Restriction map for
pFN21AE5765
(Circular) (Six-base) MAPSORT of: pFN21AE5765.seq Check: 2252 from: 1 to: 7654
pFN21AE5765 7654 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5464 278
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3998 4445 4997 1937
Size: 2061 447 552 4594
Fragments arranged by size:
4594 2061 552 447
Acc65I G'GTAC_C
Cuts at: 2716 4975 2716
Size: 2259 5395
AclI AA'CG_TT
Cuts at: 1563 5781 6154 1563
Size: 4218 373 3063
Fragments arranged by size:
4218 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2303 3330 3735 3960 4115 4254 4767
Size: 539 132 1027 405 225 155 139 513
Cuts at: 4767 5717 7101 7369 1632
Size: 950 1384 268 1917
Fragments arranged by size:
1917 1384 1027 950 539 513 405 268
225 155 139 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7457
AflIII A'CryG_T
Cuts at: 1203 2925 6553 7530 1203
Size: 1722 3628 977 1327
Fragments arranged by size:
3628 1722 1327 977
AgeI A'CCGG_T
Cuts at: 2505 6485 2505
Size: 3980 3674
AhdI GACnn_n'nnGTC
Cuts at: 6383 6383
Size: 7654
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7654
AlwNI CAG_nnn'CTG
Cuts at: 4745 5397 6969 4745
Size: 652 1572 5430
Fragments arranged by size:
5430 1572 652
ApaLI G'TGCA_C
Cuts at: 5711 6867 5711
Size: 1156 6498
ApoI r'AATT_y
Cuts at: 1493 2291 2822 3538 4161 4506 4865 4963 5189
Size: 798 531 716 623 345 359 98 226
Cuts at: 5189 5341 7450 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 798 716 623 531 359 345
226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 6208 160
Size: 872 5176 1606
Fragments arranged by size:
5176 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 4979 1870
Size: 73 123 2913 4545
Fragments arranged by size:
4545 2913 123 73
BamHI G'GATC_C
Cuts at: 4984 5351 4984
Size: 367 7287
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 3719 4975 6430 618
Size: 325 217 627 929 1003 1256 1455 1842
Fragments arranged by size:
1842 1455 1256 1003 929 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 4771 4973 1391
Size: 682 2698 202 4072
Fragments arranged by size:
4072 2698 682 202
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2524 2838 3524 4322 928
Size: 971 625 314 686 798 4260
Fragments arranged by size:
4260 971 798 686 625 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 5845 5879 1903
Size: 34 110 34 3764 34 3678
Fragments arranged by size:
3764 3678 110 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5546 6756 5546
Size: 1210 6444
BclI T'GATC_A
Cuts at: 1548 2019 2167 2938 1548
Size: 471 148 771 6264
Fragments arranged by size:
6264 771 471 148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6265 136
Size: 107 122 71 631 605 4593 1525
Fragments arranged by size:
4593 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4645 4786 7649 4645
Size: 141 2863 4650
Fragments arranged by size:
4650 2863 141
BlpI GC'TnA_GC
Cuts at: 2054 5071 2054
Size: 3017 4637
Bme1580I G_kGCm'C
Cuts at: 1790 3724 5715 6871 1790
Size: 1934 1991 1156 2573
Fragments arranged by size:
2573 1991 1934 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7654
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3086 3114
Size: 416 53 83 399 24 969 700 28
Cuts at: 3114 5385 6343 442
Size: 2271 958 1753
Fragments arranged by size:
2271 1753 969 958 700 416 399 83
53 28 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2688 3513 3522 3609 4229 4434 6314
Size: 1002 447 825 9 87 620 205 1880
Cuts at: 6314 1239
Size: 2579
Fragments arranged by size:
2579 1880 1002 825 620 447 205 87
9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 5779 6644 6942 2027
Size: 3752 865 298 2739
Fragments arranged by size:
3752 2739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2496 6317 882
Size: 617 997 3821 2219
Fragments arranged by size:
3821 2219 997 617
BsaAI yAC'GTr
Cuts at: 493 1204 2857 5445 493
Size: 711 1653 2588 2702
Fragments arranged by size:
2702 2588 1653 711
BsaBI GATnn'nnATC
Cuts at: 2085 2469 2973 7648 2085
Size: 384 504 4675 2091
Fragments arranged by size:
4675 2091 504 384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5461 5843 6478 275
Size: 53 83 186 936 3928 382 635 1451
Fragments arranged by size:
3928 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2505 6087 6485 6759 6906 7317 2505
Size: 3582 398 274 147 411 2842
Fragments arranged by size:
3582 2842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2539 2551 2569 3108
Size: 30 165 30 1118 18 12 18 539
Cuts at: 3108 3138 3835 3865 4407 4437 1178
Size: 30 697 30 542 30 4395
Fragments arranged by size:
4395 1118 697 542 539 165 30 30
30 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2244 2472 2934 3021 3177 3240 1186
Size: 1058 228 462 87 156 63 5600
Fragments arranged by size:
5600 1058 462 228 156 87 63
BseYI C'CCAG_C
Cuts at: 2998 3922 6857 7353 2998
Size: 924 2935 496 3299
Fragments arranged by size:
3299 2935 924 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4035 4548 1163
Size: 573 2299 513 4269
Fragments arranged by size:
4269 2299 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5104 5865 6893 1540
Size: 275 186 3103 761 1028 2301
Fragments arranged by size:
3103 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4771 5715 5800 6871 4771
Size: 944 85 1071 5554
Fragments arranged by size:
5554 1071 944 85
BsmI GAATG_Cn'
Cuts at: 3234 5165 5258 3234
Size: 1931 93 5630
Fragments arranged by size:
5630 1931 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 3724 4771 4973 5715 5800 6871
Size: 399 283 1651 1047 202 742 85 1071
Cuts at: 6871 1391
Size: 2174
Fragments arranged by size:
2174 1651 1071 1047 742 399 283 202
85
BspEI T'CCGG_A
Cuts at: 7317 7317
Size: 7654
BspHI T'CATG_A
Cuts at: 2827 5543 7273 2827
Size: 2716 1730 3208
Fragments arranged by size:
3208 2716 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3961 5009 844
Size: 3117 1048 3489
Fragments arranged by size:
3489 3117 1048
BsrBI CCG'CTC
Cuts at: 1394 1823 3919 5541 1394
Size: 429 2096 1622 3507
Fragments arranged by size:
3507 2096 1622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3110 6149 6323 65
Size: 1183 1862 3039 174 1396
Fragments arranged by size:
3039 1862 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2505 6298 6485 1700
Size: 110 695 3793 187 2869
Fragments arranged by size:
3793 2869 695 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7654
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7654
BssSI C'ACGA_G
Cuts at: 5714 6726 5714
Size: 1012 6642
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7654
BstBI TT'CG_AA
Cuts at: 6458 6458
Size: 7654
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7654
BstXI CCAn_nnnn'nTGG
Cuts at: 4609 4609
Size: 7654
BstYI r'GATC_y
Cuts at: 1972 2086 2644 2680 4645 4786 4984 5351 5736
Size: 114 558 36 1965 141 198 367 385
Cuts at: 5736 5753 7205 7291 7303 7649 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1965 1452 558 385 367 346 198
141 114 86 36 17 12
BstZ17I GTA'TAC
Cuts at: 4446 4446
Size: 7654
Bsu36I CC'TnA_GG
Cuts at: 3987 3987
Size: 7654
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6163
Fragments arranged by size:
6163 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6915
Fragments arranged by size:
6915 683 56
BtsI GCAGTG_nn'
Cuts at: 5170 5964 5984 5170
Size: 794 20 6840
Fragments arranged by size:
6840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7619
DraI TTT'AAA
Cuts at: 4958 4958
Size: 7654
DraIII CAC_nnn'GTG
Cuts at: 3358 3358
Size: 7654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6661 809
Size: 507 5345 1802
Fragments arranged by size:
5345 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2734 4537 5101
Size: 54 1425 50 275 18 904 1803 564
Cuts at: 5101 5990 7619 8
Size: 889 1629 43
Fragments arranged by size:
1803 1629 1425 904 889 564 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5101 1537
Size: 275 3289 4090
Fragments arranged by size:
4090 3289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 2340 2482 2716 3979 4869 4917 4945
Size: 969 18 142 234 1263 890 48 28
Cuts at: 4945 5584 1353
Size: 639 3423
Fragments arranged by size:
3423 1263 969 890 639 234 142 48
28 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2083 2542 6241 6615 6761 387
Size: 1064 138 494 459 3699 374 146 1280
Fragments arranged by size:
3699 1280 1064 494 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 4769 4769
Size: 7654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2241 2303 2688 3330 3513 3522
Size: 393 539 70 62 385 642 183 9
Cuts at: 3522 3609 3735 3960 4115 4229 4254 4434 4767
Size: 87 126 225 155 114 25 180 333
Cuts at: 4767 5717 6314 7101 7369 1239
Size: 950 597 787 268 1524
Fragments arranged by size:
1524 950 787 642 597 539 393 385
333 268 225 183 180 155 126 114
87 70 62 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3135 3986 3135
Size: 851 6803
EcoO109I rG'GnC_Cy
Cuts at: 3087 3087
Size: 7654
EcoRI G'AATT_C
Cuts at: 4506 4963 5341 4506
Size: 457 378 6819
Fragments arranged by size:
6819 457 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2594 4667 2594
Size: 2073 5581
FspI TGC'GCA
Cuts at: 4665 6160 4665
Size: 1495 6159
HaeII r_GCGC'y
Cuts at: 1678 6801 1678
Size: 5123 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2539 2571 2834
Size: 32 135 32 680 32 255 32 263
Cuts at: 2834 2866 3452 3484 4407 4439 4688 4720 4919
Size: 32 586 32 923 32 249 32 199
Cuts at: 4919 4951 6295 6327 6369 6401 1373
Size: 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 923 680 586 263 255 249
199 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3685 4998 5244 669
Size: 988 281 1747 1313 246 3079
Fragments arranged by size:
3079 1747 1313 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3602 748
Size: 2854 4800
HpaI GTT'AAC
Cuts at: 3685 5244 3685
Size: 1559 6095
KpnI G_GTAC'C
Cuts at: 2720 4979 2720
Size: 2259 5395
MfeI C'AATT_G
Cuts at: 5253 5253
Size: 7654
MluI A'CGCG_T
Cuts at: 7530 7530
Size: 7654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2543 6768 6952 1305
Size: 1238 4225 184 2007
Fragments arranged by size:
4225 2007 1238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2736 10
Size: 54 1425 343 904 4928
Fragments arranged by size:
4928 1425 904 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2946 5612 5971 6130 518
Size: 648 1780 2666 359 159 2042
Fragments arranged by size:
2666 2042 1780 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 4037 4459 4754 4829 5070 5747 6895
Size: 567 1864 422 295 75 241 677 1148
Cuts at: 6895 7140 1606
Size: 245 2120
Fragments arranged by size:
2120 1864 1148 677 567 422 295 245
241 75
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6163
Fragments arranged by size:
6163 939 552
NdeI CA'TA_TG
Cuts at: 387 3286 387
Size: 2899 4755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7654
NotI GC'GGCC_GC
Cuts at: 5101 5101
Size: 7654
NspI r_CATG'y
Cuts at: 1132 1229 2929 3365 5012 6557 1132
Size: 97 1700 436 1647 1545 2229
Fragments arranged by size:
2229 1700 1647 1545 436 97
PasI CC'CwG_GG
Cuts at: 1348 4602 1348
Size: 3254 4400
PciI A'CATG_T
Cuts at: 2925 6553 2925
Size: 3628 4026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7654
PfoI T'CCnGG_A
Cuts at: 3034 4318 3034
Size: 1284 6370
PmeI GTTT'AAAC
Cuts at: 4958 4958
Size: 7654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4515 4547 5723 5755 7236 7268 4515
Size: 32 1176 32 1481 32 4901
Fragments arranged by size:
4901 1481 1176 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2928 1613
Size: 1315 6339
PsiI TTA'TAA
Cuts at: 3702 5224 3702
Size: 1522 6132
PstI C_TGCA'G
Cuts at: 830 3280 3958 4084 4759 5006 830
Size: 2450 678 126 675 247 3478
Fragments arranged by size:
3478 2450 678 675 247 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7654
PvuII CAG'CTG
Cuts at: 2173 4037 4459 4754 4829 2173
Size: 1864 422 295 75 4998
Fragments arranged by size:
4998 1864 422 295 75
SacI G_AGCT'C
Cuts at: 4771 4771
Size: 7654
SalI G'TCGA_C
Cuts at: 1936 4996 1936
Size: 3060 4594
SapI GCTCTTCn'nnn_
Cuts at: 2340 2340
Size: 7654
SbfI CC_TGCA'GG
Cuts at: 5006 5006
Size: 7654
ScaI AGT'ACT
Cuts at: 3943 5902 7614 3943
Size: 1959 1712 3983
Fragments arranged by size:
3983 1959 1712
SfcI C'TryA_G
Cuts at: 826 1045 3219 3276 3954 4080 4755 5002 6137
Size: 219 2174 57 678 126 675 247 1135
Cuts at: 6137 6818 7009 826
Size: 681 191 1471
Fragments arranged by size:
2174 1471 1135 681 678 675 247 219
191 126 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7654
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7654
SmaI CCC'GGG
Cuts at: 1872 4981 1872
Size: 3109 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2066 5758 6659 6921 820
Size: 197 926 99 24 3692 901 262 1553
Fragments arranged by size:
3692 1553 926 901 262 197 99 24
SnaBI TAC'GTA
Cuts at: 493 2857 5445 493
Size: 2364 2588 2702
Fragments arranged by size:
2702 2588 2364
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7654
SphI G_CATG'C
Cuts at: 1132 3365 5012 1132
Size: 2233 1647 3774
Fragments arranged by size:
3774 2233 1647
SspI AAT'ATT
Cuts at: 5 52 4282 4822 5578 5
Size: 47 4230 540 756 2081
Fragments arranged by size:
4230 2081 756 540 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7654
StyI C'CwwG_G
Cuts at: 513 1065 2004 3678 5093 513
Size: 552 939 1674 1415 3074
Fragments arranged by size:
3074 1674 1415 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2881 3133 3430 3845 5681 5698 5851
Size: 1255 1301 252 297 415 1836 17 153
Cuts at: 5851 6036 6481 325
Size: 185 445 1498
Fragments arranged by size:
1836 1498 1301 1255 445 415 297 252
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3941 5900 7612
Size: 274 80 33 51 1443 1964 1959 1712
Cuts at: 7612 96
Size: 138
Fragments arranged by size:
1964 1959 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3108 3140 7614 7646 3108
Size: 32 4474 32 3116
Fragments arranged by size:
4474 3116 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4326 1316
Size: 3010 4644
XbaI T'CTAG_A
Cuts at: 2366 4990 2366
Size: 2624 5030
XhoI C'TCGA_G
Cuts at: 1943 2066 1943
Size: 123 7531
XmaI C'CCGG_G
Cuts at: 1870 4979 1870
Size: 3109 4545
XmnI GAAnn'nnTTC
Cuts at: 2295 2491 4510 4647 4866 5783 2295
Size: 196 2019 137 219 917 4166
Fragments arranged by size:
4166 2019 917 219 196 137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5462 276
Size: 53 83 186 936 3928 2468
Fragments arranged by size:
3928 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI