Restriction map for
pFN21AE5679
(Circular) (Six-base) MAPSORT of: pFN21AE5679.seq Check: 4118 from: 1 to: 6214
pFN21AE5679 6214 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2804 3368 4277 5661 5929 1632
Size: 1172 564 909 1384 268 1917
Fragments arranged by size:
1917 1384 1172 909 564 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2524 5113 6090 1203
Size: 1321 2589 977 1327
Fragments arranged by size:
2589 1327 1321 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 3236 3327 3957 5529 3236
Size: 91 630 1572 3921
Fragments arranged by size:
3921 1572 630 91
ApaI G_GGCC'C
Cuts at: 2127 2127
Size: 6214
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 2115 2842 3523 3749 3901 6010 1493
Size: 622 727 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 727 681 622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3539 1870
Size: 73 1596 4545
Fragments arranged by size:
4545 1596 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 3535 4990 618
Size: 325 217 627 1244 504 1455 1842
Fragments arranged by size:
1842 1455 1244 627 504 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 2889 3533 1391
Size: 736 762 644 4072
Fragments arranged by size:
4072 762 736 644
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 928
Size: 971 400 4843
Fragments arranged by size:
4843 971 400
BbvCI CC'TCA_GC
Cuts at: 2367 2367
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 2271 2553 1548
Size: 723 282 5209
Fragments arranged by size:
5209 723 282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2329 4825 136
Size: 107 122 71 631 605 657 2496 1525
Fragments arranged by size:
2496 1525 657 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2127 4275 5431 1790
Size: 337 2148 1156 2573
Fragments arranged by size:
2573 2148 1156 337
BmgBI CAC'GTC
Cuts at: 1318 2527 1318
Size: 1209 5005
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2449 3101 3945
Size: 416 53 83 399 24 1032 652 844
Cuts at: 3945 4903 442
Size: 958 1753
Fragments arranged by size:
1753 1032 958 844 652 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3363 3363
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 4874 1239
Size: 1664 1971 2579
Fragments arranged by size:
2579 1971 1664
Bpu10I CC'TnA_GC
Cuts at: 2367 2367
Size: 6214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3143 4339 5204 5502 2414
Size: 729 1196 865 298 3126
Fragments arranged by size:
3126 1196 865 729 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2852 3491 4877 882
Size: 617 1353 639 1386 2219
Fragments arranged by size:
2219 1386 1353 639 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2316 4647 5045 5319 5466 5877 2316
Size: 2331 398 274 147 411 2653
Fragments arranged by size:
2653 2331 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 5343
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3017 1163
Size: 573 1281 4360
Fragments arranged by size:
4360 1281 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2889 4275 4360 5431 2889
Size: 1386 85 1071 3672
Fragments arranged by size:
3672 1386 1071 85
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2889 3533 4275 4360 5431 1391
Size: 399 337 762 644 742 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 644 399 337 85
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 3211 4103 5833 3211
Size: 892 1730 3592
Fragments arranged by size:
3592 1730 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3569 844
Size: 2725 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 2448 4709 4883 65
Size: 1183 1148 52 2261 174 1396
Fragments arranged by size:
2261 1396 1183 1148 174 52
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 2932 3350 4274 5286 2932
Size: 418 924 1012 3860
Fragments arranged by size:
3860 1012 924 418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2995 794
Size: 2201 4013
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 3178 3225 3178
Size: 47 6167
BstYI r'GATC_y
Cuts at: 1972 2008 2260 2653 3286 3420 3544 3911 4296
Size: 36 252 393 633 134 124 367 385
Cuts at: 4296 4313 5765 5851 5863 6209 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 633 393 385 367 346 252
134 124 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 513
Size: 552 939 24 4699
Fragments arranged by size:
4699 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3730 4524 4544 2944
Size: 786 794 20 4614
Fragments arranged by size:
4614 794 786 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2809 2809
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2967 3661
Size: 54 1425 50 275 18 195 942 694
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 942 889 694 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 4144 1353
Size: 1025 1766 3423
Fragments arranged by size:
3423 1766 1025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2887 2887
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2804 2903 3368 4277 4874 5661 5929
Size: 393 1172 99 465 909 597 787 268
Cuts at: 5929 1239
Size: 1524
Fragments arranged by size:
1524 1172 909 787 597 465 393 268
99
EcoO109I rG'GnC_Cy
Cuts at: 2123 2535 2123
Size: 412 5802
EcoRI G'AATT_C
Cuts at: 2115 2842 3523 3901 2115
Size: 727 681 378 4428
Fragments arranged by size:
4428 727 681 378
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2565 2597 2821 2853 4855
Size: 32 135 32 993 32 224 32 2002
Cuts at: 4855 4887 4929 4961 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2002 993 224 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2237 3558 3804 669
Size: 988 281 299 1321 246 3079
Fragments arranged by size:
3079 1321 988 299 281 246
HindIII A'AGCT_T
Cuts at: 748 2108 2630 748
Size: 1360 522 4332
Fragments arranged by size:
4332 1360 522
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2432 3042 5328 5512 1305
Size: 1127 610 2286 184 2007
Fragments arranged by size:
2286 2007 1127 610 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2969 10
Size: 54 1425 343 1137 3255
Fragments arranged by size:
3255 1425 1137 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2979 3080 3335 4172 4531 4690
Size: 648 1383 430 101 255 837 359 159
Cuts at: 4690 518
Size: 2042
Fragments arranged by size:
2042 1383 837 648 430 359 255 159
101
MspA1I CmG'CkG
Cuts at: 1606 2421 2824 3630 4307 5455 5700 1606
Size: 815 403 806 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 806 677 403 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 2199 2741 3784 2199
Size: 542 1043 4629
Fragments arranged by size:
4629 1043 542
PspOMI G'GGCC_C
Cuts at: 2123 2123
Size: 6214
PstI C_TGCA'G
Cuts at: 830 3566 830
Size: 2736 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2824 2824
Size: 6214
SacI G_AGCT'C
Cuts at: 2889 2889
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 3224 4462 6174 3224
Size: 1238 1712 3264
Fragments arranged by size:
3264 1712 1238
SfcI C'TryA_G
Cuts at: 826 1045 2361 2539 3056 3562 4697 5378 5569
Size: 219 1316 178 517 506 1135 681 191
Cuts at: 5569 826
Size: 1471
Fragments arranged by size:
1471 1316 1135 681 517 506 219 191
178
SfiI GGCCn_nnn'nGGCC
Cuts at: 2329 2329
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3122 4318 5219 5481 820
Size: 197 926 486 693 1196 901 262 1553
Fragments arranged by size:
1553 1196 926 901 693 486 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 2323 1517
Size: 806 5408
StyI C'CwwG_G
Cuts at: 513 1065 2004 3653 513
Size: 552 939 1649 3074
Fragments arranged by size:
3074 1649 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3052 4241 4258 4411 4596 5041 325
Size: 1255 1472 1189 17 153 185 445 1498
Fragments arranged by size:
1498 1472 1255 1189 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 4460 6172
Size: 274 80 33 51 1443 1245 1238 1712
Cuts at: 6172 96
Size: 138
Fragments arranged by size:
1712 1443 1245 1238 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2710 2742 6174 6206 2710
Size: 32 3432 32 2718
Fragments arranged by size:
3432 2718 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3178 3178
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfoI SrfI SwaI