Restriction map for
pFN21AE5677
(Circular) (Six-base) MAPSORT of: pFN21AE5677.seq Check: 865 from: 1 to: 6442
pFN21AE5677 6442 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 3393 4569 4942 1563
Size: 1830 1176 373 3063
Fragments arranged by size:
3063 1830 1176 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2118 3542 4505 5889 6157 1632
Size: 406 80 1424 963 1384 268 1917
Fragments arranged by size:
1917 1424 1384 963 406 268 80
AfeI AGC'GCT
Cuts at: 2730 2730
Size: 6442
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2220 5341 6318 1203
Size: 1017 3121 977 1327
Fragments arranged by size:
3121 1327 1017 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2450 2856 3443 1184
Size: 1266 406 587 4183
Fragments arranged by size:
4183 1266 587 406
AlwNI CAG_nnn'CTG
Cuts at: 3646 4185 5757 3646
Size: 539 1572 4331
Fragments arranged by size:
4331 1572 539
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 3212 3751 3977 4129 6238 1493
Size: 1719 539 226 152 2109 1697
Fragments arranged by size:
2109 1719 1697 539 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2661 4996 160
Size: 872 1629 2335 1606
Fragments arranged by size:
2335 1629 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2200 3761 1391
Size: 809 1561 4072
Fragments arranged by size:
4072 1561 809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 2379 1548
Size: 831 5611
BfrBI ATG'CAT
Cuts at: 2507 2507
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2357 2492 5053
Size: 107 122 71 631 605 685 135 2561
Cuts at: 5053 136
Size: 1525
Fragments arranged by size:
2561 1525 685 631 605 135 122 107
71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 2393 3859 2393
Size: 1466 4976
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4503 5659 1790
Size: 1846 867 1156 2573
Fragments arranged by size:
2573 1846 1156 867
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2548 3421 4173
Size: 416 53 83 399 24 1131 873 752
Cuts at: 4173 5131 442
Size: 958 1753
Fragments arranged by size:
1753 1131 958 873 752 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5102 1239
Size: 3863 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5105 882
Size: 617 3606 2219
Fragments arranged by size:
3606 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2461 4233 493
Size: 711 1257 1772 2702
Fragments arranged by size:
2702 1772 1257 711
BsaBI GATnn'nnATC
Cuts at: 2084 3573 6436 2084
Size: 1489 2863 2090
Fragments arranged by size:
2863 2090 1489
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4631 5266 275
Size: 53 83 186 936 2716 382 635 1451
Fragments arranged by size:
2716 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2076 4875 5273 5547 5694 6105 2076
Size: 2799 398 274 147 411 2413
Fragments arranged by size:
2799 2413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 3240 3270 1178
Size: 30 165 30 784 30 1023 30 4350
Fragments arranged by size:
4350 1023 784 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 3018 1186
Size: 898 934 4610
Fragments arranged by size:
4610 934 898
BseYI C'CCAG_C
Cuts at: 5645 6141 5645
Size: 496 5946
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2391 2519 2918 1163
Size: 573 486 169 128 399 4687
Fragments arranged by size:
4687 573 486 399 169 128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2416 3892 4653 5681 1540
Size: 275 186 415 1476 761 1028 2301
Fragments arranged by size:
2301 1476 1028 761 415 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2200 2533 4503 4588 5659 2016
Size: 184 333 1970 85 1071 2799
Fragments arranged by size:
2799 1970 1071 333 184 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2200 2533 3636 3761 4503 4588
Size: 399 226 184 333 1103 125 742 85
Cuts at: 4588 5659 1391
Size: 1071 2174
Fragments arranged by size:
2174 1103 1071 742 399 333 226 184
125 85
BspEI T'CCGG_A
Cuts at: 2076 6105 2076
Size: 4029 2413
BspHI T'CATG_A
Cuts at: 3055 4331 6061 3055
Size: 1276 1730 3436
Fragments arranged by size:
3436 1730 1276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2573 4937 5111 65
Size: 1183 1325 2364 174 1396
Fragments arranged by size:
2364 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 5086 5273 1700
Size: 110 713 2563 187 2869
Fragments arranged by size:
2869 2563 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 2199 4502 5514 2199
Size: 2303 1012 3127
Fragments arranged by size:
3127 2303 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2073 3772 4139 4524 4541 5993 6079 6091
Size: 101 1699 367 385 17 1452 86 12
Cuts at: 6091 6437 1972
Size: 346 1977
Fragments arranged by size:
1977 1699 1452 385 367 346 101 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2587 2950 513
Size: 552 939 583 363 4005
Fragments arranged by size:
4005 939 583 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2722 487
Size: 683 56 1496 4207
Fragments arranged by size:
4207 1496 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2234 2404 2930 3647 3958 4752 4772 2016
Size: 218 170 526 717 311 794 20 3686
Fragments arranged by size:
3686 794 717 526 311 218 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2455 2484
Size: 54 1425 50 275 18 315 310 29
Cuts at: 2484 3889 4778 6407 8
Size: 1405 889 1629 43
Fragments arranged by size:
1629 1425 1405 889 315 310 275 54
50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2340 4372 1353
Size: 789 198 2032 3423
Fragments arranged by size:
3423 2032 789 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2198 2198
Size: 6442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2118 3542 4505 5102 5889 6157
Size: 393 406 80 1424 963 597 787 268
Cuts at: 6157 1239
Size: 1524
Fragments arranged by size:
1524 1424 963 787 597 406 393 268
80
EcoO109I rG'GnC_Cy
Cuts at: 3321 3321
Size: 6442
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 6442
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2732 5589 1678
Size: 1054 2857 2531
Fragments arranged by size:
2857 2531 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5083 5115 5157 5189 1373
Size: 32 135 32 3511 32 42 32 2626
Fragments arranged by size:
3511 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 2902 3786 4032 669
Size: 988 281 832 132 884 246 3079
Fragments arranged by size:
3079 988 884 832 281 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 2770 2902 4032 2770
Size: 132 1130 5180
Fragments arranged by size:
5180 1130 132
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2436 5556 5740 1305
Size: 1131 3120 184 2007
Fragments arranged by size:
3120 2007 1131 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2486 10
Size: 54 1425 343 654 3966
Fragments arranged by size:
3966 1425 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2562 4400 4759 4918 518
Size: 648 843 553 1838 359 159 2042
Fragments arranged by size:
2042 1838 843 648 553 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2297 2945 3087 3285 3341 3440 3858
Size: 452 239 648 142 198 56 99 418
Cuts at: 3858 4535 5683 5928 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 648 452 418 245 239
198 142 99 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 2587 2950 513
Size: 552 939 583 363 4005
Fragments arranged by size:
4005 939 583 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 2509 2509
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2224 2639 3124 3800 5345 1132
Size: 97 995 415 485 676 1545 2229
Fragments arranged by size:
2229 1545 995 676 485 415 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 2220 5341 2220
Size: 3121 3321
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6442
PfoI T'CCnGG_A
Cuts at: 3585 3585
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PmlI CAC'GTG
Cuts at: 2461 2461
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3321 3321
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 2945 1613
Size: 1332 5110
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 2644 3170 3247 3794 830
Size: 1814 526 77 547 3478
Fragments arranged by size:
3478 1814 547 526 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2297 2945 3341 2297
Size: 648 396 5398
Fragments arranged by size:
5398 648 396
SacI G_AGCT'C
Cuts at: 2200 2200
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2640 3166 3243 3790 4925 5606 5797
Size: 219 1595 526 77 547 1135 681 191
Cuts at: 5797 826
Size: 1471
Fragments arranged by size:
1595 1471 1135 681 547 526 219 191
77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 2639 3800 1132
Size: 1507 1161 3774
Fragments arranged by size:
3774 1507 1161
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2587 2950 3881 513
Size: 552 939 207 376 363 931 3074
Fragments arranged by size:
3074 939 931 552 376 363 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2430 4469 4486 4639 4824 5269 325
Size: 1255 850 2039 17 153 185 445 1498
Fragments arranged by size:
2039 1498 1255 850 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2272 4688 6400
Size: 274 80 33 51 1443 295 2416 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
2416 1712 1443 295 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 3240 3272 6402 6434 2207
Size: 32 1001 32 3130 32 2215
Fragments arranged by size:
3130 2215 1001 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3547 3778 3547
Size: 231 6211
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI