Restriction map for
pFN21AE5653
(Circular) (Six-base) MAPSORT of: pFN21AE5653.seq Check: 8027 from: 1 to: 6223
pFN21AE5653 6223 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 2236 3544 2236
Size: 1308 4915
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2822 4286 5670 5938 1632
Size: 1190 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 1190 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2320 2550 3487 1184
Size: 1136 230 937 3920
Fragments arranged by size:
3920 1136 937 230
AlwNI CAG_nnn'CTG
Cuts at: 3101 3966 5538 3101
Size: 865 1572 3786
Fragments arranged by size:
3786 1572 865
ApaLI G'TGCA_C
Cuts at: 2078 2376 4280 5436 2078
Size: 298 1904 1156 2865
Fragments arranged by size:
2865 1904 1156 298
ApoI r'AATT_y
Cuts at: 1493 2033 2063 2810 3532 3758 3910 6019 1493
Size: 540 30 747 722 226 152 2109 1697
Fragments arranged by size:
2109 1697 747 722 540 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2562 3548 1870
Size: 73 619 986 4545
Fragments arranged by size:
4545 986 619 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2726 2759 2726
Size: 33 6190
BamHI G'GATC_C
Cuts at: 3553 3920 3553
Size: 367 5856
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3544 4999 618
Size: 325 217 627 449 1308 1455 1842
Fragments arranged by size:
1842 1455 1308 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2233 2563 3408 3542 1391
Size: 842 330 845 134 4072
Fragments arranged by size:
4072 845 842 330 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2787 928
Size: 971 888 4364
Fragments arranged by size:
4364 971 888
BbvCI CC'TCA_GC
Cuts at: 2523 3213 3219 2523
Size: 690 6 5527
Fragments arranged by size:
5527 690 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4414 4448 1903
Size: 34 50 34 2393 34 3678
Fragments arranged by size:
3678 2393 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4834 136
Size: 107 122 71 631 605 3162 1525
Fragments arranged by size:
3162 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6218 6218
Size: 6223
BlpI GC'TnA_GC
Cuts at: 3640 3640
Size: 6223
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2380 3392 4284 5440 1790
Size: 292 298 1012 892 1156 2573
Fragments arranged by size:
2573 1156 1012 892 298 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3954 4912 442
Size: 416 53 83 399 24 2537 958 1753
Fragments arranged by size:
2537 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2364 3384 1056
Size: 1308 1020 3895
Fragments arranged by size:
3895 1308 1020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2958 4883 1239
Size: 962 757 1925 2579
Fragments arranged by size:
2579 1925 962 757
Bpu10I CC'TnA_GC
Cuts at: 2523 3213 3219 2523
Size: 690 6 5527
Fragments arranged by size:
5527 690 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2632 4348 5213 5511 2632
Size: 1716 865 298 3344
Fragments arranged by size:
3344 1716 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3200 4886 882
Size: 617 1701 1686 2219
Fragments arranged by size:
2219 1701 1686 617
BsaAI yAC'GTr
Cuts at: 493 1204 4014 493
Size: 711 2810 2702
Fragments arranged by size:
2810 2702 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4030 4412 5047 275
Size: 53 83 186 936 2497 382 635 1451
Fragments arranged by size:
2497 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4656 5054 5328 5475 5886 4656
Size: 398 274 147 411 4993
Fragments arranged by size:
4993 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 1178
Size: 30 165 30 817 30 5151
Fragments arranged by size:
5151 817 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6223
BseYI C'CCAG_C
Cuts at: 3034 5426 5922 3034
Size: 2392 496 3335
Fragments arranged by size:
3335 2392 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2696 3471 1163
Size: 573 297 663 775 3915
Fragments arranged by size:
3915 775 663 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3673 4434 5462 1540
Size: 275 186 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2380 3408 4284 4369 5440 2082
Size: 298 1028 876 85 1071 2865
Fragments arranged by size:
2865 1071 1028 876 298 85
BsmI GAATG_Cn'
Cuts at: 3734 3827 3734
Size: 93 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2233 2380 2563 3392 3408 3542
Size: 399 292 151 147 183 829 16 134
Cuts at: 3542 4284 4369 5440 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 829 742 399 292 183 151
147 134 85 16
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 4112 5842 4112
Size: 1730 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2132 3578 844
Size: 1288 1446 3489
Fragments arranged by size:
3489 1446 1288
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3141 4718 4892 65
Size: 1183 1893 1577 174 1396
Fragments arranged by size:
1893 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4867 5054 1700
Size: 110 3057 187 2869
Fragments arranged by size:
3057 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3296 3394 96
Size: 3200 98 2925
Fragments arranged by size:
3200 2925 98
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 3282 4283 5295 3282
Size: 1001 1012 4210
Fragments arranged by size:
4210 1012 1001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2124 794
Size: 1330 4893
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 2764 3029 1184
Size: 1580 265 4378
Fragments arranged by size:
4378 1580 265
BstXI CCAn_nnnn'nTGG
Cuts at: 2225 2225
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 3553 3920 4305 4322 5774 5860 5872 6218
Size: 1581 367 385 17 1452 86 12 346
Cuts at: 6218 1972
Size: 1977
Fragments arranged by size:
1977 1581 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2700 2700
Size: 6223
BtgI C'CryG_G
Cuts at: 513 1065 2868 3340 513
Size: 552 1803 472 3396
Fragments arranged by size:
3396 1803 552 472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 3459 3739 4533 4553 3459
Size: 280 794 20 5129
Fragments arranged by size:
5129 794 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DraIII CAC_nnn'GTG
Cuts at: 2342 2342
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3670 4559 6188
Size: 54 1425 50 275 18 1840 889 1629
Cuts at: 6188 8
Size: 43
Fragments arranged by size:
1840 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2592 2791 3312 3484 3510 4153 1353
Size: 1239 199 521 172 26 643 3423
Fragments arranged by size:
3423 1239 643 521 199 172 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4810 5184 5330 387
Size: 1064 138 3221 374 146 1280
Fragments arranged by size:
3221 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3406 3406
Size: 6223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2822 2958 4286 4883 5670 5938
Size: 393 569 621 136 1328 597 787 268
Cuts at: 5938 1239
Size: 1524
Fragments arranged by size:
1524 1328 787 621 597 569 393 268
136
EcoRI G'AATT_C
Cuts at: 3532 3910 3532
Size: 378 5845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 6223
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 5370 1678
Size: 3692 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2768 2800 4864
Size: 32 135 32 662 32 502 32 2064
Cuts at: 4864 4896 4938 4970 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2064 662 502 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2203 2426 3567 3813 669
Size: 988 281 265 223 1141 246 3079
Fragments arranged by size:
3079 1141 988 281 265 246 223
HindIII A'AGCT_T
Cuts at: 748 2148 748
Size: 1400 4823
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KpnI G_GTAC'C
Cuts at: 2240 3548 2240
Size: 1308 4915
MfeI C'AATT_G
Cuts at: 3009 3822 3009
Size: 813 5410
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2206 5337 5521 1305
Size: 901 3131 184 2007
Fragments arranged by size:
3131 2007 901 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4401
Fragments arranged by size:
4401 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4181 4540 4699 518
Size: 648 843 2172 359 159 2042
Fragments arranged by size:
2172 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2552 2800 3124 3639 4316 5464 5709 1606
Size: 946 248 324 515 677 1148 245 2120
Fragments arranged by size:
2120 1148 946 677 515 324 248 245
NcoI C'CATG_G
Cuts at: 513 1065 2868 513
Size: 552 1803 3868
Fragments arranged by size:
3868 1803 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NheI G'CTAG_C
Cuts at: 1052 2360 3380 1052
Size: 1308 1020 3895
Fragments arranged by size:
3895 1308 1020
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2947 3250 3474 3581 5126 1132
Size: 97 1718 303 224 107 1545 2229
Fragments arranged by size:
2229 1718 1545 303 224 107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6223
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2342 2544 1108
Size: 1234 202 4787
Fragments arranged by size:
4787 1234 202
PfoI T'CCnGG_A
Cuts at: 2997 2997
Size: 6223
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PspXI vC'TCGA_Gb
Cuts at: 2562 2562
Size: 6223
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2490 2522 3280 3312 2490
Size: 32 758 32 5401
Fragments arranged by size:
5401 758 32 32
PstI C_TGCA'G
Cuts at: 830 3575 830
Size: 2745 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
PvuII CAG'CTG
Cuts at: 2800 3124 2800
Size: 324 5899
SacI G_AGCT'C
Cuts at: 3408 3408
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SapI GCTCTTCn'nnn_
Cuts at: 3312 3312
Size: 6223
SbfI CC_TGCA'GG
Cuts at: 3575 3575
Size: 6223
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 2966 3571 4706 5387 5578 826
Size: 219 1921 605 1135 681 191 1471
Fragments arranged by size:
1921 1471 1135 681 605 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 2647 4327 5228 5490 820
Size: 197 926 619 85 1680 901 262 1553
Fragments arranged by size:
1680 1553 926 901 619 262 197 85
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2603 3058 1517
Size: 1086 455 4682
Fragments arranged by size:
4682 1086 455
StyI C'CwwG_G
Cuts at: 513 1065 2476 2868 3662 513
Size: 552 1411 392 794 3074
Fragments arranged by size:
3074 1411 794 552 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4250 4267 4420 4605 5050 325
Size: 1255 2670 17 153 185 445 1498
Fragments arranged by size:
2670 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2191 3296 3394
Size: 274 80 33 51 1443 214 1105 98
Cuts at: 3394 4469 6181 96
Size: 1075 1712 138
Fragments arranged by size:
1712 1443 1105 1075 274 214 138 98
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6183 6215 6183
Size: 32 6191
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6223
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 2562 1943
Size: 619 5604
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI