Restriction map for
pFN21AE5681
(Circular) (Six-base) MAPSORT of: pFN21AE5681.seq Check: 8634 from: 1 to: 6271
pFN21AE5681 6271 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2700 3614 1937
Size: 763 914 4594
Fragments arranged by size:
4594 914 763
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 3273 4398 4771 1563
Size: 1710 1125 373 3063
Fragments arranged by size:
3063 1710 1125 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 2480 2618 3223 4334 5718 5986 1632
Size: 776 72 138 605 1111 1384 268 1917
Fragments arranged by size:
1917 1384 1111 776 605 268 138 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 3465 5170 6147 1203
Size: 2262 1705 977 1327
Fragments arranged by size:
2262 1705 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3012 1184
Size: 1828 4443
AlwNI CAG_nnn'CTG
Cuts at: 2374 2553 4014 5586 2374
Size: 179 1461 1572 3059
Fragments arranged by size:
3059 1572 1461 179
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2176 2582 3580 3806 3958 6067 1493
Size: 683 406 998 226 152 2109 1697
Fragments arranged by size:
2109 1697 998 683 406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2592 4825 160
Size: 872 1560 2233 1606
Fragments arranged by size:
2233 1606 1560 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2141 3188 3596 1870
Size: 73 134 64 1047 408 4545
Fragments arranged by size:
4545 1047 408 134 73 64
BamHI G'GATC_C
Cuts at: 2486 3601 3968 2486
Size: 1115 367 4789
Fragments arranged by size:
4789 1115 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2670 2744 3493 3592
Size: 325 217 627 294 589 74 749 99
Cuts at: 3592 5047 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 749 627 589 325 294 217
99 74
BanII G_rGCy'C
Cuts at: 1391 2160 2817 3590 1391
Size: 769 657 773 4072
Fragments arranged by size:
4072 773 769 657
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 6271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2606 2845 3309 928
Size: 971 707 239 464 3890
Fragments arranged by size:
3890 971 707 464 239
BbvCI CC'TCA_GC
Cuts at: 3365 3398 3365
Size: 33 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2398 2432 4462 4496 1903
Size: 34 461 34 2030 34 3678
Fragments arranged by size:
3678 2030 461 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4882 136
Size: 107 122 71 631 605 337 2873 1525
Fragments arranged by size:
2873 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2625 6266 2625
Size: 3641 2630
BlpI GC'TnA_GC
Cuts at: 2054 3688 2054
Size: 1634 4637
Bme1580I G_kGCm'C
Cuts at: 1790 2747 2991 3496 4332 5488 1790
Size: 957 244 505 836 1156 2573
Fragments arranged by size:
2573 1156 957 836 505 244
BmgBI CAC'GTC
Cuts at: 1318 2833 2862 2992 1318
Size: 1515 29 130 4597
Fragments arranged by size:
4597 1515 130 29
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 2563 2701
Size: 416 53 83 399 24 1023 123 138
Cuts at: 2701 3484 3499 4002 4960 442
Size: 783 15 503 958 1753
Fragments arranged by size:
1753 1023 958 783 503 416 399 138
123 83 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2146 2831 3519 4931 1239
Size: 907 685 688 1412 2579
Fragments arranged by size:
2579 1412 907 688 685
Bpu10I CC'TnA_GC
Cuts at: 3365 3398 3365
Size: 33 6238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4396 5261 5559 4396
Size: 865 298 5108
Fragments arranged by size:
5108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 4934 882
Size: 617 663 2772 2219
Fragments arranged by size:
2772 2219 663 617
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2703 2772 3071
Size: 53 83 186 936 549 621 69 299
Cuts at: 3071 4078 4460 5095 275
Size: 1007 382 635 1451
Fragments arranged by size:
1451 1007 936 635 621 549 382 299
186 83 69 53
BsaWI w'CCGG_w
Cuts at: 2184 4704 5102 5376 5523 5934 2184
Size: 2520 398 274 147 411 2521
Fragments arranged by size:
2521 2520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2760 2790 2799 2829 1178
Size: 30 165 30 1357 30 9 30 4620
Fragments arranged by size:
4620 1357 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 3098 1186
Size: 1621 291 4359
Fragments arranged by size:
4359 1621 291
BseYI C'CCAG_C
Cuts at: 3019 3077 3456 5474 5970 3019
Size: 58 379 2018 496 3320
Fragments arranged by size:
3320 2018 496 379 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2813 1163
Size: 573 1077 4621
Fragments arranged by size:
4621 1077 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3417 3721 4482 5510 1540
Size: 275 186 1416 304 761 1028 2301
Fragments arranged by size:
2301 1416 1028 761 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 2243 2817 4332 4417 5488 2055
Size: 188 574 1515 85 1071 2838
Fragments arranged by size:
2838 1515 1071 574 188 85
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
BsmBI CGTCTCn'nnnn_
Cuts at: 2763 2763
Size: 6271
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2160 2243 2747 2817 2991 3496
Size: 399 265 105 83 504 70 174 505
Cuts at: 3496 3590 4332 4417 5488 1391
Size: 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 505 504 399 265 174
105 94 85 83 70
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 3483 4160 5890 3483
Size: 677 1730 3864
Fragments arranged by size:
3864 1730 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2640 2835 3626 844
Size: 1796 195 791 3489
Fragments arranged by size:
3489 1796 791 195
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 4766 4940 65
Size: 1183 1045 2473 174 1396
Fragments arranged by size:
2473 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2396 3129 4915 5102 1700
Size: 110 586 733 1786 187 2869
Fragments arranged by size:
2869 1786 733 586 187 110
BsrGI T'GTAC_A
Cuts at: 96 2544 96
Size: 2448 3823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2044 2374 794
Size: 1250 330 4691
Fragments arranged by size:
4691 1250 330
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 2135 1184
Size: 951 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 3052 3052
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2486 2625 3601 3968 4353 4370 5822 5908
Size: 514 139 976 367 385 17 1452 86
Cuts at: 5908 5920 6266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 976 514 385 367 346 139
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 3160 513
Size: 552 939 57 1099 3624
Fragments arranged by size:
3624 1099 939 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2994 3787 4581 4601 2258
Size: 736 793 794 20 3928
Fragments arranged by size:
3928 794 793 736 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2831 5278 809
Size: 507 1515 2447 1802
Fragments arranged by size:
2447 1802 1515 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2981 3180 3718
Size: 54 1425 50 275 18 1151 199 538
Cuts at: 3718 4607 6236 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1151 889 538 275 199 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3044 4201 1353
Size: 1691 1157 3423
Fragments arranged by size:
3423 1691 1157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2878 4858 5232 5378 387
Size: 1064 138 1289 1980 374 146 1280
Fragments arranged by size:
1980 1289 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2815 2815
Size: 6271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2408 2480 2618 2831 3223 3519
Size: 393 514 262 72 138 213 392 296
Cuts at: 3519 4334 4931 5718 5986 1239
Size: 815 597 787 268 1524
Fragments arranged by size:
1524 815 787 597 514 393 392 296
268 262 213 138 72
EcoNI CCTnn'n_nnAGG
Cuts at: 3122 3122
Size: 6271
EcoO109I rG'GnC_Cy
Cuts at: 2073 2442 3343 3394 2073
Size: 369 901 51 4950
Fragments arranged by size:
4950 901 369 51
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2222 3223 2222
Size: 1001 5270
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2061 2085 2877 3381 5418 1678
Size: 383 24 792 504 2037 2531
Fragments arranged by size:
2531 2037 792 504 383 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2760 2792 4912 4944 4986
Size: 32 135 32 1188 32 2120 32 42
Cuts at: 4986 5018 1373
Size: 32 2626
Fragments arranged by size:
2626 2120 1188 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2701 3615 3861 669
Size: 988 281 763 914 246 3079
Fragments arranged by size:
3079 988 914 763 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 5385 5569 1305
Size: 1539 2541 184 2007
Fragments arranged by size:
2541 2007 1539 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2983 10
Size: 54 1425 343 1151 3298
Fragments arranged by size:
3298 1425 1151 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3173 4229 4588 4747 518
Size: 648 1374 633 1056 359 159 2042
Fragments arranged by size:
2042 1374 1056 648 633 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2063 3019 3687 4364 5512 5757 1606
Size: 435 22 956 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 668 435 245 22
NaeI GCC'GGC
Cuts at: 2398 3131 2398
Size: 733 5538
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4780
Fragments arranged by size:
4780 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NgoMIV G'CCGG_C
Cuts at: 2396 3129 2396
Size: 733 5538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 3629 5174 1132
Size: 97 2400 1545 2229
Fragments arranged by size:
2400 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3263 3347 1348
Size: 1915 84 4272
Fragments arranged by size:
4272 1915 84
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3019 3228 1108
Size: 1911 209 4151
Fragments arranged by size:
4151 1911 209
PfoI T'CCnGG_A
Cuts at: 2778 2778
Size: 6271
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3343 3343
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2197 2637 3001 3126 3623 830
Size: 1367 440 364 125 497 3478
Fragments arranged by size:
3478 1367 497 440 364 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2041 3019 2041
Size: 978 5293
SacI G_AGCT'C
Cuts at: 2817 2817
Size: 6271
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 2699 3613 1936
Size: 763 914 4594
Fragments arranged by size:
4594 914 763
SanDI GG'GwC_CC
Cuts at: 3343 3343
Size: 6271
SbfI CC_TGCA'GG
Cuts at: 2637 3126 3623 2637
Size: 489 497 5285
Fragments arranged by size:
5285 497 489
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2193 2633 2997 3122 3619 4754 5435
Size: 219 1148 440 364 125 497 1135 681
Cuts at: 5435 5626 826
Size: 191 1471
Fragments arranged by size:
1471 1148 1135 681 497 440 364 219
191 125
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 6271
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4375 5276 5538 820
Size: 197 926 2432 901 262 1553
Fragments arranged by size:
2432 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 3145 3437 1517
Size: 1628 292 4351
Fragments arranged by size:
4351 1628 292
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 3710 513
Size: 552 939 1434 272 3074
Fragments arranged by size:
3074 1434 939 552 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2636 3403 4298 4315 4468 4653 5098
Size: 1255 1056 767 895 17 153 185 445
Cuts at: 5098 325
Size: 1498
Fragments arranged by size:
1498 1255 1056 895 767 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 4517 6229
Size: 274 80 33 51 1443 567 1973 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
1973 1712 1443 567 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 2774 1316
Size: 1458 4813
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2737
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 3543 4400 2583
Size: 960 857 4454
Fragments arranged by size:
4454 960 857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SapI SexAI SfiI SrfI SwaI