Restriction map for
pFN21AE5737
(Circular) (Six-base) MAPSORT of: pFN21AE5737.seq Check: 7100 from: 1 to: 6985
pFN21AE5737 6985 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3801 4795 278
Size: 53 83 186 936 2265 994 2468
Fragments arranged by size:
2468 2265 994 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4328 1937
Size: 2391 4594
Acc65I G'GTAC_C
Cuts at: 3744 4306 3744
Size: 562 6423
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 2701 2888 3487 4097 5048 6432 6700
Size: 686 383 187 599 610 951 1384 268
Cuts at: 6700 1632
Size: 1917
Fragments arranged by size:
1917 1384 951 686 610 599 383 268
187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6788
AflIII A'CryG_T
Cuts at: 1203 5884 6861 1203
Size: 4681 977 1327
Fragments arranged by size:
4681 1327 977
AgeI A'CCGG_T
Cuts at: 5816 5816
Size: 6985
AhdI GACnn_n'nnGTC
Cuts at: 5714 5714
Size: 6985
AleI CACnn'nnGTG
Cuts at: 1166 2636 1166
Size: 1470 5515
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6985
AlwNI CAG_nnn'CTG
Cuts at: 2786 3510 4180 4728 6300 2786
Size: 724 670 548 1572 3471
Fragments arranged by size:
3471 1572 724 670 548
ApaLI G'TGCA_C
Cuts at: 2687 2811 5042 6198 2687
Size: 124 2231 1156 3474
Fragments arranged by size:
3474 2231 1156 124
ApoI r'AATT_y
Cuts at: 1493 4294 4520 4672 6781 1493
Size: 2801 226 152 2109 1697
Fragments arranged by size:
2801 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5539 160
Size: 872 4507 1606
Fragments arranged by size:
4507 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 4046 4310 1870
Size: 73 2103 264 4545
Fragments arranged by size:
4545 2103 264 73
BamHI G'GATC_C
Cuts at: 4315 4682 4315
Size: 367 6618
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2438 3744 4306 5761
Size: 325 217 627 462 189 1306 562 1455
Cuts at: 5761 618
Size: 1842
Fragments arranged by size:
1842 1455 1306 627 562 462 325 217
189
BanII G_rGCy'C
Cuts at: 1391 3195 3226 4304 1391
Size: 1804 31 1078 4072
Fragments arranged by size:
4072 1804 1078 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2876 3016 3225 928
Size: 971 977 140 209 4688
Fragments arranged by size:
4688 977 971 209 140
BbvCI CC'TCA_GC
Cuts at: 4100 4100
Size: 6985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 5176 5210 1903
Size: 34 1904 34 1301 34 3678
Fragments arranged by size:
3678 1904 1301 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4877 6087 4877
Size: 1210 5775
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6985
BfrBI ATG'CAT
Cuts at: 3698 3698
Size: 6985
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 4250 5596
Size: 107 122 71 631 605 1231 1347 1346
Cuts at: 5596 136
Size: 1525
Fragments arranged by size:
1525 1347 1346 1231 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6980 6980
Size: 6985
BlpI GC'TnA_GC
Cuts at: 4402 4402
Size: 6985
Bme1580I G_kGCm'C
Cuts at: 1790 2252 2691 2815 3657 5046 6202 1790
Size: 462 439 124 842 1389 1156 2573
Fragments arranged by size:
2573 1389 1156 842 462 439 124
BmgBI CAC'GTC
Cuts at: 1318 3760 3868 1318
Size: 2442 108 4435
Fragments arranged by size:
4435 2442 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4172 4716 5674
Size: 416 53 83 399 24 2755 544 958
Cuts at: 5674 442
Size: 1753
Fragments arranged by size:
2755 1753 958 544 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2811 3137 3662 3904 5645 1239
Size: 1572 326 525 242 1741 2579
Fragments arranged by size:
2579 1741 1572 525 326 242
Bpu10I CC'TnA_GC
Cuts at: 2111 4100 2111
Size: 1989 4996
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3248 5110 5975 6273 3248
Size: 1862 865 298 3960
Fragments arranged by size:
3960 1862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 3881 3922 5648 882
Size: 617 858 1524 41 1726 2219
Fragments arranged by size:
2219 1726 1524 858 617 41
BsaAI yAC'GTr
Cuts at: 493 1204 2692 4776 493
Size: 711 1488 2084 2702
Fragments arranged by size:
2702 2084 1488 711
BsaBI GATnn'nnATC
Cuts at: 6979 6979
Size: 6985
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3798 4792 5174 5809
Size: 53 83 186 936 2265 994 382 635
Cuts at: 5809 275
Size: 1451
Fragments arranged by size:
2265 1451 994 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2654 4155 5418 5816 6090 6237 6648 2654
Size: 1501 1263 398 274 147 411 2991
Fragments arranged by size:
2991 1501 1263 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2784 2814 1178
Size: 30 165 30 1381 30 5349
Fragments arranged by size:
5349 1381 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3970 1186
Size: 2784 4201
BseYI C'CCAG_C
Cuts at: 2258 3097 3237 3416 6188 6684 2258
Size: 839 140 179 2772 496 2559
Fragments arranged by size:
2772 2559 839 496 179 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3094 3535 3546 1163
Size: 573 1358 441 11 4602
Fragments arranged by size:
4602 1358 573 441 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3302 4435 5196 6224 1540
Size: 275 186 1301 1133 761 1028 2301
Fragments arranged by size:
2301 1301 1133 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 2815 3185 3195 3840 5046 5131 6202 2691
Size: 124 370 10 645 1206 85 1071 3474
Fragments arranged by size:
3474 1206 1071 645 370 124 85 10
BsmI GAATG_Cn'
Cuts at: 4496 4589 4496
Size: 93 6892
BsmBI CGTCTCn'nnnn_
Cuts at: 2954 3866 2954
Size: 912 6073
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2691 2815 3185 3195 3226 3657
Size: 399 462 439 124 370 10 31 431
Cuts at: 3657 3840 4304 5046 5131 6202 1391
Size: 183 464 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 462 439 431 399
370 183 124 85 31 10
BspEI T'CCGG_A
Cuts at: 4155 6648 4155
Size: 2493 4492
BspHI T'CATG_A
Cuts at: 4874 6604 4874
Size: 1730 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4340 844
Size: 3496 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2577 4194 4872 1394
Size: 429 754 1617 678 3507
Fragments arranged by size:
3507 1617 754 678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3150 3561 5480 5654 65
Size: 1183 1902 411 1919 174 1396
Fragments arranged by size:
1919 1902 1396 1183 411 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3297 3301 4054 5629 5816 1700
Size: 110 1487 4 753 1575 187 2869
Fragments arranged by size:
2869 1575 1487 753 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 4612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6985
BssSI C'ACGA_G
Cuts at: 5045 6057 5045
Size: 1012 5973
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2534 2786 794
Size: 1740 252 4993
Fragments arranged by size:
4993 1740 252
BstBI TT'CG_AA
Cuts at: 5789 5789
Size: 6985
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6985
BstXI CCAn_nnnn'nTGG
Cuts at: 3097 3740 3097
Size: 643 6342
BstYI r'GATC_y
Cuts at: 1972 2921 3393 4315 4682 5067 5084 6536 6622
Size: 949 472 922 367 385 17 1452 86
Cuts at: 6622 6634 6980 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 949 922 472 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 3909 513
Size: 552 939 273 1632 3589
Fragments arranged by size:
3589 1632 939 552 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 3049 487
Size: 683 56 810 1013 4423
Fragments arranged by size:
4423 1013 810 683 56
BtsI GCAGTG_nn'
Cuts at: 2348 3141 4181 4501 5295 5315 2348
Size: 793 1040 320 794 20 4018
Fragments arranged by size:
4018 1040 794 793 320 20
ClaI AT'CG_AT
Cuts at: 1554 2268 1554
Size: 714 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3199 3234 529
Size: 35 2635 35 4280
Fragments arranged by size:
4280 2635 35 35
DraI TTT'AAA
Cuts at: 4289 4289
Size: 6985
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5992 809
Size: 507 4676 1802
Fragments arranged by size:
4676 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3299 3324 3789
Size: 54 1425 50 275 18 1469 25 465
Cuts at: 3789 4056 4432 5321 6950 8
Size: 267 376 889 1629 43
Fragments arranged by size:
1629 1469 1425 889 465 376 275 267
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3299 4432 1537
Size: 275 1487 1133 4090
Fragments arranged by size:
4090 1487 1133 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2838 3005 3053 3413 4915 1353
Size: 1485 167 48 360 1502 3423
Fragments arranged by size:
3423 1502 1485 360 167 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5572 5946 6092 387
Size: 1064 138 3983 374 146 1280
Fragments arranged by size:
3983 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3193 3193
Size: 6985
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2701 2811 2888 3137 3487 3662
Size: 393 686 383 110 77 249 350 175
Cuts at: 3662 3904 4097 5048 5645 6432 6700 1239
Size: 242 193 951 597 787 268 1524
Fragments arranged by size:
1524 951 787 686 597 393 383 350
268 249 242 193 175 110 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2669 2878 3073 2300
Size: 369 209 195 6212
Fragments arranged by size:
6212 369 209 195
EcoO109I rG'GnC_Cy
Cuts at: 4261 4261
Size: 6985
EcoRI G'AATT_C
Cuts at: 4294 4672 4294
Size: 378 6607
EcoRV GAT'ATC
Cuts at: 4150 4150
Size: 6985
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 2486 3937 6132 1678
Size: 808 1451 2195 2531
Fragments arranged by size:
2531 2195 1451 808
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 4253 4285 5626
Size: 32 135 32 620 32 2029 32 1341
Cuts at: 5626 5658 5700 5732 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2029 1341 620 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4329 4575 669
Size: 988 281 2391 246 3079
Fragments arranged by size:
3079 2391 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6985
HpaI GTT'AAC
Cuts at: 4575 4575
Size: 6985
KpnI G_GTAC'C
Cuts at: 3748 4310 3748
Size: 562 6423
MfeI C'AATT_G
Cuts at: 2817 4584 2817
Size: 1767 5218
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 6099 6283 1305
Size: 1086 3708 184 2007
Fragments arranged by size:
3708 2007 1086 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3791 10
Size: 54 1425 343 1959 3204
Fragments arranged by size:
3204 1959 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 3128 3326 3425 3486 3738 4130
Size: 648 1470 492 198 99 61 252 392
Cuts at: 4130 4943 5302 5461 518
Size: 813 359 159 2042
Fragments arranged by size:
2042 1470 813 648 492 392 359 252
198 159 99 61
MspA1I CmG'CkG
Cuts at: 1606 2777 2783 3057 3337 3472 4401 5078 6226
Size: 1171 6 274 280 135 929 677 1148
Cuts at: 6226 6471 1606
Size: 245 2120
Fragments arranged by size:
2120 1171 1148 929 677 280 274 245
135 6
NaeI GCC'GGC
Cuts at: 3303 4056 3303
Size: 753 6232
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5494
Fragments arranged by size:
5494 939 552
NdeI CA'TA_TG
Cuts at: 387 2174 2533 4239 387
Size: 1787 359 1706 3133
Fragments arranged by size:
3133 1787 1706 359
NgoMIV G'CCGG_C
Cuts at: 3301 4054 3301
Size: 753 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 4432 4432
Size: 6985
NsiI A_TGCA'T
Cuts at: 3700 3700
Size: 6985
NspI r_CATG'y
Cuts at: 1132 1229 4343 5888 1132
Size: 97 3114 1545 2229
Fragments arranged by size:
3114 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6985
PciI A'CATG_T
Cuts at: 5884 5884
Size: 6985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2106 3684 1108
Size: 998 1578 4409
Fragments arranged by size:
4409 1578 998
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PmlI CAC'GTG
Cuts at: 2692 2692
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3954 3986 5054 5086 6567 6599 3954
Size: 32 1068 32 1481 32 4340
Fragments arranged by size:
4340 1481 1068 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4261 4261
Size: 6985
PshAI GACnn'nnGTC
Cuts at: 1613 2388 1613
Size: 775 6210
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PstI C_TGCA'G
Cuts at: 830 2782 4337 830
Size: 1952 1555 3478
Fragments arranged by size:
3478 1952 1555
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 2777 3337 3472 2777
Size: 560 135 6290
Fragments arranged by size:
6290 560 135
SacI G_AGCT'C
Cuts at: 3195 3195
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SbfI CC_TGCA'GG
Cuts at: 4337 4337
Size: 6985
ScaI AGT'ACT
Cuts at: 5233 6945 5233
Size: 1712 5273
SfcI C'TryA_G
Cuts at: 826 1045 2778 4333 5468 6149 6340 826
Size: 219 1733 1555 1135 681 191 1471
Fragments arranged by size:
1733 1555 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 4312 1872
Size: 2440 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3227 5089 5990 6252 820
Size: 197 926 1284 1862 901 262 1553
Fragments arranged by size:
1862 1553 1284 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4776 493
Size: 4283 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 4343 1132
Size: 3211 3774
SspI AAT'ATT
Cuts at: 5 52 4909 5
Size: 47 4857 2081
Fragments arranged by size:
4857 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6985
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 2665 4424 513
Size: 552 939 249 412 1759 3074
Fragments arranged by size:
3074 1759 939 552 412 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2206 3857 3905 5012 5029 5182 5367
Size: 1255 626 1651 48 1107 17 153 185
Cuts at: 5367 5812 325
Size: 445 1498
Fragments arranged by size:
1651 1498 1255 1107 626 445 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 2736 3645
Size: 274 80 33 51 1443 492 267 909
Cuts at: 3645 4026 5231 6943 96
Size: 381 1205 1712 138
Fragments arranged by size:
1712 1443 1205 909 492 381 274 267
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2205 2237 3447 3479 6945 6977 2205
Size: 32 1210 32 3466 32 2213
Fragments arranged by size:
3466 2213 1210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3533 3601 1316
Size: 2217 68 4700
Fragments arranged by size:
4700 2217 68
XbaI T'CTAG_A
Cuts at: 2839 4321 2839
Size: 1482 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3334 3424 3334
Size: 90 6895
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 4310 1870
Size: 2440 4545
XmnI GAAnn'nnTTC
Cuts at: 3966 5114 3966
Size: 1148 5837
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3799 4793 276
Size: 53 83 186 936 2265 994 2468
Fragments arranged by size:
2468 2265 994 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI
KasI NarI NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI