Restriction map for
pFN21AE5747
(Circular) (Six-base) MAPSORT of: pFN21AE5747.seq Check: 6744 from: 1 to: 6619
pFN21AE5747 6619 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2264 4429 278
Size: 53 83 186 936 728 2165 2468
Fragments arranged by size:
2468 2165 936 728 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3516 3962 1937
Size: 1579 446 4594
Fragments arranged by size:
4594 1579 446
Acc65I G'GTAC_C
Cuts at: 2700 3940 2700
Size: 1240 5379
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3016 4682 6066 6334 1632
Size: 1384 1666 1384 268 1917
Fragments arranged by size:
1917 1666 1384 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2670 5518 6495 1203
Size: 1467 2848 977 1327
Fragments arranged by size:
2848 1467 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 3677 1166
Size: 2511 4108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6619
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 6587
AlwNI CAG_nnn'CTG
Cuts at: 2590 3202 3322 3366 3463 3597 4362 5934 2590
Size: 612 120 44 97 134 765 1572 3275
Fragments arranged by size:
3275 1572 765 612 134 120 97 44
ApaI G_GGCC'C
Cuts at: 3894 3894
Size: 6619
ApaLI G'TGCA_C
Cuts at: 2366 3083 4676 5832 2366
Size: 717 1593 1156 3153
Fragments arranged by size:
3153 1593 1156 717
ApoI r'AATT_y
Cuts at: 1493 3928 4154 4306 6415 1493
Size: 2435 226 152 2109 1697
Fragments arranged by size:
2435 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2599 3092 3944 1870
Size: 73 656 493 852 4545
Fragments arranged by size:
4545 852 656 493 73
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 2216 2337 2700 3021
Size: 325 217 627 349 80 121 363 321
Cuts at: 3021 3279 3550 3565 3940 5395 618
Size: 258 271 15 375 1455 1842
Fragments arranged by size:
1842 1455 627 375 363 349 325 321
271 258 217 121 80 15
BanII G_rGCy'C
Cuts at: 1391 2060 2290 2319 2553 3101 3528 3642 3816
Size: 669 230 29 234 548 427 114 174
Cuts at: 3816 3894 3938 1391
Size: 78 44 4072
Fragments arranged by size:
4072 669 548 427 234 230 174 114
78 44 29
BbeI G_GCGC'C
Cuts at: 2220 3554 2220
Size: 1334 5285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5648
BbvCI CC'TCA_GC
Cuts at: 2504 2504
Size: 6619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3647 3681 4810 4844 1903
Size: 34 1710 34 1129 34 3678
Fragments arranged by size:
3678 1710 1129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2092 2601 2772
Size: 107 122 71 631 605 420 509 171
Cuts at: 2772 3105 3743 3820 5230 136
Size: 333 638 77 1410 1525
Fragments arranged by size:
1525 1410 638 631 605 509 420 333
171 122 107 77 71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 2010 2591 2889 4036 2010
Size: 581 298 1147 4593
Fragments arranged by size:
4593 1147 581 298
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2370 3024 3087 3894 4680 5836 1790
Size: 349 231 654 63 807 786 1156 2573
Fragments arranged by size:
2573 1156 807 786 654 349 231 63
BmgBI CAC'GTC
Cuts at: 1318 2420 2673 3798 1318
Size: 1102 253 1125 4139
Fragments arranged by size:
4139 1125 1102 253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3405 4350 5308
Size: 416 53 83 399 24 1988 945 958
Cuts at: 5308 442
Size: 1753
Fragments arranged by size:
1988 1753 958 945 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2540 3235 2540
Size: 695 5924
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2468 3344 3388 3656 3665 5279 1239
Size: 812 417 876 44 268 9 1614 2579
Fragments arranged by size:
2579 1614 876 812 417 268 44 9
Bpu10I CC'TnA_GC
Cuts at: 2504 2504
Size: 6619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 3923 4744 5609 5907 2237
Size: 1686 821 865 298 2949
Fragments arranged by size:
2949 1686 865 821 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3206 3320 5282 882
Size: 617 1707 114 1962 2219
Fragments arranged by size:
2219 1962 1707 617 114
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2084 2217 2261 2877
Size: 53 83 186 936 551 133 44 616
Cuts at: 2877 3551 3746 4426 4808 5443 275
Size: 674 195 680 382 635 1451
Fragments arranged by size:
1451 936 680 674 635 616 551 382
195 186 133 83 53 44
BsaWI w'CCGG_w
Cuts at: 2431 2829 5052 5450 5724 5871 6282 2431
Size: 398 2223 398 274 147 411 2768
Fragments arranged by size:
2768 2223 411 398 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 2468 2498 3143
Size: 30 165 30 874 30 161 30 645
Cuts at: 3143 3173 1178
Size: 30 4624
Fragments arranged by size:
4624 874 645 165 161 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 3502 3560 1186
Size: 1123 1193 58 4245
Fragments arranged by size:
4245 1193 1123 58
BseYI C'CCAG_C
Cuts at: 2311 3815 3882 3887 3894 5822 6318 2311
Size: 1504 67 5 7 1928 496 2612
Fragments arranged by size:
2612 1928 1504 496 67 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2179 3097 1163
Size: 573 443 918 4685
Fragments arranged by size:
4685 918 573 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2426 2871 2998 3691 4069 4830
Size: 275 186 425 445 127 693 378 761
Cuts at: 4830 5858 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 693 445 425 378 275
186 127
BsiHKAI G_wGCw'C
Cuts at: 2016 2370 3087 3528 3642 4680 4765 5836 2016
Size: 354 717 441 114 1038 85 1071 2799
Fragments arranged by size:
2799 1071 1038 717 441 354 114 85
BsmI GAATG_Cn'
Cuts at: 3380 4130 4223 3380
Size: 750 93 5776
Fragments arranged by size:
5776 750 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2365 2365
Size: 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2060 2139 2290 2319 2370 2553
Size: 399 226 44 79 151 29 51 183
Cuts at: 2553 3024 3087 3101 3528 3642 3816 3894 3938
Size: 471 63 14 427 114 174 78 44
Cuts at: 3938 4680 4765 5836 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 427 399 226 183
174 151 114 85 79 78 63 51
44 44 29 14
BspEI T'CCGG_A
Cuts at: 2829 6282 2829
Size: 3453 3166
BspHI T'CATG_A
Cuts at: 4508 6238 4508
Size: 1730 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3224 3974 844
Size: 2380 750 3489
Fragments arranged by size:
3489 2380 750
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 4506 1394
Size: 429 1714 969 3507
Fragments arranged by size:
3507 1714 969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5114 5288 65
Size: 1183 3866 174 1396
Fragments arranged by size:
3866 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2972 3087 3119 3247 3333 3693 5263
Size: 110 1162 115 32 128 86 360 1570
Cuts at: 5263 5450 1700
Size: 187 2869
Fragments arranged by size:
2869 1570 1162 360 187 128 115 110
86 32
BsrGI T'GTAC_A
Cuts at: 96 2490 96
Size: 2394 4225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 2546 4679 5691 2546
Size: 2133 1012 3474
Fragments arranged by size:
3474 2133 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3597 794
Size: 2803 3816
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 2395 1184
Size: 1211 5408
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2227
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 2574 3949 4316 4701 4718 6170 6256 6268
Size: 602 1375 367 385 17 1452 86 12
Cuts at: 6268 6614 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1375 602 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2927 3739 2927
Size: 812 5807
BtgI C'CryG_G
Cuts at: 513 1065 2004 2212 2239 2739 2758 2908 3585
Size: 552 939 208 27 500 19 150 677
Cuts at: 3585 3833 513
Size: 248 3299
Fragments arranged by size:
3299 939 677 552 500 248 208 150
27 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3295 4135 4929 4949 3109
Size: 186 840 794 20 4779
Fragments arranged by size:
4779 840 794 186 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 2403 2716 2403
Size: 313 6306
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2355 2423 2773
Size: 54 1425 50 275 18 525 68 350
Cuts at: 2773 2970 2995 3041 3106 3441 3498 3582 3688
Size: 197 25 46 65 335 57 84 106
Cuts at: 3688 4066 4955 6584 8
Size: 378 889 1629 43
Fragments arranged by size:
1629 1425 889 525 378 350 335 275
197 106 84 68 65 57 54 50
46 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 2423 2995 3688 4066 1537
Size: 275 611 572 693 378 4090
Fragments arranged by size:
4090 693 611 572 378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3870 4549 1353
Size: 2517 679 3423
Fragments arranged by size:
3423 2517 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 2429 2518 3406 5206 5580
Size: 1064 138 624 216 89 888 1800 374
Cuts at: 5580 5726 387
Size: 146 1280
Fragments arranged by size:
1800 1280 1064 888 624 374 216 146
138 89
EcoICRI GAG'CTC
Cuts at: 3526 3640 3526
Size: 114 6505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2468 3016 3344 3388 3656 3665
Size: 393 419 417 548 328 44 268 9
Cuts at: 3665 4682 5279 6066 6334 1239
Size: 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 548 419 417 393
328 268 268 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3166 3314 3738 3166
Size: 148 424 6047
Fragments arranged by size:
6047 424 148
EcoO109I rG'GnC_Cy
Cuts at: 3319 3363 3399 3615 3891 3319
Size: 44 36 216 276 6047
Fragments arranged by size:
6047 276 216 44 36
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
EcoRV GAT'ATC
Cuts at: 2722 2722
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 2220 2896 3554 5766 1678
Size: 542 676 658 2212 2531
Fragments arranged by size:
2531 2212 676 658 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2277 2309 5260 5292 5334
Size: 32 135 32 705 32 2951 32 42
Cuts at: 5334 5366 1373
Size: 32 2626
Fragments arranged by size:
2951 2626 705 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2500 3963 4209 669
Size: 988 281 562 1463 246 3079
Fragments arranged by size:
3079 1463 988 562 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KasI G'GCGC_C
Cuts at: 2216 3550 2216
Size: 1334 5285
KpnI G_GTAC'C
Cuts at: 2704 3944 2704
Size: 1240 5379
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5733 5917 1305
Size: 4428 184 2007
Fragments arranged by size:
4428 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3443 3500 10
Size: 54 1425 343 1611 57 3129
Fragments arranged by size:
3129 1611 1425 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3677 4577 4936 5095 518
Size: 648 2511 900 359 159 2042
Fragments arranged by size:
2511 2042 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2311 2392 2741 2859 3202 3354 3428
Size: 635 70 81 349 118 343 152 74
Cuts at: 3428 3574 3819 4035 4712 5860 6105 1606
Size: 146 245 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 635 349 343 245 245
216 152 146 118 81 74 70
NaeI GCC'GGC
Cuts at: 2974 3121 3249 3695 2974
Size: 147 128 446 5898
Fragments arranged by size:
5898 446 147 128
NarI GG'CG_CC
Cuts at: 2217 3551 2217
Size: 1334 5285
NcoI C'CATG_G
Cuts at: 513 1065 2004 3585 513
Size: 552 939 1581 3547
Fragments arranged by size:
3547 1581 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6619
NgoMIV G'CCGG_C
Cuts at: 2972 3119 3247 3693 2972
Size: 147 128 446 5898
Fragments arranged by size:
5898 446 147 128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 3688 4066 3688
Size: 378 6241
NspI r_CATG'y
Cuts at: 1132 1229 3977 5522 1132
Size: 97 2748 1545 2229
Fragments arranged by size:
2748 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2281 1348
Size: 933 5686
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 3322 1108
Size: 1608 606 4405
Fragments arranged by size:
4405 1608 606
PfoI T'CCnGG_A
Cuts at: 2172 2691 3633 3642 2172
Size: 519 942 9 5149
Fragments arranged by size:
5149 942 519 9
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 4688 4720 6201 6233 2468
Size: 32 2188 32 1481 32 2854
Fragments arranged by size:
2854 2188 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3319 3399 3319
Size: 80 6539
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 3890 3890
Size: 6619
PstI C_TGCA'G
Cuts at: 830 2077 2586 2795 3269 3371 3602 3790 3971
Size: 1247 509 209 474 102 231 188 181
Cuts at: 3971 830
Size: 3478
Fragments arranged by size:
3478 1247 509 474 231 209 188 181
102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 2311 2859 3202 3428 3574 2311
Size: 548 343 226 146 5356
Fragments arranged by size:
5356 548 343 226 146
SacI G_AGCT'C
Cuts at: 3528 3642 3528
Size: 114 6505
SacII CC_GC'GG
Cuts at: 2242 2742 2242
Size: 500 6119
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SbfI CC_TGCA'GG
Cuts at: 3371 3790 3971 3371
Size: 419 181 6019
Fragments arranged by size:
6019 419 181
ScaI AGT'ACT
Cuts at: 3263 4867 6579 3263
Size: 1604 1712 3303
Fragments arranged by size:
3303 1712 1604
SfcI C'TryA_G
Cuts at: 826 1045 2073 2582 2791 3265 3367 3598 3786
Size: 219 1028 509 209 474 102 231 188
Cuts at: 3786 3967 5102 5783 5974 826
Size: 181 1135 681 191 1471
Fragments arranged by size:
1471 1135 1028 681 509 474 231 219
209 191 188 181 102
SfoI GGC'GCC
Cuts at: 2218 3552 2218
Size: 1334 5285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 3119 1700
Size: 1419 5200
SmaI CCC'GGG
Cuts at: 1872 3946 1872
Size: 2074 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 3902 4723 5624 5886 820
Size: 197 926 309 1650 821 901 262 1553
Fragments arranged by size:
1650 1553 926 901 821 309 262 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3977 1132
Size: 2845 3774
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 3176 1517
Size: 1659 4960
StyI C'CwwG_G
Cuts at: 513 1065 2004 2127 3585 4058 513
Size: 552 939 123 1458 473 3074
Fragments arranged by size:
3074 1458 939 552 473 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2921 4646 4663 4816 5001 5446
Size: 1255 560 781 1725 17 153 185 445
Cuts at: 5446 325
Size: 1498
Fragments arranged by size:
1725 1498 1255 781 560 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 3261 3558
Size: 274 80 33 51 1443 513 771 297
Cuts at: 3558 4865 6577 96
Size: 1307 1712 138
Fragments arranged by size:
1712 1443 1307 771 513 297 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 6579 6611 3143
Size: 32 3404 32 3151
Fragments arranged by size:
3404 3151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2260 1316
Size: 944 5675
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3944 1870
Size: 2074 4545
XmnI GAAnn'nnTTC
Cuts at: 4748 4748
Size: 6619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2262 4427 276
Size: 53 83 186 936 728 2165 2468
Fragments arranged by size:
2468 2165 936 728 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI
XcmI