Restriction map for
pFN21AE5736
(Circular) (Six-base) MAPSORT of: pFN21AE5736.seq Check: 3261 from: 1 to: 7201
pFN21AE5736 7201 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5011 278
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4190 4375 4544 1937
Size: 2253 185 169 4594
Fragments arranged by size:
4594 2253 185 169
Acc65I G'GTAC_C
Cuts at: 4522 4522
Size: 7201
AclI AA'CG_TT
Cuts at: 1563 3318 5328 5701 1563
Size: 1755 2010 373 3063
Fragments arranged by size:
3063 2010 1755 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1997 2659 3405 4500 5264 6648 6916 1632
Size: 365 662 746 1095 764 1384 268 1917
Fragments arranged by size:
1917 1384 1095 764 746 662 365 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7004
AflIII A'CryG_T
Cuts at: 1203 3020 3709 6100 7077 1203
Size: 1817 689 2391 977 1327
Fragments arranged by size:
2391 1817 1327 977 689
AgeI A'CCGG_T
Cuts at: 6032 6032
Size: 7201
AhdI GACnn_n'nnGTC
Cuts at: 5930 5930
Size: 7201
AleI CACnn'nnGTG
Cuts at: 1166 2690 2786 3755 1166
Size: 1524 96 969 4612
Fragments arranged by size:
4612 1524 969 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7201
AlwNI CAG_nnn'CTG
Cuts at: 3578 4944 6516 3578
Size: 1366 1572 4263
Fragments arranged by size:
4263 1572 1366
ApaLI G'TGCA_C
Cuts at: 2808 4047 5258 6414 2808
Size: 1239 1211 1156 3595
Fragments arranged by size:
3595 1239 1211 1156
ApoI r'AATT_y
Cuts at: 1493 2668 2979 3462 4510 4736 4888 6997 1493
Size: 1175 311 483 1048 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 1048 483 311 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3003 5755 160
Size: 872 1971 2752 1606
Fragments arranged by size:
2752 1971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
AvaI C'yCGr_G
Cuts at: 1870 1943 2580 4526 1870
Size: 73 637 1946 4545
Fragments arranged by size:
4545 1946 637 73
BamHI G'GATC_C
Cuts at: 3778 4531 4898 3778
Size: 753 367 6081
Fragments arranged by size:
6081 753 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4522 5977 618
Size: 325 217 627 2735 1455 1842
Fragments arranged by size:
2735 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 4520 1391
Size: 2559 570 4072
Fragments arranged by size:
4072 2559 570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2030 3393 3930 928
Size: 971 131 1363 537 4199
Fragments arranged by size:
4199 1363 971 537 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2402 2436 4079 4113 5392 5426 1903
Size: 34 465 34 1643 34 1279 34 3678
Fragments arranged by size:
3678 1643 1279 465 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4341 5093 6303 4341
Size: 752 1210 5239
Fragments arranged by size:
5239 1210 752
BclI T'GATC_A
Cuts at: 1548 2523 3334 4084 1548
Size: 975 811 750 4665
Fragments arranged by size:
4665 975 811 750
BfrBI ATG'CAT
Cuts at: 2918 2918
Size: 7201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5812 136
Size: 107 122 71 631 605 4140 1525
Fragments arranged by size:
4140 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2270 3858 7196 2270
Size: 1588 3338 2275
Fragments arranged by size:
3338 2275 1588
BlpI GC'TnA_GC
Cuts at: 4618 4618
Size: 7201
Bme1580I G_kGCm'C
Cuts at: 1790 2812 4051 5262 6418 1790
Size: 1022 1239 1211 1156 2573
Fragments arranged by size:
2573 1239 1211 1156 1022
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2202 3077 3459
Size: 416 53 83 399 24 785 875 382
Cuts at: 3459 4159 4353 4932 5890 442
Size: 700 194 579 958 1753
Fragments arranged by size:
1753 958 875 785 700 579 416 399
382 194 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4007 1056
Size: 2951 4250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2369 5861 1239
Size: 1031 99 3492 2579
Fragments arranged by size:
3492 2579 1031 99
Bpu10I CC'TnA_GC
Cuts at: 3612 3612
Size: 7201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4455 5326 6191 6489 3964
Size: 491 871 865 298 4676
Fragments arranged by size:
4676 871 865 491 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5864 882
Size: 617 4365 2219
Fragments arranged by size:
4365 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2884 3712 3847 4992 493
Size: 711 1680 828 135 1145 2702
Fragments arranged by size:
2702 1680 1145 828 711 135
BsaBI GATnn'nnATC
Cuts at: 2507 3344 4083 4238 7195 2507
Size: 837 739 155 2957 2513
Fragments arranged by size:
2957 2513 837 739 155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 5008 5390 6025
Size: 53 83 186 936 1966 1509 382 635
Cuts at: 6025 275
Size: 1451
Fragments arranged by size:
1966 1509 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3533 5634 6032 6306 6453 6864 3533
Size: 2101 398 274 147 411 3870
Fragments arranged by size:
3870 2101 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4205 4235 1178
Size: 30 165 30 2802 30 4144
Fragments arranged by size:
4144 2802 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3240 4226 1186
Size: 2054 986 4161
Fragments arranged by size:
4161 2054 986
BseYI C'CCAG_C
Cuts at: 6404 6900 6404
Size: 496 6705
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3078 1163
Size: 573 1342 5286
Fragments arranged by size:
5286 1342 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4651 5412 6440 1540
Size: 275 186 2650 761 1028 2301
Fragments arranged by size:
2650 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2812 3950 4051 4471 5262 5347 6418 2812
Size: 1138 101 420 791 85 1071 3595
Fragments arranged by size:
3595 1138 1071 791 420 101 85
BsmI GAATG_Cn'
Cuts at: 4712 4805 4712
Size: 93 7108
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2812 3950 4051 4471 4520 5262 5347
Size: 399 1022 1138 101 420 49 742 85
Cuts at: 5347 6418 1391
Size: 1071 2174
Fragments arranged by size:
2174 1138 1071 1022 742 420 399 101
85 49
BspEI T'CCGG_A
Cuts at: 6864 6864
Size: 7201
BspHI T'CATG_A
Cuts at: 5090 6820 5090
Size: 1730 5471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4322 4556 844
Size: 3478 234 3489
Fragments arranged by size:
3489 3478 234
BsrBI CCG'CTC
Cuts at: 1394 1823 5088 1394
Size: 429 3265 3507
Fragments arranged by size:
3507 3265 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3769 5696 5870 65
Size: 1183 2521 1927 174 1396
Fragments arranged by size:
2521 1927 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5845 6032 1700
Size: 110 4035 187 2869
Fragments arranged by size:
4035 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7201
BssSI C'ACGA_G
Cuts at: 5261 6273 5261
Size: 1012 6189
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7201
BstBI TT'CG_AA
Cuts at: 6005 6005
Size: 7201
BstEII G'GTnAC_C
Cuts at: 1184 4334 1184
Size: 3150 4051
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2692 3851 4393 2461
Size: 231 1159 542 5269
Fragments arranged by size:
5269 1159 542 231
BstYI r'GATC_y
Cuts at: 1972 2270 2588 3339 3517 3778 3858 4531 4898
Size: 298 318 751 178 261 80 673 367
Cuts at: 4898 5283 5300 6752 6838 6850 7196 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 751 673 385 367 346 318
298 261 178 86 80 17 12
BstZ17I GTA'TAC
Cuts at: 4376 4376
Size: 7201
BtgI C'CryG_G
Cuts at: 513 1065 3171 513
Size: 552 2106 4543
Fragments arranged by size:
4543 2106 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6462
Fragments arranged by size:
6462 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4717 5511 5531 4135
Size: 582 794 20 5805
Fragments arranged by size:
5805 794 582 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2718 2753 529
Size: 35 2154 35 4977
Fragments arranged by size:
4977 2154 35 35
DraI TTT'AAA
Cuts at: 4505 4505
Size: 7201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6208 809
Size: 507 4892 1802
Fragments arranged by size:
4892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2472 2485
Size: 54 1425 50 275 18 238 404 13
Cuts at: 2485 3918 4256 4325 4648 5537 7166 8
Size: 1433 338 69 323 889 1629 43
Fragments arranged by size:
1629 1433 1425 889 404 338 323 275
238 69 54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 4648 1537
Size: 275 2836 4090
Fragments arranged by size:
4090 2836 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3188 5131 1353
Size: 1835 1943 3423
Fragments arranged by size:
3423 1943 1835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5788 6162 6308 387
Size: 1064 138 4199 374 146 1280
Fragments arranged by size:
4199 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3948 3948
Size: 7201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1997 2270 2369 2659 3405 4500 5264
Size: 393 365 273 99 290 746 1095 764
Cuts at: 5264 5861 6648 6916 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1095 787 764 746 597 393 365
290 273 268 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2257 2257
Size: 7201
EcoO109I rG'GnC_Cy
Cuts at: 3084 4103 3084
Size: 1019 6182
EcoRI G'AATT_C
Cuts at: 2668 4510 4888 2668
Size: 1842 378 4981
Fragments arranged by size:
4981 1842 378
EcoRV GAT'ATC
Cuts at: 2698 3328 2698
Size: 630 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 7201
FspI TGC'GCA
Cuts at: 5707 5707
Size: 7201
HaeII r_GCGC'y
Cuts at: 1678 6348 1678
Size: 4670 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 5842 5874 5916
Size: 32 135 32 1100 32 3138 32 42
Cuts at: 5916 5948 1373
Size: 32 2626
Fragments arranged by size:
3138 2626 1100 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3511 4545 4791 669
Size: 988 281 889 684 1034 246 3079
Fragments arranged by size:
3079 1034 988 889 684 281 246
HindIII A'AGCT_T
Cuts at: 748 4135 748
Size: 3387 3814
HpaI GTT'AAC
Cuts at: 4791 4791
Size: 7201
KpnI G_GTAC'C
Cuts at: 4526 4526
Size: 7201
MfeI C'AATT_G
Cuts at: 2859 4800 2859
Size: 1941 5260
MluI A'CGCG_T
Cuts at: 7077 7077
Size: 7201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3609 6315 6499 1305
Size: 2304 2706 184 2007
Fragments arranged by size:
2706 2304 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2070 2487 3920 10
Size: 54 1425 343 238 417 1433 3291
Fragments arranged by size:
3291 1433 1425 417 343 238 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2348 2690 2786 3019 3755 5159
Size: 648 1047 135 342 96 233 736 1404
Cuts at: 5159 5518 5677 518
Size: 359 159 2042
Fragments arranged by size:
2042 1404 1047 736 648 359 342 233
159 135 96
MspA1I CmG'CkG
Cuts at: 1606 2974 3578 4617 5294 6442 6687 1606
Size: 1368 604 1039 677 1148 245 2120
Fragments arranged by size:
2120 1368 1148 1039 677 604 245
NcoI C'CATG_G
Cuts at: 513 1065 3171 513
Size: 552 2106 4543
Fragments arranged by size:
4543 2106 552
NdeI CA'TA_TG
Cuts at: 387 2920 387
Size: 2533 4668
NheI G'CTAG_C
Cuts at: 1052 4003 1052
Size: 2951 4250
NotI GC'GGCC_GC
Cuts at: 4648 4648
Size: 7201
NsiI A_TGCA'T
Cuts at: 2920 2920
Size: 7201
NspI r_CATG'y
Cuts at: 1132 1229 3024 4559 6104 1132
Size: 97 1795 1535 1545 2229
Fragments arranged by size:
2229 1795 1545 1535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7201
PciI A'CATG_T
Cuts at: 3020 6100 3020
Size: 3080 4121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3449 1108
Size: 2341 4860
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 7201
PmeI GTTT'AAAC
Cuts at: 4505 4505
Size: 7201
PmlI CAC'GTG
Cuts at: 3847 3847
Size: 7201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5270 5302 6783 6815 5270
Size: 32 1481 32 5656
Fragments arranged by size:
5656 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3084 4103 3084
Size: 1019 6182
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7201
PsiI TTA'TAA
Cuts at: 4771 4771
Size: 7201
PstI C_TGCA'G
Cuts at: 830 4301 4553 830
Size: 3471 252 3478
Fragments arranged by size:
3478 3471 252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7201
PvuII CAG'CTG
Cuts at: 3578 3578
Size: 7201
SacI G_AGCT'C
Cuts at: 3950 3950
Size: 7201
SalI G'TCGA_C
Cuts at: 1936 4543 1936
Size: 2607 4594
SanDI GG'GwC_CC
Cuts at: 3084 4103 3084
Size: 1019 6182
SbfI CC_TGCA'GG
Cuts at: 4553 4553
Size: 7201
ScaI AGT'ACT
Cuts at: 5449 7161 5449
Size: 1712 5489
SfcI C'TryA_G
Cuts at: 826 1045 3139 3744 4297 4549 5684 6365 6556
Size: 219 2094 605 553 252 1135 681 191
Cuts at: 6556 826
Size: 1471
Fragments arranged by size:
2094 1471 1135 681 605 553 252 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7201
SmaI CCC'GGG
Cuts at: 1872 4528 1872
Size: 2656 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4470 5305 6206 6468 820
Size: 197 926 2000 527 835 901 262 1553
Fragments arranged by size:
2000 1553 926 901 835 527 262 197
SnaBI TAC'GTA
Cuts at: 493 4992 493
Size: 4499 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7201
SphI G_CATG'C
Cuts at: 1132 4559 1132
Size: 3427 3774
SspI AAT'ATT
Cuts at: 5 52 2716 2816 5125 5
Size: 47 2664 100 2309 2081
Fragments arranged by size:
2664 2309 2081 100 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7201
StyI C'CwwG_G
Cuts at: 513 1065 2253 2895 3171 3774 4008 4263 4640
Size: 552 1188 642 276 603 234 255 377
Cuts at: 4640 513
Size: 3074
Fragments arranged by size:
3074 1188 642 603 552 377 276 255
234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2916 5228 5245 5398 5583 6028 325
Size: 1255 1336 2312 17 153 185 445 1498
Fragments arranged by size:
2312 1498 1336 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 3255 3636
Size: 274 80 33 51 1443 561 717 381
Cuts at: 3636 5447 7159 96
Size: 1811 1712 138
Fragments arranged by size:
1811 1712 1443 717 561 381 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2071 2103 2304 2336 7161 7193 2071
Size: 32 201 32 4825 32 2079
Fragments arranged by size:
4825 2079 201 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7201
XbaI T'CTAG_A
Cuts at: 4537 4537
Size: 7201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3169 3200 4360 3169
Size: 31 1160 6010
Fragments arranged by size:
6010 1160 31
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7201
XmaI C'CCGG_G
Cuts at: 1870 4526 1870
Size: 2656 4545
XmnI GAAnn'nnTTC
Cuts at: 2672 5330 2672
Size: 2658 4543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5009 276
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI