Restriction map for
pFN21AE5661
(Circular) (Six-base) MAPSORT of: pFN21AE5661.seq Check: 7065 from: 1 to: 6250
pFN21AE5661 6250 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2460 2460
Size: 6250
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2582 2856 4060 278
Size: 53 83 186 936 1046 274 1204 2468
Fragments arranged by size:
2468 1204 1046 936 274 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3593 1937
Size: 1656 4594
Acc65I G'GTAC_C
Cuts at: 3571 3571
Size: 6250
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2927 4313 5697 5965 1632
Size: 499 796 1386 1384 268 1917
Fragments arranged by size:
1917 1386 1384 796 499 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 5149 6126 1203
Size: 3946 977 1327
Fragments arranged by size:
3946 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 2578 4979 2578
Size: 2401 3849
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 2468 1184
Size: 1089 195 4966
Fragments arranged by size:
4966 1089 195
AlwNI CAG_nnn'CTG
Cuts at: 2386 2428 2548 3993 5565 2386
Size: 42 120 1445 1572 3071
Fragments arranged by size:
3071 1572 1445 120 42
ApaI G_GGCC'C
Cuts at: 3393 3393
Size: 6250
ApaLI G'TGCA_C
Cuts at: 3098 4307 5463 3098
Size: 1209 1156 3885
Fragments arranged by size:
3885 1209 1156
ApoI r'AATT_y
Cuts at: 1493 2563 3082 3131 3559 3785 3937 6046 1493
Size: 1070 519 49 428 226 152 2109 1697
Fragments arranged by size:
2109 1697 1070 519 428 226 152 49
AseI AT'TA_AT
Cuts at: 160 1032 4804 160
Size: 872 3772 1606
Fragments arranged by size:
3772 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 2309 3032 3575 1870
Size: 73 366 723 543 4545
Fragments arranged by size:
4545 723 543 366 73
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2501 3410 3571 5026 618
Size: 325 217 627 714 909 161 1455 1842
Fragments arranged by size:
1842 1455 909 714 627 325 217 161
BanII G_rGCy'C
Cuts at: 1391 2310 3393 3441 3569 1391
Size: 919 1083 48 128 4072
Fragments arranged by size:
4072 1083 919 128 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5279
BbvCI CC'TCA_GC
Cuts at: 2183 2183
Size: 6250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4142 5352 3245
Size: 897 1210 4143
Fragments arranged by size:
4143 1210 897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6250
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 2213 2286
Size: 107 122 71 631 605 450 91 73
Cuts at: 2286 3535 4861 136
Size: 1249 1326 1525
Fragments arranged by size:
1525 1326 1249 631 605 450 122 107
91 73 71
BglII A'GATC_T
Cuts at: 6245 6245
Size: 6250
BlpI GC'TnA_GC
Cuts at: 3515 3667 3515
Size: 152 6098
Bme1580I G_kGCm'C
Cuts at: 1790 3102 3393 4311 5467 1790
Size: 1312 291 918 1156 2573
Fragments arranged by size:
2573 1312 1156 918 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6250
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 3396 3981
Size: 416 53 83 399 24 957 1022 585
Cuts at: 3981 4939 442
Size: 958 1753
Fragments arranged by size:
1753 1022 958 957 585 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2117 2260 3235 4910 1239
Size: 857 21 143 975 1675 2579
Fragments arranged by size:
2579 1675 975 857 143 21
Bpu10I CC'TnA_GC
Cuts at: 2183 2183
Size: 6250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3527 4375 5240 5538 3527
Size: 848 865 298 4239
Fragments arranged by size:
4239 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4913 882
Size: 617 1098 2316 2219
Fragments arranged by size:
2316 2219 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 2212 4041 493
Size: 711 1008 1829 2702
Fragments arranged by size:
2702 1829 1008 711
BsaBI GATnn'nnATC
Cuts at: 2366 6244 2366
Size: 3878 2372
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2579 2853 4057 4439
Size: 53 83 186 936 1046 274 1204 382
Cuts at: 4439 5074 275
Size: 635 1451
Fragments arranged by size:
1451 1204 1046 936 635 382 274 186
83 53
BsaWI w'CCGG_w
Cuts at: 4683 5081 5355 5502 5913 4683
Size: 398 274 147 411 5020
Fragments arranged by size:
5020 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 2873 2903 3029
Size: 30 165 30 1377 30 63 30 126
Cuts at: 3029 3059 1178
Size: 30 4369
Fragments arranged by size:
4369 1377 165 126 63 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2801 3050 1186
Size: 1615 249 4386
Fragments arranged by size:
4386 1615 249
BseYI C'CCAG_C
Cuts at: 2640 3010 3403 5453 5949 2640
Size: 370 393 2050 496 2941
Fragments arranged by size:
2941 2050 496 393 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2963 1163
Size: 573 1227 4450
Fragments arranged by size:
4450 1227 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3474 3700 4461 5489 1540
Size: 275 186 1473 226 761 1028 2301
Fragments arranged by size:
2301 1473 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2393 2718 3102 3521 4311 4396 5467 2393
Size: 325 384 419 790 85 1071 3176
Fragments arranged by size:
3176 1071 790 419 384 325 85
BsmI GAATG_Cn'
Cuts at: 3174 3761 3854 3174
Size: 587 93 5570
Fragments arranged by size:
5570 587 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3086 3086
Size: 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2393 2718 3102 3393 3441 3521
Size: 399 520 83 325 384 291 48 80
Cuts at: 3521 3569 4311 4396 5467 1391
Size: 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 384 325 291
85 83 80 48 48
BspEI T'CCGG_A
Cuts at: 5913 5913
Size: 6250
BspHI T'CATG_A
Cuts at: 2685 4139 5869 2685
Size: 1454 1730 3066
Fragments arranged by size:
3066 1730 1454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 2460 2985 3605 844
Size: 1565 51 525 620 3489
Fragments arranged by size:
3489 1565 620 525 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2526 3448 4137 1394
Size: 429 703 922 689 3507
Fragments arranged by size:
3507 922 703 689 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3246 4745 4919 65
Size: 1183 1998 1499 174 1396
Fragments arranged by size:
1998 1499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2504 2592 4894 5081 1700
Size: 110 694 88 2302 187 2869
Fragments arranged by size:
2869 2302 694 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 3045 96
Size: 2949 3301
BssHII G'CGCG_C
Cuts at: 1925 3455 1925
Size: 1530 4720
BssSI C'ACGA_G
Cuts at: 2044 2294 4310 5322 2044
Size: 250 2016 1012 2972
Fragments arranged by size:
2972 2016 1012 250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 2408 1184
Size: 1224 5026
BstXI CCAn_nnnn'nTGG
Cuts at: 2869 3260 3421 2869
Size: 391 161 5698
Fragments arranged by size:
5698 391 161
BstYI r'GATC_y
Cuts at: 1972 2551 3159 3580 3947 4332 4349 5801 5887
Size: 579 608 421 367 385 17 1452 86
Cuts at: 5887 5899 6245 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 608 579 421 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2119 2119
Size: 6250
BtgI C'CryG_G
Cuts at: 513 1065 2004 2608 3432 513
Size: 552 939 604 824 3331
Fragments arranged by size:
3331 939 824 604 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3495 487
Size: 683 56 2269 3242
Fragments arranged by size:
3242 2269 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3766 4560 4580 2111
Size: 1655 794 20 3781
Fragments arranged by size:
3781 1655 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6215
DraI TTT'AAA
Cuts at: 3554 3554
Size: 6250
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2287 3316
Size: 54 1425 50 275 18 384 73 1029
Cuts at: 3316 3471 3697 4586 6215 8
Size: 155 226 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 384 275 226 155
73 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3471 3697 1537
Size: 275 1659 226 4090
Fragments arranged by size:
4090 1659 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 3302 4180 1353
Size: 1550 399 878 3423
Fragments arranged by size:
3423 1550 878 399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2203 2615 2948 4837 5211 5357
Size: 1064 138 614 412 333 1889 374 146
Cuts at: 5357 387
Size: 1280
Fragments arranged by size:
1889 1280 1064 614 412 374 333 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2117 2131 2260 2927 3235 4313
Size: 393 464 21 14 129 667 308 1078
Cuts at: 4313 4910 5697 5965 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1078 787 667 597 464 393 308
268 129 21 14
EcoO109I rG'GnC_Cy
Cuts at: 2220 3040 3353 3390 2220
Size: 820 313 37 5080
Fragments arranged by size:
5080 820 313 37
EcoRI G'AATT_C
Cuts at: 3559 3937 3559
Size: 378 5872
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 2428 5397 1678
Size: 750 2969 2531
Fragments arranged by size:
2969 2531 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 2785 2817 4891
Size: 32 135 32 992 32 189 32 2074
Cuts at: 4891 4923 4965 4997 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2074 992 189 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 3840 669
Size: 988 281 1656 246 3079
Fragments arranged by size:
3079 1656 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2081 748
Size: 1333 4917
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KpnI G_GTAC'C
Cuts at: 3575 3575
Size: 6250
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2859 2988 5364 5548 1305
Size: 1554 129 2376 184 2007
Fragments arranged by size:
2376 2007 1554 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 4044
Fragments arranged by size:
4044 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2867 4208 4567 4726 518
Size: 648 1701 1341 359 159 2042
Fragments arranged by size:
2042 1701 1341 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2202 2492 2610 3403 3666 4343 5491
Size: 468 128 290 118 793 263 677 1148
Cuts at: 5491 5736 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 793 677 468 290 263 245
128 118
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 6250
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4759
Fragments arranged by size:
4759 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 2962 3608 5153 1132
Size: 97 1733 646 1545 2229
Fragments arranged by size:
2229 1733 1545 646 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6250
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5348
PfoI T'CCnGG_A
Cuts at: 2568 2568
Size: 6250
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PmlI CAC'GTG
Cuts at: 2212 2212
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2807 2839 4319 4351 5832 5864 2807
Size: 32 1480 32 1481 32 3193
Fragments arranged by size:
3193 1481 1480 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 3353 2220
Size: 1133 5117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PspOMI G'GGCC_C
Cuts at: 3389 3389
Size: 6250
PstI C_TGCA'G
Cuts at: 830 3602 830
Size: 2772 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 2492 2492
Size: 6250
SacII CC_GC'GG
Cuts at: 2611 2611
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SanDI GG'GwC_CC
Cuts at: 3353 3353
Size: 6250
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 2588 4498 6210 2588
Size: 1910 1712 2628
Fragments arranged by size:
2628 1910 1712
SexAI A'CCwGG_T
Cuts at: 2742 2742
Size: 6250
SfcI C'TryA_G
Cuts at: 826 1045 2224 3598 4733 5414 5605 826
Size: 219 1179 1374 1135 681 191 1471
Fragments arranged by size:
1471 1374 1179 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 3577 1872
Size: 1705 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3542 4354 5255 5517 820
Size: 197 926 1599 812 901 262 1553
Fragments arranged by size:
1599 1553 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SspI AAT'ATT
Cuts at: 5 52 4174 5
Size: 47 4122 2081
Fragments arranged by size:
4122 2081 47
StuI AGG'CCT
Cuts at: 1517 2094 1517
Size: 577 5673
StyI C'CwwG_G
Cuts at: 513 1065 2004 3689 513
Size: 552 939 1685 3074
Fragments arranged by size:
3074 1685 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3536 4277 4294 4447 4632 5077 325
Size: 1255 1956 741 17 153 185 445 1498
Fragments arranged by size:
1956 1498 1255 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 3045 4496
Size: 274 80 33 51 1443 609 459 1451
Cuts at: 4496 6208 96
Size: 1712 138
Fragments arranged by size:
1712 1451 1443 609 459 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 6210 6242 2873
Size: 32 3305 32 2881
Fragments arranged by size:
3305 2881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3586 3586
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 3575 1870
Size: 1705 4545
XmnI GAAnn'nnTTC
Cuts at: 2822 4379 2822
Size: 1557 4693
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2580 2854 4058 276
Size: 53 83 186 936 1046 274 1204 2468
Fragments arranged by size:
2468 1204 1046 936 274 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacI SapI SfiI SfoI SrfI SwaI XcmI