Restriction map for
pFN21AE5672
(Circular) (Six-base) MAPSORT of: pFN21AE5672.seq Check: 9642 from: 1 to: 6340
pFN21AE5672 6340 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3223 3223
Size: 6340
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2487 3291 3527 3683 1937
Size: 550 804 236 156 4594
Fragments arranged by size:
4594 804 550 236 156
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 3533 4403 5787 6055 1632
Size: 1400 501 870 1384 268 1917
Fragments arranged by size:
1917 1400 1384 870 501 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 5239 6216 1203
Size: 4036 977 1327
Fragments arranged by size:
4036 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 4696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 1184
Size: 1644 4696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 6308
AlwNI CAG_nnn'CTG
Cuts at: 2049 3055 4083 5655 2049
Size: 1006 1028 1572 2734
Fragments arranged by size:
2734 1572 1028 1006
ApaI G_GGCC'C
Cuts at: 2270 2270
Size: 6340
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 3649 3875 4027 6136 1493
Size: 2156 226 152 2109 1697
Fragments arranged by size:
2156 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3176 3176
Size: 6340
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2261 2869 2948 3059 3665 1870
Size: 73 318 608 79 111 606 4545
Fragments arranged by size:
4545 608 606 318 111 79 73
AvrII C'CTAG_G
Cuts at: 2371 2371
Size: 6340
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2733 3661 5116 618
Size: 325 217 627 318 628 928 1455 1842
Fragments arranged by size:
1842 1455 928 628 627 325 318 217
BanII G_rGCy'C
Cuts at: 1391 2270 3659 1391
Size: 879 1389 4072
Fragments arranged by size:
4072 1389 879
BbeI G_GCGC'C
Cuts at: 2737 2737
Size: 6340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2187 2467 928
Size: 971 282 6 280 4801
Fragments arranged by size:
4801 971 282 280 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2593 2627 4531 4565 1903
Size: 34 50 34 572 34 1904 34 3678
Fragments arranged by size:
3678 1904 572 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2783 4951 136
Size: 107 122 71 631 605 1111 2168 1525
Fragments arranged by size:
2168 1525 1111 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 2411 2633 3757 2411
Size: 222 1124 4994
Fragments arranged by size:
4994 1124 222
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2270 3315 4401 5557 1790
Size: 320 160 1045 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 1045 320 160
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 4071 5029
Size: 416 53 83 399 24 1158 1496 958
Cuts at: 5029 442
Size: 1753
Fragments arranged by size:
1753 1496 1158 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 2570 2785 2930 5000 1239
Size: 817 514 215 145 2070 2579
Fragments arranged by size:
2579 2070 817 514 215 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 2630 3152 4465 5330 5628 2564
Size: 66 522 1313 865 298 3276
Fragments arranged by size:
3276 1313 865 522 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3395 5003 882
Size: 617 1896 1608 2219
Fragments arranged by size:
2219 1896 1608 617
BsaAI yAC'GTr
Cuts at: 493 1204 2917 4131 493
Size: 711 1713 1214 2702
Fragments arranged by size:
2702 1713 1214 711
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2734 4147 4529 5164
Size: 53 83 186 936 1201 1413 382 635
Cuts at: 5164 275
Size: 1451
Fragments arranged by size:
1451 1413 1201 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3188 4773 5171 5445 5592 6003 3188
Size: 1585 398 274 147 411 3525
Fragments arranged by size:
3525 1585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 2747 2777 2912
Size: 30 165 30 783 30 531 30 135
Cuts at: 2912 2942 1178
Size: 30 4576
Fragments arranged by size:
4576 783 531 165 135 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2204 2207 2447 2567 2630 2699 2758
Size: 852 166 3 240 120 63 69 59
Cuts at: 2758 2867 1186
Size: 109 4659
Fragments arranged by size:
4659 852 240 166 120 109 69 63
59 3
BseYI C'CCAG_C
Cuts at: 2270 2557 3314 5543 6039 2270
Size: 287 757 2229 496 2571
Fragments arranged by size:
2571 2229 757 496 287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5767
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2461 2472 3790 4551 5579 1540
Size: 275 186 460 11 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 460 275 186 11
BsiHKAI G_wGCw'C
Cuts at: 2523 2817 2916 3306 4401 4486 5557 2523
Size: 294 99 390 1095 85 1071 3306
Fragments arranged by size:
3306 1095 1071 390 294 99 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
BsmBI CGTCTCn'nnnn_
Cuts at: 2020 2824 2020
Size: 804 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2270 2523 2817 2916 3306 3315
Size: 399 320 160 253 294 99 390 9
Cuts at: 3315 3659 4401 4486 5557 1391
Size: 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 344 320 294
253 160 99 85 9
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2668 2904 2964 3223 3405 3695 844
Size: 1824 236 60 259 182 290 3489
Fragments arranged by size:
3489 1824 290 259 236 182 60
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 3040 4227 1394
Size: 429 797 420 1187 3507
Fragments arranged by size:
3507 1187 797 429 420
BsrDI GCAATG_nn'
Cuts at: 65 1248 3435 4835 5009 65
Size: 1183 2187 1400 174 1396
Fragments arranged by size:
2187 1400 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2351 4984 5171 1700
Size: 110 541 2633 187 2869
Fragments arranged by size:
2869 2633 541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 2665 2667 2996 3176 1925
Size: 740 2 329 180 5089
Fragments arranged by size:
5089 740 329 180 2
BssSI C'ACGA_G
Cuts at: 2657 4400 5412 2657
Size: 1743 1012 3585
Fragments arranged by size:
3585 1743 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2662 3310 794
Size: 1868 648 3824
Fragments arranged by size:
3824 1868 648
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 5279
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 2794
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 2649 3670 4037 4422 4439 5891 5977 5989
Size: 677 1021 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1021 677 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3528 3528
Size: 6340
Bsu36I CC'TnA_GG
Cuts at: 2984 2984
Size: 6340
BtgI C'CryG_G
Cuts at: 513 1065 2134 2823 2847 3261 3357 3375 513
Size: 552 1069 689 24 414 96 18 3478
Fragments arranged by size:
3478 1069 689 552 414 96 24 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3241 487
Size: 683 56 2015 3586
Fragments arranged by size:
3586 2015 683 56
BtsI GCAGTG_nn'
Cuts at: 3856 4650 4670 3856
Size: 794 20 5526
Fragments arranged by size:
5526 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6305
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DraIII CAC_nnn'GTG
Cuts at: 3310 3310
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 5347 809
Size: 507 1155 2876 1802
Fragments arranged by size:
2876 1802 1155 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2317 2349 2382
Size: 54 1425 50 275 18 487 32 33
Cuts at: 2382 2469 2775 3027 3091 3787 4676 6305 8
Size: 87 306 252 64 696 889 1629 43
Fragments arranged by size:
1629 1425 889 696 487 306 275 252
87 64 54 50 43 33 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2469 3787 1537
Size: 275 657 1318 4090
Fragments arranged by size:
4090 1318 657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 2705 3257 4270 1353
Size: 864 488 552 1013 3423
Fragments arranged by size:
3423 1013 864 552 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 4927 5301 5447 387
Size: 1064 138 1116 2222 374 146 1280
Fragments arranged by size:
2222 1280 1116 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2570 2785 2930 3032 3533 4403
Size: 393 424 514 215 145 102 501 870
Cuts at: 4403 5000 5787 6055 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 514 501 424 393
268 215 145 102
EcoO109I rG'GnC_Cy
Cuts at: 2046 2266 2267 2046
Size: 220 1 6119
Fragments arranged by size:
6119 220 1
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 3152 4846 3152
Size: 1694 4646
FspAI rTGC'GCAy
Cuts at: 3152 3152
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2737 2834 2840 2992 3006 5487 1678
Size: 1059 97 6 152 14 2481 2531
Fragments arranged by size:
2531 2481 1059 152 97 14 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4981 5013 5055 5087 1373
Size: 32 135 32 3409 32 42 32 2626
Fragments arranged by size:
3409 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6340
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KasI G'GCGC_C
Cuts at: 2733 2733
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2319 2384 3029 10
Size: 54 1425 343 487 65 645 3321
Fragments arranged by size:
3321 1425 645 487 343 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 2810 3260 4298 4657 4816 518
Size: 648 1626 18 450 1038 359 159 2042
Fragments arranged by size:
2042 1626 1038 648 450 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2332 2482 2731 3052 3359 3756 4433 5581
Size: 726 150 249 321 307 397 677 1148
Cuts at: 5581 5826 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 726 677 397 321 307 249
245 150
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 6340
NarI GG'CG_CC
Cuts at: 2734 2734
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 3261 513
Size: 552 2196 3592
Fragments arranged by size:
3592 2196 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2713 3698 5243 1132
Size: 97 1484 985 1545 2229
Fragments arranged by size:
2229 1545 1484 985 97
PasI CC'CwG_GG
Cuts at: 1348 2101 1348
Size: 753 5587
PciI A'CATG_T
Cuts at: 5239 5239
Size: 6340
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3100 3235 1108
Size: 1992 135 4213
Fragments arranged by size:
4213 1992 135
PfoI T'CCnGG_A
Cuts at: 2033 2547 2907 2033
Size: 514 360 5466
Fragments arranged by size:
5466 514 360
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2917 2917
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 4409 4441 5922 5954 2747
Size: 32 1630 32 1481 32 3133
Fragments arranged by size:
3133 1630 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PspOMI G'GGCC_C
Cuts at: 2266 2266
Size: 6340
PspXI vC'TCGA_Gb
Cuts at: 2948 3059 2948
Size: 111 6229
PstI C_TGCA'G
Cuts at: 830 3692 830
Size: 2862 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2332 2482 3052 2332
Size: 150 570 5620
Fragments arranged by size:
5620 570 150
SacII CC_GC'GG
Cuts at: 3360 3360
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SapI GCTCTTCn'nnn_
Cuts at: 3257 3257
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SexAI A'CCwGG_T
Cuts at: 3012 3021 3012
Size: 9 6331
SfcI C'TryA_G
Cuts at: 826 1045 2041 3502 3688 4823 5504 5695 826
Size: 219 996 1461 186 1135 681 191 1471
Fragments arranged by size:
1471 1461 1135 996 681 219 191 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 2783 2783
Size: 6340
SfoI GGC'GCC
Cuts at: 2735 2735
Size: 6340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 2645 2948 3059 3167 4444
Size: 197 926 636 66 303 111 108 1277
Cuts at: 4444 5345 5607 820
Size: 901 262 1553
Fragments arranged by size:
1553 1277 926 901 636 303 262 197
111 108 66
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6340
StyI C'CwwG_G
Cuts at: 513 1065 2371 2781 3261 3779 513
Size: 552 1306 410 480 518 3074
Fragments arranged by size:
3074 1306 552 518 480 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 2303 4367 4384 4537 4722 5167
Size: 1255 513 210 2064 17 153 185 445
Cuts at: 5167 325
Size: 1498
Fragments arranged by size:
2064 1498 1255 513 445 210 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2583 4586 6298
Size: 274 80 33 51 1443 606 2003 1712
Cuts at: 6298 96
Size: 138
Fragments arranged by size:
2003 1712 1443 606 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 2912 2944 6300 6332 2186
Size: 32 694 32 3356 32 2194
Fragments arranged by size:
3356 2194 694 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 2948 3059 1943
Size: 1005 111 5224
Fragments arranged by size:
5224 1005 111
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 2762 4469 2762
Size: 1707 4633
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI BaeI BbvCI BfrBI BplI Bpu10I BsiWI EcoICRI
EcoNI EcoRV FalI FseI NruI NsiI PacI PpuMI
PsrI RsrII SacI SanDI SrfI SwaI XcmI