Restriction map for
pFN21AE5754
(Circular) (Six-base) MAPSORT of: pFN21AE5754.seq Check: 46 from: 1 to: 6913
pFN21AE5754 6913 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4723 278
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4256 1937
Size: 2319 4594
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2882 4056 4976 6360 6628 1632
Size: 1250 1174 920 1384 268 1917
Fragments arranged by size:
1917 1384 1250 1174 920 268
AflII C'TTAA_G
Cuts at: 820 1017 2437 820
Size: 197 1420 5296
Fragments arranged by size:
5296 1420 197
AflIII A'CryG_T
Cuts at: 1203 2368 5812 6789 1203
Size: 1165 3444 977 1327
Fragments arranged by size:
3444 1327 1165 977
AgeI A'CCGG_T
Cuts at: 5744 5744
Size: 6913
AhdI GACnn_n'nnGTC
Cuts at: 3842 5642 3842
Size: 1800 5113
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2785 1184
Size: 1601 5312
AlwNI CAG_nnn'CTG
Cuts at: 4656 6228 4656
Size: 1572 5341
ApaLI G'TGCA_C
Cuts at: 4970 6126 4970
Size: 1156 5757
ApoI r'AATT_y
Cuts at: 1493 2289 2474 2480 2628 2887 2971 3020 3051
Size: 796 185 6 148 259 84 49 31
Cuts at: 3051 3097 3227 3501 4186 4222 4448 4600 6709
Size: 46 130 274 685 36 226 152 2109
Cuts at: 6709 1493
Size: 1697
Fragments arranged by size:
2109 1697 796 685 274 259 226 185
152 148 130 84 49 46 36 31
6
AseI AT'TA_AT
Cuts at: 160 1032 2126 5467 160
Size: 872 1094 3341 1606
Fragments arranged by size:
3341 1606 1094 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3550 4238 1870
Size: 73 964 643 688 4545
Fragments arranged by size:
4545 964 688 643 73
BamHI G'GATC_C
Cuts at: 2303 2902 4243 4610 2303
Size: 599 1341 367 4606
Fragments arranged by size:
4606 1341 599 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4234 5689 618
Size: 325 217 627 2447 1455 1842
Fragments arranged by size:
2447 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4232 1391
Size: 2841 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5942
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5104 5138 1903
Size: 34 3167 34 3678
Fragments arranged by size:
3678 3167 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2790 4805 6015 2790
Size: 2015 1210 3688
Fragments arranged by size:
3688 2015 1210
BclI T'GATC_A
Cuts at: 1548 2104 1548
Size: 556 6357
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5524 136
Size: 107 122 71 631 605 3852 1525
Fragments arranged by size:
3852 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2378 2548 6908 2378
Size: 170 4360 2383
Fragments arranged by size:
4360 2383 170
BlpI GC'TnA_GC
Cuts at: 3693 4330 3693
Size: 637 6276
Bme1580I G_kGCm'C
Cuts at: 1790 4974 6130 1790
Size: 3184 1156 2573
Fragments arranged by size:
3184 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2702 1318
Size: 1384 5529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3250 4119 4644
Size: 416 53 83 399 24 1833 869 525
Cuts at: 4644 5602 442
Size: 958 1753
Fragments arranged by size:
1833 1753 958 869 525 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3589 3624 4137 5573 1239
Size: 2350 35 513 1436 2579
Fragments arranged by size:
2579 2350 1436 513 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3107 5038 5903 6201 3107
Size: 1931 865 298 3819
Fragments arranged by size:
3819 1931 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5576 882
Size: 617 4077 2219
Fragments arranged by size:
4077 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2979 3301 4704 493
Size: 711 1775 322 1403 2702
Fragments arranged by size:
2702 1775 1403 711 322
BsaBI GATnn'nnATC
Cuts at: 2928 3540 6907 2928
Size: 612 3367 2934
Fragments arranged by size:
3367 2934 612
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 4720 5102 5737
Size: 53 83 186 936 600 2587 382 635
Cuts at: 5737 275
Size: 1451
Fragments arranged by size:
2587 1451 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5346 5744 6018 6165 6576 5346
Size: 398 274 147 411 5683
Fragments arranged by size:
5683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6688
Fragments arranged by size:
6688 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4086 1186
Size: 2900 4013
BseYI C'CCAG_C
Cuts at: 6116 6612 6116
Size: 496 6417
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4363 5124 6152 1540
Size: 275 186 2362 761 1028 2301
Fragments arranged by size:
2362 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4974 5059 6130 4974
Size: 85 1071 5757
Fragments arranged by size:
5757 1071 85
BsmI GAATG_Cn'
Cuts at: 4424 4517 4424
Size: 93 6820
BsmBI CGTCTCn'nnnn_
Cuts at: 2550 2550
Size: 6913
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4232 4974 5059 6130 1391
Size: 399 2442 742 85 1071 2174
Fragments arranged by size:
2442 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 4802 6532 4802
Size: 1730 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4268 844
Size: 3424 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4800 1394
Size: 429 2977 3507
Fragments arranged by size:
3507 2977 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2757 5408 5582 65
Size: 1183 1509 2651 174 1396
Fragments arranged by size:
2651 1509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5557 5744 1700
Size: 110 3747 187 2869
Fragments arranged by size:
3747 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3402 96
Size: 3306 3607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 4973 5985 4973
Size: 1012 5901
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2641 794
Size: 1847 5066
BstBI TT'CG_AA
Cuts at: 5717 5717
Size: 6913
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6913
BstYI r'GATC_y
Cuts at: 1972 2303 2378 2548 2902 3938 4243 4610 4995
Size: 331 75 170 354 1036 305 367 385
Cuts at: 4995 5012 6464 6550 6562 6908 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1036 385 367 354 346 331
305 170 86 75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6174
Fragments arranged by size:
6174 683 56
BtsI GCAGTG_nn'
Cuts at: 4029 4429 5223 5243 4029
Size: 400 794 20 5699
Fragments arranged by size:
5699 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2352 2387 529
Size: 35 1788 35 5055
Fragments arranged by size:
5055 1788 35 35
DraI TTT'AAA
Cuts at: 3025 4217 3025
Size: 1192 5721
DraIII CAC_nnn'GTG
Cuts at: 2354 3728 3813 2354
Size: 1374 85 5454
Fragments arranged by size:
5454 1374 85
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5920 809
Size: 507 4604 1802
Fragments arranged by size:
4604 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2843 4360 5249
Size: 54 1425 50 275 18 1013 1517 889
Cuts at: 5249 6878 8
Size: 1629 43
Fragments arranged by size:
1629 1517 1425 1013 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4360 1537
Size: 275 2548 4090
Fragments arranged by size:
4090 2548 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4843 1353
Size: 3490 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3570 5500 5874 6020 387
Size: 1064 138 1981 1930 374 146 1280
Fragments arranged by size:
1981 1930 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2882 3589 3624 4056 4137 4976 5573
Size: 393 1250 707 35 432 81 839 597
Cuts at: 5573 6360 6628 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1250 839 787 707 597 432 393
268 81 35
EcoNI CCTnn'n_nnAGG
Cuts at: 3666 3666
Size: 6913
EcoO109I rG'GnC_Cy
Cuts at: 4021 4021
Size: 6913
EcoRI G'AATT_C
Cuts at: 2971 4222 4600 2971
Size: 1251 378 5284
Fragments arranged by size:
5284 1251 378
FspI TGC'GCA
Cuts at: 5419 5419
Size: 6913
HaeII r_GCGC'y
Cuts at: 1678 6060 1678
Size: 4382 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3368 3400 3828 3860 4133
Size: 32 135 32 1796 32 428 32 273
Cuts at: 4133 4165 4192 4224 5554 5586 5628 5660 1373
Size: 32 27 32 1330 32 42 32 2626
Fragments arranged by size:
2626 1796 1330 428 273 135 42 32
32 32 32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 2048 3358 3778 4257 4503 669
Size: 988 281 110 1310 420 479 246 3079
Fragments arranged by size:
3079 1310 988 479 420 281 246 110
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6913
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 2774 4512 2774
Size: 1738 5175
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 3154 3187 4115 6027 6211 1305
Size: 1434 415 33 928 1912 184 2007
Fragments arranged by size:
2007 1912 1434 928 415 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2845 10
Size: 54 1425 343 1013 4078
Fragments arranged by size:
4078 1425 1013 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 3758 3897 4871 5230 5389 518
Size: 648 1255 1337 139 974 359 159 2042
Fragments arranged by size:
2042 1337 1255 974 648 359 159 139
MspA1I CmG'CkG
Cuts at: 1606 4329 5006 6154 6399 1606
Size: 2723 677 1148 245 2120
Fragments arranged by size:
2723 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6361
NdeI CA'TA_TG
Cuts at: 387 3373 387
Size: 2986 3927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NspI r_CATG'y
Cuts at: 1132 1229 2372 2722 4271 5816 1132
Size: 97 1143 350 1549 1545 2229
Fragments arranged by size:
2229 1549 1545 1143 350 97
PasI CC'CwG_GG
Cuts at: 1348 3266 3880 1348
Size: 1918 614 4381
Fragments arranged by size:
4381 1918 614
PciI A'CATG_T
Cuts at: 2368 5812 2368
Size: 3444 3469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 3764 1108
Size: 1077 1579 4257
Fragments arranged by size:
4257 1579 1077
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4021 4021
Size: 6913
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 2471 4483 2471
Size: 2012 4901
PstI C_TGCA'G
Cuts at: 830 3499 4265 830
Size: 2669 766 3478
Fragments arranged by size:
3478 2669 766
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
SalI G'TCGA_C
Cuts at: 1936 4255 1936
Size: 2319 4594
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 5161 6873 5161
Size: 1712 5201
SfcI C'TryA_G
Cuts at: 826 1045 2308 2445 3495 3666 3783 4261 5396
Size: 219 1263 137 1050 171 117 478 1135
Cuts at: 5396 6077 6268 826
Size: 681 191 1471
Fragments arranged by size:
1471 1263 1135 1050 681 478 219 191
171 137 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2437 3086 5017 5918 6180 820
Size: 197 926 494 649 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 649 494 262 197
SnaBI TAC'GTA
Cuts at: 493 2979 3301 4704 493
Size: 2486 322 1403 2702
Fragments arranged by size:
2702 2486 1403 322
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 4271 1132
Size: 3139 3774
SspI AAT'ATT
Cuts at: 5 52 4837 5
Size: 47 4785 2081
Fragments arranged by size:
4785 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6913
StyI C'CwwG_G
Cuts at: 513 1065 2731 3316 3742 4352 513
Size: 552 1666 585 426 610 3074
Fragments arranged by size:
3074 1666 610 585 552 426
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4940 4957 5110 5295 5740 325
Size: 1255 3360 17 153 185 445 1498
Fragments arranged by size:
3360 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 3199 3402
Size: 274 80 33 51 1443 343 879 203
Cuts at: 3402 5159 6871 96
Size: 1757 1712 138
Fragments arranged by size:
1757 1712 1443 879 343 274 203 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6873 6905 6873
Size: 32 6881
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6913
XbaI T'CTAG_A
Cuts at: 3163 4249 3163
Size: 1086 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 3054 3504 5042 3054
Size: 450 1538 4925
Fragments arranged by size:
4925 1538 450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4721 276
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI