Restriction map for
pFN21AE5731
(Circular) (Six-base) MAPSORT of: pFN21AE5731.seq Check: 3436 from: 1 to: 6484
pFN21AE5731 6484 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3771 4294 278
Size: 53 83 186 936 2235 523 2468
Fragments arranged by size:
2468 2235 936 523 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2553 3527 3567 3771 3827 1937
Size: 616 974 40 204 56 4594
Fragments arranged by size:
4594 974 616 204 56 40
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 3585 4611 4984 1563
Size: 2022 1026 373 3063
Fragments arranged by size:
3063 2022 1026 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2247 3039 5383 6360 1203
Size: 1044 792 2344 977 1327
Fragments arranged by size:
2344 1327 1044 977 792
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2663 1184
Size: 1479 5005
AlwNI CAG_nnn'CTG
Cuts at: 3509 4227 5799 3509
Size: 718 1572 4194
Fragments arranged by size:
4194 1572 718
ApaI G_GGCC'C
Cuts at: 2198 3105 2198
Size: 907 5577
ApaLI G'TGCA_C
Cuts at: 2861 4541 5697 2861
Size: 1680 1156 3648
Fragments arranged by size:
3648 1680 1156
ApoI r'AATT_y
Cuts at: 1493 3286 3594 3793 4019 4171 6280 1493
Size: 1793 308 199 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 308 226 199 152
AscI GG'CGCG_CC
Cuts at: 2088 2088
Size: 6484
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2416 3197 3809 1870
Size: 73 129 344 781 612 4545
Fragments arranged by size:
4545 781 612 344 129 73
AvrII C'CTAG_G
Cuts at: 3182 3182
Size: 6484
BamHI G'GATC_C
Cuts at: 2475 3814 4181 2475
Size: 1339 367 4778
Fragments arranged by size:
4778 1339 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2266 3280 3805 5260
Size: 325 217 627 281 198 1014 525 1455
Cuts at: 5260 618
Size: 1842
Fragments arranged by size:
1842 1455 1014 627 525 325 281 217
198
BanII G_rGCy'C
Cuts at: 1391 2198 2397 2930 3105 3175 3358 3803 1391
Size: 807 199 533 175 70 183 445 4072
Fragments arranged by size:
4072 807 533 445 199 183 175 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 928
Size: 971 316 5197
Fragments arranged by size:
5197 971 316
BbvCI CC'TCA_GC
Cuts at: 3015 3015
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4376 5586 3701
Size: 675 1210 4599
Fragments arranged by size:
4599 1210 675
BclI T'GATC_A
Cuts at: 1548 3432 1548
Size: 1884 4600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2430 2564 3353
Size: 107 122 71 631 605 758 134 789
Cuts at: 3353 5095 136
Size: 1742 1525
Fragments arranged by size:
1742 1525 789 758 631 605 134 122
107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2198 2271 2865 3105 4545 5701 1790
Size: 283 125 73 594 240 1440 1156 2573
Fragments arranged by size:
2573 1440 1156 594 283 240 125 73
BmgBI CAC'GTC
Cuts at: 1318 2248 2545 2713 2725 1318
Size: 930 297 168 12 5077
Fragments arranged by size:
5077 930 297 168 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 3305 4215
Size: 416 53 83 399 24 1284 604 910
Cuts at: 4215 5173 442
Size: 958 1753
Fragments arranged by size:
1753 1284 958 910 604 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 2942 2957 3366 3546 3627 5144 1239
Size: 1289 414 15 409 180 81 1517 2579
Fragments arranged by size:
2579 1517 1289 414 409 180 81 15
Bpu10I CC'TnA_GC
Cuts at: 3015 3015
Size: 6484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2726 2765 5147 882
Size: 617 1227 39 2382 2219
Fragments arranged by size:
2382 2219 1227 617 39
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3040 3628 4275 493
Size: 711 1548 288 588 647 2702
Fragments arranged by size:
2702 1548 711 647 588 288
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3530 3768 4291 4673
Size: 53 83 186 936 1997 238 523 382
Cuts at: 4673 5308 275
Size: 635 1451
Fragments arranged by size:
1997 1451 936 635 523 382 238 186
83 53
BsaWI w'CCGG_w
Cuts at: 4917 5315 5589 5736 6147 4917
Size: 398 274 147 411 5254
Fragments arranged by size:
5254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2915 2945 3029 3059 3562
Size: 30 165 30 1512 30 84 30 503
Cuts at: 3562 3592 1178
Size: 30 4070
Fragments arranged by size:
4070 1512 503 165 84 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 1186
Size: 1339 5145
BseYI C'CCAG_C
Cuts at: 2187 3032 3460 5687 6183 2187
Size: 845 428 2227 496 2488
Fragments arranged by size:
2488 2227 845 496 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3177 3470 1163
Size: 573 1441 293 4177
Fragments arranged by size:
4177 1441 573 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2568 3492 3660 3693 3934
Size: 275 186 48 519 924 168 33 241
Cuts at: 3934 4695 5723 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 924 761 519 275 241 186
168 48 33
BsiHKAI G_wGCw'C
Cuts at: 2397 2865 3429 4545 4630 5701 2397
Size: 468 564 1116 85 1071 3180
Fragments arranged by size:
3180 1116 1071 564 468 85
BsmI GAATG_Cn'
Cuts at: 3995 4088 3995
Size: 93 6391
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2198 2271 2397 2865 2930 3105
Size: 399 283 125 73 126 468 65 175
Cuts at: 3105 3175 3358 3429 3803 4545 4630 5701 1391
Size: 70 183 71 374 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 374 283 183
175 126 125 85 73 71 70 65
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 2310 2763 3238 4373 6103 2310
Size: 453 475 1135 1730 2691
Fragments arranged by size:
2691 1730 1135 475 453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3839 844
Size: 2995 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3441 4979 5153 65
Size: 1183 2193 1538 174 1396
Fragments arranged by size:
2193 1538 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5128 5315 1700
Size: 110 3318 187 2869
Fragments arranged by size:
3318 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 2088 2380 1925
Size: 163 292 6029
Fragments arranged by size:
6029 292 163
BssSI C'ACGA_G
Cuts at: 2369 2929 4544 5556 2369
Size: 560 1615 1012 3297
Fragments arranged by size:
3297 1615 1012 560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6484
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 2524 1184
Size: 1340 5144
BstXI CCAn_nnnn'nTGG
Cuts at: 2959 2959
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2235 2409 2475 2742 2841 3609 3814 4181
Size: 263 174 66 267 99 768 205 367
Cuts at: 4181 4566 4583 6035 6121 6133 6479 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 768 385 367 346 267 263
205 174 99 86 66 17 12
BstZ17I GTA'TAC
Cuts at: 3568 3568
Size: 6484
Bsu36I CC'TnA_GG
Cuts at: 3726 3726
Size: 6484
BtgI C'CryG_G
Cuts at: 513 1065 2283 2335 2584 2628 2685 2958 3217
Size: 552 1218 52 249 44 57 273 259
Cuts at: 3217 3486 3687 513
Size: 269 201 3310
Fragments arranged by size:
3310 1218 552 273 269 259 249 201
57 52 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2105 2391 4000 4794 4814 2044
Size: 61 286 1609 794 20 3714
Fragments arranged by size:
3714 1609 794 286 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 3421 3421
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2549 5491 809
Size: 507 1233 2942 1802
Fragments arranged by size:
2942 1802 1233 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2061 2565
Size: 54 1425 50 275 18 216 15 504
Cuts at: 2565 2653 2803 2949 3214 3489 3690 3931 4820
Size: 88 150 146 265 275 201 241 889
Cuts at: 4820 6449 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 504 275 275 265 241
216 201 150 146 88 54 50 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2565 3489 3690 3931 1537
Size: 275 234 519 924 201 241 4090
Fragments arranged by size:
4090 924 519 275 241 234 201
EarI CTCTTCn'nnn_
Cuts at: 1353 2468 2789 4414 1353
Size: 1115 321 1625 3423
Fragments arranged by size:
3423 1625 1115 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2402 5071 5445 5591 387
Size: 1064 138 813 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 813 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2395
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2942 2957 3366 3546 3627 4547
Size: 393 896 414 15 409 180 81 920
Cuts at: 4547 5144 5931 6199 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 920 896 787 597 414 409 393
268 180 81 15
EcoO109I rG'GnC_Cy
Cuts at: 2055 2194 3179 3754 2055
Size: 139 985 575 4785
Fragments arranged by size:
4785 985 575 139
EcoRI G'AATT_C
Cuts at: 3594 3793 4171 3594
Size: 199 378 5907
Fragments arranged by size:
5907 378 199
EcoRV GAT'ATC
Cuts at: 3236 3236
Size: 6484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 6484
FspI TGC'GCA
Cuts at: 2756 4990 2756
Size: 2234 4250
HaeII r_GCGC'y
Cuts at: 1678 2117 5631 1678
Size: 439 3514 2531
Fragments arranged by size:
3514 2531 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5125 5157 5199 5231 1373
Size: 32 135 32 3553 32 42 32 2626
Fragments arranged by size:
3553 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3307 3528 3828 4074 669
Size: 988 281 1369 221 300 246 3079
Fragments arranged by size:
3079 1369 988 300 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5598 5782 1305
Size: 4293 184 2007
Fragments arranged by size:
4293 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2951 10
Size: 54 1425 343 1119 3543
Fragments arranged by size:
3543 1425 1119 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3446 4442 4801 4960 518
Size: 648 2280 996 359 159 2042
Fragments arranged by size:
2280 2042 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2337 2586 2665 3161 3488 3509 3689 3900
Size: 731 249 79 496 327 21 180 211
Cuts at: 3900 4577 5725 5970 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 731 677 496 327 249 245
211 180 79 21
NcoI C'CATG_G
Cuts at: 513 1065 2628 513
Size: 552 1563 4369
Fragments arranged by size:
4369 1563 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3489 3690 3931 3489
Size: 201 241 6042
Fragments arranged by size:
6042 241 201
NspI r_CATG'y
Cuts at: 1132 1229 2894 3842 5387 1132
Size: 97 1665 948 1545 2229
Fragments arranged by size:
2229 1665 1545 948 97
PasI CC'CwG_GG
Cuts at: 1348 3176 1348
Size: 1828 4656
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3421 1108
Size: 2313 4171
PfoI T'CCnGG_A
Cuts at: 2505 2505
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 3754 2055
Size: 1699 4785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2194 3101 2194
Size: 907 5577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2324 2356 2324
Size: 32 6452
PstI C_TGCA'G
Cuts at: 830 2444 3836 830
Size: 1614 1392 3478
Fragments arranged by size:
3478 1614 1392
PvuI CG_AT'CG
Cuts at: 2001 3660 2001
Size: 1659 4825
PvuII CAG'CTG
Cuts at: 2665 3161 2665
Size: 496 5988
SacI G_AGCT'C
Cuts at: 2397 2397
Size: 6484
SacII CC_GC'GG
Cuts at: 2338 2587 3489 3690 2338
Size: 249 902 201 5132
Fragments arranged by size:
5132 902 249 201
SalI G'TCGA_C
Cuts at: 1936 3526 3826 1936
Size: 1590 300 4594
Fragments arranged by size:
4594 1590 300
SapI GCTCTTCn'nnn_
Cuts at: 2789 2789
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 2975 4732 6444 2975
Size: 1757 1712 3015
Fragments arranged by size:
3015 1757 1712
SexAI A'CCwGG_T
Cuts at: 3325 3325
Size: 6484
SfcI C'TryA_G
Cuts at: 826 1045 2134 2440 3648 3668 3832 4967 5648
Size: 219 1089 306 1208 20 164 1135 681
Cuts at: 5648 5839 826
Size: 191 1471
Fragments arranged by size:
1471 1208 1135 1089 681 306 219 191
164 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 2418 3811 1872
Size: 546 1393 4545
Fragments arranged by size:
4545 1393 546
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4588 5489 5751 820
Size: 197 926 2645 901 262 1553
Fragments arranged by size:
2645 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 2894 3842 1132
Size: 1762 948 3774
Fragments arranged by size:
3774 1762 948
SrfI GCCC'GGGC
Cuts at: 2418 2418
Size: 6484
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 3731 1517
Size: 2214 4270
StyI C'CwwG_G
Cuts at: 513 1065 2064 2520 2628 3182 3923 513
Size: 552 999 456 108 554 741 3074
Fragments arranged by size:
3074 999 741 554 552 456 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4511 4528 4681 4866 5311 325
Size: 1255 2931 17 153 185 445 1498
Fragments arranged by size:
2931 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 2973 3042
Size: 274 80 33 51 1443 378 618 69
Cuts at: 3042 4730 6442 96
Size: 1688 1712 138
Fragments arranged by size:
1712 1688 1443 618 378 274 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2915 2947 3525 3557 3562 3594 6444 6476 2915
Size: 32 578 32 5 32 2850 32 2923
Fragments arranged by size:
2923 2850 578 32 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2184 2184
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 2416 3809 1870
Size: 546 1393 4545
Fragments arranged by size:
4545 1393 546
XmnI GAAnn'nnTTC
Cuts at: 2798 4613 2798
Size: 1815 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3769 4292 276
Size: 53 83 186 936 2235 523 2468
Fragments arranged by size:
2468 2235 936 523 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI BaeI BbeI BfrBI BsiWI BsmBI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI RsrII SanDI SfiI SfoI
SwaI