Restriction map for
pFN21AE5723
(Circular) (Six-base) MAPSORT of: pFN21AE5723.seq Check: 4136 from: 1 to: 7147
pFN21AE5723 7147 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4957 278
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4490 1937
Size: 2553 4594
Acc65I G'GTAC_C
Cuts at: 4468 4468
Size: 7147
AclI AA'CG_TT
Cuts at: 1563 5274 5647 1563
Size: 3711 373 3063
Fragments arranged by size:
3711 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 3510 5210 6594 6862 1632
Size: 441 1437 1700 1384 268 1917
Fragments arranged by size:
1917 1700 1437 1384 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6950
AflIII A'CryG_T
Cuts at: 1203 2122 4108 6046 7023 1203
Size: 919 1986 1938 977 1327
Fragments arranged by size:
1986 1938 1327 977 919
AgeI A'CCGG_T
Cuts at: 5978 5978
Size: 7147
AhdI GACnn_n'nnGTC
Cuts at: 5876 5876
Size: 7147
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4162 1184
Size: 2978 4169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4170 4202 4170
Size: 32 7115
AlwNI CAG_nnn'CTG
Cuts at: 3533 4890 6462 3533
Size: 1357 1572 4218
Fragments arranged by size:
4218 1572 1357
ApaLI G'TGCA_C
Cuts at: 4410 5204 6360 4410
Size: 794 1156 5197
Fragments arranged by size:
5197 1156 794
ApoI r'AATT_y
Cuts at: 1493 2560 2584 3433 4456 4682 4834 6943 1493
Size: 1067 24 849 1023 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 1023 849 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 2358 5701 160
Size: 872 1326 3343 1606
Fragments arranged by size:
3343 1606 1326 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
AvaI C'yCGr_G
Cuts at: 1870 1943 4472 1870
Size: 73 2529 4545
Fragments arranged by size:
4545 2529 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3938 3971 3938
Size: 33 7114
BamHI G'GATC_C
Cuts at: 3578 4477 4844 3578
Size: 899 367 5881
Fragments arranged by size:
5881 899 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 4468 5923 618
Size: 325 217 627 1064 1617 1455 1842
Fragments arranged by size:
1842 1617 1455 1064 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2343 4466 1391
Size: 952 2123 4072
Fragments arranged by size:
4072 2123 952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 4082 928
Size: 971 189 1994 3993
Fragments arranged by size:
3993 1994 971 189
BbvCI CC'TCA_GC
Cuts at: 2761 2761
Size: 7147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5338 5372 1903
Size: 34 50 34 3317 34 3678
Fragments arranged by size:
3678 3317 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5039 6249 5039
Size: 1210 5937
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7147
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5758 136
Size: 107 122 71 631 605 4086 1525
Fragments arranged by size:
4086 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7142 7142
Size: 7147
BlpI GC'TnA_GC
Cuts at: 4564 4564
Size: 7147
Bme1580I G_kGCm'C
Cuts at: 1790 4414 5208 6364 1790
Size: 2624 794 1156 2573
Fragments arranged by size:
2624 2573 1156 794
BmgBI CAC'GTC
Cuts at: 1318 3446 1318
Size: 2128 5019
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4878 5836
Size: 416 53 83 399 24 2147 1314 958
Cuts at: 5836 442
Size: 1753
Fragments arranged by size:
2147 1753 1314 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2952 1056
Size: 1896 5251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 3378 3732 4258 5807 1239
Size: 1199 940 354 526 1549 2579
Fragments arranged by size:
2579 1549 1199 940 526 354
Bpu10I CC'TnA_GC
Cuts at: 2761 2761
Size: 7147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 2672 4105 5272 6137 6435 2246
Size: 426 1433 1167 865 298 2958
Fragments arranged by size:
2958 1433 1167 865 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 5810 882
Size: 617 2703 1608 2219
Fragments arranged by size:
2703 2219 1608 617
BsaAI yAC'GTr
Cuts at: 493 1204 4938 493
Size: 711 3734 2702
Fragments arranged by size:
3734 2702 711
BsaBI GATnn'nnATC
Cuts at: 3231 7141 3231
Size: 3910 3237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4954 5336 5971 275
Size: 53 83 186 936 3421 382 635 1451
Fragments arranged by size:
3421 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3581 5580 5978 6252 6399 6810 3581
Size: 1999 398 274 147 411 3918
Fragments arranged by size:
3918 1999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3351 3381 4170 4200 1178
Size: 30 165 30 1948 30 789 30 4125
Fragments arranged by size:
4125 1948 789 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7147
BseYI C'CCAG_C
Cuts at: 6350 6846 6350
Size: 496 6651
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3608 1163
Size: 573 1872 4702
Fragments arranged by size:
4702 1872 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4597 5358 6386 1540
Size: 275 186 2596 761 1028 2301
Fragments arranged by size:
2596 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2369 4405 4414 5208 5293 6364 2369
Size: 2036 9 794 85 1071 3152
Fragments arranged by size:
3152 2036 1071 794 85 9
BsmI GAATG_Cn'
Cuts at: 2952 3292 4171 4658 4751 2952
Size: 340 879 487 93 5348
Fragments arranged by size:
5348 879 487 340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2369 4405 4414 4466 5208 5293
Size: 399 553 26 2036 9 52 742 85
Cuts at: 5293 6364 1391
Size: 1071 2174
Fragments arranged by size:
2174 2036 1071 742 553 399 85 52
26 9
BspEI T'CCGG_A
Cuts at: 6810 6810
Size: 7147
BspHI T'CATG_A
Cuts at: 2074 2499 3229 3325 3429 5036 6766 2074
Size: 425 730 96 104 1607 1730 2455
Fragments arranged by size:
2455 1730 1607 730 425 104 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3611 4502 844
Size: 2767 891 3489
Fragments arranged by size:
3489 2767 891
BsrBI CCG'CTC
Cuts at: 1394 1823 5034 1394
Size: 429 3211 3507
Fragments arranged by size:
3507 3211 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3253 3508 5642 5816 65
Size: 1183 2005 255 2134 174 1396
Fragments arranged by size:
2134 2005 1396 1183 255 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5791 5978 1700
Size: 110 3981 187 2869
Fragments arranged by size:
3981 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3633 96
Size: 3537 3610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7147
BssSI C'ACGA_G
Cuts at: 5207 6219 5207
Size: 1012 6135
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4409 794
Size: 3615 3532
BstBI TT'CG_AA
Cuts at: 5951 5951
Size: 7147
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7147
BstXI CCAn_nnnn'nTGG
Cuts at: 2282 2282
Size: 7147
BstYI r'GATC_y
Cuts at: 1972 2017 3060 3127 3566 3578 4477 4844 5229
Size: 45 1043 67 439 12 899 367 385
Cuts at: 5229 5246 6698 6784 6796 7142 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1043 899 439 385 367 346
86 67 45 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 3117 513
Size: 552 2052 4543
Fragments arranged by size:
4543 2052 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6408
Fragments arranged by size:
6408 683 56
BtsI GCAGTG_nn'
Cuts at: 2922 4245 4663 5457 5477 2922
Size: 1323 418 794 20 4592
Fragments arranged by size:
4592 1323 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 3235 1554
Size: 1681 5466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7112
DraI TTT'AAA
Cuts at: 2202 2863 4451 2202
Size: 661 1588 4898
Fragments arranged by size:
4898 1588 661
DraIII CAC_nnn'GTG
Cuts at: 4409 4409
Size: 7147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6154 809
Size: 507 4838 1802
Fragments arranged by size:
4838 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4594 5483 7112
Size: 54 1425 50 275 18 2764 889 1629
Cuts at: 7112 8
Size: 43
Fragments arranged by size:
2764 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4594 1537
Size: 275 2782 4090
Fragments arranged by size:
4090 2782 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 5077 1353
Size: 739 2985 3423
Fragments arranged by size:
3423 2985 739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5734 6108 6254 387
Size: 1064 138 4145 374 146 1280
Fragments arranged by size:
4145 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2438 3378 3510 3732 4258 5210
Size: 393 441 365 940 132 222 526 952
Cuts at: 5210 5807 6594 6862 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 952 940 787 597 526 441 393
365 268 222 132
EcoRI G'AATT_C
Cuts at: 3433 4456 4834 3433
Size: 1023 378 5746
Fragments arranged by size:
5746 1023 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2828 4439 2287
Size: 541 1611 4995
Fragments arranged by size:
4995 1611 541
FspI TGC'GCA
Cuts at: 5653 5653
Size: 7147
HaeII r_GCGC'y
Cuts at: 1678 6294 1678
Size: 4616 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2626 2658 3351
Size: 32 135 32 497 32 525 32 693
Cuts at: 3351 3383 3811 3843 4325 4357 5788 5820 5862
Size: 32 428 32 482 32 1431 32 42
Cuts at: 5862 5894 1373
Size: 32 2626
Fragments arranged by size:
2626 1431 693 525 497 482 428 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3317 4491 4737 669
Size: 988 281 1379 1174 246 3079
Fragments arranged by size:
3079 1379 1174 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2028 3917 748
Size: 1280 1889 3978
Fragments arranged by size:
3978 1889 1280
HpaI GTT'AAC
Cuts at: 4737 4737
Size: 7147
KpnI G_GTAC'C
Cuts at: 4472 4472
Size: 7147
MfeI C'AATT_G
Cuts at: 4019 4746 4019
Size: 727 6420
MluI A'CGCG_T
Cuts at: 7023 7023
Size: 7147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2045 6261 6445 1305
Size: 740 4216 184 2007
Fragments arranged by size:
4216 2007 740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5325
Fragments arranged by size:
5325 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3089 4365 5105 5464 5623 518
Size: 648 843 1080 1276 740 359 159 2042
Fragments arranged by size:
2042 1276 1080 843 740 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2716 4043 4563 5240 6388 6633 1606
Size: 1110 1327 520 677 1148 245 2120
Fragments arranged by size:
2120 1327 1148 1110 677 520 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6595
NdeI CA'TA_TG
Cuts at: 387 3022 387
Size: 2635 4512
NheI G'CTAG_C
Cuts at: 1052 2948 1052
Size: 1896 5251
NotI GC'GGCC_GC
Cuts at: 4594 4594
Size: 7147
NspI r_CATG'y
Cuts at: 1132 1229 2126 2465 4112 4505 6050 1132
Size: 97 897 339 1647 393 1545 2229
Fragments arranged by size:
2229 1647 1545 897 393 339 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7147
PciI A'CATG_T
Cuts at: 2122 4108 6046 2122
Size: 1986 1938 3223
Fragments arranged by size:
3223 1986 1938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7147
PfoI T'CCnGG_A
Cuts at: 4274 4274
Size: 7147
PmeI GTTT'AAAC
Cuts at: 4451 4451
Size: 7147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4170 4202 5216 5248 6729 6761 4170
Size: 32 1014 32 1481 32 4556
Fragments arranged by size:
4556 1481 1014 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7147
PsiI TTA'TAA
Cuts at: 2354 4717 2354
Size: 2363 4784
PstI C_TGCA'G
Cuts at: 830 4499 830
Size: 3669 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7147
PvuII CAG'CTG
Cuts at: 2716 4043 2716
Size: 1327 5820
SalI G'TCGA_C
Cuts at: 1936 4489 1936
Size: 2553 4594
SbfI CC_TGCA'GG
Cuts at: 4499 4499
Size: 7147
ScaI AGT'ACT
Cuts at: 2235 5395 7107 2235
Size: 3160 1712 2275
Fragments arranged by size:
3160 2275 1712
SfcI C'TryA_G
Cuts at: 826 1045 2103 3255 4495 5630 6311 6502 826
Size: 219 1058 1152 1240 1135 681 191 1471
Fragments arranged by size:
1471 1240 1152 1135 1058 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7147
SmaI CCC'GGG
Cuts at: 1872 4474 1872
Size: 2602 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 2687 4084 5251 6152 6414
Size: 197 926 318 426 1397 1167 901 262
Cuts at: 6414 820
Size: 1553
Fragments arranged by size:
1553 1397 1167 926 901 426 318 262
197
SnaBI TAC'GTA
Cuts at: 493 4938 493
Size: 4445 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7147
SphI G_CATG'C
Cuts at: 1132 4505 1132
Size: 3373 3774
SspI AAT'ATT
Cuts at: 5 52 3265 3751 5071 5
Size: 47 3213 486 1320 2081
Fragments arranged by size:
3213 2081 1320 486 47
StuI AGG'CCT
Cuts at: 1517 2062 2746 1517
Size: 545 684 5918
Fragments arranged by size:
5918 684 545
StyI C'CwwG_G
Cuts at: 513 1065 3639 4586 513
Size: 552 2574 947 3074
Fragments arranged by size:
3074 2574 947 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3345 5174 5191 5344 5529 5974 325
Size: 1255 1765 1829 17 153 185 445 1498
Fragments arranged by size:
1829 1765 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2233 2678 3633
Size: 274 80 33 51 1443 256 445 955
Cuts at: 3633 3935 5393 7105 96
Size: 302 1458 1712 138
Fragments arranged by size:
1712 1458 1443 955 445 302 274 256
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3570 3602 7107 7139 3570
Size: 32 3505 32 3578
Fragments arranged by size:
3578 3505 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7147
XbaI T'CTAG_A
Cuts at: 2492 4483 2492
Size: 1991 5156
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7147
XmaI C'CCGG_G
Cuts at: 1870 4472 1870
Size: 2602 4545
XmnI GAAnn'nnTTC
Cuts at: 3264 5276 3264
Size: 2012 5135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4955 276
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI