Restriction map for
pFN21AE5659
(Circular) (Six-base) MAPSORT of: pFN21AE5659.seq Check: 2049 from: 1 to: 6184
pFN21AE5659 6184 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2068 2068
Size: 6184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2745 4247 5631 5899 1632
Size: 1113 1502 1384 268 1917
Fragments arranged by size:
1917 1502 1384 1113 268
AfeI AGC'GCT
Cuts at: 2192 2271 2849 2192
Size: 79 578 5527
Fragments arranged by size:
5527 578 79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 3092 1166
Size: 1926 4258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 2091 3927 5499 2091
Size: 1836 1572 2776
Fragments arranged by size:
2776 1836 1572
ApaLI G'TGCA_C
Cuts at: 2081 4241 5397 2081
Size: 2160 1156 2868
Fragments arranged by size:
2868 2160 1156
ApoI r'AATT_y
Cuts at: 1493 3209 3493 3719 3871 5980 1493
Size: 1716 284 226 152 2109 1697
Fragments arranged by size:
2109 1716 1697 284 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2296 2790 3123 3509 1870
Size: 73 353 494 333 386 4545
Fragments arranged by size:
4545 494 386 353 333 73
BamHI G'GATC_C
Cuts at: 2442 3514 3881 2442
Size: 1072 367 4745
Fragments arranged by size:
4745 1072 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3505 4960 618
Size: 325 217 627 1718 1455 1842
Fragments arranged by size:
1842 1718 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2225 2940 3503 1391
Size: 834 715 563 4072
Fragments arranged by size:
4072 834 715 563
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 928
Size: 971 590 4623
Fragments arranged by size:
4623 971 590
BbvCI CC'TCA_GC
Cuts at: 3416 3416
Size: 6184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 3455 4076 5286 3137
Size: 318 621 1210 4035
Fragments arranged by size:
4035 1210 621 318
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 3601 3601
Size: 6184
Bme1580I G_kGCm'C
Cuts at: 1790 2085 3099 3164 4245 5401 1790
Size: 295 1014 65 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 1014 295 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2327 3164 2327
Size: 837 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2633 3339 4844 1239
Size: 1394 706 1505 2579
Fragments arranged by size:
2579 1505 1394 706
Bpu10I CC'TnA_GC
Cuts at: 3416 3416
Size: 6184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4309 5174 5472 4309
Size: 865 298 5021
Fragments arranged by size:
5021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2903 4847 882
Size: 617 1404 1944 2219
Fragments arranged by size:
2219 1944 1404 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 3975 493
Size: 711 1014 1757 2702
Fragments arranged by size:
2702 1757 1014 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2403 4617 5015 5289 5436 5847 2403
Size: 2214 398 274 147 411 2740
Fragments arranged by size:
2740 2214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 1178
Size: 30 165 30 607 30 5322
Fragments arranged by size:
5322 607 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6184
BseYI C'CCAG_C
Cuts at: 2187 2669 5387 5883 2187
Size: 482 2718 496 2488
Fragments arranged by size:
2718 2488 496 482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2862 1163
Size: 573 1126 4485
Fragments arranged by size:
4485 1126 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2763 4245 4330 5401 2085
Size: 678 1482 85 1071 2868
Fragments arranged by size:
2868 1482 1071 678 85
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2225 2763 2940 3099 3164 3503
Size: 399 295 140 538 177 159 65 339
Cuts at: 3503 4245 4330 5401 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 339 295 177
159 140 85 65
BspEI T'CCGG_A
Cuts at: 2403 5847 2403
Size: 3444 2740
BspHI T'CATG_A
Cuts at: 2949 4073 5803 2949
Size: 1124 1730 3330
Fragments arranged by size:
3330 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 2371 3114 3539 844
Size: 1224 303 743 425 3489
Fragments arranged by size:
3489 1224 743 425 303
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 4071 1394
Size: 429 1657 591 3507
Fragments arranged by size:
3507 1657 591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2437 4679 4853 65
Size: 1183 1189 2242 174 1396
Fragments arranged by size:
2242 1396 1189 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 4828 5015 1700
Size: 110 390 2628 187 2869
Fragments arranged by size:
2869 2628 390 187 110
BsrGI T'GTAC_A
Cuts at: 96 2853 96
Size: 2757 3427
BssHII G'CGCG_C
Cuts at: 1925 2526 1925
Size: 601 5583
BssSI C'ACGA_G
Cuts at: 2813 3092 4244 5256 2813
Size: 279 1152 1012 3741
Fragments arranged by size:
3741 1152 1012 279
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3043 794
Size: 2249 3935
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 2442 3514 3881 4266 4283 5735 5821 5833
Size: 470 1072 367 385 17 1452 86 12
Cuts at: 5833 6179 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1072 470 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2025 513
Size: 552 960 4672
Fragments arranged by size:
4672 960 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 3700 4494 4514 3700
Size: 794 20 5370
Fragments arranged by size:
5370 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2883 3631 4520
Size: 54 1425 50 275 18 1053 748 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1053 889 748 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2561 2964 4114 1353
Size: 791 417 403 1150 3423
Fragments arranged by size:
3423 1150 791 417 403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2633 2745 3339 4247 4844 5631 5899
Size: 393 1001 112 594 908 597 787 268
Cuts at: 5899 1239
Size: 1524
Fragments arranged by size:
1524 1001 908 787 597 594 393 268
112
EcoO109I rG'GnC_Cy
Cuts at: 2108 2794 2108
Size: 686 5498
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
EcoRV GAT'ATC
Cuts at: 3409 3409
Size: 6184
FspI TGC'GCA
Cuts at: 2328 4690 2328
Size: 2362 3822
FspAI rTGC'GCAy
Cuts at: 2328 2328
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 2194 2273 2851 5331 1678
Size: 516 79 578 2480 2531
Fragments arranged by size:
2531 2480 578 516 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 2726 2758 2922
Size: 32 135 32 707 32 415 32 164
Cuts at: 2922 2954 3135 3167 4825 4857 4899 4931 1373
Size: 32 181 32 1658 32 42 32 2626
Fragments arranged by size:
2626 1658 707 415 181 164 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3397 3528 3774 669
Size: 988 281 1459 131 246 3079
Fragments arranged by size:
3079 1459 988 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 3126 5298 5482 1305
Size: 822 999 2172 184 2007
Fragments arranged by size:
2172 2007 999 822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4362
Fragments arranged by size:
4362 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3092 4142 4501 4660 518
Size: 648 1071 855 1050 359 159 2042
Fragments arranged by size:
2042 1071 1050 855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2091 3079 3600 4277 5425 5670 1606
Size: 485 988 521 677 1148 245 2120
Fragments arranged by size:
2120 1148 988 677 521 485 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2794
Size: 6184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3536 830
Size: 2706 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2091 2091
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 3532 4667 5348 5539 826
Size: 219 2487 1135 681 191 1471
Fragments arranged by size:
2487 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2792 3511 1872
Size: 920 719 4545
Fragments arranged by size:
4545 920 719
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4288 5189 5451 820
Size: 197 926 2345 901 262 1553
Fragments arranged by size:
2345 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 2161 4108 5
Size: 47 2109 1947 2081
Fragments arranged by size:
2109 2081 1947 47
StuI AGG'CCT
Cuts at: 1517 3327 1517
Size: 1810 4374
StyI C'CwwG_G
Cuts at: 513 1065 2488 3156 3623 513
Size: 552 1423 668 467 3074
Fragments arranged by size:
3074 1423 668 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2853 3270 4430
Size: 274 80 33 51 1443 876 417 1160
Cuts at: 4430 6142 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1160 876 417 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2010 2042 3294 3326 6144 6176 2010
Size: 32 1252 32 2818 32 2018
Fragments arranged by size:
2818 2018 1252 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2790 3509 1870
Size: 920 719 4545
Fragments arranged by size:
4545 920 719
XmnI GAAnn'nnTTC
Cuts at: 2138 4313 2138
Size: 2175 4009
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI