Restriction map for
pFN21AE5741
(Circular) (Six-base) MAPSORT of: pFN21AE5741.seq Check: 5141 from: 1 to: 6574
pFN21AE5741 6574 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2875 2875
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2156 2420 2676 3334 3882 4637 6021
Size: 404 120 264 256 658 548 755 1384
Cuts at: 6021 6289 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 755 658 548 404 268 264
256 120
AflII C'TTAA_G
Cuts at: 820 1017 2509 820
Size: 197 1492 4885
Fragments arranged by size:
4885 1492 197
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 2719 5303 2719
Size: 2584 3990
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 2425 4317 5889 2425
Size: 1892 1572 3110
Fragments arranged by size:
3110 1892 1572
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 3215 3315 3883 4109 4261 6370 1493
Size: 1722 100 568 226 152 2109 1697
Fragments arranged by size:
2109 1722 1697 568 226 152 100
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3899 1870
Size: 73 1956 4545
Fragments arranged by size:
4545 1956 73
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3895 5350 618
Size: 325 217 627 2108 1455 1842
Fragments arranged by size:
2108 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2976 3893 1391
Size: 1585 917 4072
Fragments arranged by size:
4072 1585 917
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2368 2574 2682 2924 3663 3783 3834
Size: 971 469 206 108 242 739 120 51
Cuts at: 3834 928
Size: 3668
Fragments arranged by size:
3668 971 739 469 242 206 120 108
51
BbvCI CC'TCA_GC
Cuts at: 2031 2073 3720 2031
Size: 42 1647 4885
Fragments arranged by size:
4885 1647 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 2022 1548
Size: 474 6100
BfrBI ATG'CAT
Cuts at: 3626 3626
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3865 6569 3865
Size: 2704 3870
BlpI GC'TnA_GC
Cuts at: 2429 3991 2429
Size: 1562 5012
Bme1580I G_kGCm'C
Cuts at: 1790 3138 4635 5791 1790
Size: 1348 1497 1156 2573
Fragments arranged by size:
2573 1497 1348 1156
BmgBI CAC'GTC
Cuts at: 1318 3745 1318
Size: 2427 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2745 3080 4305
Size: 416 53 83 399 24 1328 335 1225
Cuts at: 4305 5263 442
Size: 958 1753
Fragments arranged by size:
1753 1328 1225 958 416 399 335 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3075 3075
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2393 2571 5234 1239
Size: 1139 15 178 2663 2579
Fragments arranged by size:
2663 2579 1139 178 15
Bpu10I CC'TnA_GC
Cuts at: 2031 2073 3720 2031
Size: 42 1647 4885
Fragments arranged by size:
4885 1647 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2484 4699 5564 5862 2484
Size: 2215 865 298 3196
Fragments arranged by size:
3196 2215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 882
Size: 617 3738 2219
Fragments arranged by size:
3738 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4365 493
Size: 711 3161 2702
Fragments arranged by size:
3161 2702 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 4763 5398 275
Size: 53 83 186 936 2848 382 635 1451
Fragments arranged by size:
2848 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5007 5405 5679 5826 6237 5007
Size: 398 274 147 411 5344
Fragments arranged by size:
5344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2600 2630 1178
Size: 30 165 30 1197 30 5122
Fragments arranged by size:
5122 1197 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2061 2608 2620 2744 1186
Size: 875 547 12 124 5016
Fragments arranged by size:
5016 875 547 124 12
BseYI C'CCAG_C
Cuts at: 2364 3124 5777 6273 2364
Size: 760 2653 496 2665
Fragments arranged by size:
2665 2653 760 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3386 1163
Size: 573 1650 4351
Fragments arranged by size:
4351 1650 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4635 4720 5791 4635
Size: 85 1071 5418
Fragments arranged by size:
5418 1071 85
BsmI GAATG_Cn'
Cuts at: 3382 4085 4178 3382
Size: 703 93 5778
Fragments arranged by size:
5778 703 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2976 3138 3893 4635 4720 5791 1391
Size: 399 1186 162 755 742 85 1071 2174
Fragments arranged by size:
2174 1186 1071 755 742 399 162 85
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2875 3929 844
Size: 2031 1054 3489
Fragments arranged by size:
3489 2031 1054
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 3697 5069 5243 65
Size: 1183 1698 751 1372 174 1396
Fragments arranged by size:
1698 1396 1372 1183 751 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5218 5405 1700
Size: 110 3408 187 2869
Fragments arranged by size:
3408 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2425 2890 794
Size: 1631 465 4478
Fragments arranged by size:
4478 1631 465
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 2046 3865 3904 4271 4656 4673 6125 6211
Size: 74 1819 39 367 385 17 1452 86
Cuts at: 6211 6223 6569 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1819 1452 385 367 346 86 74
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2761 513
Size: 552 1696 4326
Fragments arranged by size:
4326 1696 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 2859 4090 4884 4904 2859
Size: 1231 794 20 4529
Fragments arranged by size:
4529 1231 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2820 3878 2820
Size: 1058 5516
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2467 2758 2777
Size: 54 1425 50 275 18 637 291 19
Cuts at: 2777 4021 4910 6539 8
Size: 1244 889 1629 43
Fragments arranged by size:
1629 1425 1244 889 637 291 275 54
50 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3747 3843 4504 1353
Size: 2394 96 661 3423
Fragments arranged by size:
3423 2394 661 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2156 2378 2393 2420 2571 2676
Size: 393 404 120 222 15 27 151 105
Cuts at: 2676 3334 3882 4637 5234 6021 6289 1239
Size: 658 548 755 597 787 268 1524
Fragments arranged by size:
1524 787 755 658 597 548 404 393
268 222 151 120 105 27 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3641 3641
Size: 6574
EcoO109I rG'GnC_Cy
Cuts at: 2739 3107 2739
Size: 368 6206
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 5721 1678
Size: 4043 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 2705 2737 3842
Size: 32 135 32 442 32 659 32 1105
Cuts at: 3842 3874 5215 5247 5289 5321 1373
Size: 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1341 1105 659 442 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3918 4164 669
Size: 988 281 1365 615 246 3079
Fragments arranged by size:
3079 1365 988 615 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 3303 4164 3303
Size: 861 5713
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 2354 4173 2354
Size: 1819 4755
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2119 2658 3015 3527 5688 5872 1305
Size: 814 539 357 512 2161 184 2007
Fragments arranged by size:
2161 2007 814 539 512 357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2469 2760 2779 10
Size: 54 1425 343 637 291 19 3805
Fragments arranged by size:
3805 1425 637 343 291 54 19
MslI CAynn'nnrTG
Cuts at: 518 1166 3185 4532 4891 5050 518
Size: 648 2019 1347 359 159 2042
Fragments arranged by size:
2042 2019 1347 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3324 3990 4667 5815 6060 1606
Size: 1718 666 677 1148 245 2120
Fragments arranged by size:
2120 1718 1148 677 666 245
NcoI C'CATG_G
Cuts at: 513 1065 2761 513
Size: 552 1696 4326
Fragments arranged by size:
4326 1696 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NsiI A_TGCA'T
Cuts at: 3628 3628
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2962 3932 5477 1132
Size: 97 1733 970 1545 2229
Fragments arranged by size:
2229 1733 1545 970 97
PasI CC'CwG_GG
Cuts at: 1348 3103 1348
Size: 1755 4819
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6574
PfoI T'CCnGG_A
Cuts at: 2305 3607 2305
Size: 1302 5272
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3107 3107
Size: 6574
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 3222 4144 3222
Size: 922 5652
PstI C_TGCA'G
Cuts at: 830 2121 3645 3926 830
Size: 1291 1524 281 3478
Fragments arranged by size:
3478 1524 1291 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 3324 3324
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SanDI GG'GwC_CC
Cuts at: 3107 3107
Size: 6574
SapI GCTCTTCn'nnn_
Cuts at: 3747 3843 3747
Size: 96 6478
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SfcI C'TryA_G
Cuts at: 826 1045 2117 3641 3922 5057 5738 5929 826
Size: 219 1072 1524 281 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 1072 681 281 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 2509 4678 5579 5841 820
Size: 197 926 556 10 2169 901 262 1553
Fragments arranged by size:
2169 1553 926 901 556 262 197 10
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2761 3573 4013 513
Size: 552 1696 812 440 3074
Fragments arranged by size:
3074 1696 812 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2868 4601 4618 4771 4956 5401 325
Size: 1255 1288 1733 17 153 185 445 1498
Fragments arranged by size:
1733 1498 1288 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 6534 6566 2469
Size: 32 4033 32 2477
Fragments arranged by size:
4033 2477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 2931 4703 2931
Size: 1772 4802
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI
SfiI SfoI SrfI SwaI XcmI