Restriction map for
pFN21AE5734
(Circular) (Six-base) MAPSORT of: pFN21AE5734.seq Check: 7522 from: 1 to: 6685
pFN21AE5734 6685 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2557 4028 1937
Size: 620 1471 4594
Fragments arranged by size:
4594 1471 620
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2623 3113 4748 6132 6400 1632
Size: 991 490 1635 1384 268 1917
Fragments arranged by size:
1917 1635 1384 991 490 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 2280 5584 6561 1203
Size: 1077 3304 977 1327
Fragments arranged by size:
3304 1327 1077 977
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 3874 5414 3874
Size: 1540 5145
AleI CACnn'nnGTG
Cuts at: 1166 3665 1166
Size: 2499 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6685
AlwNI CAG_nnn'CTG
Cuts at: 2629 4428 6000 2629
Size: 1799 1572 3314
Fragments arranged by size:
3314 1799 1572
ApaLI G'TGCA_C
Cuts at: 4742 5898 4742
Size: 1156 5529
ApoI r'AATT_y
Cuts at: 1493 2122 2546 3636 3994 4220 4372 6481 1493
Size: 629 424 1090 358 226 152 2109 1697
Fragments arranged by size:
2109 1697 1090 629 424 358 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3110 5239 160
Size: 872 2078 2129 1606
Fragments arranged by size:
2129 2078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 4010 1870
Size: 73 2067 4545
Fragments arranged by size:
4545 2067 73
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2649 4006 5461 618
Size: 325 217 627 862 1357 1455 1842
Fragments arranged by size:
1842 1455 1357 862 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3388 3690 3785 4004 1391
Size: 1997 302 95 219 4072
Fragments arranged by size:
4072 1997 302 219 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2790 928
Size: 971 891 4823
Fragments arranged by size:
4823 971 891
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 3436 1548
Size: 1888 4797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5296 136
Size: 107 122 71 631 605 3624 1525
Fragments arranged by size:
3624 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3850 6680 3850
Size: 2830 3855
BlpI GC'TnA_GC
Cuts at: 2447 4102 2447
Size: 1655 5030
Bme1580I G_kGCm'C
Cuts at: 1790 2647 4746 5902 1790
Size: 857 2099 1156 2573
Fragments arranged by size:
2573 2099 1156 857
BmgBI CAC'GTC
Cuts at: 1318 2281 1318
Size: 963 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4416 5374 442
Size: 416 53 83 399 24 2999 958 1753
Fragments arranged by size:
2999 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 2936 3177 5345 1239
Size: 1418 279 241 2168 2579
Fragments arranged by size:
2579 2168 1418 279 241
Bpu10I CC'TnA_GC
Cuts at: 3042 3042
Size: 6685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3338 4810 5675 5973 3338
Size: 1472 865 298 4050
Fragments arranged by size:
4050 1472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3909 5348 882
Size: 617 2410 1439 2219
Fragments arranged by size:
2410 2219 1439 617
BsaAI yAC'GTr
Cuts at: 493 1204 4476 493
Size: 711 3272 2702
Fragments arranged by size:
3272 2702 711
BsaBI GATnn'nnATC
Cuts at: 3849 6679 3849
Size: 2830 3855
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4492 4874 5509 275
Size: 53 83 186 936 2959 382 635 1451
Fragments arranged by size:
2959 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2201 2550 3084 5118 5516 5790 5937 6348 2201
Size: 349 534 2034 398 274 147 411 2538
Fragments arranged by size:
2538 2034 534 411 398 349 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 5245
Fragments arranged by size:
5245 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 1186
Size: 1153 5532
BseYI C'CCAG_C
Cuts at: 2382 3027 3689 5888 6384 2382
Size: 645 662 2199 496 2683
Fragments arranged by size:
2683 2199 662 645 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 3053 4746 4831 5902 2013
Size: 1040 1693 85 1071 2796
Fragments arranged by size:
2796 1693 1071 1040 85
BsmI GAATG_Cn'
Cuts at: 4196 4289 4196
Size: 93 6592
BsmBI CGTCTCn'nnnn_
Cuts at: 3337 3337
Size: 6685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2647 3053 3388 3690 3785 4004
Size: 399 223 634 406 335 302 95 219
Cuts at: 4004 4746 4831 5902 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 634 406 399 335 302
223 219 95 85
BspEI T'CCGG_A
Cuts at: 2550 6348 2550
Size: 3798 2887
BspHI T'CATG_A
Cuts at: 2838 4574 6304 2838
Size: 1736 1730 3219
Fragments arranged by size:
3219 1736 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4040 844
Size: 3196 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3041 4572 1394
Size: 429 1218 1531 3507
Fragments arranged by size:
3507 1531 1218 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2751 2970 5180 5354 65
Size: 1183 1503 219 2210 174 1396
Fragments arranged by size:
2210 1503 1396 1183 219 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5329 5516 1700
Size: 110 3519 187 2869
Fragments arranged by size:
3519 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 3492 96
Size: 2187 1209 3289
Fragments arranged by size:
3289 2187 1209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 4745 5757 4745
Size: 1012 5673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2756 2860 794
Size: 1962 104 4619
Fragments arranged by size:
4619 1962 104
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 3173 1184
Size: 1989 4696
BstYI r'GATC_y
Cuts at: 1972 3844 3850 4015 4382 4767 4784 6236 6322
Size: 1872 6 165 367 385 17 1452 86
Cuts at: 6322 6334 6680 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1872 1452 385 367 346 165 86
17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2259 2259
Size: 6685
BtgI C'CryG_G
Cuts at: 513 1065 2004 2517 513
Size: 552 939 513 4681
Fragments arranged by size:
4681 939 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2466 3810 487
Size: 683 56 1240 1344 3362
Fragments arranged by size:
3362 1344 1240 683 56
BtsI GCAGTG_nn'
Cuts at: 2988 3579 3978 4201 4995 5015 2988
Size: 591 399 223 794 20 4658
Fragments arranged by size:
4658 794 591 399 223 20
ClaI AT'CG_AT
Cuts at: 1554 2394 1554
Size: 840 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6650
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DraIII CAC_nnn'GTG
Cuts at: 2281 3932 2281
Size: 1651 5034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 5692 809
Size: 507 1155 3221 1802
Fragments arranged by size:
3221 1802 1155 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2514 4132 5021
Size: 54 1425 50 275 18 684 1618 889
Cuts at: 5021 6650 8
Size: 1629 43
Fragments arranged by size:
1629 1618 1425 889 684 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3665 4615 1353
Size: 2312 950 3423
Fragments arranged by size:
3423 2312 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5272 5646 5792 387
Size: 1064 138 3683 374 146 1280
Fragments arranged by size:
3683 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2623 2657 2936 3113 3177 4748 5345
Size: 393 991 34 279 177 64 1571 597
Cuts at: 5345 6132 6400 1239
Size: 787 268 1524
Fragments arranged by size:
1571 1524 991 787 597 393 279 268
177 64 34
EcoNI CCTnn'n_nnAGG
Cuts at: 3789 3789
Size: 6685
EcoO109I rG'GnC_Cy
Cuts at: 3613 3613
Size: 6685
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
FspI TGC'GCA
Cuts at: 5191 5191
Size: 6685
HaeII r_GCGC'y
Cuts at: 1678 5832 1678
Size: 4154 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2556 2588 2833
Size: 32 135 32 470 32 482 32 245
Cuts at: 2833 2865 3336 3368 3860 3892 5326 5358 5400
Size: 32 471 32 492 32 1434 32 42
Cuts at: 5400 5432 1373
Size: 32 2626
Fragments arranged by size:
2626 1434 492 482 471 470 245 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2722 4029 4275 669
Size: 988 281 784 1307 246 3079
Fragments arranged by size:
3079 1307 988 784 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5799 5983 1305
Size: 4494 184 2007
Fragments arranged by size:
4494 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4863
Fragments arranged by size:
4863 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2417 2531 3156 3665 4643 5002
Size: 648 948 303 114 625 509 978 359
Cuts at: 5002 5161 518
Size: 159 2042
Fragments arranged by size:
2042 978 948 648 625 509 359 303
159 114
MspA1I CmG'CkG
Cuts at: 1606 4101 4778 5926 6171 1606
Size: 2495 677 1148 245 2120
Fragments arranged by size:
2495 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5194
Fragments arranged by size:
5194 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 3926 4043 5588 1132
Size: 97 2697 117 1545 2229
Fragments arranged by size:
2697 2229 1545 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6685
PciI A'CATG_T
Cuts at: 5584 5584
Size: 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3914 1108
Size: 2806 3879
PfoI T'CCnGG_A
Cuts at: 2695 3776 2695
Size: 1081 5604
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 2589 4255 2589
Size: 1666 5019
PstI C_TGCA'G
Cuts at: 830 2764 2856 4037 830
Size: 1934 92 1181 3478
Fragments arranged by size:
3478 1934 1181 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SfcI C'TryA_G
Cuts at: 826 1045 2467 2760 2852 4033 5168 5849 6040
Size: 219 1422 293 92 1181 1135 681 191
Cuts at: 6040 826
Size: 1471
Fragments arranged by size:
1471 1422 1181 1135 681 293 219 191
92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3353 4789 5690 5952 820
Size: 197 926 1410 1436 901 262 1553
Fragments arranged by size:
1553 1436 1410 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 3926 4043 1132
Size: 2794 117 3774
Fragments arranged by size:
3774 2794 117
SspI AAT'ATT
Cuts at: 5 52 4609 5
Size: 47 4557 2081
Fragments arranged by size:
4557 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 4124 513
Size: 552 939 21 2099 3074
Fragments arranged by size:
3074 2099 939 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 3891 4712 4729 4882 5067 5512
Size: 1255 794 1517 821 17 153 185 445
Cuts at: 5512 325
Size: 1498
Fragments arranged by size:
1517 1498 1255 821 794 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3492 4931
Size: 274 80 33 51 1443 306 1209 1439
Cuts at: 4931 6643 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1439 1209 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 6645 6677 3284
Size: 32 3329 32 3292
Fragments arranged by size:
3329 3292 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 3166 4021 3166
Size: 855 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3749 3749
Size: 6685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 3098 3240 3636 4814 3098
Size: 142 396 1178 4969
Fragments arranged by size:
4969 1178 396 142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI