Restriction map for
pFN21AE5666
(Circular) (Six-base) MAPSORT of: pFN21AE5666.seq Check: 4458 from: 1 to: 6265
pFN21AE5666 6265 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 3024 4328 5712 5980 1632
Size: 652 740 1304 1384 268 1917
Fragments arranged by size:
1917 1384 1304 740 652 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2982 5164 6141 1203
Size: 1779 2182 977 1327
Fragments arranged by size:
2182 1779 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 2867 1166
Size: 1701 4564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 3410 4008 5580 3410
Size: 598 1572 4095
Fragments arranged by size:
4095 1572 598
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2630 3574 3800 3952 6061 1493
Size: 1137 944 226 152 2109 1697
Fragments arranged by size:
2109 1697 1137 944 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 3345 3590 1870
Size: 73 166 1236 245 4545
Fragments arranged by size:
4545 1236 245 166 73
BamHI G'GATC_C
Cuts at: 2177 3219 3595 3962 2177
Size: 1042 376 367 4480
Fragments arranged by size:
4480 1042 376 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3278 3586 5041 618
Size: 325 217 627 1491 308 1455 1842
Fragments arranged by size:
1842 1491 1455 627 325 308 217
BanII G_rGCy'C
Cuts at: 1391 3584 1391
Size: 2193 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 2859 1548
Size: 1311 4954
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 4326 5482 1790
Size: 2536 1156 2573
Fragments arranged by size:
2573 2536 1156
BmgBI CAC'GTC
Cuts at: 1318 2023 3065 1318
Size: 705 1042 4518
Fragments arranged by size:
4518 1042 705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 2941 2955 3158 3319 4925 1239
Size: 1478 224 14 203 161 1606 2579
Fragments arranged by size:
2579 1606 1478 224 203 161 14
Bpu10I CC'TnA_GC
Cuts at: 3482 3482
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 4390 5255 5553 2230
Size: 2160 865 298 2942
Fragments arranged by size:
2942 2160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 2525 2548 2676 2741 4928 882
Size: 617 732 294 23 128 65 2187 2219
Fragments arranged by size:
2219 2187 732 617 294 128 65 23
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2673 2703 1178
Size: 30 165 30 1270 30 4740
Fragments arranged by size:
4740 1270 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3188 1186
Size: 2002 4263
BseYI C'CCAG_C
Cuts at: 5468 5964 5468
Size: 496 5769
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3408 1163
Size: 573 1672 4020
Fragments arranged by size:
4020 1672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4326 4411 5482 4326
Size: 85 1071 5109
Fragments arranged by size:
5109 1071 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3584 4326 4411 5482 1391
Size: 399 1794 742 85 1071 2174
Fragments arranged by size:
2174 1794 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2662 3303 4152 1394
Size: 429 839 641 849 3507
Fragments arranged by size:
3507 849 839 641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 2865 4325 5337 2865
Size: 1460 1012 3793
Fragments arranged by size:
3793 1460 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2016 794
Size: 1222 5043
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2322 3058 3118 3448 2322
Size: 736 60 330 5139
Fragments arranged by size:
5139 736 330 60
BstYI r'GATC_y
Cuts at: 1972 2177 2642 3219 3351 3595 3962 4347 4364
Size: 205 465 577 132 244 367 385 17
Cuts at: 4364 5816 5902 5914 6260 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 577 465 385 367 346 244
205 132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2405 3363 513
Size: 552 1340 958 3415
Fragments arranged by size:
3415 1340 958 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 2582 3781 4575 4595 2523
Size: 59 1199 794 20 4193
Fragments arranged by size:
4193 1199 794 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2488 2523 529
Size: 35 1924 35 4271
Fragments arranged by size:
4271 1924 35 35
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230
Size: 54 1425 50 275 18 1882 889 1629
Cuts at: 6230 8
Size: 43
Fragments arranged by size:
1882 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2421 4195 1353
Size: 1068 1774 3423
Fragments arranged by size:
3423 1774 1068
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2655 4852 5226 5372 387
Size: 1064 138 1066 2197 374 146 1280
Fragments arranged by size:
2197 1280 1066 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2284 2717 2941 2955 3024 3158 3319
Size: 393 652 433 224 14 69 134 161
Cuts at: 3319 4328 4925 5712 5980 1239
Size: 1009 597 787 268 1524
Fragments arranged by size:
1524 1009 787 652 597 433 393 268
224 161 134 69 14
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 6265
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 5412 1678
Size: 3734 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2154 2721 2753 2848
Size: 32 135 32 550 32 567 32 95
Cuts at: 2848 2880 3211 3243 4906 4938 4980 5012 1373
Size: 32 331 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 567 550 331 135 95 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2167 2967 5379 5563 1305
Size: 862 800 2412 184 2007
Fragments arranged by size:
2412 2007 862 800 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4443
Fragments arranged by size:
4443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2320 2444 2867 4223 4582 4741 518
Size: 648 1154 124 423 1356 359 159 2042
Fragments arranged by size:
2042 1356 1154 648 423 359 159 124
MspA1I CmG'CkG
Cuts at: 1606 3681 4358 5506 5751 1606
Size: 2075 677 1148 245 2120
Fragments arranged by size:
2120 2075 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2405 3363 513
Size: 552 1340 958 3415
Fragments arranged by size:
3415 1340 958 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3362 3623 5168 1132
Size: 97 2133 261 1545 2229
Fragments arranged by size:
2229 2133 1545 261 97
PasI CC'CwG_GG
Cuts at: 1348 3215 1348
Size: 1867 4398
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 3297 1108
Size: 2148 41 4076
Fragments arranged by size:
4076 2148 41
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 2907 3835 2907
Size: 928 5337
PstI C_TGCA'G
Cuts at: 830 2530 3617 830
Size: 1700 1087 3478
Fragments arranged by size:
3478 1700 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 3182 4513 6225 3182
Size: 1331 1712 3222
Fragments arranged by size:
3222 1712 1331
SfcI C'TryA_G
Cuts at: 826 1045 2526 3321 3613 4748 5429 5620 826
Size: 219 1481 795 292 1135 681 191 1471
Fragments arranged by size:
1481 1471 1135 795 681 292 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 2111 3592 1872
Size: 239 1481 4545
Fragments arranged by size:
4545 1481 239
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 4369 5270 5532 820
Size: 197 926 266 2160 901 262 1553
Fragments arranged by size:
2160 1553 926 901 266 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3362 3623 1132
Size: 2230 261 3774
Fragments arranged by size:
3774 2230 261
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 2704 3477 1517
Size: 1187 773 4305
Fragments arranged by size:
4305 1187 773
StyI C'CwwG_G
Cuts at: 513 1065 2170 2220 2405 2677 3111 3363 3704
Size: 552 1105 50 185 272 434 252 341
Cuts at: 3704 513
Size: 3074
Fragments arranged by size:
3074 1105 552 434 341 272 252 185
50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2700 4292 4309 4462 4647 5092 325
Size: 1255 1120 1592 17 153 185 445 1498
Fragments arranged by size:
1592 1498 1255 1120 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3180 4511 6223
Size: 274 80 33 51 1443 1203 1331 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1712 1443 1331 1203 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2673 2705 6225 6257 2076
Size: 32 565 32 3520 32 2084
Fragments arranged by size:
3520 2084 565 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 1316
Size: 708 5557
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3445 3445
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 2109 3590 1870
Size: 239 1481 4545
Fragments arranged by size:
4545 1481 239
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI