Restriction map for
pFN21AE5729
(Circular) (Six-base) MAPSORT of: pFN21AE5729.seq Check: 8645 from: 1 to: 6625
pFN21AE5729 6625 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3032 3894 4435 278
Size: 53 83 186 936 1496 862 541 2468
Fragments arranged by size:
2468 1496 936 862 541 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2360 2909 3041 4688 6072 6340 1632
Size: 416 312 549 132 1647 1384 268 1917
Fragments arranged by size:
1917 1647 1384 549 416 312 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2064 5524 6501 1203
Size: 861 3460 977 1327
Fragments arranged by size:
3460 1327 977 861
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6625
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 6593
AlwNI CAG_nnn'CTG
Cuts at: 3832 3886 4368 5940 3832
Size: 54 482 1572 4517
Fragments arranged by size:
4517 1572 482 54
ApaI G_GGCC'C
Cuts at: 2057 2142 3277 3813 2057
Size: 85 1135 536 4869
Fragments arranged by size:
4869 1135 536 85
ApaLI G'TGCA_C
Cuts at: 3085 4682 5838 3085
Size: 1597 1156 3872
Fragments arranged by size:
3872 1597 1156
ApoI r'AATT_y
Cuts at: 1493 2879 3506 3934 4160 4312 6421 1493
Size: 1386 627 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 627 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 3950 1870
Size: 73 2007 4545
Fragments arranged by size:
4545 2007 73
BamHI G'GATC_C
Cuts at: 3955 4322 3955
Size: 367 6258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 3845 3946 5401 618
Size: 325 217 627 905 1153 101 1455 1842
Fragments arranged by size:
1842 1455 1153 905 627 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2057 2142 2769 3075 3277 3602 3706 3813
Size: 666 85 627 306 202 325 104 107
Cuts at: 3813 3944 1391
Size: 131 4072
Fragments arranged by size:
4072 666 627 325 306 202 131 107
104 85
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 3155 928
Size: 971 1023 233 4398
Fragments arranged by size:
4398 1023 971 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2841 2875 4816 4850 1903
Size: 34 50 34 820 34 1941 34 3678
Fragments arranged by size:
3678 1941 820 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3582 5236 136
Size: 107 122 71 631 605 1910 1654 1525
Fragments arranged by size:
1910 1654 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 3289 6620 2550
Size: 739 3331 2555
Fragments arranged by size:
3331 2555 739
BlpI GC'TnA_GC
Cuts at: 2396 2536 2763 4042 2396
Size: 140 227 1279 4979
Fragments arranged by size:
4979 1279 227 140
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2142 3089 3277 3813 4686 5842 1790
Size: 267 85 947 188 536 873 1156 2573
Fragments arranged by size:
2573 1156 947 873 536 267 188 85
BmgBI CAC'GTC
Cuts at: 1318 2790 3424 1318
Size: 1472 634 4519
Fragments arranged by size:
4519 1472 634
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2602 2842 3280
Size: 416 53 83 399 24 1185 240 438
Cuts at: 3280 3352 3813 3816 4356 5314 442
Size: 72 461 3 540 958 1753
Fragments arranged by size:
1753 1185 958 540 461 438 416 399
240 83 72 53 24 3
BmtI G_CTAG'C
Cuts at: 1056 2307 1056
Size: 1251 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 5285 1239
Size: 1154 2892 2579
Fragments arranged by size:
2892 2579 1154
Bpu10I CC'TnA_GC
Cuts at: 2391 3464 2391
Size: 1073 5552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2248 4750 5615 5913 2248
Size: 2502 865 298 2960
Fragments arranged by size:
2960 2502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 5288 882
Size: 617 1812 1977 2219
Fragments arranged by size:
2219 1977 1812 617
BsaAI yAC'GTr
Cuts at: 493 1204 2011 2067 4416 493
Size: 711 807 56 2349 2702
Fragments arranged by size:
2702 2349 807 711 56
BsaBI GATnn'nnATC
Cuts at: 3371 3635 3656 6619 3371
Size: 264 21 2963 3377
Fragments arranged by size:
3377 2963 264 21
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3029 3891 4432 4814
Size: 53 83 186 936 1496 862 541 382
Cuts at: 4814 5449 275
Size: 635 1451
Fragments arranged by size:
1496 1451 936 862 635 541 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2619 2778 5058 5456 5730 5877 6288 2619
Size: 159 2280 398 274 147 411 2956
Fragments arranged by size:
2956 2280 411 398 274 159 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3851 3881 1178
Size: 30 165 30 2448 30 3922
Fragments arranged by size:
3922 2448 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6625
BseYI C'CCAG_C
Cuts at: 2232 5828 6324 2232
Size: 3596 496 2533
Fragments arranged by size:
3596 2533 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6052
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2946 4075 4836 5864 1540
Size: 275 186 945 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 945 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3089 4686 4771 5842 3089
Size: 1597 85 1071 3872
Fragments arranged by size:
3872 1597 1071 85
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
BsmBI CGTCTCn'nnnn_
Cuts at: 2251 3020 2251
Size: 769 5856
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2142 2769 3075 3089 3277 3602
Size: 399 267 85 627 306 14 188 325
Cuts at: 3602 3706 3813 3944 4686 4771 5842 1391
Size: 104 107 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 399 325 306 267
188 131 107 104 85 85 14
BspEI T'CCGG_A
Cuts at: 2619 6288 2619
Size: 3669 2956
BspHI T'CATG_A
Cuts at: 4514 6244 4514
Size: 1730 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 3329 3980 844
Size: 1187 1298 651 3489
Fragments arranged by size:
3489 1298 1187 651
BsrBI CCG'CTC
Cuts at: 1394 1823 3439 4512 1394
Size: 429 1616 1073 3507
Fragments arranged by size:
3507 1616 1073 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2305 3277 3355 5120 5294 65
Size: 1183 1057 972 78 1765 174 1396
Fragments arranged by size:
1765 1396 1183 1057 972 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 5269 5456 1700
Size: 110 3459 187 2869
Fragments arranged by size:
3459 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2966 3044 4685 5697 2966
Size: 78 1641 1012 3894
Fragments arranged by size:
3894 1641 1012 78
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2044 3832 794
Size: 1250 1788 3587
Fragments arranged by size:
3587 1788 1250
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 3379 3379
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2550 3289 3955 4322 4707 4724 6176 6262
Size: 578 739 666 367 385 17 1452 86
Cuts at: 6262 6274 6620 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 739 666 578 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2110 2271 2487 2563 2940 3232 3378
Size: 552 1045 161 216 76 377 292 146
Cuts at: 3378 3735 513
Size: 357 3403
Fragments arranged by size:
3403 1045 552 377 357 292 216 161
146 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5886
Fragments arranged by size:
5886 683 56
BtsI GCAGTG_nn'
Cuts at: 2207 3224 4141 4935 4955 2207
Size: 1017 917 794 20 3877
Fragments arranged by size:
3877 1017 917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3028 5632 809
Size: 507 1712 2604 1802
Fragments arranged by size:
2604 1802 1712 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2577 2862 2943
Size: 54 1425 50 275 18 747 285 81
Cuts at: 2943 3208 3574 3678 3738 4072 4961 6590 8
Size: 265 366 104 60 334 889 1629 43
Fragments arranged by size:
1629 1425 889 747 366 334 285 275
265 104 81 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2943 4072 1537
Size: 275 1131 1129 4090
Fragments arranged by size:
4090 1131 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 4555 1353
Size: 830 2372 3423
Fragments arranged by size:
3423 2372 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5212 5586 5732 387
Size: 1064 138 3623 374 146 1280
Fragments arranged by size:
3623 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2360 2393 2909 3041 4688 5285
Size: 393 416 312 33 516 132 1647 597
Cuts at: 5285 6072 6340 1239
Size: 787 268 1524
Fragments arranged by size:
1647 1524 787 597 516 416 393 312
268 132 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2027
Size: 6625
EcoO109I rG'GnC_Cy
Cuts at: 2032 2053 2054 2138 2220 2344 2604 3106 3810
Size: 21 1 84 82 124 260 502 704
Cuts at: 3810 3829 2032
Size: 19 4828
Fragments arranged by size:
4828 704 502 260 124 84 82 21
19 1
EcoRI G'AATT_C
Cuts at: 2879 3934 4312 2879
Size: 1055 378 5192
Fragments arranged by size:
5192 1055 378
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 5772 1678
Size: 4094 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2960 2992 5266 5298 5340
Size: 32 135 32 1388 32 2274 32 42
Cuts at: 5340 5372 1373
Size: 32 2626
Fragments arranged by size:
2626 2274 1388 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3969 4215 669
Size: 988 281 2031 246 3079
Fragments arranged by size:
3079 2031 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3053 748
Size: 2305 4320
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5739 5923 1305
Size: 4434 184 2007
Fragments arranged by size:
4434 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 3210 3576 3680 10
Size: 54 1425 343 747 631 366 104 2955
Fragments arranged by size:
2955 1425 747 631 366 343 104 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2406 3329 3377 4583 4942 5101
Size: 648 843 397 923 48 1206 359 159
Cuts at: 5101 518
Size: 2042
Fragments arranged by size:
2042 1206 923 843 648 397 359 159
48
MspA1I CmG'CkG
Cuts at: 1606 3697 3886 4041 4718 5866 6111 1606
Size: 2091 189 155 677 1148 245 2120
Fragments arranged by size:
2120 2091 1148 677 245 189 155
NcoI C'CATG_G
Cuts at: 513 1065 2271 2487 3378 513
Size: 552 1206 216 891 3760
Fragments arranged by size:
3760 1206 891 552 216
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 2303 1052
Size: 1251 5374
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 3334 3983 5528 1132
Size: 97 2105 649 1545 2229
Fragments arranged by size:
2229 2105 1545 649 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 5524 5524
Size: 6625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 5245
PfoI T'CCnGG_A
Cuts at: 2370 2898 2370
Size: 528 6097
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PmlI CAC'GTG
Cuts at: 2011 2011
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2344 2604 3106 2220
Size: 124 260 502 5739
Fragments arranged by size:
5739 502 260 124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PspOMI G'GGCC_C
Cuts at: 2053 2138 3273 3809 2053
Size: 85 1135 536 4869
Fragments arranged by size:
4869 1135 536 85
PstI C_TGCA'G
Cuts at: 830 2049 2670 3882 3977 830
Size: 1219 621 1212 95 3478
Fragments arranged by size:
3478 1219 1212 621 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 2183
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 2670 3977 2670
Size: 1307 5318
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SfcI C'TryA_G
Cuts at: 826 1045 2045 2666 3076 3124 3293 3878 3973
Size: 219 1000 621 410 48 169 585 95
Cuts at: 3973 5108 5789 5980 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1000 681 621 585 410 219
191 169 95 48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2227 4729 5630 5892 820
Size: 197 926 284 2502 901 262 1553
Fragments arranged by size:
2502 1553 926 901 284 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3983 1132
Size: 2851 3774
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 3158 3401 1517
Size: 1641 243 4741
Fragments arranged by size:
4741 1641 243
StyI C'CwwG_G
Cuts at: 513 1065 2271 2487 3378 3675 4064 513
Size: 552 1206 216 891 297 389 3074
Fragments arranged by size:
3074 1206 891 552 389 297 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3371 3560 4652 4669 4822 5007
Size: 1255 543 1248 189 1092 17 153 185
Cuts at: 5007 5452 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1248 1092 543 445 189 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 4871 6583
Size: 274 80 33 51 1443 1383 1511 1712
Cuts at: 6583 96
Size: 138
Fragments arranged by size:
1712 1511 1443 1383 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 6585 6617 2439
Size: 32 4114 32 2447
Fragments arranged by size:
4114 2447 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2921 1316
Size: 1605 5020
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3664 3764 3664
Size: 100 6525
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 4754 4754
Size: 6625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3030 3892 4433 276
Size: 53 83 186 936 1496 862 541 2468
Fragments arranged by size:
2468 1496 936 862 541 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI