Restriction map for
pFN21AE5670
(Circular) (Six-base) MAPSORT of: pFN21AE5670.seq Check: 2827 from: 1 to: 6304
pFN21AE5670 6304 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3432 3432
Size: 6304
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4114 278
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 3453 3625 3453
Size: 172 6132
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 3285 4367 5751 6019 1632
Size: 1070 583 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1082 1070 583 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 5203 6180 1203
Size: 4000 977 1327
Fragments arranged by size:
4000 1327 977
AgeI A'CCGG_T
Cuts at: 5135 5135
Size: 6304
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2548 1184
Size: 1364 4940
AlwNI CAG_nnn'CTG
Cuts at: 2121 2152 2494 4047 5619 2121
Size: 31 342 1553 1572 2806
Fragments arranged by size:
2806 1572 1553 342 31
ApaI G_GGCC'C
Cuts at: 2069 2069
Size: 6304
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 2543 2711 2870 2943 3613 3839 3991 6100
Size: 1050 168 159 73 670 226 152 2109
Cuts at: 6100 1493
Size: 1697
Fragments arranged by size:
2109 1697 1050 670 226 168 159 152
73
AscI GG'CGCG_CC
Cuts at: 3414 3414
Size: 6304
AseI AT'TA_AT
Cuts at: 160 1032 2886 4858 160
Size: 872 1854 1972 1606
Fragments arranged by size:
1972 1854 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 3629 1870
Size: 73 345 1341 4545
Fragments arranged by size:
4545 1341 345 73
AvrII C'CTAG_G
Cuts at: 2798 2798
Size: 6304
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3453 3625 5080 618
Size: 325 217 627 1666 172 1455 1842
Fragments arranged by size:
1842 1666 1455 627 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2069 2412 2753 3623 1391
Size: 678 343 341 870 4072
Fragments arranged by size:
4072 870 678 343 341
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 928
Size: 971 341 4992
Fragments arranged by size:
4992 971 341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2461 2495 4495 4529 1903
Size: 34 524 34 2000 34 3678
Fragments arranged by size:
3678 2000 524 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 2331 3190 1548
Size: 783 859 4662
Fragments arranged by size:
4662 859 783
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 4915 136
Size: 107 122 71 631 605 751 2492 1525
Fragments arranged by size:
2492 1525 751 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 2126 3721 2126
Size: 1595 4709
Bme1580I G_kGCm'C
Cuts at: 1790 2069 2564 2586 4365 5521 1790
Size: 279 495 22 1779 1156 2573
Fragments arranged by size:
2573 1779 1156 495 279 22
BmgBI CAC'GTC
Cuts at: 1318 2677 3475 1318
Size: 1359 798 4147
Fragments arranged by size:
4147 1359 798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 2099 2482
Size: 416 53 83 399 24 646 36 383
Cuts at: 2482 2551 4035 4993 442
Size: 69 1484 958 1753
Fragments arranged by size:
1753 1484 958 646 416 399 383 83
69 53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2916 3252 3319 4964 1239
Size: 1677 336 67 1645 2579
Fragments arranged by size:
2579 1677 1645 336 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4429 5294 5592 4429
Size: 865 298 5141
Fragments arranged by size:
5141 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2941 4967 882
Size: 617 1442 2026 2219
Fragments arranged by size:
2219 2026 1442 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 4095 493
Size: 711 1377 1514 2702
Fragments arranged by size:
2702 1514 1377 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2193 4111 4493
Size: 53 83 186 936 496 164 1918 382
Cuts at: 4493 5128 275
Size: 635 1451
Fragments arranged by size:
1918 1451 936 635 496 382 186 164
83 53
BsaWI w'CCGG_w
Cuts at: 3546 4737 5135 5409 5556 5967 3546
Size: 1191 398 274 147 411 3883
Fragments arranged by size:
3883 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2558 2588 3219 3249 1178
Size: 30 165 30 1155 30 631 30 4233
Fragments arranged by size:
4233 1155 631 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2587 1186
Size: 1401 4903
BseYI C'CCAG_C
Cuts at: 2062 2084 5507 6003 2062
Size: 22 3423 496 2363
Fragments arranged by size:
3423 2363 496 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 5086
Fragments arranged by size:
5086 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3754 4515 5543 1540
Size: 275 186 1753 761 1028 2301
Fragments arranged by size:
2301 1753 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 2753 4365 4450 5521 2412
Size: 341 1612 85 1071 3195
Fragments arranged by size:
3195 1612 1071 341 85
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
BsmBI CGTCTCn'nnnn_
Cuts at: 2205 2205
Size: 6304
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2412 2564 2586 2753 3623 4365
Size: 399 279 343 152 22 167 870 742
Cuts at: 4365 4450 5521 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 870 742 399 343 279 167
152 85 22
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3432 3659 844
Size: 2588 227 3489
Fragments arranged by size:
3489 2588 227
BsrBI CCG'CTC
Cuts at: 1394 1823 4191 1394
Size: 429 2368 3507
Fragments arranged by size:
3507 2368 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2646 3160 4799 4973 65
Size: 1183 1398 514 1639 174 1396
Fragments arranged by size:
1639 1398 1396 1183 514 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4948 5135 1700
Size: 110 3138 187 2869
Fragments arranged by size:
3138 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2202 96
Size: 2106 4198
BssHII G'CGCG_C
Cuts at: 1925 3414 1925
Size: 1489 4815
BssSI C'ACGA_G
Cuts at: 4364 5376 4364
Size: 1012 5292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2527 2702 794
Size: 1733 175 4396
Fragments arranged by size:
4396 1733 175
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 3089 1184
Size: 1905 4399
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299
Size: 1662 367 385 17 1452 86 12 346
Cuts at: 6299 1972
Size: 1977
Fragments arranged by size:
1977 1662 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3130 3165 513
Size: 552 939 1126 35 3652
Fragments arranged by size:
3652 1126 939 552 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 2525 3820 4614 4634 2525
Size: 1295 794 20 4195
Fragments arranged by size:
4195 1295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3526 3608 3526
Size: 82 6222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3375 3492 3751
Size: 54 1425 50 275 18 1545 117 259
Cuts at: 3751 4640 6269 8
Size: 889 1629 43
Fragments arranged by size:
1629 1545 1425 889 275 259 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3751 1537
Size: 275 1939 4090
Fragments arranged by size:
4090 1939 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4234 1353
Size: 2881 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 4891 5265 5411 387
Size: 1064 138 451 2851 374 146 1280
Fragments arranged by size:
2851 1280 1064 451 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2751 2410
Size: 341 5963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2702 2916 3252 3285 3319 4367 4964
Size: 393 1070 214 336 33 34 1048 597
Cuts at: 4964 5751 6019 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1070 1048 787 597 393 336 268
214 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3344 3344
Size: 6304
EcoO109I rG'GnC_Cy
Cuts at: 2740 2740
Size: 6304
EcoRI G'AATT_C
Cuts at: 2711 3613 3991 2711
Size: 902 378 5024
Fragments arranged by size:
5024 902 378
FspI TGC'GCA
Cuts at: 4810 4810
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 5451 1678
Size: 3773 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2919 2951 4945 4977 5019
Size: 32 135 32 1347 32 1994 32 42
Cuts at: 5019 5051 1373
Size: 32 2626
Fragments arranged by size:
2626 1994 1347 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3648 3894 669
Size: 988 281 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KpnI G_GTAC'C
Cuts at: 3457 3629 3457
Size: 172 6132
MfeI C'AATT_G
Cuts at: 2967 3903 2967
Size: 936 5368
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5418 5602 1305
Size: 4113 184 2007
Fragments arranged by size:
4113 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2530 3170 4262 4621 4780 518
Size: 648 843 521 640 1092 359 159 2042
Fragments arranged by size:
2042 1092 843 648 640 521 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2629 3367 3720 4397 5545 5790 1606
Size: 546 477 738 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 546 477 353 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3130 3165 513
Size: 552 939 1126 35 3652
Fragments arranged by size:
3652 1126 939 552 35
NdeI CA'TA_TG
Cuts at: 387 3149 387
Size: 2762 3542
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 3751 3751
Size: 6304
NspI r_CATG'y
Cuts at: 1132 1229 2134 2525 2644 3662 5207 1132
Size: 97 905 391 119 1018 1545 2229
Fragments arranged by size:
2229 1545 1018 905 391 119 97
PasI CC'CwG_GG
Cuts at: 1348 2020 1348
Size: 672 5632
PciI A'CATG_T
Cuts at: 5203 5203
Size: 6304
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2111 3469 1108
Size: 1003 1358 3943
Fragments arranged by size:
3943 1358 1003
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 6304
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2740 2740
Size: 6304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PspOMI G'GGCC_C
Cuts at: 2065 2065
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2157 2325 2628 3656 830
Size: 1327 168 303 1028 3478
Fragments arranged by size:
3478 1327 1028 303 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2152 2152
Size: 6304
SacI G_AGCT'C
Cuts at: 2412 2753 2412
Size: 341 5963
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 4552 6264 4552
Size: 1712 4592
SexAI A'CCwGG_T
Cuts at: 3449 3449
Size: 6304
SfcI C'TryA_G
Cuts at: 826 1045 2153 2321 2624 3652 4787 5468 5659
Size: 219 1108 168 303 1028 1135 681 191
Cuts at: 5659 826
Size: 1471
Fragments arranged by size:
1471 1135 1108 1028 681 303 219 191
168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 3631 1872
Size: 1759 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4408 5309 5571 820
Size: 197 926 2465 901 262 1553
Fragments arranged by size:
2465 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 2134 2525 3662 1132
Size: 1002 391 1137 3774
Fragments arranged by size:
3774 1137 1002 391
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 2590 1517
Size: 1073 5231
StyI C'CwwG_G
Cuts at: 513 1065 2004 2798 3130 3165 3468 3743 513
Size: 552 939 794 332 35 303 275 3074
Fragments arranged by size:
3074 939 794 552 332 303 275 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3131 3376 4331 4348 4501 4686 5131
Size: 1255 1551 245 955 17 153 185 445
Cuts at: 5131 325
Size: 1498
Fragments arranged by size:
1551 1498 1255 955 445 245 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 4550 6262
Size: 274 80 33 51 1443 225 2348 1712
Cuts at: 6262 96
Size: 138
Fragments arranged by size:
2348 1712 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 6264 6296 3219
Size: 32 3013 32 3227
Fragments arranged by size:
3227 3013 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6304
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 3629 1870
Size: 1759 4545
XmnI GAAnn'nnTTC
Cuts at: 2715 4433 2715
Size: 1718 4586
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4112 276
Size: 53 83 186 936 2578 2468
Fragments arranged by size:
2578 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI