Restriction map for
pFN21AE5758
(Circular) (Six-base) MAPSORT of: pFN21AE5758.seq Check: 2213 from: 1 to: 7042
pFN21AE5758 7042 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4852 278
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4385 1937
Size: 2448 4594
Acc65I G'GTAC_C
Cuts at: 2705 4363 2705
Size: 1658 5384
AclI AA'CG_TT
Cuts at: 1563 5169 5542 1563
Size: 3606 373 3063
Fragments arranged by size:
3606 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2158 4181 4241 5105 6489 6757 1632
Size: 526 2023 60 864 1384 268 1917
Fragments arranged by size:
2023 1917 1384 864 526 268 60
AfeI AGC'GCT
Cuts at: 2348 3527 2348
Size: 1179 5863
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 5941 6918 1203
Size: 4738 977 1327
Fragments arranged by size:
4738 1327 977
AgeI A'CCGG_T
Cuts at: 5873 5873
Size: 7042
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 4271 1166
Size: 3105 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7042
AlwNI CAG_nnn'CTG
Cuts at: 4106 4785 6357 4106
Size: 679 1572 4791
Fragments arranged by size:
4791 1572 679
ApaLI G'TGCA_C
Cuts at: 5099 6255 5099
Size: 1156 5886
ApoI r'AATT_y
Cuts at: 1493 3412 3654 4199 4351 4577 4729 6838 1493
Size: 1919 242 545 152 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 545 242 226 152 152
AseI AT'TA_AT
Cuts at: 160 1032 2514 3284 3944 5596 160
Size: 872 1482 770 660 1652 1606
Fragments arranged by size:
1652 1606 1482 872 770 660
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 4367 1870
Size: 73 2424 4545
Fragments arranged by size:
4545 2424 73
BamHI G'GATC_C
Cuts at: 2393 4372 4739 2393
Size: 1979 367 4696
Fragments arranged by size:
4696 1979 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2705 3223 4363 5818
Size: 325 217 627 369 549 518 1140 1455
Cuts at: 5818 618
Size: 1842
Fragments arranged by size:
1842 1455 1140 627 549 518 369 325
217
BanII G_rGCy'C
Cuts at: 1391 3576 4361 1391
Size: 2185 785 4072
Fragments arranged by size:
4072 2185 785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 4321 928
Size: 971 1474 948 3649
Fragments arranged by size:
3649 1474 971 948
BbvCI CC'TCA_GC
Cuts at: 3542 3542
Size: 7042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5233 5267 1903
Size: 34 3296 34 3678
Fragments arranged by size:
3678 3296 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4934 6144 4934
Size: 1210 5832
BclI T'GATC_A
Cuts at: 1548 3088 3916 1548
Size: 1540 828 4674
Fragments arranged by size:
4674 1540 828
BfrBI ATG'CAT
Cuts at: 3239 3420 3239
Size: 181 6861
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5653 136
Size: 107 122 71 631 605 3981 1525
Fragments arranged by size:
3981 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 7037 2085
Size: 4952 2090
BlpI GC'TnA_GC
Cuts at: 4459 4459
Size: 7042
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2159 5103 6259 1790
Size: 350 19 2944 1156 2573
Fragments arranged by size:
2944 2573 1156 350 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7042
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4773 5731 442
Size: 416 53 83 399 24 3356 958 1753
Fragments arranged by size:
3356 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 3609 4155 5702 1239
Size: 1676 694 546 1547 2579
Fragments arranged by size:
2579 1676 1547 694 546
Bpu10I CC'TnA_GC
Cuts at: 3542 3542
Size: 7042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5167 6032 6330 5167
Size: 865 298 5879
Fragments arranged by size:
5879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5705 882
Size: 617 4206 2219
Fragments arranged by size:
4206 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4833 493
Size: 711 3629 2702
Fragments arranged by size:
3629 2702 711
BsaBI GATnn'nnATC
Cuts at: 7036 7036
Size: 7042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 4849 5231 5866
Size: 53 83 186 936 825 2491 382 635
Cuts at: 5866 275
Size: 1451
Fragments arranged by size:
2491 1451 936 825 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5475 5873 6147 6294 6705 5475
Size: 398 274 147 411 5812
Fragments arranged by size:
5812 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 2946 2976 3436
Size: 30 165 30 1468 30 45 30 460
Cuts at: 3436 3466 4041 4071 1178
Size: 30 575 30 4149
Fragments arranged by size:
4149 1468 575 460 165 45 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2970 3875 1186
Size: 1204 580 905 4353
Fragments arranged by size:
4353 1204 905 580
BseYI C'CCAG_C
Cuts at: 2281 6245 6741 2281
Size: 3964 496 2582
Fragments arranged by size:
3964 2582 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6469
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2632 3576 5103 5188 6259 2632
Size: 944 1527 85 1071 3415
Fragments arranged by size:
3415 1527 1071 944 85
BsmI GAATG_Cn'
Cuts at: 2497 4553 4646 2497
Size: 2056 93 4893
Fragments arranged by size:
4893 2056 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2793 2963 2793
Size: 170 6872
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2159 2632 3576 4361 5103 5188
Size: 399 350 19 473 944 785 742 85
Cuts at: 5188 6259 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 785 742 473 399 350
85 19
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 3846 4931 6661 3846
Size: 1085 1730 4227
Fragments arranged by size:
4227 1730 1085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4397 844
Size: 3553 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4929 1394
Size: 429 3106 3507
Fragments arranged by size:
3507 3106 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5537 5711 65
Size: 1183 4289 174 1396
Fragments arranged by size:
4289 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5686 5873 1700
Size: 110 3876 187 2869
Fragments arranged by size:
3876 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7042
BssSI C'ACGA_G
Cuts at: 2853 4080 5102 6114 2853
Size: 1227 1022 1012 3781
Fragments arranged by size:
3781 1227 1022 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7042
BstBI TT'CG_AA
Cuts at: 5846 5846
Size: 7042
BstEII G'GTnAC_C
Cuts at: 1184 4183 4192 1184
Size: 2999 9 4034
Fragments arranged by size:
4034 2999 9
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 7042
BstYI r'GATC_y
Cuts at: 1972 2085 2393 3160 4307 4372 4739 5124 5141
Size: 113 308 767 1147 65 367 385 17
Cuts at: 5141 6593 6679 6691 7037 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1147 767 385 367 346 308
113 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2641 513
Size: 552 1576 4914
Fragments arranged by size:
4914 1576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6303
Fragments arranged by size:
6303 683 56
BtsI GCAGTG_nn'
Cuts at: 4558 5352 5372 4558
Size: 794 20 6228
Fragments arranged by size:
6228 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7007
DraI TTT'AAA
Cuts at: 3759 4346 3759
Size: 587 6455
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 7042
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6049 809
Size: 507 4733 1802
Fragments arranged by size:
4733 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4489 5378 7007
Size: 54 1425 50 275 18 2659 889 1629
Cuts at: 7007 8
Size: 43
Fragments arranged by size:
2659 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2791 3684 4173 4972 1353
Size: 1438 893 489 799 3423
Fragments arranged by size:
3423 1438 893 799 489
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5629 6003 6149 387
Size: 1064 138 4040 374 146 1280
Fragments arranged by size:
4040 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3574 3574
Size: 7042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2915 3609 4155 4181 4241 5105
Size: 393 526 757 694 546 26 60 864
Cuts at: 5105 5702 6489 6757 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 864 787 757 694 597 546 526
393 268 60 26
EcoO109I rG'GnC_Cy
Cuts at: 2788 3094 4103 2788
Size: 306 1009 5727
Fragments arranged by size:
5727 1009 306
EcoRI G'AATT_C
Cuts at: 4351 4729 4351
Size: 378 6664
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 7042
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2413 2759 3338 2188
Size: 225 346 579 5892
Fragments arranged by size:
5892 579 346 225
FspI TGC'GCA
Cuts at: 5548 5548
Size: 7042
HaeII r_GCGC'y
Cuts at: 1678 2350 3274 3529 6189 1678
Size: 672 924 255 2660 2531
Fragments arranged by size:
2660 2531 924 672 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2732 2764 2946
Size: 32 135 32 505 32 623 32 182
Cuts at: 2946 2978 5683 5715 5757 5789 1373
Size: 32 2705 32 42 32 2626
Fragments arranged by size:
2705 2626 623 505 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2529 4386 4632 669
Size: 988 281 115 476 1857 246 3079
Fragments arranged by size:
3079 1857 988 476 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7042
HpaI GTT'AAC
Cuts at: 2053 4632 2053
Size: 2579 4463
KpnI G_GTAC'C
Cuts at: 2709 4367 2709
Size: 1658 5384
MfeI C'AATT_G
Cuts at: 2988 4641 2988
Size: 1653 5389
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3889 6156 6340 1305
Size: 2584 2267 184 2007
Fragments arranged by size:
2584 2267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5220
Fragments arranged by size:
5220 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2307 4271 5000 5359 5518 518
Size: 648 1141 1964 729 359 159 2042
Fragments arranged by size:
2042 1964 1141 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 3398 4458 5135 6283 6528 1606
Size: 1156 636 1060 677 1148 245 2120
Fragments arranged by size:
2120 1156 1148 1060 677 636 245
NcoI C'CATG_G
Cuts at: 513 1065 2641 513
Size: 552 1576 4914
Fragments arranged by size:
4914 1576 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7042
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NsiI A_TGCA'T
Cuts at: 3241 3422 3241
Size: 181 6861
NspI r_CATG'y
Cuts at: 1132 1229 4400 5945 1132
Size: 97 3171 1545 2229
Fragments arranged by size:
3171 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 5941 5941
Size: 7042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7042
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3094 4103 3094
Size: 1009 6033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7042
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PstI C_TGCA'G
Cuts at: 830 3294 3562 4394 830
Size: 2464 268 832 3478
Fragments arranged by size:
3478 2464 832 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
PvuII CAG'CTG
Cuts at: 2762 3398 2762
Size: 636 6406
SacI G_AGCT'C
Cuts at: 3576 3576
Size: 7042
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SapI GCTCTTCn'nnn_
Cuts at: 3684 3684
Size: 7042
SbfI CC_TGCA'GG
Cuts at: 4394 4394
Size: 7042
ScaI AGT'ACT
Cuts at: 5290 7002 5290
Size: 1712 5330
SexAI A'CCwGG_T
Cuts at: 3739 3739
Size: 7042
SfcI C'TryA_G
Cuts at: 826 1045 3290 3558 4390 5525 6206 6397 826
Size: 219 2245 268 832 1135 681 191 1471
Fragments arranged by size:
2245 1471 1135 832 681 268 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 4369 1872
Size: 2497 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5146 6047 6309 820
Size: 197 926 3203 901 262 1553
Fragments arranged by size:
3203 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4833 493
Size: 4340 2702
SpeI A'CTAG_T
Cuts at: 152 3638 152
Size: 3486 3556
SphI G_CATG'C
Cuts at: 1132 4400 1132
Size: 3268 3774
SspI AAT'ATT
Cuts at: 5 52 4966 5
Size: 47 4914 2081
Fragments arranged by size:
4914 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7042
StyI C'CwwG_G
Cuts at: 513 1065 2119 2532 2641 2791 4188 4481 513
Size: 552 1054 413 109 150 1397 293 3074
Fragments arranged by size:
3074 1397 1054 552 413 293 150 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5069 5086 5239 5424 5869 325
Size: 1255 3489 17 153 185 445 1498
Fragments arranged by size:
3489 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5288 7000 96
Size: 274 80 33 51 1443 3311 1712 138
Fragments arranged by size:
3311 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2871 2876 2903 3436 3468 4041 4073 7002
Size: 27 5 27 533 32 573 32 2929
Cuts at: 7002 7034 2844
Size: 32 2852
Fragments arranged by size:
2929 2852 573 533 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7042
XbaI T'CTAG_A
Cuts at: 4310 4378 4310
Size: 68 6974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2603 2603
Size: 7042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 4367 1870
Size: 2497 4545
XmnI GAAnn'nnTTC
Cuts at: 2171 5171 2171
Size: 3000 4042
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4850 276
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI