Restriction map for
pFN21AE5724
(Circular) (Six-base) MAPSORT of: pFN21AE5724.seq Check: 2491 from: 1 to: 7027
pFN21AE5724 7027 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3091 4036 3091
Size: 945 6082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2979 4837 278
Size: 53 83 186 936 1443 1858 2468
Fragments arranged by size:
2468 1858 1443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 4370 1937
Size: 667 1766 4594
Fragments arranged by size:
4594 1766 667
Acc65I G'GTAC_C
Cuts at: 4348 4348
Size: 7027
AclI AA'CG_TT
Cuts at: 1563 5154 5527 1563
Size: 3591 373 3063
Fragments arranged by size:
3591 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3821 5090 6474 6742 1632
Size: 2189 1269 1384 268 1917
Fragments arranged by size:
2189 1917 1384 1269 268
AfeI AGC'GCT
Cuts at: 3677 3677
Size: 7027
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6830
AflIII A'CryG_T
Cuts at: 1203 3114 5926 6903 1203
Size: 1911 2812 977 1327
Fragments arranged by size:
2812 1911 1327 977
AgeI A'CCGG_T
Cuts at: 5858 5858
Size: 7027
AhdI GACnn_n'nnGTC
Cuts at: 5756 5756
Size: 7027
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7027
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7027
AlwNI CAG_nnn'CTG
Cuts at: 2815 3061 3848 4283 4770 6342 2815
Size: 246 787 435 487 1572 3500
Fragments arranged by size:
3500 1572 787 487 435 246
ApaI G_GGCC'C
Cuts at: 2623 3772 3893 2623
Size: 1149 121 5757
Fragments arranged by size:
5757 1149 121
ApaLI G'TGCA_C
Cuts at: 5084 6240 5084
Size: 1156 5871
ApoI r'AATT_y
Cuts at: 1493 3655 4336 4562 4714 6823 1493
Size: 2162 681 226 152 2109 1697
Fragments arranged by size:
2162 2109 1697 681 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5581 160
Size: 872 4549 1606
Fragments arranged by size:
4549 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
AvaI C'yCGr_G
Cuts at: 1870 1943 3444 3750 3772 4056 4125 4299 4352
Size: 73 1501 306 22 284 69 174 53
Cuts at: 4352 1870
Size: 4545
Fragments arranged by size:
4545 1501 306 284 174 73 69 53
22
BamHI G'GATC_C
Cuts at: 3467 4357 4724 3467
Size: 890 367 5770
Fragments arranged by size:
5770 890 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2145 2482 2818 2956
Size: 325 217 627 281 77 337 336 138
Cuts at: 2956 3008 3440 3976 4348 5803 618
Size: 52 432 536 372 1455 1842
Fragments arranged by size:
1842 1455 627 536 432 372 337 336
325 281 217 138 77 52
BanII G_rGCy'C
Cuts at: 1391 2623 3383 3772 3787 3893 4236 4346 1391
Size: 1232 760 389 15 106 343 110 4072
Fragments arranged by size:
4072 1232 760 389 343 110 106 15
BbeI G_GCGC'C
Cuts at: 3444 3444
Size: 7027
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 3340 928
Size: 971 481 960 4615
Fragments arranged by size:
4615 971 960 481
BbvCI CC'TCA_GC
Cuts at: 2598 2660 4164 2598
Size: 62 1504 5461
Fragments arranged by size:
5461 1504 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3175 3209 5218 5252 1903
Size: 34 1238 34 2009 34 3678
Fragments arranged by size:
3678 2009 1238 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4919 6129 4919
Size: 1210 5817
BclI T'GATC_A
Cuts at: 1548 2298 1548
Size: 750 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4270 5638
Size: 107 122 71 631 605 337 2261 1368
Cuts at: 5638 136
Size: 1525
Fragments arranged by size:
2261 1525 1368 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2712 3483 7022 2712
Size: 771 3539 2717
Fragments arranged by size:
3539 2717 771
BlpI GC'TnA_GC
Cuts at: 2993 3221 3786 3949 4444 2993
Size: 228 565 163 495 5576
Fragments arranged by size:
5576 565 495 228 163
Bme1580I G_kGCm'C
Cuts at: 1790 2623 2823 3772 3893 3981 5088 6244 1790
Size: 833 200 949 121 88 1107 1156 2573
Fragments arranged by size:
2573 1156 1107 949 833 200 121 88
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 2803 4758
Size: 416 53 83 399 24 882 504 1955
Cuts at: 4758 5716 442
Size: 958 1753
Fragments arranged by size:
1955 1753 958 882 504 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 7027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2147 2600 3900 3945 4080 4292 5687
Size: 842 66 453 1300 45 135 212 1395
Cuts at: 5687 1239
Size: 2579
Fragments arranged by size:
2579 1395 1300 842 453 212 135 66
45
Bpu10I CC'TnA_GC
Cuts at: 2130 2598 2651 2660 4164 4284 2130
Size: 468 53 9 1504 120 4873
Fragments arranged by size:
4873 1504 468 120 53 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3602 5152 6017 6315 3602
Size: 1550 865 298 4314
Fragments arranged by size:
4314 1550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2860 4081 4310 5690 882
Size: 617 1361 1221 229 1380 2219
Fragments arranged by size:
2219 1380 1361 1221 617 229
BsaAI yAC'GTr
Cuts at: 493 1204 4818 493
Size: 711 3614 2702
Fragments arranged by size:
3614 2702 711
BsaBI GATnn'nnATC
Cuts at: 7021 7021
Size: 7027
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2976 3076 3441 4834
Size: 53 83 186 936 1443 100 365 1393
Cuts at: 4834 5216 5851 275
Size: 382 635 1451
Fragments arranged by size:
1451 1443 1393 936 635 382 365 186
100 83 53
BsaWI w'CCGG_w
Cuts at: 2280 5460 5858 6132 6279 6690 2280
Size: 3180 398 274 147 411 2617
Fragments arranged by size:
3180 2617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 2573 2603 1178
Size: 30 165 30 765 30 375 30 5602
Fragments arranged by size:
5602 765 375 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2155 2378 2474 2723 3254 3599 4116 1186
Size: 969 223 96 249 531 345 517 4097
Fragments arranged by size:
4097 969 531 517 345 249 223 96
BseYI C'CCAG_C
Cuts at: 2850 3305 4084 4260 6230 6726 2850
Size: 455 779 176 1970 496 3151
Fragments arranged by size:
3151 1970 779 496 455 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4025 1163
Size: 573 2289 4165
Fragments arranged by size:
4165 2289 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2024 2097 4477 5238 6266 1540
Size: 275 186 23 73 2380 761 1028 2301
Fragments arranged by size:
2380 2301 1028 761 275 186 73 23
BsiHKAI G_wGCw'C
Cuts at: 2433 3174 3779 3787 5088 5173 6244 2433
Size: 741 605 8 1301 85 1071 3216
Fragments arranged by size:
3216 1301 1071 741 605 85 8
BsmI GAATG_Cn'
Cuts at: 4538 4631 4538
Size: 93 6934
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2433 2623 2823 3174 3383 3772 3779
Size: 399 643 190 200 351 209 389 7
Cuts at: 3779 3787 3893 3981 4236 4346 5088 5173 6244
Size: 8 106 88 255 110 742 85 1071
Cuts at: 6244 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 643 399 389 351 255
209 200 190 110 106 88 85 8
7
BspEI T'CCGG_A
Cuts at: 6690 6690
Size: 7027
BspHI T'CATG_A
Cuts at: 4916 6646 4916
Size: 1730 5297
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3091 4036 4382 844
Size: 1673 574 945 346 3489
Fragments arranged by size:
3489 1673 945 574 346
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 4216 4914 1394
Size: 429 410 1983 698 3507
Fragments arranged by size:
3507 1983 698 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 3867 5522 5696 65
Size: 1183 2619 1655 174 1396
Fragments arranged by size:
2619 1655 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 3504 5671 5858 1700
Size: 110 213 1481 2167 187 2869
Fragments arranged by size:
2869 2167 1481 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7027
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7027
BssSI C'ACGA_G
Cuts at: 2881 3158 3365 5087 6099 2881
Size: 277 207 1722 1012 3809
Fragments arranged by size:
3809 1722 1012 277 207
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3418 3727 794
Size: 2624 309 4094
Fragments arranged by size:
4094 2624 309
BstBI TT'CG_AA
Cuts at: 5831 5831
Size: 7027
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7027
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 3810 2746
Size: 1064 5963
BstYI r'GATC_y
Cuts at: 1972 2422 2472 2646 2712 3361 3467 3483 4357
Size: 450 50 174 66 649 106 16 874
Cuts at: 4357 4724 5109 5126 6578 6664 6676 7022 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 874 649 450 385 367 346
174 106 86 66 50 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 2104 4228 2104
Size: 2124 4903
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 2486 2585 3042 3384 3436
Size: 552 939 93 389 99 457 342 52
Cuts at: 3436 3624 3995 513
Size: 188 371 3545
Fragments arranged by size:
3545 939 552 457 389 371 342 188
99 93 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4194 487
Size: 683 56 2968 3320
Fragments arranged by size:
3320 2968 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 3315 3706 4543 5337 5357 2220
Size: 1095 391 837 794 20 3890
Fragments arranged by size:
3890 1095 837 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3910 3945 529
Size: 35 3346 35 3611
Fragments arranged by size:
3611 3346 35 35
DraI TTT'AAA
Cuts at: 4331 4331
Size: 7027
DraIII CAC_nnn'GTG
Cuts at: 2872 3727 2872
Size: 855 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 2852 6034 809
Size: 507 1284 252 3182 1802
Fragments arranged by size:
3182 1802 1284 507 252
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2736 3034
Size: 54 1425 50 275 18 191 715 298
Cuts at: 3034 3621 3864 4239 4474 5363 6992 8
Size: 587 243 375 235 889 1629 43
Fragments arranged by size:
1629 1425 889 715 587 375 298 275
243 235 191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2021 4474 1537
Size: 275 209 2453 4090
Fragments arranged by size:
4090 2453 275 209
EarI CTCTTCn'nnn_
Cuts at: 1353 2545 3073 4957 1353
Size: 1192 528 1884 3423
Fragments arranged by size:
3423 1884 1192 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2660 5614 5988 6134 387
Size: 1064 138 1071 2954 374 146 1280
Fragments arranged by size:
2954 1280 1071 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3785 3785
Size: 7027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2147 2600 3821 3900 3945 4080
Size: 393 449 66 453 1221 79 45 135
Cuts at: 4080 4292 5090 5687 6474 6742 1239
Size: 212 798 597 787 268 1524
Fragments arranged by size:
1524 1221 798 787 597 453 449 393
268 212 135 79 66 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2912 4098 4227 2912
Size: 1186 129 5712
Fragments arranged by size:
5712 1186 129
EcoO109I rG'GnC_Cy
Cuts at: 2250 2293 2620 3265 3769 3889 3890 4088 4121
Size: 43 327 645 504 120 1 198 33
Cuts at: 4121 2250
Size: 5156
Fragments arranged by size:
5156 645 504 327 198 120 43 33
1
EcoRI G'AATT_C
Cuts at: 3655 4336 4714 3655
Size: 681 378 5968
Fragments arranged by size:
5968 681 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3355 3418 3355
Size: 63 6964
FspI TGC'GCA
Cuts at: 2513 5533 2513
Size: 3020 4007
HaeII r_GCGC'y
Cuts at: 1678 3444 3679 3949 4069 6174 1678
Size: 1766 235 270 120 2105 2531
Fragments arranged by size:
2531 2105 1766 270 235 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 5668 5700 5742
Size: 32 135 32 950 32 3114 32 42
Cuts at: 5742 5774 1373
Size: 32 2626
Fragments arranged by size:
3114 2626 950 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2530 4371 4617 669
Size: 988 281 238 354 1841 246 3079
Fragments arranged by size:
3079 1841 988 354 281 246 238
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7027
HpaI GTT'AAC
Cuts at: 4617 4617
Size: 7027
KasI G'GCGC_C
Cuts at: 3440 3440
Size: 7027
KpnI G_GTAC'C
Cuts at: 4352 4352
Size: 7027
MfeI C'AATT_G
Cuts at: 4626 4626
Size: 7027
MluI A'CGCG_T
Cuts at: 6903 6903
Size: 7027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2571 6141 6325 1305
Size: 1266 3570 184 2007
Fragments arranged by size:
3570 2007 1266 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 3623 3866 4241 10
Size: 54 1425 343 906 885 243 375 2796
Fragments arranged by size:
2796 1425 906 885 375 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 2771 2796 3570 4985 5344 5503
Size: 648 1287 318 25 774 1415 359 159
Cuts at: 5503 518
Size: 2042
Fragments arranged by size:
2042 1415 1287 774 648 359 318 159
25
MspA1I CmG'CkG
Cuts at: 1606 2020 2099 2246 2488 3286 3438 3670 3845
Size: 414 79 147 242 798 152 232 175
Cuts at: 3845 3886 3973 4168 4443 5120 6268 6513 1606
Size: 41 87 195 275 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 414 275 245 242
232 195 175 152 147 87 79 41
NaeI GCC'GGC
Cuts at: 2025 3506 2025
Size: 1481 5546
NarI GG'CG_CC
Cuts at: 3441 3441
Size: 7027
NcoI C'CATG_G
Cuts at: 513 1065 2004 3624 3995 513
Size: 552 939 1620 371 3545
Fragments arranged by size:
3545 1620 939 552 371
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7027
NgoMIV G'CCGG_C
Cuts at: 2023 3504 2023
Size: 1481 5546
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7027
NotI GC'GGCC_GC
Cuts at: 4474 4474
Size: 7027
NruI TCG'CGA
Cuts at: 3237 3237
Size: 7027
NspI r_CATG'y
Cuts at: 1132 1229 2932 2971 3018 3118 3283 3830 4385
Size: 97 1703 39 47 100 165 547 555
Cuts at: 4385 5930 1132
Size: 1545 2229
Fragments arranged by size:
2229 1703 1545 555 547 165 100 97
47 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7027
PciI A'CATG_T
Cuts at: 3114 5926 3114
Size: 2812 4215
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3061 1108
Size: 1953 5074
PfoI T'CCnGG_A
Cuts at: 4096 4096
Size: 7027
PmeI GTTT'AAAC
Cuts at: 4331 4331
Size: 7027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 6609 6641 5096
Size: 32 1481 32 5482
Fragments arranged by size:
5482 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 4088 4121 2293
Size: 1795 33 5199
Fragments arranged by size:
5199 1795 33
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7027
PsiI TTA'TAA
Cuts at: 4597 4597
Size: 7027
PspOMI G'GGCC_C
Cuts at: 2619 3768 3889 2619
Size: 1149 121 5757
Fragments arranged by size:
5757 1149 121
PstI C_TGCA'G
Cuts at: 830 2811 2832 3322 3669 4379 830
Size: 1981 21 490 347 710 3478
Fragments arranged by size:
3478 1981 710 490 347 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7027
PvuII CAG'CTG
Cuts at: 3845 3845
Size: 7027
SacI G_AGCT'C
Cuts at: 3787 3787
Size: 7027
SacII CC_GC'GG
Cuts at: 2100 2489 3439 2100
Size: 389 950 5688
Fragments arranged by size:
5688 950 389
SalI G'TCGA_C
Cuts at: 1936 4369 1936
Size: 2433 4594
SanDI GG'GwC_CC
Cuts at: 4088 4088
Size: 7027
SbfI CC_TGCA'GG
Cuts at: 4379 4379
Size: 7027
ScaI AGT'ACT
Cuts at: 3164 3807 5275 6987 3164
Size: 643 1468 1712 3204
Fragments arranged by size:
3204 1712 1468 643
SexAI A'CCwGG_T
Cuts at: 2516 2516
Size: 7027
SfcI C'TryA_G
Cuts at: 826 1045 2269 2807 2828 2961 3318 3665 4375
Size: 219 1224 538 21 133 357 347 710
Cuts at: 4375 5510 6191 6382 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1224 1135 710 681 538 357 347
219 191 133 21
SfoI GGC'GCC
Cuts at: 3442 3442
Size: 7027
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7027
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7027
SmaI CCC'GGG
Cuts at: 1872 3446 4058 4301 4354 1872
Size: 1574 612 243 53 4545
Fragments arranged by size:
4545 1574 612 243 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3581 5131 6032 6294 820
Size: 197 926 1638 1550 901 262 1553
Fragments arranged by size:
1638 1553 1550 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4818 493
Size: 4325 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7027
SphI G_CATG'C
Cuts at: 1132 2971 4385 1132
Size: 1839 1414 3774
Fragments arranged by size:
3774 1839 1414
SspI AAT'ATT
Cuts at: 5 52 4951 5
Size: 47 4899 2081
Fragments arranged by size:
4899 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7027
StyI C'CwwG_G
Cuts at: 513 1065 2004 2149 2378 3624 3995 4250 4466
Size: 552 939 145 229 1246 371 255 216
Cuts at: 4466 513
Size: 3074
Fragments arranged by size:
3074 1246 939 552 371 255 229 216
145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5054 5071 5224 5409 5854 325
Size: 1255 3474 17 153 185 445 1498
Fragments arranged by size:
3474 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3093 3111 3162
Size: 274 80 33 51 1443 1116 18 51
Cuts at: 3162 3399 3805 5273 6985 96
Size: 237 406 1468 1712 138
Fragments arranged by size:
1712 1468 1443 1116 406 274 237 138
80 51 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6987 7019 6987
Size: 32 6995
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7027
XbaI T'CTAG_A
Cuts at: 4363 4363
Size: 7027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2866 2866
Size: 7027
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7027
XmaI C'CCGG_G
Cuts at: 1870 3444 4056 4299 4352 1870
Size: 1574 612 243 53 4545
Fragments arranged by size:
4545 1574 612 243 53
XmnI GAAnn'nnTTC
Cuts at: 5156 5156
Size: 7027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2977 4835 276
Size: 53 83 186 936 1443 1858 2468
Fragments arranged by size:
2468 1858 1443 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI
RsrII SapI SfiI SrfI SwaI