Restriction map for
pFN21AE5692
(Circular) (Six-base) MAPSORT of: pFN21AE5692.seq Check: 6954 from: 1 to: 6208
pFN21AE5692 6208 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2465 4018 278
Size: 53 83 186 936 929 1553 2468
Fragments arranged by size:
2468 1553 936 929 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3551 1937
Size: 718 896 4594
Fragments arranged by size:
4594 896 718
Acc65I G'GTAC_C
Cuts at: 2117 2803 3529 2117
Size: 686 726 4796
Fragments arranged by size:
4796 726 686
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2348 2765 2921 3122 4271 5655 5923
Size: 563 153 417 156 201 1149 1384 268
Cuts at: 5923 1632
Size: 1917
Fragments arranged by size:
1917 1384 1149 563 417 268 201 156
153
AfeI AGC'GCT
Cuts at: 3440 3440
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 2443 4937 2443
Size: 2494 3714
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3000 1184
Size: 1816 4392
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 6176
AlwNI CAG_nnn'CTG
Cuts at: 2493 2590 2763 2788 2950 3951 5523 2493
Size: 97 173 25 162 1001 1572 3178
Fragments arranged by size:
3178 1572 1001 173 162 97 25
ApaI G_GGCC'C
Cuts at: 2887 2887
Size: 6208
ApaLI G'TGCA_C
Cuts at: 3173 4265 5421 3173
Size: 1092 1156 3960
Fragments arranged by size:
3960 1156 1092
ApoI r'AATT_y
Cuts at: 1493 2168 3517 3743 3895 6004 1493
Size: 675 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 675 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 3533 1870
Size: 73 730 860 4545
Fragments arranged by size:
4545 860 730 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2101 2134 2101
Size: 33 6175
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 2803 2999 3452 3529
Size: 325 217 627 330 686 196 453 77
Cuts at: 3529 4984 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 686 627 453 330 325 217
196 77
BanII G_rGCy'C
Cuts at: 1391 2035 2103 2887 3204 3527 1391
Size: 644 68 784 317 323 4072
Fragments arranged by size:
4072 784 644 323 317 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3096 3202 928
Size: 971 1197 106 3934
Fragments arranged by size:
3934 1197 971 106
BbvCI CC'TCA_GC
Cuts at: 2809 3053 2809
Size: 244 5964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2048 2333 4100 5310 2048
Size: 285 1767 1210 2946
Fragments arranged by size:
2946 1767 1210 285
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4819 136
Size: 107 122 71 631 605 3147 1525
Fragments arranged by size:
3147 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2887 3004 3177 4269 5425 1790
Size: 337 760 117 173 1092 1156 2573
Fragments arranged by size:
2573 1156 1092 760 337 173 117
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3011 3939 4897
Size: 416 53 83 399 24 1594 928 958
Cuts at: 4897 442
Size: 1753
Fragments arranged by size:
1753 1594 958 928 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2494 2684 2987 4868 1239
Size: 1255 190 303 1881 2579
Fragments arranged by size:
2579 1881 1255 303 190
Bpu10I CC'TnA_GC
Cuts at: 2552 2809 3053 3198 2552
Size: 257 244 145 5562
Fragments arranged by size:
5562 257 244 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 4333 5198 5496 2258
Size: 2075 865 298 2970
Fragments arranged by size:
2970 2075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 2963 4871 882
Size: 617 921 543 1908 2219
Fragments arranged by size:
2219 1908 921 617 543
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2462 4015 4397 5032
Size: 53 83 186 936 929 1553 382 635
Cuts at: 5032 275
Size: 1451
Fragments arranged by size:
1553 1451 936 929 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2151 4641 5039 5313 5460 5871 2151
Size: 2490 398 274 147 411 2488
Fragments arranged by size:
2490 2488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 3054 3084 3113
Size: 30 165 30 837 30 784 30 29
Cuts at: 3113 3143 1178
Size: 30 4243
Fragments arranged by size:
4243 837 784 165 30 30 30 30
30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3053 3062 1186
Size: 1867 9 4332
Fragments arranged by size:
4332 1867 9
BseYI C'CCAG_C
Cuts at: 2026 2767 3441 3464 5411 5907 2026
Size: 741 674 23 1947 496 2327
Fragments arranged by size:
2327 1947 741 674 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 3045 1163
Size: 573 498 811 4326
Fragments arranged by size:
4326 811 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3658 4419 5447 1540
Size: 275 186 1222 435 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 435 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 3177 4269 4354 5425 2035
Size: 1142 1092 85 1071 2818
Fragments arranged by size:
2818 1142 1092 1071 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 3035 2711
Size: 324 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2103 2127 2887 3004 3177 3204
Size: 399 245 68 24 760 117 173 27
Cuts at: 3204 3527 4269 4354 5425 1391
Size: 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 760 742 399 323 245 173
117 85 68 27 24
BspEI T'CCGG_A
Cuts at: 2151 5871 2151
Size: 3720 2488
BspHI T'CATG_A
Cuts at: 2835 3085 4097 5827 2835
Size: 250 1012 1730 3216
Fragments arranged by size:
3216 1730 1012 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 3563 844
Size: 1754 965 3489
Fragments arranged by size:
3489 1754 965
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2046 4703 4877 65
Size: 1183 798 2657 174 1396
Fragments arranged by size:
2657 1396 1183 798 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2590 794
Size: 1796 4412
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2416 3279 3538 3905 4290 4307 5759 5845
Size: 444 863 259 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 863 444 385 367 346 259
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3074 3074
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 2142 3136 513
Size: 552 939 89 49 994 3585
Fragments arranged by size:
3585 994 939 552 89 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 3347 3475 3347
Size: 128 6080
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2703 2977 5215 809
Size: 507 1387 274 2238 1802
Fragments arranged by size:
2238 1802 1387 507 274
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 3655 4544
Size: 54 1425 50 275 18 304 1521 889
Cuts at: 4544 6173 8
Size: 1629 43
Fragments arranged by size:
1629 1521 1425 889 304 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4138 1353
Size: 2785 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3122 4795 5169 5315 387
Size: 1064 138 1533 1673 374 146 1280
Fragments arranged by size:
1673 1533 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2033 2033
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2348 2494 2684 2765 2921 2987
Size: 393 563 153 146 190 81 156 66
Cuts at: 2987 3122 4271 4868 5655 5923 1239
Size: 135 1149 597 787 268 1524
Fragments arranged by size:
1524 1149 787 597 563 393 268 190
156 153 146 135 81 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2594 2954 2594
Size: 360 5848
EcoO109I rG'GnC_Cy
Cuts at: 2587 2867 2884 2947 2587
Size: 280 17 63 5848
Fragments arranged by size:
5848 280 63 17
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2848 2998 2848
Size: 150 6058
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 3442 5355 1678
Size: 1764 1913 2531
Fragments arranged by size:
2531 1913 1764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2429 2461 4849
Size: 32 135 32 668 32 157 32 2388
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2388 668 157 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3430 3552 3798 669
Size: 988 281 1492 122 246 3079
Fragments arranged by size:
3079 1492 988 281 246 122
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 2121 2807 3533 2121
Size: 686 726 4796
Fragments arranged by size:
4796 726 686
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 5322 5506 1305
Size: 799 3218 184 2007
Fragments arranged by size:
3218 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2136 10
Size: 54 1425 343 304 4082
Fragments arranged by size:
4082 1425 343 304 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 4166 4525 4684 518
Size: 648 1033 1967 359 159 2042
Fragments arranged by size:
2042 1967 1033 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 3624 4301 5449 5694 1606
Size: 937 1081 677 1148 245 2120
Fragments arranged by size:
2120 1148 1081 937 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2093 2142 3136 513
Size: 552 939 89 49 994 3585
Fragments arranged by size:
3585 994 939 552 89 49
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2779 3566 5111 1132
Size: 97 1550 787 1545 2229
Fragments arranged by size:
2229 1550 1545 787 97
PasI CC'CwG_GG
Cuts at: 1348 2854 3300 3326 1348
Size: 1506 446 26 4230
Fragments arranged by size:
4230 1506 446 26
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 2472 2493 2950 3300 1108
Size: 1035 329 21 457 350 4016
Fragments arranged by size:
4016 1035 457 350 329 21
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4277 4309 5790 5822 3113
Size: 32 1132 32 1481 32 3499
Fragments arranged by size:
3499 1481 1132 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2947 2587
Size: 360 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 2883 2883
Size: 6208
PstI C_TGCA'G
Cuts at: 830 3560 830
Size: 2730 3478
PvuI CG_AT'CG
Cuts at: 2001 3223 2001
Size: 1222 4986
PvuII CAG'CTG
Cuts at: 2543 2543
Size: 6208
SacI G_AGCT'C
Cuts at: 2035 2035
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 2231 4456 6168 2231
Size: 2225 1712 2271
Fragments arranged by size:
2271 2225 1712
SfcI C'TryA_G
Cuts at: 826 1045 2594 3501 3556 4691 5372 5563 826
Size: 219 1549 907 55 1135 681 191 1471
Fragments arranged by size:
1549 1471 1135 907 681 219 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2675 3535 1872
Size: 803 860 4545
Fragments arranged by size:
4545 860 803
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 4312 5213 5475 820
Size: 197 926 330 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 330 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6208
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 2142 2645 3136 3647 513
Size: 552 939 89 49 503 491 511 3074
Fragments arranged by size:
3074 939 552 511 503 491 89 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2922 2942 3079 4235 4252 4405 4590
Size: 1255 1342 20 137 1156 17 153 185
Cuts at: 4590 5035 325
Size: 445 1498
Fragments arranged by size:
1498 1342 1255 1156 445 185 153 137
20 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 4454 6166
Size: 274 80 33 51 1443 252 2225 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
2225 1712 1443 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 6168 6200 3054
Size: 32 3082 32 3062
Fragments arranged by size:
3082 3062 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3022 3544 3022
Size: 522 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2673 3533 1870
Size: 803 860 4545
Fragments arranged by size:
4545 860 803
XmnI GAAnn'nnTTC
Cuts at: 2916 4337 2916
Size: 1421 4787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2463 4016 276
Size: 53 83 186 936 929 1553 2468
Fragments arranged by size:
2468 1553 936 929 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BfrBI BplI BsiWI BstXI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI