Restriction map for
pFN21AE5761
(Circular) (Six-base) MAPSORT of: pFN21AE5761.seq Check: 1856 from: 1 to: 7138
pFN21AE5761 7138 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4948 278
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4481 1937
Size: 2544 4594
Acc65I G'GTAC_C
Cuts at: 4459 4459
Size: 7138
AclI AA'CG_TT
Cuts at: 1563 5265 5638 1563
Size: 3702 373 3063
Fragments arranged by size:
3702 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2208 2273 2861 2960 5201 6585 6853
Size: 446 130 65 588 99 2241 1384 268
Cuts at: 6853 1632
Size: 1917
Fragments arranged by size:
2241 1917 1384 588 446 268 130 99
65
AfeI AGC'GCT
Cuts at: 4193 4193
Size: 7138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6941
AflIII A'CryG_T
Cuts at: 1203 6037 7014 1203
Size: 4834 977 1327
Fragments arranged by size:
4834 1327 977
AgeI A'CCGG_T
Cuts at: 5969 5969
Size: 7138
AhdI GACnn_n'nnGTC
Cuts at: 5867 5867
Size: 7138
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7138
AlwNI CAG_nnn'CTG
Cuts at: 2513 3635 4217 4235 4881 6453 2513
Size: 1122 582 18 646 1572 3198
Fragments arranged by size:
3198 1572 1122 646 582 18
ApaLI G'TGCA_C
Cuts at: 5195 6351 5195
Size: 1156 5982
ApoI r'AATT_y
Cuts at: 1493 2872 3514 3748 4447 4673 4825 6934 1493
Size: 1379 642 234 699 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 699 642 234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5692 160
Size: 872 4660 1606
Fragments arranged by size:
4660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
AvaI C'yCGr_G
Cuts at: 1870 1943 2545 2650 3198 4463 1870
Size: 73 602 105 548 1265 4545
Fragments arranged by size:
4545 1265 602 548 105 73
BamHI G'GATC_C
Cuts at: 4468 4835 4468
Size: 367 6771
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2461 4459 5914 618
Size: 325 217 627 674 1998 1455 1842
Fragments arranged by size:
1998 1842 1455 674 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 3314 4457 1391
Size: 846 1077 1143 4072
Fragments arranged by size:
4072 1143 1077 846
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2688 3768 928
Size: 971 789 1080 4298
Fragments arranged by size:
4298 1080 971 789
BbvCI CC'TCA_GC
Cuts at: 3208 3208
Size: 7138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5329 5363 1903
Size: 34 3392 34 3678
Fragments arranged by size:
3678 3392 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2260 3661 5030 6240 2260
Size: 1401 1369 1210 3158
Fragments arranged by size:
3158 1401 1369 1210
BclI T'GATC_A
Cuts at: 1548 2377 1548
Size: 829 6309
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4278 5749 136
Size: 107 122 71 631 605 2606 1471 1525
Fragments arranged by size:
2606 1525 1471 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7133 7133
Size: 7138
BlpI GC'TnA_GC
Cuts at: 4555 4555
Size: 7138
Bme1580I G_kGCm'C
Cuts at: 1790 4269 5199 6355 1790
Size: 2479 930 1156 2573
Fragments arranged by size:
2573 2479 1156 930
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3161 3711 3855
Size: 416 53 83 399 24 1744 550 144
Cuts at: 3855 3869 4869 5827 442
Size: 14 1000 958 1753
Fragments arranged by size:
1753 1744 1000 958 550 416 399 144
83 53 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 3362 4194 4325 5798 1239
Size: 1400 723 832 131 1473 2579
Fragments arranged by size:
2579 1473 1400 832 723 131
Bpu10I CC'TnA_GC
Cuts at: 2409 3208 2409
Size: 799 6339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 4078 5263 6128 6426 2932
Size: 1146 1185 865 298 3644
Fragments arranged by size:
3644 1185 1146 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 3082 5801 882
Size: 617 1401 182 2719 2219
Fragments arranged by size:
2719 2219 1401 617 182
BsaAI yAC'GTr
Cuts at: 493 1204 4929 493
Size: 711 3725 2702
Fragments arranged by size:
3725 2702 711
BsaBI GATnn'nnATC
Cuts at: 7132 7132
Size: 7138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4945 5327 5962 275
Size: 53 83 186 936 3412 382 635 1451
Fragments arranged by size:
3412 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5571 5969 6243 6390 6801 5571
Size: 398 274 147 411 5908
Fragments arranged by size:
5908 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3879 3909 4280 4310 1178
Size: 30 165 30 2476 30 371 30 4006
Fragments arranged by size:
4006 2476 371 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2337 2837 3162 3638 4339 1186
Size: 1151 500 325 476 701 3985
Fragments arranged by size:
3985 1151 701 500 476 325
BseYI C'CCAG_C
Cuts at: 2218 3324 4268 4299 4316 6341 6837 2218
Size: 1106 944 31 17 2025 496 2519
Fragments arranged by size:
2519 2025 1106 944 496 31 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6565
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4588 5349 6377 1540
Size: 275 186 1726 861 761 1028 2301
Fragments arranged by size:
2301 1726 1028 861 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2984 3166 5199 5284 6355 2237
Size: 747 182 2033 85 1071 3020
Fragments arranged by size:
3020 2033 1071 747 182 85
BsmI GAATG_Cn'
Cuts at: 4649 4742 4649
Size: 93 7045
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2984 3166 3314 4269 4457 5199
Size: 399 447 747 182 148 955 188 742
Cuts at: 5199 5284 6355 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 955 747 742 447 399 188
182 148 85
BspEI T'CCGG_A
Cuts at: 6801 6801
Size: 7138
BspHI T'CATG_A
Cuts at: 5027 6757 5027
Size: 1730 5408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 2825 3100 4123 4493 844
Size: 1759 222 275 1023 370 3489
Fragments arranged by size:
3489 1759 1023 370 275 222
BsrBI CCG'CTC
Cuts at: 1394 1823 5025 1394
Size: 429 3202 3507
Fragments arranged by size:
3507 3202 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 4176 5633 5807 65
Size: 1183 1075 1853 1457 174 1396
Fragments arranged by size:
1853 1457 1396 1183 1075 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2274 4272 5782 5969 1700
Size: 110 464 1998 1510 187 2869
Fragments arranged by size:
2869 1998 1510 464 187 110
BsrGI T'GTAC_A
Cuts at: 96 3394 96
Size: 3298 3840
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7138
BssSI C'ACGA_G
Cuts at: 5198 6210 5198
Size: 1012 6126
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4384 794
Size: 3590 3548
BstBI TT'CG_AA
Cuts at: 5942 5942
Size: 7138
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7138
BstYI r'GATC_y
Cuts at: 1972 3280 4468 4835 5220 5237 6689 6775 6787
Size: 1308 1188 367 385 17 1452 86 12
Cuts at: 6787 7133 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1308 1188 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5647
Fragments arranged by size:
5647 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2072 4295 487
Size: 683 56 846 2223 3330
Fragments arranged by size:
3330 2223 846 683 56
BtsI GCAGTG_nn'
Cuts at: 4654 5448 5468 4654
Size: 794 20 6324
Fragments arranged by size:
6324 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7103
DraI TTT'AAA
Cuts at: 4442 4442
Size: 7138
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2613 6145 809
Size: 507 1297 3532 1802
Fragments arranged by size:
3532 1802 1297 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2328 4585 5474
Size: 54 1425 50 275 18 498 2257 889
Cuts at: 5474 7103 8
Size: 1629 43
Fragments arranged by size:
2257 1629 1425 889 498 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4585 1537
Size: 275 2773 4090
Fragments arranged by size:
4090 2773 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2589 2754 2898 3526 3739 5068 1353
Size: 975 261 165 144 628 213 1329 3423
Fragments arranged by size:
3423 1329 975 628 261 213 165 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2245 3887 5725 6099 6245 387
Size: 1064 138 656 1642 1838 374 146 1280
Fragments arranged by size:
1838 1642 1280 1064 656 374 146 138
EcoICRI GAG'CTC
Cuts at: 2235 2235
Size: 7138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2208 2273 2639 2861 2960 3362
Size: 393 446 130 65 366 222 99 402
Cuts at: 3362 4194 4325 5201 5798 6585 6853 1239
Size: 832 131 876 597 787 268 1524
Fragments arranged by size:
1524 876 832 787 597 446 402 393
366 268 222 131 130 99 65
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2456 2360
Size: 96 7042
EcoO109I rG'GnC_Cy
Cuts at: 2188 2188
Size: 7138
EcoRI G'AATT_C
Cuts at: 2872 3514 4447 4825 2872
Size: 642 933 378 5185
Fragments arranged by size:
5185 933 642 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2798 3665 3935 2798
Size: 867 270 6001
Fragments arranged by size:
6001 867 270
FspI TGC'GCA
Cuts at: 2291 3299 5644 2291
Size: 1008 2345 3785
Fragments arranged by size:
3785 2345 1008
HaeII r_GCGC'y
Cuts at: 1678 4195 6285 1678
Size: 2517 2090 2531
Fragments arranged by size:
2531 2517 2090
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3617 3649 3716 3748 4240
Size: 32 135 32 2045 32 67 32 492
Cuts at: 4240 4272 5779 5811 5853 5885 1373
Size: 32 1507 32 42 32 2626
Fragments arranged by size:
2626 2045 1507 492 135 67 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2583 2857 3077 4482 4728 669
Size: 988 281 645 274 220 1405 246 3079
Fragments arranged by size:
3079 1405 988 645 281 274 246 220
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7138
HpaI GTT'AAC
Cuts at: 4728 4728
Size: 7138
KpnI G_GTAC'C
Cuts at: 4463 4463
Size: 7138
MfeI C'AATT_G
Cuts at: 3243 4737 3243
Size: 1494 5644
MluI A'CGCG_T
Cuts at: 7014 7014
Size: 7138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 6252 6436 1305
Size: 939 4008 184 2007
Fragments arranged by size:
4008 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5316
Fragments arranged by size:
5316 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3551 5096 5455 5614 518
Size: 648 2385 1545 359 159 2042
Fragments arranged by size:
2385 2042 1545 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3212 3340 4235 4303 4554 5231 6379 6624
Size: 1606 128 895 68 251 677 1148 245
Cuts at: 6624 1606
Size: 2120
Fragments arranged by size:
2120 1606 1148 895 677 251 245 128
68
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5647
Fragments arranged by size:
5647 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7138
NotI GC'GGCC_GC
Cuts at: 4585 4585
Size: 7138
NspI r_CATG'y
Cuts at: 1132 1229 2207 3298 3556 4294 4496 6041 1132
Size: 97 978 1091 258 738 202 1545 2229
Fragments arranged by size:
2229 1545 1091 978 738 258 202 97
PasI CC'CwG_GG
Cuts at: 1348 3965 3991 1348
Size: 2617 26 4495
Fragments arranged by size:
4495 2617 26
PciI A'CATG_T
Cuts at: 6037 6037
Size: 7138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3635 4217 1108
Size: 2527 582 4029
Fragments arranged by size:
4029 2527 582
PfoI T'CCnGG_A
Cuts at: 2638 4147 2638
Size: 1509 5629
PmeI GTTT'AAAC
Cuts at: 4442 4442
Size: 7138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5207 5239 6720 6752 5207
Size: 32 1481 32 5593
Fragments arranged by size:
5593 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2188 2188
Size: 7138
PshAI GACnn'nnGTC
Cuts at: 1613 4059 1613
Size: 2446 4692
PsiI TTA'TAA
Cuts at: 4708 4708
Size: 7138
PspXI vC'TCGA_Gb
Cuts at: 3198 3198
Size: 7138
PstI C_TGCA'G
Cuts at: 830 3063 4490 830
Size: 2233 1427 3478
Fragments arranged by size:
3478 2233 1427
PvuI CG_AT'CG
Cuts at: 2001 3727 2001
Size: 1726 5412
PvuII CAG'CTG
Cuts at: 3212 3340 4235 4303 3212
Size: 128 895 68 6047
Fragments arranged by size:
6047 895 128 68
SacI G_AGCT'C
Cuts at: 2237 2237
Size: 7138
SalI G'TCGA_C
Cuts at: 1936 4480 1936
Size: 2544 4594
SanDI GG'GwC_CC
Cuts at: 2188 2188
Size: 7138
SapI GCTCTTCn'nnn_
Cuts at: 2589 2589
Size: 7138
SbfI CC_TGCA'GG
Cuts at: 3063 4490 3063
Size: 1427 5711
ScaI AGT'ACT
Cuts at: 5386 7098 5386
Size: 1712 5426
SfcI C'TryA_G
Cuts at: 826 1045 2720 3059 4187 4373 4486 5621 6302
Size: 219 1675 339 1128 186 113 1135 681
Cuts at: 6302 6493 826
Size: 191 1471
Fragments arranged by size:
1675 1471 1135 1128 681 339 219 191
186 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7138
SmaI CCC'GGG
Cuts at: 1872 4465 1872
Size: 2593 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2911 3198 4057 5242 6143 6405
Size: 197 926 968 287 859 1185 901 262
Cuts at: 6405 820
Size: 1553
Fragments arranged by size:
1553 1185 968 926 901 859 287 262
197
SnaBI TAC'GTA
Cuts at: 493 4929 493
Size: 4436 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7138
SphI G_CATG'C
Cuts at: 1132 2207 3298 3556 4496 1132
Size: 1075 1091 258 940 3774
Fragments arranged by size:
3774 1091 1075 940 258
SspI AAT'ATT
Cuts at: 5 52 5062 5
Size: 47 5010 2081
Fragments arranged by size:
5010 2081 47
StuI AGG'CCT
Cuts at: 1517 2146 3946 4281 1517
Size: 629 1800 335 4374
Fragments arranged by size:
4374 1800 629 335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2390 3289 3864 4577 513
Size: 552 939 386 899 575 713 3074
Fragments arranged by size:
3074 939 899 713 575 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 3874 3978 5165 5182 5335 5520
Size: 1255 432 1862 104 1187 17 153 185
Cuts at: 5520 5965 325
Size: 445 1498
Fragments arranged by size:
1862 1498 1255 1187 445 432 185 153
104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3394 3997 5384
Size: 274 80 33 51 1443 1417 603 1387
Cuts at: 5384 7096 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1417 1387 603 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3879 3911 4280 4312 7098 7130 3879
Size: 32 369 32 2786 32 3887
Fragments arranged by size:
3887 2786 369 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3487 1316
Size: 2171 4967
XbaI T'CTAG_A
Cuts at: 4474 4474
Size: 7138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3422 3422
Size: 7138
XhoI C'TCGA_G
Cuts at: 1943 3198 1943
Size: 1255 5883
XmaI C'CCGG_G
Cuts at: 1870 4463 1870
Size: 2593 4545
XmnI GAAnn'nnTTC
Cuts at: 5267 5267
Size: 7138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4946 276
Size: 53 83 186 936 3412 2468
Fragments arranged by size:
3412 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI