Restriction map for
pFN21AE5686
(Circular) (Six-base) MAPSORT of: pFN21AE5686.seq Check: 2020 from: 1 to: 6394
pFN21AE5686 6394 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3737 1937
Size: 1800 4594
Acc65I G'GTAC_C
Cuts at: 2511 3715 2511
Size: 1204 5190
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3167 3332 4457 5841 6109 1632
Size: 1535 165 1125 1384 268 1917
Fragments arranged by size:
1917 1535 1384 1125 268 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 2307 5293 6270 1203
Size: 1104 2986 977 1327
Fragments arranged by size:
2986 1327 1104 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 2946 1166
Size: 1780 4614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3593 1184
Size: 2409 3985
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 6362
AlwNI CAG_nnn'CTG
Cuts at: 2182 4137 5709 2182
Size: 1955 1572 2867
Fragments arranged by size:
2867 1955 1572
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 3703 3929 4081 6190 1493
Size: 2210 226 152 2109 1697
Fragments arranged by size:
2210 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 2328 3719 1870
Size: 73 66 319 1391 4545
Fragments arranged by size:
4545 1391 319 73 66
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2511 2580 3715 5170
Size: 325 217 627 366 358 69 1135 1455
Cuts at: 5170 618
Size: 1842
Fragments arranged by size:
1842 1455 1135 627 366 358 325 217
69
BanII G_rGCy'C
Cuts at: 1391 2906 3108 3386 3465 3713 1391
Size: 1515 202 278 79 248 4072
Fragments arranged by size:
4072 1515 278 248 202 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5423
BbvCI CC'TCA_GC
Cuts at: 2981 3003 2981
Size: 22 6372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4585 4619 1903
Size: 34 50 34 2564 34 3678
Fragments arranged by size:
3678 2564 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2564 4286 5496 2564
Size: 1722 1210 3462
Fragments arranged by size:
3462 1722 1210
BclI T'GATC_A
Cuts at: 1548 2365 3261 3531 1548
Size: 817 896 270 4411
Fragments arranged by size:
4411 896 817 270
BfrBI ATG'CAT
Cuts at: 3235 3235
Size: 6394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 2715 3390
Size: 107 122 71 631 605 526 517 675
Cuts at: 3390 3416 5005 136
Size: 26 1589 1525
Fragments arranged by size:
1589 1525 675 631 605 526 517 122
107 71 26
BglII A'GATC_T
Cuts at: 6389 6389
Size: 6394
BlpI GC'TnA_GC
Cuts at: 3811 3811
Size: 6394
Bme1580I G_kGCm'C
Cuts at: 1790 4455 5611 1790
Size: 2665 1156 2573
Fragments arranged by size:
2665 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2310 2371 3228 1318
Size: 992 61 857 4484
Fragments arranged by size:
4484 992 857 61
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2676 3420 4125
Size: 416 53 83 399 24 1259 744 705
Cuts at: 4125 5083 442
Size: 958 1753
Fragments arranged by size:
1753 1259 958 744 705 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2959 2920
Size: 39 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2929 3605 5054 1239
Size: 1690 676 1449 2579
Fragments arranged by size:
2579 1690 1449 676
Bpu10I CC'TnA_GC
Cuts at: 2202 2919 2981 3003 2202
Size: 717 62 22 5593
Fragments arranged by size:
5593 717 62 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2732 4519 5384 5682 2153
Size: 579 1787 865 298 2865
Fragments arranged by size:
2865 1787 865 579 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2640 2763 3128 3502 5057 882
Size: 617 1141 123 365 374 1555 2219
Fragments arranged by size:
2219 1555 1141 617 374 365 123
BsaAI yAC'GTr
Cuts at: 493 1204 4185 493
Size: 711 2981 2702
Fragments arranged by size:
2981 2702 711
BsaBI GATnn'nnATC
Cuts at: 3674 6388 3674
Size: 2714 3680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 2618 4201 4583
Size: 53 83 186 936 1062 23 1583 382
Cuts at: 4583 5218 275
Size: 635 1451
Fragments arranged by size:
1583 1451 1062 936 635 382 186 83
53 23
BsaWI w'CCGG_w
Cuts at: 2649 3087 4827 5225 5499 5646 6057 2649
Size: 438 1740 398 274 147 411 2986
Fragments arranged by size:
2986 1740 438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 3482 3512 1178
Size: 30 165 30 1092 30 957 30 4060
Fragments arranged by size:
4060 1092 957 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2516 3263 1186
Size: 943 387 747 4317
Fragments arranged by size:
4317 943 747 387
BseYI C'CCAG_C
Cuts at: 2517 3181 3306 3321 3609 5597 6093 2517
Size: 664 125 15 288 1988 496 2818
Fragments arranged by size:
2818 1988 664 496 288 125 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5821
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2911 3844 4605 5633 1540
Size: 275 186 910 933 761 1028 2301
Fragments arranged by size:
2301 1028 933 910 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4455 4540 5611 4455
Size: 85 1071 5238
Fragments arranged by size:
5238 1071 85
BsmI GAATG_Cn'
Cuts at: 2352 3905 3998 2352
Size: 1553 93 4748
Fragments arranged by size:
4748 1553 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 3558 2285
Size: 1273 5121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2906 3108 3386 3465 3713 4455 4540
Size: 399 1116 202 278 79 248 742 85
Cuts at: 4540 5611 1391
Size: 1071 2174
Fragments arranged by size:
2174 1116 1071 742 399 278 248 202
85 79
BspEI T'CCGG_A
Cuts at: 6057 6057
Size: 6394
BspHI T'CATG_A
Cuts at: 4283 6013 4283
Size: 1730 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3749 844
Size: 2905 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2899 4281 1394
Size: 429 1076 1382 3507
Fragments arranged by size:
3507 1382 1076 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4889 5063 65
Size: 1183 3641 174 1396
Fragments arranged by size:
3641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5038 5225 1700
Size: 110 3228 187 2869
Fragments arranged by size:
3228 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6394
BssSI C'ACGA_G
Cuts at: 3032 3131 4454 5466 3032
Size: 99 1323 1012 3960
Fragments arranged by size:
3960 1323 1012 99
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 2048 1184
Size: 864 5530
BstXI CCAn_nnnn'nTGG
Cuts at: 3115 3290 3115
Size: 175 6219
BstYI r'GATC_y
Cuts at: 1972 3724 4091 4476 4493 5945 6031 6043 6389
Size: 1752 367 385 17 1452 86 12 346
Cuts at: 6389 1972
Size: 1977
Fragments arranged by size:
1977 1752 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2140 2773 2830 3052 513
Size: 552 1075 633 57 222 3855
Fragments arranged by size:
3855 1075 633 552 222 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5655
Fragments arranged by size:
5655 683 56
BtsI GCAGTG_nn'
Cuts at: 2887 3687 3910 4704 4724 2887
Size: 800 223 794 20 4557
Fragments arranged by size:
4557 800 794 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2863 2898 529
Size: 35 2299 35 4025
Fragments arranged by size:
4025 2299 35 35
DraI TTT'AAA
Cuts at: 3698 3698
Size: 6394
DraIII CAC_nnn'GTG
Cuts at: 3667 3667
Size: 6394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2962 5401 809
Size: 507 1646 2439 1802
Fragments arranged by size:
2439 1802 1646 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2208 3841
Size: 54 1425 50 275 18 313 65 1633
Cuts at: 3841 4730 6359 8
Size: 889 1629 43
Fragments arranged by size:
1633 1629 1425 889 313 275 65 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3841 1537
Size: 275 2029 4090
Fragments arranged by size:
4090 2029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2790 3052 3114 4324 1353
Size: 692 745 262 62 1210 3423
Fragments arranged by size:
3423 1210 745 692 262 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4981 5355 5501 387
Size: 1064 138 3392 374 146 1280
Fragments arranged by size:
3392 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2929 3167 3332 3605 4457 5054 5841
Size: 393 1297 238 165 273 852 597 787
Cuts at: 5841 6109 1239
Size: 268 1524
Fragments arranged by size:
1524 1297 852 787 597 393 273 268
238 165
EcoO109I rG'GnC_Cy
Cuts at: 2088 2300 2088
Size: 212 6182
EcoRI G'AATT_C
Cuts at: 3703 4081 3703
Size: 378 6016
FspI TGC'GCA
Cuts at: 2735 4900 2735
Size: 2165 4229
HaeII r_GCGC'y
Cuts at: 1678 3328 5541 1678
Size: 1650 2213 2531
Fragments arranged by size:
2531 2213 1650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2350 2382 3253 3285 5035
Size: 32 135 32 778 32 871 32 1750
Cuts at: 5035 5067 5109 5141 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 871 778 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3738 3984 669
Size: 988 281 1800 246 3079
Fragments arranged by size:
3079 1800 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3347 748
Size: 2599 3795
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 2515 3719 2515
Size: 1204 5190
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2931 3167 5508 5692 1305
Size: 1626 236 2341 184 2007
Fragments arranged by size:
2341 2007 1626 236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 2210 10
Size: 54 1425 343 313 65 4194
Fragments arranged by size:
4194 1425 343 313 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2946 3230 3575 4352 4711 4870 518
Size: 648 1780 284 345 777 359 159 2042
Fragments arranged by size:
2042 1780 777 648 359 345 284 159
MspA1I CmG'CkG
Cuts at: 1606 2023 3181 3216 3310 3810 4487 5635 5880
Size: 417 1158 35 94 500 677 1148 245
Cuts at: 5880 1606
Size: 2120
Fragments arranged by size:
2120 1158 1148 677 500 417 245 94
35
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5842
NdeI CA'TA_TG
Cuts at: 387 2854 387
Size: 2467 3927
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NsiI A_TGCA'T
Cuts at: 3237 3237
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 3580 3752 5297 1132
Size: 97 2351 172 1545 2229
Fragments arranged by size:
2351 2229 1545 172 97
PasI CC'CwG_GG
Cuts at: 1348 2790 1348
Size: 1442 4952
PciI A'CATG_T
Cuts at: 5293 5293
Size: 6394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 2790 1108
Size: 1074 608 4712
Fragments arranged by size:
4712 1074 608
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4463 4495 5976 6008 3482
Size: 32 949 32 1481 32 3868
Fragments arranged by size:
3868 1481 949 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 2300
Size: 6394
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6394
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PstI C_TGCA'G
Cuts at: 830 3043 3474 3746 830
Size: 2213 431 272 3478
Fragments arranged by size:
3478 2213 431 272
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
PvuII CAG'CTG
Cuts at: 3181 3216 3310 3181
Size: 35 94 6265
Fragments arranged by size:
6265 94 35
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SapI GCTCTTCn'nnn_
Cuts at: 3114 3114
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3746 3746
Size: 6394
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SexAI A'CCwGG_T
Cuts at: 2601 3492 2601
Size: 891 5503
SfcI C'TryA_G
Cuts at: 826 1045 2719 3039 3176 3470 3742 4877 5558
Size: 219 1674 320 137 294 272 1135 681
Cuts at: 5558 5749 826
Size: 191 1471
Fragments arranged by size:
1674 1471 1135 681 320 294 272 219
191 137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 3721 1872
Size: 1849 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2711 4498 5399 5661 820
Size: 197 926 225 543 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 543 262 225 197
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3580 3752 1132
Size: 2448 172 3774
Fragments arranged by size:
3774 2448 172
SspI AAT'ATT
Cuts at: 5 52 4318 5
Size: 47 4266 2081
Fragments arranged by size:
4266 2081 47
StuI AGG'CCT
Cuts at: 1517 2134 1517
Size: 617 5777
StyI C'CwwG_G
Cuts at: 513 1065 2454 2874 2958 3555 3833 513
Size: 552 1389 420 84 597 278 3074
Fragments arranged by size:
3074 1389 597 552 420 278 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 2279 2447 2658 3428 4421 4438
Size: 1255 695 4 168 211 770 993 17
Cuts at: 4438 4591 4776 5221 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 993 770 695 445 211 185
168 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2634 4640 6352
Size: 274 80 33 51 1443 657 2006 1712
Cuts at: 6352 96
Size: 138
Fragments arranged by size:
2006 1712 1443 657 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6354 6386 6354
Size: 32 6362
Tth111I GACn'n_nGTC
Cuts at: 1316 3356 1316
Size: 2040 4354
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 3719 1870
Size: 1849 4545
XmnI GAAnn'nnTTC
Cuts at: 2818 4523 2818
Size: 1705 4689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI