Restriction map for
pFN21AE5716
(Circular) (Six-base) MAPSORT of: pFN21AE5716.seq Check: 1931 from: 1 to: 6526
pFN21AE5716 6526 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3387 3641 3387
Size: 254 6272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2401 4336 278
Size: 53 83 186 936 865 1935 2468
Fragments arranged by size:
2468 1935 936 865 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3869 1937
Size: 1932 4594
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4589 5973 6241 1632
Size: 2957 1384 268 1917
Fragments arranged by size:
2957 1917 1384 268
AfeI AGC'GCT
Cuts at: 3068 3068
Size: 6526
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 5425 6402 1203
Size: 4222 977 1327
Fragments arranged by size:
4222 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 2164 5255 2164
Size: 3091 3435
AleI CACnn'nnGTG
Cuts at: 1166 3332 1166
Size: 2166 4360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 2442
Size: 32 6494
AlwNI CAG_nnn'CTG
Cuts at: 2215 2875 3286 3370 3395 3693 4269 5841 2215
Size: 660 411 84 25 298 576 1572 2900
Fragments arranged by size:
2900 1572 660 576 411 298 84 25
ApaI G_GGCC'C
Cuts at: 2857 2857
Size: 6526
ApaLI G'TGCA_C
Cuts at: 4583 5739 4583
Size: 1156 5370
ApoI r'AATT_y
Cuts at: 1493 2611 3354 3835 4061 4213 6322 1493
Size: 1118 743 481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 743 481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 2778 2850 2940 3178 3851 1870
Size: 73 361 474 72 90 238 673 4545
Fragments arranged by size:
4545 673 474 361 238 90 73 72
AvrII C'CTAG_G
Cuts at: 3269 3269
Size: 6526
BamHI G'GATC_C
Cuts at: 2800 3856 4223 2800
Size: 1056 367 5103
Fragments arranged by size:
5103 1056 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3587 3847 5302 618
Size: 325 217 627 1800 260 1455 1842
Fragments arranged by size:
1842 1800 1455 627 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2857 3324 3845 1391
Size: 1466 467 521 4072
Fragments arranged by size:
4072 1466 521 467
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5555
BbvCI CC'TCA_GC
Cuts at: 3396 3406 3545 3396
Size: 10 139 6377
Fragments arranged by size:
6377 139 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4717 4751 1903
Size: 34 50 34 2696 34 3678
Fragments arranged by size:
3678 2696 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 3132 1548
Size: 1584 4942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3482 3786 5137
Size: 107 122 71 631 605 1810 304 1351
Cuts at: 5137 136
Size: 1525
Fragments arranged by size:
1810 1525 1351 631 605 304 122 107
71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 3683 3943 3683
Size: 260 6266
Bme1580I G_kGCm'C
Cuts at: 1790 2857 4587 5743 1790
Size: 1067 1730 1156 2573
Fragments arranged by size:
2573 1730 1156 1067
BmgBI CAC'GTC
Cuts at: 1318 2755 1318
Size: 1437 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4257 5215 442
Size: 416 53 83 399 24 2840 958 1753
Fragments arranged by size:
2840 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2549 2664 3154 3753 5186 1239
Size: 844 466 115 490 599 1433 2579
Fragments arranged by size:
2579 1433 844 599 490 466 115
Bpu10I CC'TnA_GC
Cuts at: 3396 3406 3545 3396
Size: 10 139 6377
Fragments arranged by size:
6377 139 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2935 4651 5516 5814 2935
Size: 1716 865 298 3647
Fragments arranged by size:
3647 1716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3602 3746 5189 882
Size: 617 2103 144 1443 2219
Fragments arranged by size:
2219 2103 1443 617 144
BsaAI yAC'GTr
Cuts at: 493 1204 4317 493
Size: 711 3113 2702
Fragments arranged by size:
3113 2702 711
BsaBI GATnn'nnATC
Cuts at: 6520 6520
Size: 6526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2398 4333 4715 5350
Size: 53 83 186 936 865 1935 382 635
Cuts at: 5350 275
Size: 1451
Fragments arranged by size:
1935 1451 936 865 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4959 5357 5631 5778 6189 4959
Size: 398 274 147 411 5296
Fragments arranged by size:
5296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3332 3362 3751 3781 1178
Size: 30 165 30 1929 30 389 30 3923
Fragments arranged by size:
3923 1929 389 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2302 2485 2996 2999 3008 3674 3677
Size: 1105 11 183 511 3 9 666 3
Cuts at: 3677 3680 3713 3716 3725 3746 3764 1186
Size: 3 33 3 9 21 18 3948
Fragments arranged by size:
3948 1105 666 511 183 33 21 18
11 9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2215 2745 2951 3004 3808 5729 6225 2215
Size: 530 206 53 804 1921 496 2516
Fragments arranged by size:
2516 1921 804 530 496 206 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3383 1163
Size: 573 1647 4306
Fragments arranged by size:
4306 1647 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 3976 4737 5765 1540
Size: 275 186 1540 435 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 435 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 4587 4672 5743 2523
Size: 2064 85 1071 3306
Fragments arranged by size:
3306 2064 1071 85
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 2857 3324 3845 4587 4672 5743
Size: 399 733 334 467 521 742 85 1071
Cuts at: 5743 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 733 521 467 399 334
85
BspEI T'CCGG_A
Cuts at: 6189 6189
Size: 6526
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3271 3387 3641 3881 844
Size: 2427 116 254 240 3489
Fragments arranged by size:
3489 2427 254 240 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 2131 4413 1394
Size: 429 245 63 2282 3507
Fragments arranged by size:
3507 2282 429 245 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 3045 5021 5195 65
Size: 1183 1797 1976 174 1396
Fragments arranged by size:
1976 1797 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2323 5170 5357 1700
Size: 110 513 2847 187 2869
Fragments arranged by size:
2869 2847 513 187 110
BsrGI T'GTAC_A
Cuts at: 96 3498 96
Size: 3402 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 2223 4586 5598 2223
Size: 2363 1012 3151
Fragments arranged by size:
3151 2363 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3370 794
Size: 2576 3950
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 3085 1184
Size: 1901 4625
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 3619 2899
Size: 720 5806
BstYI r'GATC_y
Cuts at: 1972 2686 2800 3294 3312 3856 4223 4608 4625
Size: 714 114 494 18 544 367 385 17
Cuts at: 4625 6077 6163 6175 6521 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 714 544 494 385 367 346
114 86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3612 513
Size: 552 2547 3427
Fragments arranged by size:
3427 2547 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5787
Fragments arranged by size:
5787 683 56
BtsI GCAGTG_nn'
Cuts at: 3327 3537 4042 4836 4856 3327
Size: 210 505 794 20 4997
Fragments arranged by size:
4997 794 505 210 20
ClaI AT'CG_AT
Cuts at: 1554 2242 1554
Size: 688 5838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3538 3787 3973
Size: 54 1425 50 275 18 1708 249 186
Cuts at: 3973 4862 6491 8
Size: 889 1629 43
Fragments arranged by size:
1708 1629 1425 889 275 249 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 3973 1537
Size: 275 1726 435 4090
Fragments arranged by size:
4090 1726 435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2816 2985 3267 3666 3672 4456 1353
Size: 713 750 169 282 399 6 784 3423
Fragments arranged by size:
3423 784 750 713 399 282 169 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3166 5113 5487 5633 387
Size: 1064 138 1577 1947 374 146 1280
Fragments arranged by size:
1947 1577 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2549 2664 3154 3753 4589 5186
Size: 393 451 466 115 490 599 836 597
Cuts at: 5186 5973 6241 1239
Size: 787 268 1524
Fragments arranged by size:
1524 836 787 599 597 490 466 451
393 268 115
EcoO109I rG'GnC_Cy
Cuts at: 2782 2854 3367 3400 2782
Size: 72 513 33 5908
Fragments arranged by size:
5908 513 72 33
EcoRI G'AATT_C
Cuts at: 3354 3835 4213 3354
Size: 481 378 5667
Fragments arranged by size:
5667 481 378
EcoRV GAT'ATC
Cuts at: 2593 2593
Size: 6526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2486 2486
Size: 6526
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 3070 5673 1678
Size: 1392 2603 2531
Fragments arranged by size:
2603 2531 1392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 5167 5199 5241
Size: 32 135 32 578 32 2985 32 42
Cuts at: 5241 5273 1373
Size: 32 2626
Fragments arranged by size:
2985 2626 578 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3870 4116 669
Size: 988 281 1932 246 3079
Fragments arranged by size:
3079 1932 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2322 2620 2807 5640 5824 1305
Size: 825 192 298 187 2833 184 2007
Fragments arranged by size:
2833 2007 825 298 192 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4704
Fragments arranged by size:
4704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3332 4484 4843 5002 518
Size: 648 1731 435 1152 359 159 2042
Fragments arranged by size:
2042 1731 1152 648 435 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2545 2922 3410 3942 4619 5767 6012
Size: 512 427 377 488 532 677 1148 245
Cuts at: 6012 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 532 512 488 427 377
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5974
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 3884 5429 1132
Size: 97 2655 1545 2229
Fragments arranged by size:
2655 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2857 1348
Size: 1509 5017
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6526
PfoI T'CCnGG_A
Cuts at: 2355 3315 2355
Size: 960 5566
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2782 3400 2782
Size: 618 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 2853 2853
Size: 6526
PstI C_TGCA'G
Cuts at: 830 3878 830
Size: 3048 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2545 2922 2545
Size: 377 6149
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SanDI GG'GwC_CC
Cuts at: 2782 2782
Size: 6526
SapI GCTCTTCn'nnn_
Cuts at: 3672 3672
Size: 6526
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2318 3571 3874 5009 5690 5881 826
Size: 219 1273 1253 303 1135 681 191 1471
Fragments arranged by size:
1471 1273 1253 1135 681 303 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 2306 3180 3853 1872
Size: 434 874 673 4545
Fragments arranged by size:
4545 874 673 434
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2914 4630 5531 5793 820
Size: 197 926 971 1716 901 262 1553
Fragments arranged by size:
1716 1553 971 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 3246 1517
Size: 1729 4797
StyI C'CwwG_G
Cuts at: 513 1065 2405 2424 3269 3477 3965 513
Size: 552 1340 19 845 208 488 3074
Fragments arranged by size:
3074 1340 845 552 488 208 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 3402 3800 4553 4570 4723 4908
Size: 1255 582 1240 398 753 17 153 185
Cuts at: 4908 5353 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1240 753 582 445 398 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3498 4772 6484
Size: 274 80 33 51 1443 1521 1274 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
1712 1521 1443 1274 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 6486 6518 2758
Size: 32 3696 32 2766
Fragments arranged by size:
3696 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2573 1316
Size: 1257 5269
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 3097 3619 2468
Size: 629 522 5375
Fragments arranged by size:
5375 629 522
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 2304 3178 3851 1870
Size: 434 874 673 4545
Fragments arranged by size:
4545 874 673 434
XmnI GAAnn'nnTTC
Cuts at: 2021 4655 2021
Size: 2634 3892
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2399 4334 276
Size: 53 83 186 936 865 1935 2468
Fragments arranged by size:
2468 1935 936 865 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI