Restriction map for
pFN21AE5685
(Circular) (Six-base) MAPSORT of: pFN21AE5685.seq Check: 441 from: 1 to: 6388
pFN21AE5685 6388 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2077 3199 2077
Size: 1122 5266
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2560 2806 3101 4451 5835 6103 1632
Size: 597 331 246 295 1350 1384 268 1917
Fragments arranged by size:
1917 1384 1350 597 331 295 268 246
AfeI AGC'GCT
Cuts at: 3457 3457
Size: 6388
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 2081 5287 6264 1203
Size: 878 3206 977 1327
Fragments arranged by size:
3206 1327 977 878
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3221 1184
Size: 2037 4351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3665 3697 3665
Size: 32 6356
AlwNI CAG_nnn'CTG
Cuts at: 2978 4131 5703 2978
Size: 1153 1572 3663
Fragments arranged by size:
3663 1572 1153
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2175 3697 3923 4075 6184 1493
Size: 682 1522 226 152 2109 1697
Fragments arranged by size:
2109 1697 1522 682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 3713 1870
Size: 73 1770 4545
Fragments arranged by size:
4545 1770 73
AvrII C'CTAG_G
Cuts at: 2419 2419
Size: 6388
BamHI G'GATC_C
Cuts at: 2452 3718 4085 2452
Size: 1266 367 4755
Fragments arranged by size:
4755 1266 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3709 5164 618
Size: 325 217 627 1922 1455 1842
Fragments arranged by size:
1922 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3146 3254 3707 1391
Size: 1755 108 453 4072
Fragments arranged by size:
4072 1755 453 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3076 928
Size: 971 1177 4240
Fragments arranged by size:
4240 1177 971
BbvCI CC'TCA_GC
Cuts at: 2359 3318 2359
Size: 959 5429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3405 4280 5490 3405
Size: 875 1210 4303
Fragments arranged by size:
4303 1210 875
BclI T'GATC_A
Cuts at: 1548 2182 1548
Size: 634 5754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3150 4999 136
Size: 107 122 71 631 605 1478 1849 1525
Fragments arranged by size:
1849 1525 1478 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 3140 3805 3140
Size: 665 5723
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6388
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 4119 5077
Size: 416 53 83 399 24 1000 1702 958
Cuts at: 5077 442
Size: 1753
Fragments arranged by size:
1753 1702 1000 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3273 3273
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 2908 5048 1239
Size: 1117 552 2140 2579
Fragments arranged by size:
2579 2140 1117 552
Bpu10I CC'TnA_GC
Cuts at: 2359 3318 2359
Size: 959 5429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2455 4513 5378 5676 2455
Size: 2058 865 298 3167
Fragments arranged by size:
3167 2058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2664 2700 5051 882
Size: 617 1165 36 2351 2219
Fragments arranged by size:
2351 2219 1165 617 36
BsaAI yAC'GTr
Cuts at: 493 1204 2082 4179 493
Size: 711 878 2097 2702
Fragments arranged by size:
2702 2097 878 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3246 4821 5219 5493 5640 6051 3246
Size: 1575 398 274 147 411 3583
Fragments arranged by size:
3583 1575 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2763 2793 1178
Size: 30 165 30 1360 30 4773
Fragments arranged by size:
4773 1360 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 1186
Size: 1603 4785
BseYI C'CCAG_C
Cuts at: 2049 2322 3272 5591 6087 2049
Size: 273 950 2319 496 2350
Fragments arranged by size:
2350 2319 950 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 2831 3227 1163
Size: 573 401 694 396 4324
Fragments arranged by size:
4324 694 573 401 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2342 3298 4449 4534 5605 2342
Size: 956 1151 85 1071 3125
Fragments arranged by size:
3125 1151 1071 956 85
BsmI GAATG_Cn'
Cuts at: 3312 3899 3992 3312
Size: 587 93 5708
Fragments arranged by size:
5708 587 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2342 3146 3254 3298 3707 4449 4534
Size: 399 552 804 108 44 409 742 85
Cuts at: 4534 5605 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 804 742 552 409 399 108
85 44
BspEI T'CCGG_A
Cuts at: 3246 6051 3246
Size: 2805 3583
BspHI T'CATG_A
Cuts at: 2179 3130 4277 6007 2179
Size: 951 1147 1730 2560
Fragments arranged by size:
2560 1730 1147 951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2077 3199 3743 844
Size: 1233 1122 544 3489
Fragments arranged by size:
3489 1233 1122 544
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5032 5219 1700
Size: 110 3222 187 2869
Fragments arranged by size:
3222 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3307 794
Size: 2513 3875
BstBI TT'CG_AA
Cuts at: 2104 3087 5192 2104
Size: 983 2105 3300
Fragments arranged by size:
3300 2105 983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 2452 3718 4085 4470 4487 5939 6025 6037
Size: 480 1266 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1266 480 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2263 513
Size: 552 939 259 4638
Fragments arranged by size:
4638 939 552 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 3904 4698 4718 3904
Size: 794 20 5574
Fragments arranged by size:
5574 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 2679 3692 2679
Size: 1013 5375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2765 2783 2893
Size: 54 1425 50 275 18 935 18 110
Cuts at: 2893 3835 4724 6353 8
Size: 942 889 1629 43
Fragments arranged by size:
1629 1425 942 935 889 275 110 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2356 2560 2806 2908 3101 4451
Size: 393 597 127 204 246 102 193 1350
Cuts at: 4451 5048 5835 6103 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1350 787 597 597 393 268 246
204 193 127 102
EcoNI CCTnn'n_nnAGG
Cuts at: 3063 3063
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 2794 3322 3634 2794
Size: 528 312 5548
Fragments arranged by size:
5548 528 312
EcoRI G'AATT_C
Cuts at: 2175 3697 4075 2175
Size: 1522 378 4488
Fragments arranged by size:
4488 1522 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2738 2738
Size: 6388
FspI TGC'GCA
Cuts at: 2250 4894 2250
Size: 2644 3744
HaeII r_GCGC'y
Cuts at: 1678 3459 5535 1678
Size: 1781 2076 2531
Fragments arranged by size:
2531 2076 1781
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2525 2557 2582 2614 3564
Size: 32 135 32 953 32 25 32 950
Cuts at: 3564 3596 5029 5061 5103 5135 1373
Size: 32 1433 32 42 32 2626
Fragments arranged by size:
2626 1433 953 950 135 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2989 3732 3978 669
Size: 988 281 1051 743 246 3079
Fragments arranged by size:
3079 1051 988 743 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 2989 3978 2989
Size: 989 5399
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5502 5686 1305
Size: 4197 184 2007
Fragments arranged by size:
4197 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2767 2785 2895 10
Size: 54 1425 343 935 18 110 3503
Fragments arranged by size:
3503 1425 935 343 110 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 4346 4705 4864 518
Size: 648 3180 359 159 2042
Fragments arranged by size:
3180 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2864 3139 3804 4481 5629 5874 1606
Size: 411 847 275 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 677 665 411 275 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4897
Fragments arranged by size:
4897 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 2395 3746 5291 1132
Size: 97 1166 1351 1545 2229
Fragments arranged by size:
2229 1545 1351 1166 97
PasI CC'CwG_GG
Cuts at: 1348 3219 1348
Size: 1871 4517
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6388
PfoI T'CCnGG_A
Cuts at: 2199 2529 2199
Size: 330 6058
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 2082 2082
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2794
Size: 6388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 6356
PstI C_TGCA'G
Cuts at: 830 2304 2731 2863 3545 3740 830
Size: 1474 427 132 682 195 3478
Fragments arranged by size:
3478 1474 682 427 195 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2864 2864
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2300 2727 2859 3541 3736 4871 5552
Size: 219 1255 427 132 682 195 1135 681
Cuts at: 5552 5743 826
Size: 191 1471
Fragments arranged by size:
1471 1255 1135 682 681 427 219 195
191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2434 4492 5393 5655 820
Size: 197 926 491 2058 901 262 1553
Fragments arranged by size:
2058 1553 926 901 491 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 2395 3746 1132
Size: 1263 1351 3774
Fragments arranged by size:
3774 1351 1263
SspI AAT'ATT
Cuts at: 5 52 3164 4312 5
Size: 47 3112 1148 2081
Fragments arranged by size:
3112 2081 1148 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2004 2293 2419 2458 2960 3827 513
Size: 552 939 289 126 39 502 867 3074
Fragments arranged by size:
3074 939 867 552 502 289 126 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2343 2357 4415 4432 4585 4770 5215
Size: 1255 763 14 2058 17 153 185 445
Cuts at: 5215 325
Size: 1498
Fragments arranged by size:
2058 1498 1255 763 445 185 153 17
14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4634 6346 96
Size: 274 80 33 51 1443 2657 1712 138
Fragments arranged by size:
2657 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 6348 6380 2763
Size: 32 3553 32 2771
Fragments arranged by size:
3553 2771 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI