Restriction map for
pFN21AE5664
(Circular) (Six-base) MAPSORT of: pFN21AE5664.seq Check: 6181 from: 1 to: 6277
pFN21AE5664 6277 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3620 1937
Size: 1683 4594
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4340 5724 5992 1632
Size: 2708 1384 268 1917
Fragments arranged by size:
2708 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 3112 1166
Size: 1946 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2049 3130 1184
Size: 865 1081 4331
Fragments arranged by size:
4331 1081 865
AlwNI CAG_nnn'CTG
Cuts at: 2161 4020 5592 2161
Size: 1859 1572 2846
Fragments arranged by size:
2846 1859 1572
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2271 2407 2744 3586 3812 3964 6073 1493
Size: 778 136 337 842 226 152 2109 1697
Fragments arranged by size:
2109 1697 842 778 337 226 152 136
AseI AT'TA_AT
Cuts at: 160 1032 2889 4831 160
Size: 872 1857 1942 1606
Fragments arranged by size:
1942 1857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 3602 1870
Size: 73 1150 509 4545
Fragments arranged by size:
4545 1150 509 73
AvrII C'CTAG_G
Cuts at: 3052 3052
Size: 6277
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3596 1391
Size: 2205 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3483 928
Size: 971 1584 3722
Fragments arranged by size:
3722 1584 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3117 3502 4169 5379 3117
Size: 385 667 1210 4015
Fragments arranged by size:
4015 1210 667 385
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3285 4888 136
Size: 107 122 71 631 605 1613 1603 1525
Fragments arranged by size:
1613 1603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 4338 5494 1790
Size: 2548 1156 2573
Fragments arranged by size:
2573 2548 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 4008 4966
Size: 416 53 83 399 24 754 1837 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
1837 1753 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 4937 1239
Size: 859 2839 2579
Fragments arranged by size:
2839 2579 859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 4402 5267 5565 2236
Size: 2166 865 298 2948
Fragments arranged by size:
2948 2166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 2958 6271 2958
Size: 3313 2964
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2440 4710 5108 5382 5529 5940 2440
Size: 2270 398 274 147 411 2777
Fragments arranged by size:
2777 2270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2071 2101 3134 3164 1178
Size: 30 165 30 668 30 1033 30 4291
Fragments arranged by size:
4291 1033 668 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6277
BseYI C'CCAG_C
Cuts at: 5480 5976 5480
Size: 496 5781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3246 1163
Size: 573 1510 4194
Fragments arranged by size:
4194 1510 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4338 4423 5494 4338
Size: 85 1071 5121
Fragments arranged by size:
5121 1071 85
BsmI GAATG_Cn'
Cuts at: 2825 3788 3881 2825
Size: 963 93 5221
Fragments arranged by size:
5221 963 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3596 4338 4423 5494 1391
Size: 399 1806 742 85 1071 2174
Fragments arranged by size:
2174 1806 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3632 844
Size: 2788 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 1394
Size: 429 2341 3507
Fragments arranged by size:
3507 2341 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2841 3607 3974 4359 4376 5828 5914 5926
Size: 869 766 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 869 766 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3366 3366
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 2423 513
Size: 552 1358 4367
Fragments arranged by size:
4367 1358 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 3793 4587 4607 3580
Size: 213 794 20 5250
Fragments arranged by size:
5250 794 213 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 2749 2940 3581 2749
Size: 191 641 5445
Fragments arranged by size:
5445 641 191
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 3724 4613
Size: 54 1425 50 275 18 1456 438 889
Cuts at: 4613 6242 8
Size: 1629 43
Fragments arranged by size:
1629 1456 1425 889 438 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3519 4207 1353
Size: 2166 688 3423
Fragments arranged by size:
3423 2166 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 4340 4937 5724 5992 1239
Size: 393 466 2242 597 787 268 1524
Fragments arranged by size:
2242 1524 787 597 466 393 268
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2403 2819 2403
Size: 416 5861
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4918 4950 4992 5024 1373
Size: 32 135 32 3346 32 42 32 2626
Fragments arranged by size:
3346 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3621 3867 669
Size: 988 281 196 1487 246 3079
Fragments arranged by size:
3079 1487 988 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 2134 3867 2134
Size: 1733 4544
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 2854 3876 2854
Size: 1022 5255
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 5391 5575 1305
Size: 1719 2367 184 2007
Fragments arranged by size:
2367 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3288 10
Size: 54 1425 343 1456 2999
Fragments arranged by size:
2999 1456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3112 4235 4594 4753 518
Size: 648 1946 1123 359 159 2042
Fragments arranged by size:
2042 1946 1123 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2326 3693 4370 5518 5763 1606
Size: 720 1367 677 1148 245 2120
Fragments arranged by size:
2120 1367 1148 720 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5725
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2999 3014 3635 5180 1132
Size: 97 1770 15 621 1545 2229
Fragments arranged by size:
2229 1770 1545 621 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PstI C_TGCA'G
Cuts at: 830 3019 3629 830
Size: 2189 610 3478
Fragments arranged by size:
3478 2189 610
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2326 2326
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 2221 3003 3347 4525 6237 2221
Size: 782 344 1178 1712 2261
Fragments arranged by size:
2261 1712 1178 782 344
SfcI C'TryA_G
Cuts at: 826 1045 3015 3625 4760 5441 5632 826
Size: 219 1970 610 1135 681 191 1471
Fragments arranged by size:
1970 1471 1135 681 610 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 3093 4381 5282 5544 820
Size: 197 926 308 842 1288 901 262 1553
Fragments arranged by size:
1553 1288 926 901 842 308 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3014 3635 1132
Size: 1882 621 3774
Fragments arranged by size:
3774 1882 621
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 2247 1517
Size: 730 5547
StyI C'CwwG_G
Cuts at: 513 1065 2593 3052 3716 513
Size: 552 1528 459 664 3074
Fragments arranged by size:
3074 1528 664 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4304 4321 4474 4659 5104 325
Size: 1255 2724 17 153 185 445 1498
Fragments arranged by size:
2724 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2125 2219 2294
Size: 274 80 33 51 1443 148 94 75
Cuts at: 2294 2473 3001 3345 4523 6235 96
Size: 179 528 344 1178 1712 138
Fragments arranged by size:
1712 1443 1178 528 344 274 179 148
138 94 80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6237 6269 6237
Size: 32 6245
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 3093 1943
Size: 1150 5127
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 2588 4406 2588
Size: 1818 4459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI