Restriction map for
pFN21AE5714
(Circular) (Six-base) MAPSORT of: pFN21AE5714.seq Check: 1825 from: 1 to: 7063
pFN21AE5714 7063 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2244 4873 278
Size: 53 83 186 936 708 2629 2468
Fragments arranged by size:
2629 2468 936 708 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3615 4406 1937
Size: 1678 791 4594
Fragments arranged by size:
4594 1678 791
Acc65I G'GTAC_C
Cuts at: 2577 4384 2577
Size: 1807 5256
AclI AA'CG_TT
Cuts at: 1563 5190 5563 1563
Size: 3627 373 3063
Fragments arranged by size:
3627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2967 5126 6510 6778 1632
Size: 1335 2159 1384 268 1917
Fragments arranged by size:
2159 1917 1384 1335 268
AflII C'TTAA_G
Cuts at: 820 1017 2557 820
Size: 197 1540 5326
Fragments arranged by size:
5326 1540 197
AflIII A'CryG_T
Cuts at: 1203 2184 2815 5962 6939 1203
Size: 981 631 3147 977 1327
Fragments arranged by size:
3147 1327 981 977 631
AgeI A'CCGG_T
Cuts at: 5894 5894
Size: 7063
AhdI GACnn_n'nnGTC
Cuts at: 5792 5792
Size: 7063
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2092 1184
Size: 908 6155
AlwNI CAG_nnn'CTG
Cuts at: 2329 4310 4806 6378 2329
Size: 1981 496 1572 3014
Fragments arranged by size:
3014 1981 1572 496
ApaI G_GGCC'C
Cuts at: 2106 2225 2106
Size: 119 6944
ApaLI G'TGCA_C
Cuts at: 4013 5120 6276 4013
Size: 1107 1156 4800
Fragments arranged by size:
4800 1156 1107
ApoI r'AATT_y
Cuts at: 1493 2300 2319 2880 3162 3492 3668 4063 4372
Size: 807 19 561 282 330 176 395 309
Cuts at: 4372 4598 4750 6859 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 807 561 395 330 309 282
226 176 152 19
AseI AT'TA_AT
Cuts at: 160 1032 5617 160
Size: 872 4585 1606
Fragments arranged by size:
4585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 4388 1870
Size: 73 168 2277 4545
Fragments arranged by size:
4545 2277 168 73
BamHI G'GATC_C
Cuts at: 3511 4393 4760 3511
Size: 882 367 5814
Fragments arranged by size:
5814 882 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2049 2577 4139 4384 5839
Size: 325 217 627 262 528 1562 245 1455
Cuts at: 5839 618
Size: 1842
Fragments arranged by size:
1842 1562 1455 627 528 325 262 245
217
BanII G_rGCy'C
Cuts at: 1391 2035 2106 2225 3810 4382 1391
Size: 644 71 119 1585 572 4072
Fragments arranged by size:
4072 1585 644 572 119 71
BbeI G_GCGC'C
Cuts at: 2053 2053
Size: 7063
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3524 928
Size: 971 1625 4467
Fragments arranged by size:
4467 1625 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5254 5288 1903
Size: 34 3317 34 3678
Fragments arranged by size:
3678 3317 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4955 6165 4955
Size: 1210 5853
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7063
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 5674 136
Size: 107 122 71 631 605 386 3616 1525
Fragments arranged by size:
3616 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 2721 3472 3973 7058 2721
Size: 751 501 3085 2726
Fragments arranged by size:
3085 2726 751 501
BlpI GC'TnA_GC
Cuts at: 3546 3926 4480 3546
Size: 380 554 6129
Fragments arranged by size:
6129 554 380
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2106 2202 2225 4017 5124 6280 1790
Size: 233 83 96 23 1792 1107 1156 2573
Fragments arranged by size:
2573 1792 1156 1107 233 96 83 23
BmgBI CAC'GTC
Cuts at: 1318 2187 1318
Size: 869 6194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2339 3336 4794
Size: 416 53 83 399 24 922 997 1458
Cuts at: 4794 5752 442
Size: 958 1753
Fragments arranged by size:
1753 1458 997 958 922 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 3962 4221 5723 1239
Size: 1464 1259 259 1502 2579
Fragments arranged by size:
2579 1502 1464 1259 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2512 3207 3375 5188 6053 6351 2512
Size: 695 168 1813 865 298 3224
Fragments arranged by size:
3224 1813 865 695 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5726 882
Size: 617 4227 2219
Fragments arranged by size:
4227 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2543 4854 493
Size: 711 1339 2311 2702
Fragments arranged by size:
2702 2311 1339 711
BsaBI GATnn'nnATC
Cuts at: 3175 7057 3175
Size: 3882 3181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2050 2241 4870 5252
Size: 53 83 186 936 517 191 2629 382
Cuts at: 5252 5887 275
Size: 635 1451
Fragments arranged by size:
2629 1451 936 635 517 382 191 186
83 53
BsaWI w'CCGG_w
Cuts at: 2162 5496 5894 6168 6315 6726 2162
Size: 3334 398 274 147 411 2499
Fragments arranged by size:
3334 2499 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2987 3017 1178
Size: 30 165 30 1584 30 5224
Fragments arranged by size:
5224 1584 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2143 4220 1186
Size: 954 3 2077 4029
Fragments arranged by size:
4029 2077 954 3
BseYI C'CCAG_C
Cuts at: 2225 4175 6266 6762 2225
Size: 1950 2091 496 2526
Fragments arranged by size:
2526 2091 1950 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 2860 3712 4238 1163
Size: 573 742 382 852 526 3988
Fragments arranged by size:
3988 852 742 573 526 382
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4513 5274 6302 1540
Size: 275 186 2512 761 1028 2301
Fragments arranged by size:
2512 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2929 4017 5124 5209 6280 2929
Size: 1088 1107 85 1071 3712
Fragments arranged by size:
3712 1107 1088 1071 85
BsmI GAATG_Cn'
Cuts at: 4574 4667 4574
Size: 93 6970
BsmBI CGTCTCn'nnnn_
Cuts at: 2363 4040 2363
Size: 1677 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2035 2106 2202 2225 2929 3810
Size: 399 233 12 71 96 23 704 881
Cuts at: 3810 4017 4382 5124 5209 6280 1391
Size: 207 365 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 704 399 365 233
207 96 85 71 23 12
BspEI T'CCGG_A
Cuts at: 6726 6726
Size: 7063
BspHI T'CATG_A
Cuts at: 3466 4952 6682 3466
Size: 1486 1730 3847
Fragments arranged by size:
3847 1730 1486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2980 4418 844
Size: 2136 1438 3489
Fragments arranged by size:
3489 2136 1438
BsrBI CCG'CTC
Cuts at: 1394 1823 4950 1394
Size: 429 3127 3507
Fragments arranged by size:
3507 3127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5558 5732 65
Size: 1183 4310 174 1396
Fragments arranged by size:
4310 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5707 5894 1700
Size: 110 3897 187 2869
Fragments arranged by size:
3897 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2825 3003 96
Size: 2729 178 4156
Fragments arranged by size:
4156 2729 178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7063
BssSI C'ACGA_G
Cuts at: 5123 6135 5123
Size: 1012 6051
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 5392
BstBI TT'CG_AA
Cuts at: 2727 5867 2727
Size: 3140 3923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7063
BstXI CCAn_nnnn'nTGG
Cuts at: 2574 2574
Size: 7063
BstYI r'GATC_y
Cuts at: 1972 2721 2896 3472 3511 3883 3910 3973 4393
Size: 749 175 576 39 372 27 63 420
Cuts at: 4393 4760 5145 5162 6614 6700 6712 7058 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 749 576 420 385 372 367
346 175 86 63 39 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2217 2217
Size: 7063
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6324
Fragments arranged by size:
6324 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 4579 5373 5393 2848
Size: 1731 794 20 4518
Fragments arranged by size:
4518 1731 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7063
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7028
DraI TTT'AAA
Cuts at: 3439 3667 4367 3439
Size: 228 700 6135
Fragments arranged by size:
6135 700 228
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 3113 6070 809
Size: 507 924 873 2957 1802
Fragments arranged by size:
2957 1802 924 873 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 4510 5399
Size: 54 1425 50 275 18 229 2451 889
Cuts at: 5399 7028 8
Size: 1629 43
Fragments arranged by size:
2451 1629 1425 889 275 229 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4510 1537
Size: 275 2698 4090
Fragments arranged by size:
4090 2698 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 2769 2943 3494 4993 1353
Size: 1013 403 174 551 1499 3423
Fragments arranged by size:
3423 1499 1013 551 403 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5650 6024 6170 387
Size: 1064 138 4061 374 146 1280
Fragments arranged by size:
4061 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2703 2967 3962 4221 5126 5723 6510
Size: 393 1071 264 995 259 905 597 787
Cuts at: 6510 6778 1239
Size: 268 1524
Fragments arranged by size:
1524 1071 995 905 787 597 393 268
264 259
EcoO109I rG'GnC_Cy
Cuts at: 2103 2221 2222 2624 2103
Size: 118 1 402 6542
Fragments arranged by size:
6542 402 118 1
EcoRI G'AATT_C
Cuts at: 4372 4750 4372
Size: 378 6685
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 7063
FspI TGC'GCA
Cuts at: 5569 5569
Size: 7063
HaeII r_GCGC'y
Cuts at: 1678 2053 2232 6210 1678
Size: 375 179 3978 2531
Fragments arranged by size:
3978 2531 375 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2881 2913 5704 5736 5778
Size: 32 135 32 1309 32 2791 32 42
Cuts at: 5778 5810 1373
Size: 32 2626
Fragments arranged by size:
2791 2626 1309 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4407 4653 669
Size: 988 281 2469 246 3079
Fragments arranged by size:
3079 2469 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2370 2380 748
Size: 1622 10 5431
Fragments arranged by size:
5431 1622 10
HpaI GTT'AAC
Cuts at: 4653 4653
Size: 7063
KasI G'GCGC_C
Cuts at: 2049 2049
Size: 7063
KpnI G_GTAC'C
Cuts at: 2581 4388 2581
Size: 1807 5256
MfeI C'AATT_G
Cuts at: 3282 4662 3282
Size: 1380 5683
MluI A'CGCG_T
Cuts at: 6939 6939
Size: 7063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 3563 6177 6361 1305
Size: 1197 1061 2614 184 2007
Fragments arranged by size:
2614 2007 1197 1061 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2061 10
Size: 54 1425 343 229 5012
Fragments arranged by size:
5012 1425 343 229 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 2961 3059 5021 5380 5539 518
Size: 648 1303 492 98 1962 359 159 2042
Fragments arranged by size:
2042 1962 1303 648 492 359 159 98
MspA1I CmG'CkG
Cuts at: 1606 2602 3182 3527 4479 5156 6304 6549 1606
Size: 996 580 345 952 677 1148 245 2120
Fragments arranged by size:
2120 1148 996 952 677 580 345 245
NarI GG'CG_CC
Cuts at: 2050 2050
Size: 7063
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6511
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7063
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7063
NotI GC'GGCC_GC
Cuts at: 4510 4510
Size: 7063
NspI r_CATG'y
Cuts at: 1132 1229 2501 2819 4246 4421 5966 1132
Size: 97 1272 318 1427 175 1545 2229
Fragments arranged by size:
2229 1545 1427 1272 318 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7063
PciI A'CATG_T
Cuts at: 2815 5962 2815
Size: 3147 3916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2910 1108
Size: 1802 5261
PmeI GTTT'AAAC
Cuts at: 4367 4367
Size: 7063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5132 5164 6645 6677 5132
Size: 32 1481 32 5518
Fragments arranged by size:
5518 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7063
PsiI TTA'TAA
Cuts at: 3335 4633 3335
Size: 1298 5765
PspOMI G'GGCC_C
Cuts at: 2102 2221 2102
Size: 119 6944
PstI C_TGCA'G
Cuts at: 830 3402 3679 4415 830
Size: 2572 277 736 3478
Fragments arranged by size:
3478 2572 736 277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7063
PvuII CAG'CTG
Cuts at: 3182 3527 3182
Size: 345 6718
SalI G'TCGA_C
Cuts at: 1936 4405 1936
Size: 2469 4594
SapI GCTCTTCn'nnn_
Cuts at: 2769 2769
Size: 7063
SbfI CC_TGCA'GG
Cuts at: 4415 4415
Size: 7063
ScaI AGT'ACT
Cuts at: 5311 7023 5311
Size: 1712 5351
SexAI A'CCwGG_T
Cuts at: 2985 2985
Size: 7063
SfcI C'TryA_G
Cuts at: 826 1045 3273 3398 3675 4411 5546 6227 6418
Size: 219 2228 125 277 736 1135 681 191
Cuts at: 6418 826
Size: 1471
Fragments arranged by size:
2228 1471 1135 736 681 277 219 191
125
SfoI GGC'GCC
Cuts at: 2051 2051
Size: 7063
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7063
SmaI CCC'GGG
Cuts at: 1872 2113 4390 1872
Size: 241 2277 4545
Fragments arranged by size:
4545 2277 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2491 2557 3222 3390 5167 6068
Size: 197 926 548 66 665 168 1777 901
Cuts at: 6068 6330 820
Size: 262 1553
Fragments arranged by size:
1777 1553 926 901 665 548 262 197
168 66
SnaBI TAC'GTA
Cuts at: 493 2543 4854 493
Size: 2050 2311 2702
Fragments arranged by size:
2702 2311 2050
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7063
SphI G_CATG'C
Cuts at: 1132 4421 1132
Size: 3289 3774
SspI AAT'ATT
Cuts at: 5 52 2511 2613 3868 4987 5
Size: 47 2459 102 1255 1119 2081
Fragments arranged by size:
2459 2081 1255 1119 102 47
StuI AGG'CCT
Cuts at: 1517 3505 1517
Size: 1988 5075
StyI C'CwwG_G
Cuts at: 513 1065 4502 513
Size: 552 3437 3074
Fragments arranged by size:
3437 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 4189 5090 5107 5260 5445 5890
Size: 1255 741 1868 901 17 153 185 445
Cuts at: 5890 325
Size: 1498
Fragments arranged by size:
1868 1498 1255 901 741 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2825 3003 5309
Size: 274 80 33 51 1443 848 178 2306
Cuts at: 5309 7021 96
Size: 1712 138
Fragments arranged by size:
2306 1712 1443 848 274 178 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7023 7055 7023
Size: 32 7031
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7063
XbaI T'CTAG_A
Cuts at: 4399 4399
Size: 7063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2907 3061 2907
Size: 154 6909
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7063
XmaI C'CCGG_G
Cuts at: 1870 2111 4388 1870
Size: 241 2277 4545
Fragments arranged by size:
4545 2277 241
XmnI GAAnn'nnTTC
Cuts at: 2666 2952 5192 2666
Size: 286 2240 4537
Fragments arranged by size:
4537 2240 286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2242 4871 276
Size: 53 83 186 936 708 2629 2468
Fragments arranged by size:
2629 2468 936 708 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SrfI SwaI