: 5292 5324 1373
Size: 32 2626
Fragments arranged by size:
2626 2260 1354 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3921 4167 669
Size: 988 281 1983 246 3079
Fragments arranged by size:
3079 1983 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6577
HpaI GTT'AAC
Cuts at: 4167 4167
Size: 6577
KasI G'GCGC_C
Cuts at: 3339 3339
Size: 6577
KpnI G_GTAC'C
Cuts at: 3902 3902
Size: 6577
MfeI C'AATT_G
Cuts at: 4176 4176
Size: 6577
MluI A'CGCG_T
Cuts at: 6453 6453
Size: 6577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3411 3544 5691 5875 1305
Size: 2106 133 2147 184 2007
Fragments arranged by size:
2147 2106 2007 184 133
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 3470 10
Size: 54 1425 343 1179 459 3117
Fragments arranged by size:
3117 1425 1179 459 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 2496 4535 4894 5053 518
Size: 648 1279 51 2039 359 159 2042
Fragments arranged by size:
2042 2039 1279 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 3993 4670 5818 6063 1606
Size: 2387 677 1148 245 2120
Fragments arranged by size:
2387 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3340 3340
Size: 6577
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5086
Fragments arranged by size:
5086 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6577
NotI GC'GGCC_GC
Cuts at: 4024 4024
Size: 6577
NsiI A_TGCA'T
Cuts at: 2695 2695
Size: 6577
NspI r_CATG'y
Cuts at: 1132 1229 2495 3084 3290 3422 3586 3935 5480
Size: 97 1266 589 206 132 164 349 1545
Cuts at: 5480 1132
Size: 2229
Fragments arranged by size:
2229 1545 1266 589 349 206 164 132
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6577
PciI A'CATG_T
Cuts at: 5476 5476
Size: 6577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2840 3069 3364 3626 3834 1108
Size: 1732 229 295 262 208 3851
Fragments arranged by size:
3851 1732 295 262 229 208
PmeI GTTT'AAAC
Cuts at: 3881 3881
Size: 6577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 6159 6191 4646
Size: 32 1481 32 5032
Fragments arranged by size:
5032 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6577
PsiI TTA'TAA
Cuts at: 4147 4147
Size: 6577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2913 2945 2913
Size: 32 6545
PstI C_TGCA'G
Cuts at: 830 3427 3929 830
Size: 2597 502 3478
Fragments arranged by size:
3478 2597 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6577
SalI G'TCGA_C
Cuts at: 1936 3919 1936
Size: 1983 4594
SbfI CC_TGCA'GG
Cuts at: 3929 3929
Size: 6577
ScaI AGT'ACT
Cuts at: 2864 4825 6537 2864
Size: 1961 1712 2904
Fragments arranged by size:
2904 1961 1712
SexAI A'CCwGG_T
Cuts at: 2229 2229
Size: 6577
SfcI C'TryA_G
Cuts at: 826 1045 2663 2925 3423 3925 5060 5741 5932
Size: 219 1618 262 498 502 1135 681 191
Cuts at: 5932 826
Size: 1471
Fragments arranged by size:
1618 1471 1135 681 502 498 262 219
191
SfoI GGC'GCC
Cuts at: 3341 3341
Size: 6577
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6577
SmaI CCC'GGG
Cuts at: 1872 2017 2537 3904 1872
Size: 145 520 1367 4545
Fragments arranged by size:
4545 1367 520 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3789 4681 5582 5844 820
Size: 197 926 1846 892 901 262 1553
Fragments arranged by size:
1846 1553 926 901 892 262 197
SnaBI TAC'GTA
Cuts at: 493 4368 493
Size: 3875 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6577
SphI G_CATG'C
Cuts at: 1132 3422 3586 3935 1132
Size: 2290 164 349 3774
Fragments arranged by size:
3774 2290 349 164
SrfI GCCC'GGGC
Cuts at: 2537 2537
Size: 6577
SspI AAT'ATT
Cuts at: 5 52 2275 4501 5
Size: 47 2223 2226 2081
Fragments arranged by size:
2226 2223 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6577
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 3828 3864 4016 513
Size: 552 939 213 1611 36 152 3074
Fragments arranged by size:
3074 1611 939 552 213 152 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2791 3356 4604 4621 4774 4959 5404
Size: 1255 1211 565 1248 17 153 185 445
Cuts at: 5404 325
Size: 1498
Fragments arranged by size:
1498 1255 1248 1211 565 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2862 2972 4823
Size: 274 80 33 51 1443 885 110 1851
Cuts at: 4823 6535 96
Size: 1712 138
Fragments arranged by size:
1851 1712 1443 885 274 138 110 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2131 2163 2783 2815 6537 6569 2131
Size: 32 620 32 3722 32 2139
Fragments arranged by size:
3722 2139 620 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2639 1316
Size: 1323 5254
XbaI T'CTAG_A
Cuts at: 3322 3913 3322
Size: 591 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3365 3365
Size: 6577
XhoI C'TCGA_G
Cuts at: 1943 3789 1943
Size: 1846 4731
XmaI C'CCGG_G
Cuts at: 1870 2015 2535 3902 1870
Size: 145 520 1367 4545
Fragments arranged by size:
4545 1367 520 145
XmnI GAAnn'nnTTC
Cuts at: 3441 3675 4706 3441
Size: 234 1031 5312
Fragments arranged by size:
5312 1031 234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4385 276
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SfiI SwaI