Restriction map for
pFN21AE5668
(Circular) (Six-base) MAPSORT of: pFN21AE5668.seq Check: 9909 from: 1 to: 6289
pFN21AE5668 6289 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2879 2879
Size: 6289
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2723 3477 4099 278
Size: 53 83 186 936 1187 754 622 2468
Fragments arranged by size:
2468 1187 936 754 622 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2147 2568 2645 4352 5736 6004 1632
Size: 401 114 421 77 1707 1384 268 1917
Fragments arranged by size:
1917 1707 1384 421 401 268 114 77
AfeI AGC'GCT
Cuts at: 2545 2545
Size: 6289
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 2589 5188 6165 1203
Size: 1386 2599 977 1327
Fragments arranged by size:
2599 1386 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 2719 5018 2719
Size: 2299 3990
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2759 1184
Size: 1575 4714
AlwNI CAG_nnn'CTG
Cuts at: 3047 4032 5604 3047
Size: 985 1572 3732
Fragments arranged by size:
3732 1572 985
ApaI G_GGCC'C
Cuts at: 2146 2146
Size: 6289
ApaLI G'TGCA_C
Cuts at: 2185 2987 4346 5502 2185
Size: 802 1359 1156 2972
Fragments arranged by size:
2972 1359 1156 802
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2599 3561 3614 1870
Size: 73 656 962 53 4545
Fragments arranged by size:
4545 962 656 73 53
BamHI G'GATC_C
Cuts at: 3619 3986 3619
Size: 367 5922
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2867 3610 5065 618
Size: 325 217 627 1080 743 1455 1842
Fragments arranged by size:
1842 1455 1080 743 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2146 2162 3297 3363 3608 1391
Size: 755 16 1135 66 245 4072
Fragments arranged by size:
4072 1135 755 245 66 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 2557 2633 928
Size: 971 657 1 76 4584
Fragments arranged by size:
4584 971 657 76 1
BbvCI CC'TCA_GC
Cuts at: 3160 3461 3160
Size: 301 5988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2228 2262 2480 2514 3396 3430 4480
Size: 34 291 34 218 34 882 34 1050
Cuts at: 4480 4514 1903
Size: 34 3678
Fragments arranged by size:
3678 1050 882 291 218 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3507 4900 136
Size: 107 122 71 631 605 1835 1393 1525
Fragments arranged by size:
1835 1525 1393 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 3706 3706
Size: 6289
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2146 2189 2493 2870 2991 4350 5506
Size: 347 9 43 304 377 121 1359 1156
Cuts at: 5506 1790
Size: 2573
Fragments arranged by size:
2573 1359 1156 377 347 304 121 43
9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4020 4978 442
Size: 416 53 83 399 24 2603 958 1753
Fragments arranged by size:
2603 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 3003 3377 4949 1239
Size: 1190 574 374 1572 2579
Fragments arranged by size:
2579 1572 1190 574 374
Bpu10I CC'TnA_GC
Cuts at: 3160 3461 3160
Size: 301 5988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4414 5279 5577 4414
Size: 865 298 5126
Fragments arranged by size:
5126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3251 4952 882
Size: 617 1752 1701 2219
Fragments arranged by size:
2219 1752 1701 617
BsaAI yAC'GTr
Cuts at: 493 1204 4080 493
Size: 711 2876 2702
Fragments arranged by size:
2876 2702 711
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2720 3474 4096
Size: 53 83 186 936 507 680 754 622
Cuts at: 4096 4478 5113 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 754 680 635 622 507 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4722 5120 5394 5541 5952 4722
Size: 398 274 147 411 5059
Fragments arranged by size:
5059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 1178
Size: 30 165 30 687 30 5347
Fragments arranged by size:
5347 687 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2039 2042 1186
Size: 850 3 3 5433
Fragments arranged by size:
5433 850 3 3
BseYI C'CCAG_C
Cuts at: 2336 2486 2785 3149 5492 5988 2336
Size: 150 299 364 2343 496 2637
Fragments arranged by size:
2637 2343 496 364 299 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2219 2765 3060 1163
Size: 573 333 150 546 295 4392
Fragments arranged by size:
4392 573 546 333 295 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2482 2618 3739 4500 5528 1540
Size: 275 186 481 136 1121 761 1028 2301
Fragments arranged by size:
2301 1121 1028 761 481 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2189 2991 3363 4350 4435 5506 2189
Size: 802 372 987 85 1071 2972
Fragments arranged by size:
2972 1071 987 802 372 85
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 2942 2711
Size: 231 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2146 2162 2189 2493 2870 2991
Size: 399 347 9 16 27 304 377 121
Cuts at: 2991 3297 3363 3608 4350 4435 5506 1391
Size: 306 66 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 377 347 306 304
245 121 85 66 27 16 9
BspEI T'CCGG_A
Cuts at: 5952 5952
Size: 6289
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2700 2859 2879 3593 3644 844
Size: 1856 159 20 714 51 3489
Fragments arranged by size:
3489 1856 714 159 51 20
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 4176 1394
Size: 429 293 2060 3507
Fragments arranged by size:
3507 2060 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2656 4784 4958 65
Size: 1183 1408 2128 174 1396
Fragments arranged by size:
2128 1408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 4933 5120 1700
Size: 110 237 2886 187 2869
Fragments arranged by size:
2886 2869 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 4349 5361 4349
Size: 1012 5277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3047 794
Size: 2253 4036
BstBI TT'CG_AA
Cuts at: 2727 5093 2727
Size: 2366 3923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6289
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3172
Size: 6289
BstYI r'GATC_y
Cuts at: 1972 2748 3043 3144 3619 3986 4371 4388 5840
Size: 776 295 101 475 367 385 17 1452
Cuts at: 5840 5926 5938 6284 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 776 475 385 367 346 295
101 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2618 2886 3135 513
Size: 552 939 614 268 249 3667
Fragments arranged by size:
3667 939 614 552 268 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5550
Fragments arranged by size:
5550 683 56
BtsI GCAGTG_nn'
Cuts at: 2081 2925 3445 3805 4599 4619 2081
Size: 844 520 360 794 20 3751
Fragments arranged by size:
3751 844 794 520 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DraIII CAC_nnn'GTG
Cuts at: 2280 2280
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5296 809
Size: 507 3980 1802
Fragments arranged by size:
3980 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2615 3174 3736
Size: 54 1425 50 275 18 785 559 562
Cuts at: 3736 4625 6254 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 785 562 559 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2615 3736 1537
Size: 275 803 1121 4090
Fragments arranged by size:
4090 1121 803 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4219 1353
Size: 2866 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4876 5250 5396 387
Size: 1064 138 3287 374 146 1280
Fragments arranged by size:
3287 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3361 3361
Size: 6289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2147 2429 2568 2645 3003 3377
Size: 393 401 114 282 139 77 358 374
Cuts at: 3377 4352 4949 5736 6004 1239
Size: 975 597 787 268 1524
Fragments arranged by size:
1524 975 787 597 401 393 374 358
282 268 139 114 77
EcoO109I rG'GnC_Cy
Cuts at: 2143 2143
Size: 6289
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6289
FspI TGC'GCA
Cuts at: 4795 4795
Size: 6289
HaeII r_GCGC'y
Cuts at: 1678 2547 5436 1678
Size: 869 2889 2531
Fragments arranged by size:
2889 2531 869
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 2705 2737 2740
Size: 32 135 32 533 32 568 32 3
Cuts at: 2740 2772 4930 4962 5004 5036 1373
Size: 32 2158 32 42 32 2626
Fragments arranged by size:
2626 2158 568 533 135 42 32 32
32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5403 5587 1305
Size: 4098 184 2007
Fragments arranged by size:
4098 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3176 10
Size: 54 1425 343 1344 3123
Fragments arranged by size:
3123 1425 1344 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2741 3221 4247 4606 4765 518
Size: 648 1221 354 480 1026 359 159 2042
Fragments arranged by size:
2042 1221 1026 648 480 359 354 159
MspA1I CmG'CkG
Cuts at: 1606 2407 2620 2785 2839 3705 4382 5530 5775
Size: 801 213 165 54 866 677 1148 245
Cuts at: 5775 1606
Size: 2120
Fragments arranged by size:
2120 1148 866 801 677 245 213 165
54
NcoI C'CATG_G
Cuts at: 513 1065 2004 2886 3135 513
Size: 552 939 882 249 3667
Fragments arranged by size:
3667 939 882 552 249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 2615 3736 2615
Size: 1121 5168
NspI r_CATG'y
Cuts at: 1132 1229 2467 2509 2995 3647 5192 1132
Size: 97 1238 42 486 652 1545 2229
Fragments arranged by size:
2229 1545 1238 652 486 97 42
PasI CC'CwG_GG
Cuts at: 1348 2862 1348
Size: 1514 4775
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6289
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5871 5903 4358
Size: 32 1481 32 4744
Fragments arranged by size:
4744 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6289
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2142 2142
Size: 6289
PstI C_TGCA'G
Cuts at: 830 2421 2543 3641 830
Size: 1591 122 1098 3478
Fragments arranged by size:
3478 1591 1098 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 2407 2785 2407
Size: 378 5911
SacI G_AGCT'C
Cuts at: 3363 3363
Size: 6289
SacII CC_GC'GG
Cuts at: 2621 2621
Size: 6289
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 2204 3083 4537 6249 2204
Size: 879 1454 1712 2244
Fragments arranged by size:
2244 1712 1454 879
SexAI A'CCwGG_T
Cuts at: 2958 2958
Size: 6289
SfcI C'TryA_G
Cuts at: 826 1045 2417 2539 2917 3637 4772 5453 5644
Size: 219 1372 122 378 720 1135 681 191
Cuts at: 5644 826
Size: 1471
Fragments arranged by size:
1471 1372 1135 720 681 378 219 191
122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3616 1872
Size: 1744 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4393 5294 5556 820
Size: 197 926 2450 901 262 1553
Fragments arranged by size:
2450 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4080 493
Size: 3587 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 2063 3278 1517
Size: 546 1215 4528
Fragments arranged by size:
4528 1215 546
StyI C'CwwG_G
Cuts at: 513 1065 2004 2886 3135 3728 513
Size: 552 939 882 249 593 3074
Fragments arranged by size:
3074 939 882 593 552 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2496 3290 4316 4333 4486 4671
Size: 1255 444 472 794 1026 17 153 185
Cuts at: 4671 5116 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1026 794 472 445 444 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3014 3027
Size: 274 80 33 51 1443 225 812 13
Cuts at: 3027 3081 4535 6247 96
Size: 54 1454 1712 138
Fragments arranged by size:
1712 1454 1443 812 274 225 138 80
54 51 33 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 6249 6281 2090
Size: 32 4127 32 2098
Fragments arranged by size:
4127 2098 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3473 1316
Size: 2157 4132
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2789 2789
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 3614 1870
Size: 1744 4545
XmnI GAAnn'nnTTC
Cuts at: 4418 4418
Size: 6289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2721 3475 4097 276
Size: 53 83 186 936 1187 754 622 2468
Fragments arranged by size:
2468 1187 936 754 622 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI
SwaI