Restriction map for
pFN21AE5772
(Circular) (Six-base) MAPSORT of: pFN21AE5772.seq Check: 6559 from: 1 to: 6484
pFN21AE5772 6484 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3525 3525
Size: 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3613 3805 3613
Size: 192 6292
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2310 2480 3230 4547 5931 6199 1632
Size: 596 82 170 750 1317 1384 268 1917
Fragments arranged by size:
1917 1384 1317 750 596 268 170 82
AfeI AGC'GCT
Cuts at: 2130 2130
Size: 6484
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3362 1184
Size: 2178 4306
AlwNI CAG_nnn'CTG
Cuts at: 2267 3319 4227 5799 2267
Size: 1052 908 1572 2952
Fragments arranged by size:
2952 1572 1052 908
ApaLI G'TGCA_C
Cuts at: 3726 4541 5697 3726
Size: 815 1156 4513
Fragments arranged by size:
4513 1156 815
ApoI r'AATT_y
Cuts at: 1493 3793 4019 4171 6280 1493
Size: 2300 226 152 2109 1697
Fragments arranged by size:
2300 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2702 3024 3180 3416 3450 3597 3809
Size: 73 759 322 156 236 34 147 212
Cuts at: 3809 1870
Size: 4545
Fragments arranged by size:
4545 759 322 236 212 156 147 73
34
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2669 3338 3613 3625 3805
Size: 325 217 627 882 669 275 12 180
Cuts at: 3805 5260 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 882 669 627 325 275 217
180 12
BanII G_rGCy'C
Cuts at: 1391 2301 2688 3434 3636 3803 1391
Size: 910 387 746 202 167 4072
Fragments arranged by size:
4072 910 746 387 202 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2743 928
Size: 971 844 4669
Fragments arranged by size:
4669 971 844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 3195 1548
Size: 1647 4837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2940 3044 5095
Size: 107 122 71 631 605 1268 104 2051
Cuts at: 5095 136
Size: 1525
Fragments arranged by size:
2051 1525 1268 631 605 122 107 104
71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 2867 3901 2867
Size: 1034 5450
Bme1580I G_kGCm'C
Cuts at: 1790 2672 3288 3730 3745 4545 5701 1790
Size: 882 616 442 15 800 1156 2573
Fragments arranged by size:
2573 1156 882 800 616 442 15
BmgBI CAC'GTC
Cuts at: 1318 3748 1318
Size: 2430 4054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3476
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2555 3032 3668 5144 1239
Size: 1253 63 477 636 1476 2579
Fragments arranged by size:
2579 1476 1253 636 477 63
Bpu10I CC'TnA_GC
Cuts at: 3428 3428
Size: 6484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3446 4609 5474 5772 3446
Size: 1163 865 298 4158
Fragments arranged by size:
4158 1163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3644 5147 882
Size: 617 2145 1503 2219
Fragments arranged by size:
2219 2145 1503 617
BsaAI yAC'GTr
Cuts at: 493 1204 3307 3389 4275 493
Size: 711 2103 82 886 2702
Fragments arranged by size:
2702 2103 886 711 82
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 4291 4673 5308
Size: 53 83 186 936 501 2257 382 635
Cuts at: 5308 275
Size: 1451
Fragments arranged by size:
2257 1451 936 635 501 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 1178
Size: 30 165 30 1086 30 5143
Fragments arranged by size:
5143 1086 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 1186
Size: 1324 5160
BseYI C'CCAG_C
Cuts at: 2308 2665 2738 2874 3047 5687 6183 2308
Size: 357 73 136 173 2640 496 2609
Fragments arranged by size:
2640 2609 496 357 173 136 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2996 3452 1163
Size: 573 1260 456 4195
Fragments arranged by size:
4195 1260 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3780 3934 4695 5723 1540
Size: 275 186 1779 154 761 1028 2301
Fragments arranged by size:
2301 1779 1028 761 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2217 2688 3730 4545 4630 5701 2217
Size: 471 1042 815 85 1071 3000
Fragments arranged by size:
3000 1071 1042 815 471 85
BsmI GAATG_Cn'
Cuts at: 3019 3995 4088 3019
Size: 976 93 5415
Fragments arranged by size:
5415 976 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2480 2480
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2301 2672 2688 3288 3434 3636
Size: 399 427 84 371 16 600 146 202
Cuts at: 3636 3730 3745 3803 4545 4630 5701 1391
Size: 94 15 58 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 427 399 371 202
146 94 85 84 58 16 15
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 3171 4373 6103 3171
Size: 1202 1730 3552
Fragments arranged by size:
3552 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2260 3384 3414 3525 3839 844
Size: 1416 1124 30 111 314 3489
Fragments arranged by size:
3489 1416 1124 314 111 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3328 4371 1394
Size: 429 1505 1043 3507
Fragments arranged by size:
3507 1505 1043 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2059 2815 4979 5153 65
Size: 1183 811 756 2164 174 1396
Fragments arranged by size:
2164 1396 1183 811 756 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3141 5128 5315 1700
Size: 110 1331 1987 187 2869
Fragments arranged by size:
2869 1987 1331 187 110
BsrGI T'GTAC_A
Cuts at: 96 3570 96
Size: 3474 3010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 2066 5288 2066
Size: 3222 3262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 3204 3814 4181 4566 4583 6035 6121 6133
Size: 1232 610 367 385 17 1452 86 12
Cuts at: 6133 6479 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1232 610 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2796 3474 513
Size: 552 939 792 678 3523
Fragments arranged by size:
3523 939 792 678 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 3126 3228 3317 4000 4794 4814 3126
Size: 102 89 683 794 20 4796
Fragments arranged by size:
4796 794 683 102 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 3475 3475
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2097 2767
Size: 54 1425 50 275 18 177 90 670
Cuts at: 2767 3054 3139 3777 3931 4820 6449 8
Size: 287 85 638 154 889 1629 43
Fragments arranged by size:
1629 1425 889 670 638 287 275 177
154 90 85 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3777 3931 1537
Size: 275 1965 154 4090
Fragments arranged by size:
4090 1965 275 154
EarI CTCTTCn'nnn_
Cuts at: 1353 2827 4414 1353
Size: 1474 1587 3423
Fragments arranged by size:
3423 1587 1474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2686
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2310 2480 2492 2555 3032 3230
Size: 393 596 82 170 12 63 477 198
Cuts at: 3230 3668 4547 5144 5931 6199 1239
Size: 438 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 596 477 438 393
268 198 170 82 63 12
EcoO109I rG'GnC_Cy
Cuts at: 2176 2330 2905 3028 3088 3251 3412 3484 2176
Size: 154 575 123 60 163 161 72 5176
Fragments arranged by size:
5176 575 163 161 154 123 72 60
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 6484
FspI TGC'GCA
Cuts at: 2082 4990 2082
Size: 2908 3576
FspAI rTGC'GCAy
Cuts at: 2082 2082
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2132 2874 3584 5631 1678
Size: 454 742 710 2047 2531
Fragments arranged by size:
2531 2047 742 710 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 2489 2521 2636
Size: 32 135 32 724 32 161 32 115
Cuts at: 2636 2668 5125 5157 5199 5231 1373
Size: 32 2457 32 42 32 2626
Fragments arranged by size:
2626 2457 724 161 135 115 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3751 3828 4074 669
Size: 988 281 1813 77 246 3079
Fragments arranged by size:
3079 1813 988 281 246 77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3617 3809 3617
Size: 192 6292
MfeI C'AATT_G
Cuts at: 2387 4083 2387
Size: 1696 4788
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 2601 5598 5782 1305
Size: 750 546 2997 184 2007
Fragments arranged by size:
2997 2007 750 546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2769 3056 10
Size: 54 1425 343 267 670 287 3438
Fragments arranged by size:
3438 1425 670 343 287 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2609 3344 3698 4442 4801 4960
Size: 648 982 461 735 354 744 359 159
Cuts at: 4960 518
Size: 2042
Fragments arranged by size:
2042 982 744 735 648 461 359 354
159
MspA1I CmG'CkG
Cuts at: 1606 2012 2264 2533 2798 3900 4577 5725 5970
Size: 406 252 269 265 1102 677 1148 245
Cuts at: 5970 1606
Size: 2120
Fragments arranged by size:
2120 1148 1102 677 406 269 265 252
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4993
Fragments arranged by size:
4993 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3777 3931 3777
Size: 154 6330
NspI r_CATG'y
Cuts at: 1132 1229 3757 3842 5387 1132
Size: 97 2528 85 1545 2229
Fragments arranged by size:
2528 2229 1545 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 1108
Size: 1773 4711
PfoI T'CCnGG_A
Cuts at: 2481 3009 2481
Size: 528 5956
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2905 3251 2176
Size: 729 346 5409
Fragments arranged by size:
5409 729 346
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspXI vC'TCGA_Gb
Cuts at: 3180 3416 3180
Size: 236 6248
PstI C_TGCA'G
Cuts at: 830 2532 3036 3411 3522 3836 830
Size: 1702 504 375 111 314 3478
Fragments arranged by size:
3478 1702 504 375 314 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2264 2533 2264
Size: 269 6215
SacI G_AGCT'C
Cuts at: 2688 2688
Size: 6484
SacII CC_GC'GG
Cuts at: 2799 2799
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 3251 3251
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3036 3411 3836 3036
Size: 375 425 5684
Fragments arranged by size:
5684 425 375
ScaI AGT'ACT
Cuts at: 2855 4732 6444 2855
Size: 1877 1712 2895
Fragments arranged by size:
2895 1877 1712
SfcI C'TryA_G
Cuts at: 826 1045 2528 3032 3407 3518 3832 4967 5648
Size: 219 1483 504 375 111 314 1135 681
Cuts at: 5648 5839 826
Size: 191 1471
Fragments arranged by size:
1483 1471 1135 681 504 375 314 219
191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3026 3599 3811 1872
Size: 1154 573 212 4545
Fragments arranged by size:
4545 1154 573 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3180 3416 3461 4588 5489 5751
Size: 197 926 1237 236 45 1127 901 262
Cuts at: 5751 820
Size: 1553
Fragments arranged by size:
1553 1237 1127 926 901 262 236 197
45
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 2708 1517
Size: 1191 5293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2179 3487 3923 513
Size: 552 939 175 1308 436 3074
Fragments arranged by size:
3074 1308 939 552 436 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2454 2939 2963 4511 4528 4681
Size: 1255 672 202 485 24 1548 17 153
Cuts at: 4681 4866 5311 325
Size: 185 445 1498
Fragments arranged by size:
1548 1498 1255 672 485 445 202 185
153 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2853 3570 4730
Size: 274 80 33 51 1443 876 717 1160
Cuts at: 4730 6442 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1160 876 717 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2214 2246 3231 3263 6444 6476 2214
Size: 32 985 32 3181 32 2222
Fragments arranged by size:
3181 2222 985 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3007 2281
Size: 726 5758
XhoI C'TCGA_G
Cuts at: 1943 3180 3416 1943
Size: 1237 236 5011
Fragments arranged by size:
5011 1237 236
XmaI C'CCGG_G
Cuts at: 1870 3024 3597 3809 1870
Size: 1154 573 212 4545
Fragments arranged by size:
4545 1154 573 212
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI