Restriction map for
pFN21AE5680
(Circular) (Six-base) MAPSORT of: pFN21AE5680.seq Check: 5012 from: 1 to: 6262
pFN21AE5680 6262 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3171 3605 1937
Size: 1234 434 4594
Fragments arranged by size:
4594 1234 434
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 3401 4325 5709 5977 1632
Size: 563 1206 924 1384 268 1917
Fragments arranged by size:
1917 1384 1206 924 563 268
AfeI AGC'GCT
Cuts at: 3027 3027
Size: 6262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2925 3107 5161 6138 1203
Size: 1722 182 2054 977 1327
Fragments arranged by size:
2054 1722 1327 977 182
AgeI A'CCGG_T
Cuts at: 2238 3446 5093 2238
Size: 1208 1647 3407
Fragments arranged by size:
3407 1647 1208
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AlwNI CAG_nnn'CTG
Cuts at: 2083 2274 3082 4005 5577 2083
Size: 191 808 923 1572 2768
Fragments arranged by size:
2768 1572 923 808 191
ApaI G_GGCC'C
Cuts at: 2886 3539 2886
Size: 653 5609
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 3571 3797 3949 6058 1493
Size: 2078 226 152 2109 1697
Fragments arranged by size:
2109 2078 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 3587 1870
Size: 73 717 927 4545
Fragments arranged by size:
4545 927 717 73
BamHI G'GATC_C
Cuts at: 3330 3592 3959 3330
Size: 262 367 5633
Fragments arranged by size:
5633 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 3277 3583 5038 618
Size: 325 217 627 360 1130 306 1455 1842
Fragments arranged by size:
1842 1455 1130 627 360 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2354 2886 2952 2984 3090 3396 3504 3539
Size: 963 532 66 32 106 306 108 35
Cuts at: 3539 3581 1391
Size: 42 4072
Fragments arranged by size:
4072 963 532 306 108 106 66 42
35 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2662 928
Size: 971 763 4528
Fragments arranged by size:
4528 971 763
BbvCI CC'TCA_GC
Cuts at: 2501 3311 2501
Size: 810 5452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4453 4487 1903
Size: 34 50 34 2432 34 3678
Fragments arranged by size:
3678 2432 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 2190 1548
Size: 642 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3350 4873 136
Size: 107 122 71 631 605 1678 1523 1525
Fragments arranged by size:
1678 1525 1523 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 2249 3679 2249
Size: 1430 4832
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2886 3282 3539 4323 5479 1790
Size: 362 734 396 257 784 1156 2573
Fragments arranged by size:
2573 1156 784 734 396 362 257
BmgBI CAC'GTC
Cuts at: 1318 3357 1318
Size: 2039 4223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2159 2461 3993
Size: 416 53 83 399 24 742 302 1532
Cuts at: 3993 4951 442
Size: 958 1753
Fragments arranged by size:
1753 1532 958 742 416 399 302 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2245 2495 2531 2540 2849 3011 3224 3410
Size: 1006 250 36 9 309 162 213 186
Cuts at: 3410 3551 4922 1239
Size: 141 1371 2579
Fragments arranged by size:
2579 1371 1006 309 250 213 186 162
141 36 9
Bpu10I CC'TnA_GC
Cuts at: 2258 2501 3311 3323 2258
Size: 243 810 12 5197
Fragments arranged by size:
5197 810 243 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3380 4387 5252 5550 3380
Size: 1007 865 298 4092
Fragments arranged by size:
4092 1007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 2795 4925 882
Size: 617 1065 231 2130 2219
Fragments arranged by size:
2219 2130 1065 617 231
BsaAI yAC'GTr
Cuts at: 493 1204 2926 3043 3110 3175 4053 493
Size: 711 1722 117 67 65 878 2702
Fragments arranged by size:
2702 1722 878 711 117 67 65
BsaBI GATnn'nnATC
Cuts at: 2189 3335 6256 2189
Size: 1146 2921 2195
Fragments arranged by size:
2921 2195 1146
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2157 4069 4451
Size: 53 83 186 936 569 55 1912 382
Cuts at: 4451 5086 275
Size: 635 1451
Fragments arranged by size:
1912 1451 936 635 569 382 186 83
55 53
BsaWI w'CCGG_w
Cuts at: 2127 2238 3446 4695 5093 5367 5514 5925 2127
Size: 111 1208 1249 398 274 147 411 2464
Fragments arranged by size:
2464 1249 1208 411 398 274 147 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 3359 3389 1178
Size: 30 165 30 687 30 1239 30 4051
Fragments arranged by size:
4051 1239 687 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2303 2453 2477 2528 2537 2584 2765
Size: 1021 96 150 24 51 9 47 181
Cuts at: 2765 2960 1186
Size: 195 4488
Fragments arranged by size:
4488 1021 195 181 150 96 51 47
24 9
BseYI C'CCAG_C
Cuts at: 2354 2601 3067 3082 3538 5465 5961 2354
Size: 247 466 15 456 1927 496 2655
Fragments arranged by size:
2655 1927 496 466 456 247 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2522 1163
Size: 573 582 204 4903
Fragments arranged by size:
4903 582 573 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2391 3712 4473 5501 1540
Size: 275 186 390 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 390 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 2984 3396 3504 4323 4408 5479 2231
Size: 753 412 108 819 85 1071 3014
Fragments arranged by size:
3014 1071 819 753 412 108 85
BsmI GAATG_Cn'
Cuts at: 2771 3773 3866 2771
Size: 1002 93 5167
Fragments arranged by size:
5167 1002 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2093 2016
Size: 77 6185
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2231 2354 2886 2952 2984 3090
Size: 399 362 79 123 532 66 32 106
Cuts at: 3090 3282 3396 3504 3539 3581 4323 4408 5479
Size: 192 114 108 35 42 742 85 1071
Cuts at: 5479 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 532 399 362 192 123
114 108 106 85 79 66 42 35
32
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 3504 4151 5881 3504
Size: 647 1730 3885
Fragments arranged by size:
3885 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2976 3327 4149 1394
Size: 429 1153 351 822 3507
Fragments arranged by size:
3507 1153 822 429 351
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2619 3446 4906 5093 1700
Size: 110 428 381 827 1460 187 2869
Fragments arranged by size:
2869 1460 827 428 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 2421 1925
Size: 496 5766
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2054 2095 2812 2054
Size: 41 717 5504
Fragments arranged by size:
5504 717 41
BstYI r'GATC_y
Cuts at: 1972 2184 3330 3592 3959 4344 4361 5813 5899
Size: 212 1146 262 367 385 17 1452 86
Cuts at: 5899 5911 6257 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 262 212
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2741 2741
Size: 6262
BtgI C'CryG_G
Cuts at: 513 1065 2094 3141 513
Size: 552 1029 1047 3634
Fragments arranged by size:
3634 1047 1029 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 487
Size: 683 56 1096 4427
Fragments arranged by size:
4427 1096 683 56
BtsI GCAGTG_nn'
Cuts at: 3778 4572 4592 3778
Size: 794 20 5448
Fragments arranged by size:
5448 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2051 2086 529
Size: 35 1487 35 4705
Fragments arranged by size:
4705 1487 35 35
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DraIII CAC_nnn'GTG
Cuts at: 3289 3289
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 2529 2697
Size: 54 1425 50 275 18 558 141 168
Cuts at: 2697 2907 3138 3144 3177 3351 3486 3709 4598
Size: 210 231 6 33 174 135 223 889
Cuts at: 4598 6227 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 558 275 231 223 210
174 168 141 135 54 50 43 33
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2388 3709 1537
Size: 275 576 1321 4090
Fragments arranged by size:
4090 1321 576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2696 3137 3161 4192 1353
Size: 1343 441 24 1031 3423
Fragments arranged by size:
3423 1343 1031 441 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3419 4849 5223 5369 387
Size: 1064 138 1830 1430 374 146 1280
Fragments arranged by size:
1830 1430 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2982 3394 3502 2982
Size: 412 108 5742
Fragments arranged by size:
5742 412 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2245 2495 2531 2540 2849 3011
Size: 393 563 50 250 36 9 309 162
Cuts at: 3011 3224 3401 3410 3551 4325 4922 5709 5977
Size: 213 177 9 141 774 597 787 268
Cuts at: 5977 1239
Size: 1524
Fragments arranged by size:
1524 787 774 597 563 393 309 268
250 213 177 162 141 50 36 9
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2993 2177
Size: 816 5446
EcoO109I rG'GnC_Cy
Cuts at: 2029 2116 2605 2882 2883 3535 2029
Size: 87 489 277 1 652 4756
Fragments arranged by size:
4756 652 489 277 87 1
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FspI TGC'GCA
Cuts at: 3032 4768 3032
Size: 1736 4526
HaeII r_GCGC'y
Cuts at: 1678 2361 3029 5409 1678
Size: 683 668 2380 2531
Fragments arranged by size:
2531 2380 683 668
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2090 2122 3173 3205 4903
Size: 32 135 32 518 32 1051 32 1698
Cuts at: 4903 4935 4977 5009 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1698 1051 518 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2258 5376 5560 1305
Size: 953 3118 184 2007
Fragments arranged by size:
3118 2007 953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 2699 2909 3146 3488
Size: 54 1425 343 699 168 210 237 342
Cuts at: 3488 10
Size: 2784
Fragments arranged by size:
2784 1425 699 343 342 237 210 168
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2052 2810 3248 3557 4220 4579
Size: 648 843 43 758 438 309 663 359
Cuts at: 4579 4738 518
Size: 159 2042
Fragments arranged by size:
2042 843 758 663 648 438 359 309
159 43
MspA1I CmG'CkG
Cuts at: 1606 2248 3678 4355 5503 5748 1606
Size: 642 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 642 245
NcoI C'CATG_G
Cuts at: 513 1065 2094 513
Size: 552 1029 4681
Fragments arranged by size:
4681 1029 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3620 5165 1132
Size: 97 2391 1545 2229
Fragments arranged by size:
2391 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3067 3304 1108
Size: 1959 237 4066
Fragments arranged by size:
4066 1959 237
PfoI T'CCnGG_A
Cuts at: 2433 3471 3528 2433
Size: 1038 57 5167
Fragments arranged by size:
5167 1038 57
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PmlI CAC'GTG
Cuts at: 3110 3110
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3486 3518 4331 4363 5844 5876 3486
Size: 32 813 32 1481 32 3872
Fragments arranged by size:
3872 1481 813 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2865 1613
Size: 1252 5010
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 2882 3535 2882
Size: 653 5609
PstI C_TGCA'G
Cuts at: 830 2412 3078 3614 830
Size: 1582 666 536 3478
Fragments arranged by size:
3478 1582 666 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
SacI G_AGCT'C
Cuts at: 2984 3396 3504 2984
Size: 412 108 5742
Fragments arranged by size:
5742 412 108
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SfcI C'TryA_G
Cuts at: 826 1045 2408 3074 3610 4745 5426 5617 826
Size: 219 1363 666 536 1135 681 191 1471
Fragments arranged by size:
1471 1363 1135 681 666 536 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3350 3350
Size: 6262
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 2662 3589 1872
Size: 790 927 4545
Fragments arranged by size:
4545 927 790
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 4366 5267 5529 820
Size: 197 926 1452 971 901 262 1553
Fragments arranged by size:
1553 1452 971 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2069 2094 3701 513
Size: 552 1004 25 1607 3074
Fragments arranged by size:
3074 1607 1004 552 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 4289 4306 4459 4644 5089 325
Size: 1255 555 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 555 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4508 6220 96
Size: 274 80 33 51 1443 2531 1712 138
Fragments arranged by size:
2531 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2131 2163 3359 3391 6222 6254 2042
Size: 32 57 32 1196 32 2831 32 2050
Fragments arranged by size:
2831 2050 1196 57 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 5474
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2692 3256 2692
Size: 564 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 2660 3587 1870
Size: 790 927 4545
Fragments arranged by size:
4545 927 790
XmnI GAAnn'nnTTC
Cuts at: 2669 4391 2669
Size: 1722 4540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI
SwaI