Restriction map for
pFN21AE5650
(Circular) (Six-base) MAPSORT of: pFN21AE5650.seq Check: 7264 from: 1 to: 6199
pFN21AE5650 6199 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2531 3542 1937
Size: 328 266 1011 4594
Fragments arranged by size:
4594 1011 328 266
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 3048 4326 4699 1563
Size: 1485 1278 373 3063
Fragments arranged by size:
3063 1485 1278 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 4262 5646 5914 1632
Size: 1076 1554 1384 268 1917
Fragments arranged by size:
1917 1554 1384 1076 268
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 6199
AflII C'TTAA_G
Cuts at: 820 1017 3083 820
Size: 197 2066 3936
Fragments arranged by size:
3936 2066 197
AflIII A'CryG_T
Cuts at: 1203 2361 2748 5098 6075 1203
Size: 1158 387 2350 977 1327
Fragments arranged by size:
2350 1327 1158 977 387
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 6167
AlwNI CAG_nnn'CTG
Cuts at: 2293 2452 2596 2728 3942 5514 2293
Size: 159 144 132 1214 1572 2978
Fragments arranged by size:
2978 1572 1214 159 144 132
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 3508 3734 3886 5995 1493
Size: 2015 226 152 2109 1697
Fragments arranged by size:
2109 2015 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 3524 1870
Size: 73 822 759 4545
Fragments arranged by size:
4545 822 759 73
AvrII C'CTAG_G
Cuts at: 2923 2923
Size: 6199
BamHI G'GATC_C
Cuts at: 3457 3529 3896 3457
Size: 72 367 5760
Fragments arranged by size:
5760 367 72
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 3520 4975 618
Size: 325 217 627 584 1149 1455 1842
Fragments arranged by size:
1842 1455 1149 627 584 325 217
BanII G_rGCy'C
Cuts at: 1391 2828 3518 1391
Size: 1437 690 4072
Fragments arranged by size:
4072 1437 690
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2641 3069 928
Size: 971 742 428 4058
Fragments arranged by size:
4058 971 742 428
BbvCI CC'TCA_GC
Cuts at: 3158 3158
Size: 6199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2876 2910 4390 4424 1903
Size: 34 939 34 1480 34 3678
Fragments arranged by size:
3678 1480 939 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2528 4091 5301 2528
Size: 1563 1210 3426
Fragments arranged by size:
3426 1563 1210
BclI T'GATC_A
Cuts at: 1548 2941 1548
Size: 1393 4806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2646 3145 4260 5416 1790
Size: 586 270 499 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 586 499 270
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 3930 4888
Size: 416 53 83 399 24 1377 1136 958
Cuts at: 4888 442
Size: 1753
Fragments arranged by size:
1753 1377 1136 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 4859 1239
Size: 1190 2430 2579
Fragments arranged by size:
2579 2430 1190
Bpu10I CC'TnA_GC
Cuts at: 3158 3158
Size: 6199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2950 3318 4324 5189 5487 2950
Size: 368 1006 865 298 3662
Fragments arranged by size:
3662 1006 865 368 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4862 882
Size: 617 3363 2219
Fragments arranged by size:
3363 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2080 3990 493
Size: 711 876 1910 2702
Fragments arranged by size:
2702 1910 876 711
BsaBI GATnn'nnATC
Cuts at: 2516 2946 6193 2516
Size: 430 3247 2522
Fragments arranged by size:
3247 2522 430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3096 4006 4388 5023
Size: 53 83 186 936 1563 910 382 635
Cuts at: 5023 275
Size: 1451
Fragments arranged by size:
1563 1451 936 910 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2849 2879 1178
Size: 30 165 30 1446 30 4498
Fragments arranged by size:
4498 1446 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 2903 3355 1186
Size: 1684 33 452 4030
Fragments arranged by size:
4030 1684 452 33
BseYI C'CCAG_C
Cuts at: 2068 2279 2286 2602 5402 5898 2068
Size: 211 7 316 2800 496 2369
Fragments arranged by size:
2800 2369 496 316 211 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5626
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 3649 4410 5438 1540
Size: 275 186 136 1512 761 1028 2301
Fragments arranged by size:
2301 1512 1028 761 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2238 3321 4260 4345 5416 2238
Size: 1083 939 85 1071 3021
Fragments arranged by size:
3021 1083 1071 939 85
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2376 2646 2828 3145 3321 3518
Size: 399 448 138 270 182 317 176 197
Cuts at: 3518 4260 4345 5416 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 448 399 317 270 197
182 176 138 85
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 2142 4088 5818 2142
Size: 1946 1730 2523
Fragments arranged by size:
2523 1946 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2736 3554 844
Size: 1286 606 818 3489
Fragments arranged by size:
3489 1286 818 606
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4694 4868 65
Size: 1183 3446 174 1396
Fragments arranged by size:
3446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 4843 5030 1700
Size: 110 377 2656 187 2869
Fragments arranged by size:
2869 2656 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 2556 96
Size: 2460 3739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 3008 4259 5271 3008
Size: 1251 1012 3936
Fragments arranged by size:
3936 1251 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 794
Size: 1502 4697
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 3381 3457 3529 3896 4281 4298 5750 5836
Size: 1409 76 72 367 385 17 1452 86
Cuts at: 5836 5848 6194 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1409 385 367 346 86 76
72 17 12
BstZ17I GTA'TAC
Cuts at: 2532 2532
Size: 6199
BtgI C'CryG_G
Cuts at: 513 1065 2126 513
Size: 552 1061 4586
Fragments arranged by size:
4586 1061 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3315 487
Size: 683 56 2089 3371
Fragments arranged by size:
3371 2089 683 56
BtsI GCAGTG_nn'
Cuts at: 3715 4509 4529 3715
Size: 794 20 5385
Fragments arranged by size:
5385 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DraIII CAC_nnn'GTG
Cuts at: 2296 2296
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2620 2914 3646
Size: 54 1425 50 275 18 790 294 732
Cuts at: 3646 4535 6164 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 790 732 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4129 1353
Size: 2776 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2785 4786 5160 5306 387
Size: 1064 138 1196 2001 374 146 1280
Fragments arranged by size:
2001 1280 1196 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2708 4262 4859 5646 5914 1239
Size: 393 797 279 1554 597 787 268 1524
Fragments arranged by size:
1554 1524 797 787 597 393 279 268
EcoO109I rG'GnC_Cy
Cuts at: 2431 2725 3013 3306 2431
Size: 294 288 293 5324
Fragments arranged by size:
5324 294 293 288
EcoRI G'AATT_C
Cuts at: 3508 3886 3508
Size: 378 5821
FspI TGC'GCA
Cuts at: 2246 4705 2246
Size: 2459 3740
HaeII r_GCGC'y
Cuts at: 1678 2275 2286 3200 5346 1678
Size: 597 11 914 2146 2531
Fragments arranged by size:
2531 2146 914 597 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 4840 4872 4914
Size: 32 135 32 837 32 2399 32 42
Cuts at: 4914 4946 1373
Size: 32 2626
Fragments arranged by size:
2626 2399 837 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3080 3206 3244 748
Size: 2332 126 38 3703
Fragments arranged by size:
3703 2332 126 38
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 5313 5497 1305
Size: 1011 2997 184 2007
Fragments arranged by size:
2997 2007 1011 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2622 10
Size: 54 1425 343 790 3587
Fragments arranged by size:
3587 1425 790 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 3170 4157 4516 4675 518
Size: 648 1330 674 987 359 159 2042
Fragments arranged by size:
2042 1330 987 674 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2789 3615 4292 5440 5685 1606
Size: 494 689 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 689 677 494 245
NaeI GCC'GGC
Cuts at: 2189 2189
Size: 6199
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5647
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NgoMIV G'CCGG_C
Cuts at: 2187 2187
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 2365 2752 3557 5102 1132
Size: 97 1136 387 805 1545 2229
Fragments arranged by size:
2229 1545 1136 805 387 97
PasI CC'CwG_GG
Cuts at: 1348 2376 2955 1348
Size: 1028 579 4592
Fragments arranged by size:
4592 1028 579
PciI A'CATG_T
Cuts at: 2361 2748 5098 2361
Size: 387 2350 3462
Fragments arranged by size:
3462 2350 387
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2293 2629 1108
Size: 1185 336 4678
Fragments arranged by size:
4678 1185 336
PfoI T'CCnGG_A
Cuts at: 2427 2427
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 4268 4300 5781 5813 2849
Size: 32 1387 32 1481 32 3235
Fragments arranged by size:
3235 1481 1387 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2725 2431
Size: 294 5905
PshAI GACnn'nnGTC
Cuts at: 1613 2087 1613
Size: 474 5725
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PstI C_TGCA'G
Cuts at: 830 2664 2934 3551 830
Size: 1834 270 617 3478
Fragments arranged by size:
3478 1834 617 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
PvuII CAG'CTG
Cuts at: 2789 2789
Size: 6199
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SanDI GG'GwC_CC
Cuts at: 2431 2431
Size: 6199
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SexAI A'CCwGG_T
Cuts at: 2292 2292
Size: 6199
SfcI C'TryA_G
Cuts at: 826 1045 2660 2930 3136 3547 4682 5363 5554
Size: 219 1615 270 206 411 1135 681 191
Cuts at: 5554 826
Size: 1471
Fragments arranged by size:
1615 1471 1135 681 411 270 219 206
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2965 3083 3333 4303 5204 5466
Size: 197 926 1022 118 250 970 901 262
Cuts at: 5466 820
Size: 1553
Fragments arranged by size:
1553 1022 970 926 901 262 250 197
118
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 2928 3074 1517
Size: 1095 316 146 4642
Fragments arranged by size:
4642 1095 316 146
StyI C'CwwG_G
Cuts at: 513 1065 2607 2923 3471 3638 513
Size: 552 1542 316 548 167 3074
Fragments arranged by size:
3074 1542 552 548 316 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 2368 3336 4226 4243 4396 4581
Size: 1255 779 9 968 890 17 153 185
Cuts at: 4581 5026 325
Size: 445 1498
Fragments arranged by size:
1498 1255 968 890 779 445 185 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2556 2751 4445
Size: 274 80 33 51 1443 579 195 1694
Cuts at: 4445 6157 96
Size: 1712 138
Fragments arranged by size:
1712 1694 1443 579 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 3000 4328 3000
Size: 1328 4871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SfiI SfoI
SrfI SwaI XcmI