Restriction map for
pFN21AE5744
(Circular) (Six-base) MAPSORT of: pFN21AE5744.seq Check: 2083 from: 1 to: 6583
pFN21AE5744 6583 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3196 3517 3196
Size: 321 6262
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 3362 4710 5083 1563
Size: 1799 1348 373 3063
Fragments arranged by size:
3063 1799 1348 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 3155 3764 4646 6030 6298 1632
Size: 1379 144 609 882 1384 268 1917
Fragments arranged by size:
1917 1384 1379 882 609 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 6551
AlwNI CAG_nnn'CTG
Cuts at: 2263 2517 2710 3211 3496 4326 5898 2263
Size: 254 193 501 285 830 1572 2948
Fragments arranged by size:
2948 1572 830 501 285 254 193
ApaLI G'TGCA_C
Cuts at: 2585 4640 5796 2585
Size: 2055 1156 3372
Fragments arranged by size:
3372 2055 1156
ApoI r'AATT_y
Cuts at: 1493 2896 3788 3875 3892 4118 4270 6379 1493
Size: 1403 892 87 17 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 892 226 152 87 17
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2801 3444 3480 3567 3908 1870
Size: 73 96 762 643 36 87 341 4545
Fragments arranged by size:
4545 762 643 341 96 87 73 36
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2769 3904 5359 618
Size: 325 217 627 982 1135 1455 1842
Fragments arranged by size:
1842 1455 1135 982 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2220 2277 2478 2796 2826 3902 1391
Size: 829 57 201 318 30 1076 4072
Fragments arranged by size:
4072 1076 829 318 201 57 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2249 2925 3429 928
Size: 971 350 676 504 4082
Fragments arranged by size:
4082 971 676 504 350
BbvCI CC'TCA_GC
Cuts at: 3161 3593 3161
Size: 432 6151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2089 2123 2416 2450 2537 2571 4774
Size: 34 152 34 293 34 87 34 2203
Cuts at: 4774 4808 1903
Size: 34 3678
Fragments arranged by size:
3678 2203 293 152 87 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3062 4475 5685 3062
Size: 1413 1210 3960
Fragments arranged by size:
3960 1413 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2567 5194 136
Size: 107 122 71 631 605 895 2627 1525
Fragments arranged by size:
2627 1525 895 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 2589 4644 5800 1790
Size: 799 2055 1156 2573
Fragments arranged by size:
2573 2055 1156 799
BmgBI CAC'GTC
Cuts at: 1318 3226 1318
Size: 1908 4675
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4314 5272 442
Size: 416 53 83 399 24 2897 958 1753
Fragments arranged by size:
2897 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2492 3233 3266 3503 5243 1239
Size: 953 300 741 33 237 1740 2579
Fragments arranged by size:
2579 1740 953 741 300 237 33
Bpu10I CC'TnA_GC
Cuts at: 3161 3593 3161
Size: 432 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 2462 4708 5573 5871 2204
Size: 258 2246 865 298 2916
Fragments arranged by size:
2916 2246 865 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2969 5246 882
Size: 617 1470 2277 2219
Fragments arranged by size:
2277 2219 1470 617
BsaAI yAC'GTr
Cuts at: 493 1204 4374 493
Size: 711 3170 2702
Fragments arranged by size:
3170 2702 711
BsaBI GATnn'nnATC
Cuts at: 6577 6577
Size: 6583
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2832 3128 3762 4390
Size: 53 83 186 936 1299 296 634 628
Cuts at: 4390 4772 5407 275
Size: 382 635 1451
Fragments arranged by size:
1451 1299 936 635 634 628 382 296
186 83 53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3176 3206 1178
Size: 30 165 30 1773 30 4555
Fragments arranged by size:
4555 1773 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2252 2432 2516 3080 3356 3464 1186
Size: 904 162 180 84 564 276 108 4305
Fragments arranged by size:
4305 904 564 276 180 162 108 84
BseYI C'CCAG_C
Cuts at: 2693 3406 5786 6282 2693
Size: 713 2380 496 2994
Fragments arranged by size:
2994 2380 713 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2708 1163
Size: 573 345 627 5038
Fragments arranged by size:
5038 627 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2379 4033 4794 5822 1540
Size: 275 186 378 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 378 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2478 2589 2796 2826 4644 4729 5800 2220
Size: 258 111 207 30 1818 85 1071 3003
Fragments arranged by size:
3003 1818 1071 258 207 111 85 30
BsmI GAATG_Cn'
Cuts at: 2100 4094 4187 2100
Size: 1994 93 4496
Fragments arranged by size:
4496 1994 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2042 3135 2042
Size: 1093 5490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2277 2478 2589 2796 2826 3902
Size: 399 430 57 201 111 207 30 1076
Cuts at: 3902 4644 4729 5800 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1076 1071 742 430 399 207 201
111 85 57 30
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 3196 3468 3517 3938 844
Size: 1715 637 272 49 421 3489
Fragments arranged by size:
3489 1715 637 421 272 49
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 2767 4470 1394
Size: 429 329 615 1703 3507
Fragments arranged by size:
3507 1703 615 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 5078 5252 65
Size: 1183 3830 174 1396
Fragments arranged by size:
3830 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5227 5414 1700
Size: 110 3417 187 2869
Fragments arranged by size:
3417 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 2303 2411 2423 2486 1925
Size: 378 108 12 63 6022
Fragments arranged by size:
6022 378 108 63 12
BssSI C'ACGA_G
Cuts at: 2288 2970 4643 5655 2288
Size: 682 1673 1012 3216
Fragments arranged by size:
3216 1673 1012 682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 794
Size: 1469 5114
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6583
BstYI r'GATC_y
Cuts at: 1972 2466 3037 3913 4280 4665 4682 6134 6220
Size: 494 571 876 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 876 571 494 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3197 3197
Size: 6583
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5844
Fragments arranged by size:
5844 683 56
BtsI GCAGTG_nn'
Cuts at: 4099 4893 4913 4099
Size: 794 20 5769
Fragments arranged by size:
5769 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2376 4030
Size: 54 1425 50 275 18 237 309 1654
Cuts at: 4030 4919 6548 8
Size: 889 1629 43
Fragments arranged by size:
1654 1629 1425 889 309 275 237 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2376 4030 1537
Size: 275 564 1654 4090
Fragments arranged by size:
4090 1654 564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3660 3726 4513 1353
Size: 2307 66 787 3423
Fragments arranged by size:
3423 2307 787 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 2476 2794 2824 2218
Size: 258 318 30 5977
Fragments arranged by size:
5977 318 258 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2492 3011 3155 3233 3266 3503
Size: 393 560 300 519 144 78 33 237
Cuts at: 3503 3764 4646 5243 6030 6298 1239
Size: 261 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 597 560 519 393 300
268 261 237 144 78 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3518 3518
Size: 6583
EcoO109I rG'GnC_Cy
Cuts at: 2430 3402 3493 2430
Size: 972 91 5520
Fragments arranged by size:
5520 972 91
EcoRI G'AATT_C
Cuts at: 2896 3788 3892 4270 2896
Size: 892 104 378 5209
Fragments arranged by size:
5209 892 378 104
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 2264 2399 2449 2510 5730 1678
Size: 586 135 50 61 3220 2531
Fragments arranged by size:
3220 2531 586 135 61 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3224 3256 3329 3361 3807
Size: 32 135 32 1652 32 73 32 446
Cuts at: 3807 3839 5224 5256 5298 5330 1373
Size: 32 1385 32 42 32 2626
Fragments arranged by size:
2626 1652 1385 446 135 73 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3927 4173 669
Size: 988 281 1989 246 3079
Fragments arranged by size:
3079 1989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5697 5881 1305
Size: 4392 184 2007
Fragments arranged by size:
4392 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 10
Size: 54 1425 343 237 4524
Fragments arranged by size:
4524 1425 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4541 4900 5059 518
Size: 648 3375 359 159 2042
Fragments arranged by size:
3375 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2743 2989 3475 3676 3742 3999 4676
Size: 732 405 246 486 201 66 257 677
Cuts at: 4676 5824 6069 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 486 405 257 246
245 201 66
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6031
NdeI CA'TA_TG
Cuts at: 387 2847 387
Size: 2460 4123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 2524 3314 3941 5486 1132
Size: 97 1295 790 627 1545 2229
Fragments arranged by size:
2229 1545 1295 790 627 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3061 1108
Size: 1953 4630
PfoI T'CCnGG_A
Cuts at: 2469 2835 3519 2469
Size: 366 684 5533
Fragments arranged by size:
5533 684 366
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 4652 4684 6165 6197 3176
Size: 32 1444 32 1481 32 3562
Fragments arranged by size:
3562 1481 1444 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3402 3402
Size: 6583
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PstI C_TGCA'G
Cuts at: 830 2160 2259 2337 2460 2721 3207 3935 830
Size: 1330 99 78 123 261 486 728 3478
Fragments arranged by size:
3478 1330 728 486 261 123 99 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2743 2989 3475 3676 3742 2743
Size: 246 486 201 66 5584
Fragments arranged by size:
5584 486 246 201 66
SacI G_AGCT'C
Cuts at: 2220 2478 2796 2826 2220
Size: 258 318 30 5977
Fragments arranged by size:
5977 318 258 30
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SfcI C'TryA_G
Cuts at: 826 1045 2156 2255 2333 2342 2456 2717 3203
Size: 219 1111 99 78 9 114 261 486
Cuts at: 3203 3931 5066 5747 5938 826
Size: 728 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 728 681 486 261 219
191 114 99 78 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 2803 3482 3910 1872
Size: 931 679 428 4545
Fragments arranged by size:
4545 931 679 428
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2219 2477 4687 5588 5850 820
Size: 197 926 276 258 2210 901 262 1553
Fragments arranged by size:
2210 1553 926 901 276 262 258 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 2524 3941 1132
Size: 1392 1417 3774
Fragments arranged by size:
3774 1417 1392
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6583
StyI C'CwwG_G
Cuts at: 513 1065 2247 2538 2667 3765 4022 513
Size: 552 1182 291 129 1098 257 3074
Fragments arranged by size:
3074 1182 1098 552 291 257 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 2993 4610 4627 4780 4965 5410
Size: 1255 840 573 1617 17 153 185 445
Cuts at: 5410 325
Size: 1498
Fragments arranged by size:
1617 1498 1255 840 573 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4829 6541 96
Size: 274 80 33 51 1443 2852 1712 138
Fragments arranged by size:
2852 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2638 2670 3641 3673 6543 6575 2638
Size: 32 971 32 2870 32 2646
Fragments arranged by size:
2870 2646 971 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2384 1316
Size: 1068 5515
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 2801 3480 3908 1870
Size: 931 679 428 4545
Fragments arranged by size:
4545 931 679 428
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI