Restriction map for
pFN21AE5662
(Circular) (Six-base) MAPSORT of: pFN21AE5662.seq Check: 2396 from: 1 to: 6289
pFN21AE5662 6289 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2366 2698 2366
Size: 332 5957
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4099 278
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3632 1937
Size: 1695 4594
Acc65I G'GTAC_C
Cuts at: 3610 3610
Size: 6289
AclI AA'CG_TT
Cuts at: 1563 4416 4789 1563
Size: 2853 373 3063
Fragments arranged by size:
3063 2853 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2930 3176 3528 4352 5736 6004 1632
Size: 872 426 246 352 824 1384 268 1917
Fragments arranged by size:
1917 1384 872 824 426 352 268 246
AfeI AGC'GCT
Cuts at: 2744 2744
Size: 6289
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6092
AflIII A'CryG_T
Cuts at: 1203 2915 5188 6165 1203
Size: 1712 2273 977 1327
Fragments arranged by size:
2273 1712 1327 977
AgeI A'CCGG_T
Cuts at: 5120 5120
Size: 6289
AhdI GACnn_n'nnGTC
Cuts at: 3504 5018 3504
Size: 1514 4775
AleI CACnn'nnGTG
Cuts at: 1166 3137 1166
Size: 1971 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2252
Size: 32 6257
AlwNI CAG_nnn'CTG
Cuts at: 3022 3376 3514 4032 5604 3022
Size: 354 138 518 1572 3707
Fragments arranged by size:
3707 1572 518 354 138
ApaI G_GGCC'C
Cuts at: 2327 2343 3190 3315 2327
Size: 16 847 125 5301
Fragments arranged by size:
5301 847 125 16
ApaLI G'TGCA_C
Cuts at: 4346 5502 4346
Size: 1156 5133
ApoI r'AATT_y
Cuts at: 1493 3598 3824 3976 6085 1493
Size: 2105 226 152 2109 1697
Fragments arranged by size:
2109 2105 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4843 160
Size: 872 3811 1606
Fragments arranged by size:
3811 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 3062 3614 1870
Size: 73 957 162 552 4545
Fragments arranged by size:
4545 957 552 162 73
BamHI G'GATC_C
Cuts at: 3619 3986 3619
Size: 367 5922
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 2878 3610 5065 618
Size: 325 217 627 839 252 732 1455 1842
Fragments arranged by size:
1842 1455 839 732 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2327 2343 2994 3112 3190 3315 3588 3608
Size: 936 16 651 118 78 125 273 20
Cuts at: 3608 1391
Size: 4072
Fragments arranged by size:
4072 936 651 273 125 118 78 20
16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2309 2462 2918 928
Size: 971 410 153 456 4299
Fragments arranged by size:
4299 971 456 410 153
BbvCI CC'TCA_GC
Cuts at: 2715 2715
Size: 6289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2852 2886 4480 4514 1903
Size: 34 50 34 831 34 1594 34 3678
Fragments arranged by size:
3678 1594 831 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4181 5391 4181
Size: 1210 5079
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6289
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3009 4900 136
Size: 107 122 71 631 605 1337 1891 1525
Fragments arranged by size:
1891 1525 1337 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6284 6284
Size: 6289
BlpI GC'TnA_GC
Cuts at: 3706 3706
Size: 6289
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2327 2343 2883 2899 3190 3315 4350
Size: 234 303 16 540 16 291 125 1035
Cuts at: 4350 5506 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1035 540 303 291 234 125
16 16
BmgBI CAC'GTC
Cuts at: 1318 3501 1318
Size: 2183 4106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4020 4978
Size: 416 53 83 399 24 2114 489 958
Cuts at: 4978 442
Size: 1753
Fragments arranged by size:
2114 1753 958 489 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2162 2279 2411 2753 3182 3462 3556
Size: 818 105 117 132 342 429 280 94
Cuts at: 3556 4949 1239
Size: 1393 2579
Fragments arranged by size:
2579 1393 818 429 342 280 132 117
105 94
Bpu10I CC'TnA_GC
Cuts at: 2498 2715 2498
Size: 217 6072
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4414 5279 5577 4414
Size: 865 298 5126
Fragments arranged by size:
5126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4952 882
Size: 617 3453 2219
Fragments arranged by size:
3453 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2657 2918 4080 493
Size: 711 1453 261 1162 2702
Fragments arranged by size:
2702 1453 1162 711 261
BsaBI GATnn'nnATC
Cuts at: 6283 6283
Size: 6289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4096 4478 5113 275
Size: 53 83 186 936 2563 382 635 1451
Fragments arranged by size:
2563 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2298 3208 4722 5120 5394 5541 5952 2298
Size: 910 1514 398 274 147 411 2635
Fragments arranged by size:
2635 1514 910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 2252 2282 1178
Size: 30 165 30 627 30 192 30 5185
Fragments arranged by size:
5185 627 192 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2212 1186
Size: 844 182 5263
Fragments arranged by size:
5263 844 182
BseYI C'CCAG_C
Cuts at: 2326 2398 3105 3168 3429 3490 5492 5988 2326
Size: 72 707 63 261 61 2002 496 2627
Fragments arranged by size:
2627 2002 707 496 261 72 63 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2726 3203 1163
Size: 573 339 651 477 4249
Fragments arranged by size:
4249 651 573 477 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3739 4500 5528 1540
Size: 275 186 1738 761 1028 2301
Fragments arranged by size:
2301 1738 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 2595 2833 4350 4435 5506 2294
Size: 301 238 1517 85 1071 3077
Fragments arranged by size:
3077 1517 1071 301 238 85
BsmI GAATG_Cn'
Cuts at: 3800 3893 3800
Size: 93 6196
BsmBI CGTCTCn'nnnn_
Cuts at: 2079 3491 2079
Size: 1412 4877
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2294 2327 2343 2595 2833 2883
Size: 399 234 270 33 16 252 238 50
Cuts at: 2883 2899 2994 3112 3190 3315 3588 3608 4350
Size: 16 95 118 78 125 273 20 742
Cuts at: 4350 4435 5506 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 273 270 252 238
234 125 118 95 85 78 50 33
20 16 16
BspEI T'CCGG_A
Cuts at: 2298 5952 2298
Size: 3654 2635
BspHI T'CATG_A
Cuts at: 4178 5908 4178
Size: 1730 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2366 2388 2698 3644 844
Size: 1522 22 310 946 3489
Fragments arranged by size:
3489 1522 946 310 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3049 4176 1394
Size: 429 1226 1127 3507
Fragments arranged by size:
3507 1226 1127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4784 4958 65
Size: 1183 3536 174 1396
Fragments arranged by size:
3536 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2649 3344 4933 5120 1700
Size: 110 839 695 1589 187 2869
Fragments arranged by size:
2869 1589 839 695 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6289
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6289
BssSI C'ACGA_G
Cuts at: 4349 5361 4349
Size: 1012 5277
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6289
BstBI TT'CG_AA
Cuts at: 5093 5093
Size: 6289
BstEII G'GTnAC_C
Cuts at: 1184 3331 1184
Size: 2147 4142
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 3139 2611
Size: 528 5761
BstYI r'GATC_y
Cuts at: 1972 3018 3619 3986 4371 4388 5840 5926 5938
Size: 1046 601 367 385 17 1452 86 12
Cuts at: 5938 6284 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1046 601 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2449 2449
Size: 6289
BtgI C'CryG_G
Cuts at: 513 1065 2514 3339 513
Size: 552 1449 825 3463
Fragments arranged by size:
3463 1449 825 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2855 487
Size: 683 56 1629 3921
Fragments arranged by size:
3921 1629 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 3805 4599 4619 3424
Size: 381 794 20 5094
Fragments arranged by size:
5094 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6254
DraI TTT'AAA
Cuts at: 3593 3593
Size: 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 2855 5296 809
Size: 507 780 759 2441 1802
Fragments arranged by size:
2441 1802 780 759 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2983 3283
Size: 54 1425 50 275 18 337 816 300
Cuts at: 3283 3342 3736 4625 6254 8
Size: 59 394 889 1629 43
Fragments arranged by size:
1629 1425 889 816 394 337 300 275
59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3736 1537
Size: 275 1924 4090
Fragments arranged by size:
4090 1924 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 4219 1353
Size: 836 2030 3423
Fragments arranged by size:
3423 2030 836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 4876 5250 5396 387
Size: 1064 138 975 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 975 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2162 2279 2411 2504 2753 2930
Size: 393 425 105 117 132 93 249 177
Cuts at: 2930 3176 3182 3462 3528 3556 4352 4949 5736
Size: 246 6 280 66 28 796 597 787
Cuts at: 5736 6004 1239
Size: 268 1524
Fragments arranged by size:
1524 796 787 597 425 393 280 268
249 246 177 132 117 105 93 66
28 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3161 3161
Size: 6289
EcoO109I rG'GnC_Cy
Cuts at: 2137 2323 2339 2340 2377 3187 3311 3312 2137
Size: 186 16 1 37 810 124 1 5114
Fragments arranged by size:
5114 810 186 124 37 16 1 1
EcoRI G'AATT_C
Cuts at: 3598 3976 3598
Size: 378 5911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 6289
FseI GG_CCGG'CC
Cuts at: 3348 3348
Size: 6289
FspI TGC'GCA
Cuts at: 2426 4795 2426
Size: 2369 3920
HaeII r_GCGC'y
Cuts at: 1678 2746 5436 1678
Size: 1068 2690 2531
Fragments arranged by size:
2690 2531 1068
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2432 2464 3490
Size: 32 135 32 458 32 370 32 1026
Cuts at: 3490 3522 4930 4962 5004 5036 1373
Size: 32 1408 32 42 32 2626
Fragments arranged by size:
2626 1408 1026 458 370 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3633 3879 669
Size: 988 281 1695 246 3079
Fragments arranged by size:
3079 1695 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6289
HpaI GTT'AAC
Cuts at: 3879 3879
Size: 6289
KpnI G_GTAC'C
Cuts at: 3614 3614
Size: 6289
MfeI C'AATT_G
Cuts at: 3888 3888
Size: 6289
MluI A'CGCG_T
Cuts at: 6165 6165
Size: 6289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 5403 5587 1305
Size: 1320 2778 184 2007
Fragments arranged by size:
2778 2007 1320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 3285 10
Size: 54 1425 343 337 1116 3014
Fragments arranged by size:
3014 1425 1116 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2945 3137 3179 3227 4247 4606
Size: 648 1443 336 192 42 48 1020 359
Cuts at: 4606 4765 518
Size: 159 2042
Fragments arranged by size:
2042 1443 1020 648 359 336 192 159
48 42
MspA1I CmG'CkG
Cuts at: 1606 2059 2173 3494 3705 4382 5530 5775 1606
Size: 453 114 1321 211 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 453 245 211 114
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 6289
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 4288
Fragments arranged by size:
4288 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6289
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 6289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6289
NotI GC'GGCC_GC
Cuts at: 3736 3736
Size: 6289
NspI r_CATG'y
Cuts at: 1132 1229 3184 3647 5192 1132
Size: 97 1955 463 1545 2229
Fragments arranged by size:
2229 1955 1545 463 97
PasI CC'CwG_GG
Cuts at: 1348 2318 1348
Size: 970 5319
PciI A'CATG_T
Cuts at: 5188 5188
Size: 6289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6289
PfoI T'CCnGG_A
Cuts at: 2388 2529 3438 3478 3572 2388
Size: 141 909 40 94 5105
Fragments arranged by size:
5105 909 141 94 40
PmeI GTTT'AAAC
Cuts at: 3593 3593
Size: 6289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2252 2284 4358 4390 5871 5903 2252
Size: 32 2074 32 1481 32 2638
Fragments arranged by size:
2638 2074 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2377 2137
Size: 240 6049
PshAI GACnn'nnGTC
Cuts at: 1613 3456 1613
Size: 1843 4446
PsiI TTA'TAA
Cuts at: 3859 3859
Size: 6289
PspOMI G'GGCC_C
Cuts at: 2323 2339 3186 3311 2323
Size: 16 847 125 5301
Fragments arranged by size:
5301 847 125 16
PstI C_TGCA'G
Cuts at: 830 2349 3381 3641 830
Size: 1519 1032 260 3478
Fragments arranged by size:
3478 1519 1032 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6289
PvuII CAG'CTG
Cuts at: 2059 3494 2059
Size: 1435 4854
SalI G'TCGA_C
Cuts at: 1936 3631 1936
Size: 1695 4594
SbfI CC_TGCA'GG
Cuts at: 3641 3641
Size: 6289
ScaI AGT'ACT
Cuts at: 2069 4537 6249 2069
Size: 2468 1712 2109
Fragments arranged by size:
2468 2109 1712
SexAI A'CCwGG_T
Cuts at: 2796 2796
Size: 6289
SfcI C'TryA_G
Cuts at: 826 1045 2345 3377 3637 4772 5453 5644 826
Size: 219 1300 1032 260 1135 681 191 1471
Fragments arranged by size:
1471 1300 1135 1032 681 260 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6289
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6289
SmaI CCC'GGG
Cuts at: 1872 3616 1872
Size: 1744 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4393 5294 5556 820
Size: 197 926 2450 901 262 1553
Fragments arranged by size:
2450 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2657 4080 493
Size: 2164 1423 2702
Fragments arranged by size:
2702 2164 1423
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6289
SphI G_CATG'C
Cuts at: 1132 3647 1132
Size: 2515 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5
Size: 47 4161 2081
Fragments arranged by size:
4161 2081 47
StuI AGG'CCT
Cuts at: 1517 2207 3536 1517
Size: 690 1329 4270
Fragments arranged by size:
4270 1329 690
StyI C'CwwG_G
Cuts at: 513 1065 2514 3728 513
Size: 552 1449 1214 3074
Fragments arranged by size:
3074 1449 1214 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 4316 4333 4486 4671 5116 325
Size: 1255 810 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 810 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 4535 6247
Size: 274 80 33 51 1443 90 2468 1712
Cuts at: 6247 96
Size: 138
Fragments arranged by size:
2468 1712 1443 274 138 90 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3289 3321 3551 3583 6249 6281 3289
Size: 32 230 32 2666 32 3297
Fragments arranged by size:
3297 2666 230 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2798 1316
Size: 1482 4807
XbaI T'CTAG_A
Cuts at: 3625 3625
Size: 6289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3401 3401
Size: 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6289
XmaI C'CCGG_G
Cuts at: 1870 3614 1870
Size: 1744 4545
XmnI GAAnn'nnTTC
Cuts at: 3386 4418 3386
Size: 1032 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4097 276
Size: 53 83 186 936 2563 2468
Fragments arranged by size:
2563 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII
EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI