Restriction map for
pFN21AE5705
(Circular) (Six-base) MAPSORT of: pFN21AE5705.seq Check: 566 from: 1 to: 6574
pFN21AE5705 6574 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2763 2763
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2315 2361 4637 6021 6289 1632
Size: 375 308 46 2276 1384 268 1917
Fragments arranged by size:
2276 1917 1384 375 308 268 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 2475 5303 2475
Size: 2828 3746
AleI CACnn'nnGTG
Cuts at: 1166 3281 1166
Size: 2115 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 2271 3092 3203 4317 5889 2271
Size: 821 111 1114 1572 2956
Fragments arranged by size:
2956 1572 1114 821 111
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 2971 3603 3883 4109 4261 6370 1493
Size: 1478 632 280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1478 632 280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3250 3575 3830 3899 1870
Size: 73 1307 325 255 69 4545
Fragments arranged by size:
4545 1307 325 255 73 69
BamHI G'GATC_C
Cuts at: 2345 2790 3904 4271 2345
Size: 445 1114 367 4648
Fragments arranged by size:
4648 1114 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3176 3272 3895 5350 618
Size: 325 217 627 1389 96 623 1455 1842
Fragments arranged by size:
1842 1455 1389 627 623 325 217 96
BanII G_rGCy'C
Cuts at: 1391 2829 3004 3543 3576 3893 1391
Size: 1438 175 539 33 317 4072
Fragments arranged by size:
4072 1438 539 317 175 33
BbeI G_GCGC'C
Cuts at: 3180 3180
Size: 6574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 3647 928
Size: 971 423 1325 3855
Fragments arranged by size:
3855 1325 971 423
BbvCI CC'TCA_GC
Cuts at: 3102 3102
Size: 6574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2162 2196 4765 4799 1903
Size: 34 50 34 141 34 2569 34 3678
Fragments arranged by size:
3678 2569 141 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 2370 1548
Size: 822 5752
BfrBI ATG'CAT
Cuts at: 3298 3298
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5185 136
Size: 107 122 71 631 605 3513 1525
Fragments arranged by size:
3513 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3081 6569 3081
Size: 3488 3086
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2189 4635 5791 1790
Size: 399 2446 1156 2573
Fragments arranged by size:
2573 2446 1156 399
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2519 2733 4305
Size: 416 53 83 399 24 1102 214 1572
Cuts at: 4305 5263 442
Size: 958 1753
Fragments arranged by size:
1753 1572 1102 958 416 399 214 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3018 3018
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 3068 5234 1239
Size: 989 840 2166 2579
Fragments arranged by size:
2579 2166 989 840
Bpu10I CC'TnA_GC
Cuts at: 2481 2819 3102 3140 2481
Size: 338 283 38 5915
Fragments arranged by size:
5915 338 283 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2813 3332 4699 5564 5862 2813
Size: 519 1367 865 298 3525
Fragments arranged by size:
3525 1367 865 519 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2897 5237 882
Size: 617 1398 2340 2219
Fragments arranged by size:
2340 2219 1398 617
BsaAI yAC'GTr
Cuts at: 493 1204 4365 493
Size: 711 3161 2702
Fragments arranged by size:
3161 2702 711
BsaBI GATnn'nnATC
Cuts at: 2115 6568 2115
Size: 4453 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2733 3177 4381 4763
Size: 53 83 186 936 1200 444 1204 382
Cuts at: 4763 5398 275
Size: 635 1451
Fragments arranged by size:
1451 1204 1200 936 635 444 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5007 5405 5679 5826 6237 5007
Size: 398 274 147 411 5344
Fragments arranged by size:
5344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2852 2882 1178
Size: 30 165 30 1449 30 4870
Fragments arranged by size:
4870 1449 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 3119 1186
Size: 1306 627 4641
Fragments arranged by size:
4641 1306 627
BseYI C'CCAG_C
Cuts at: 5777 6273 5777
Size: 496 6078
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3390 1163
Size: 573 1654 4347
Fragments arranged by size:
4347 1654 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2658 2754 2829 3259 4635 4720 5791 2658
Size: 96 75 430 1376 85 1071 3441
Fragments arranged by size:
3441 1376 1071 430 96 85 75
BsmI GAATG_Cn'
Cuts at: 3335 4085 4178 3335
Size: 750 93 5731
Fragments arranged by size:
5731 750 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2690 3200 3842 2690
Size: 510 642 5422
Fragments arranged by size:
5422 642 510
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2658 2754 2829 3004 3259 3543
Size: 399 399 469 96 75 175 255 284
Cuts at: 3543 3576 3893 4635 4720 5791 1391
Size: 33 317 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 399 317 284
255 175 96 85 75 33
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 3785 3929 844
Size: 1919 1022 144 3489
Fragments arranged by size:
3489 1919 1022 144
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5069 5243 65
Size: 1183 3821 174 1396
Fragments arranged by size:
3821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2420 5218 5405 1700
Size: 110 610 2798 187 2869
Fragments arranged by size:
2869 2798 610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6574
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 2720 3398 1184
Size: 1536 678 4360
Fragments arranged by size:
4360 1536 678
BstXI CCAn_nnnn'nTGG
Cuts at: 2260 2260
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 2345 2790 3081 3904 4271 4656 4673 6125
Size: 373 445 291 823 367 385 17 1452
Cuts at: 6125 6211 6223 6569 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 823 445 385 373 367 346
291 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2938 487
Size: 683 56 1712 4123
Fragments arranged by size:
4123 1712 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 4090 4884 4904 2601
Size: 1489 794 20 4271
Fragments arranged by size:
4271 1489 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2422 4021
Size: 54 1425 50 275 18 570 22 1599
Cuts at: 4021 4910 6539 8
Size: 889 1629 43
Fragments arranged by size:
1629 1599 1425 889 570 275 54 50
43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2714 4504 1353
Size: 846 515 1790 3423
Fragments arranged by size:
3423 1790 846 515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2827 2827
Size: 6574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2228 2315 2361 3068 4637 5234
Size: 393 375 221 87 46 707 1569 597
Cuts at: 5234 6021 6289 1239
Size: 787 268 1524
Fragments arranged by size:
1569 1524 787 707 597 393 375 268
221 87 46
EcoNI CCTnn'n_nnAGG
Cuts at: 3230 3230
Size: 6574
EcoO109I rG'GnC_Cy
Cuts at: 2833 3167 3216 3306 3503 2833
Size: 334 49 90 197 5904
Fragments arranged by size:
5904 334 197 90 49
EcoRI G'AATT_C
Cuts at: 2971 3603 3883 4261 2971
Size: 632 280 378 5284
Fragments arranged by size:
5284 632 378 280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3349 3419 3349
Size: 70 6504
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2236 2488 3180 5721 1678
Size: 558 252 692 2541 2531
Fragments arranged by size:
2541 2531 692 558 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2461 2493 5215 5247 5289
Size: 32 135 32 889 32 2722 32 42
Cuts at: 5289 5321 1373
Size: 32 2626
Fragments arranged by size:
2722 2626 889 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 3918 4164 669
Size: 988 281 520 1460 246 3079
Fragments arranged by size:
3079 1460 988 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 3176 3176
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5688 5872 1305
Size: 4383 184 2007
Fragments arranged by size:
4383 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 10
Size: 54 1425 343 570 4182
Fragments arranged by size:
4182 1425 570 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2121 3281 3362 4532 4891 5050 518
Size: 648 955 1160 81 1170 359 159 2042
Fragments arranged by size:
2042 1170 1160 955 648 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 2041 2925 2978 3089 3990 4667 5815 6060
Size: 435 884 53 111 901 677 1148 245
Cuts at: 6060 1606
Size: 2120
Fragments arranged by size:
2120 1148 901 884 677 435 245 111
53
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 6574
NarI GG'CG_CC
Cuts at: 3177 3177
Size: 6574
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6022
NdeI CA'TA_TG
Cuts at: 387 3340 387
Size: 2953 3621
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NsiI A_TGCA'T
Cuts at: 3300 3300
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2213 3875 3932 5477 1132
Size: 97 984 1662 57 1545 2229
Fragments arranged by size:
2229 1662 1545 984 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6574
PfoI T'CCnGG_A
Cuts at: 2534 2534
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3167 3216 3306 3503 3167
Size: 49 90 197 6238
Fragments arranged by size:
6238 197 90 49
PshAI GACnn'nnGTC
Cuts at: 1613 2769 1613
Size: 1156 5418
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PstI C_TGCA'G
Cuts at: 830 3782 3926 830
Size: 2952 144 3478
Fragments arranged by size:
3478 2952 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2041 2978 3089 2041
Size: 937 111 5526
Fragments arranged by size:
5526 937 111
SacI G_AGCT'C
Cuts at: 2829 2829
Size: 6574
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SanDI GG'GwC_CC
Cuts at: 3167 3306 3167
Size: 139 6435
SapI GCTCTTCn'nnn_
Cuts at: 2199 2714 2199
Size: 515 6059
SbfI CC_TGCA'GG
Cuts at: 3782 3926 3782
Size: 144 6430
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SfcI C'TryA_G
Cuts at: 826 1045 2379 3312 3778 3922 5057 5738 5929
Size: 219 1334 933 466 144 1135 681 191
Cuts at: 5929 826
Size: 1471
Fragments arranged by size:
1471 1334 1135 933 681 466 219 191
144
SfoI GGC'GCC
Cuts at: 3178 3178
Size: 6574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3577 3901 1872
Size: 1705 324 4545
Fragments arranged by size:
4545 1705 324
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 3347 3830 4678 5579 5841
Size: 197 926 885 519 483 848 901 262
Cuts at: 5841 820
Size: 1553
Fragments arranged by size:
1553 926 901 885 848 519 483 262
197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 2213 3932 1132
Size: 1081 1719 3774
Fragments arranged by size:
3774 1719 1081
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 2090 3444 1517
Size: 573 1354 4647
Fragments arranged by size:
4647 1354 573
StyI C'CwwG_G
Cuts at: 513 1065 2607 2855 2913 3543 4013 513
Size: 552 1542 248 58 630 470 3074
Fragments arranged by size:
3074 1542 630 552 470 248 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 4601 4618 4771 4956 5401 325
Size: 1255 510 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 510 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2634 2666 2852 2884 6534 6566 2634
Size: 32 186 32 3650 32 2642
Fragments arranged by size:
3650 2642 186 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3220 1316
Size: 1904 4670
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 3830 1943
Size: 1887 4687
XmaI C'CCGG_G
Cuts at: 1870 3575 3899 1870
Size: 1705 324 4545
Fragments arranged by size:
4545 1705 324
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI
SwaI