Restriction map for
pFN21AE5649
(Circular) (Six-base) MAPSORT of: pFN21AE5649.seq Check: 7705 from: 1 to: 6187
pFN21AE5649 6187 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2700 2700
Size: 6187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4250 5634 5902 1632
Size: 2618 1384 268 1917
Fragments arranged by size:
2618 1917 1384 268
AfeI AGC'GCT
Cuts at: 2195 2195
Size: 6187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 3420 3433 5086 6063 1203
Size: 2217 13 1653 977 1327
Fragments arranged by size:
2217 1653 1327 977 13
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 3173 1166
Size: 2007 4180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2948 1184
Size: 1764 4423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 6155
AlwNI CAG_nnn'CTG
Cuts at: 2725 3930 5502 2725
Size: 1205 1572 3410
Fragments arranged by size:
3410 1572 1205
ApaI G_GGCC'C
Cuts at: 2229 2229
Size: 6187
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 3496 3722 3874 5983 1493
Size: 2003 226 152 2109 1697
Fragments arranged by size:
2109 2003 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BamHI G'GATC_C
Cuts at: 2782 3517 3884 2782
Size: 735 367 5085
Fragments arranged by size:
5085 735 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2498 2909 3167 3508 4963
Size: 325 217 627 711 411 258 341 1455
Cuts at: 4963 618
Size: 1842
Fragments arranged by size:
1842 1455 711 627 411 341 325 258
217
BanII G_rGCy'C
Cuts at: 1391 2229 3194 3351 3506 1391
Size: 838 965 157 155 4072
Fragments arranged by size:
4072 965 838 157 155
BbeI G_GCGC'C
Cuts at: 2502 2502
Size: 6187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 928
Size: 971 427 4789
Fragments arranged by size:
4789 971 427
BbvCI CC'TCA_GC
Cuts at: 2289 2289
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4378 4412 1903
Size: 34 50 34 2357 34 3678
Fragments arranged by size:
3678 2357 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4798 136
Size: 107 122 71 631 605 337 2789 1525
Fragments arranged by size:
2789 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2492 2912 3170 3297 4248 5404 1790
Size: 439 263 420 258 127 951 1156 2573
Fragments arranged by size:
2573 1156 951 439 420 263 258 127
BmgBI CAC'GTC
Cuts at: 1318 2640 3097 3421 1318
Size: 1322 457 324 4084
Fragments arranged by size:
4084 1322 457 324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 3204 3394
Size: 416 53 83 399 24 921 866 190
Cuts at: 3394 3918 4876 442
Size: 524 958 1753
Fragments arranged by size:
1753 958 921 866 524 416 399 190
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 4847 1239
Size: 1517 2091 2579
Fragments arranged by size:
2579 2091 1517
Bpu10I CC'TnA_GC
Cuts at: 2289 2525 2289
Size: 236 5951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3374 4312 5177 5475 3374
Size: 938 865 298 4086
Fragments arranged by size:
4086 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2909 3198 4850 882
Size: 617 1410 289 1652 2219
Fragments arranged by size:
2219 1652 1410 617 289
BsaAI yAC'GTr
Cuts at: 493 1204 3436 3978 493
Size: 711 2232 542 2702
Fragments arranged by size:
2702 2232 711 542
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2478 2499 3994 4376
Size: 53 83 186 936 945 21 1495 382
Cuts at: 4376 5011 275
Size: 635 1451
Fragments arranged by size:
1495 1451 945 936 635 382 186 83
53 21
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5962
Fragments arranged by size:
5962 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2456 2471 2849 2852 3005 1186
Size: 1120 150 15 378 3 153 4368
Fragments arranged by size:
4368 1120 378 153 150 15 3
BseYI C'CCAG_C
Cuts at: 3187 3243 5390 5886 3187
Size: 56 2147 496 3488
Fragments arranged by size:
3488 2147 496 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5614
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2766 3351 4248 4333 5404 2766
Size: 585 897 85 1071 3549
Fragments arranged by size:
3549 1071 897 585 85
BsiWI C'GTAC_G
Cuts at: 2774 2774
Size: 6187
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
BsmBI CGTCTCn'nnnn_
Cuts at: 3087 3087
Size: 6187
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2492 2766 2912 3170 3194 3297
Size: 399 439 263 274 146 258 24 103
Cuts at: 3297 3351 3506 4248 4333 5404 1391
Size: 54 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 274 263 258
155 146 103 85 54 24
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 2178 2955 4076 5806 2178
Size: 777 1121 1730 2559
Fragments arranged by size:
2559 1730 1121 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2700 3542 844
Size: 1307 549 842 3489
Fragments arranged by size:
3489 1307 842 549
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2120 4682 4856 65
Size: 1183 872 2562 174 1396
Fragments arranged by size:
2562 1396 1183 872 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3018 4831 5018 1700
Size: 110 1208 1813 187 2869
Fragments arranged by size:
2869 1813 1208 187 110
BsrGI T'GTAC_A
Cuts at: 96 2166 96
Size: 2070 4117
BssHII G'CGCG_C
Cuts at: 1925 2371 1925
Size: 446 5741
BssSI C'ACGA_G
Cuts at: 3074 4247 5259 3074
Size: 1173 1012 4002
Fragments arranged by size:
4002 1173 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2221 2758 3089 3112 3436 2221
Size: 537 331 23 324 4972
Fragments arranged by size:
4972 537 331 324 23
BstYI r'GATC_y
Cuts at: 1972 2782 3078 3517 3884 4269 4286 5738 5824
Size: 810 296 439 367 385 17 1452 86
Cuts at: 5824 5836 6182 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 810 439 385 367 346 296
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2014 3162 3408 2014
Size: 1148 246 4793
Fragments arranged by size:
4793 1148 246
BtgI C'CryG_G
Cuts at: 513 1065 2649 2795 513
Size: 552 1584 146 3905
Fragments arranged by size:
3905 1584 552 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2383 487
Size: 683 56 1157 4291
Fragments arranged by size:
4291 1157 683 56
BtsI GCAGTG_nn'
Cuts at: 3072 3703 4497 4517 3072
Size: 631 794 20 4742
Fragments arranged by size:
4742 794 631 20
ClaI AT'CG_AT
Cuts at: 1554 2508 1554
Size: 954 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3336 3634 4523
Size: 54 1425 50 275 18 1506 298 889
Cuts at: 4523 6152 8
Size: 1629 43
Fragments arranged by size:
1629 1506 1425 889 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2680 2903 3272 3362 4117 1353
Size: 728 599 223 369 90 755 3423
Fragments arranged by size:
3423 755 728 599 369 223 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3374 4774 5148 5294 387
Size: 1064 138 1785 1400 374 146 1280
Fragments arranged by size:
1785 1400 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3349 3349
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2756 4250 4847 5634 5902 1239
Size: 393 1124 1494 597 787 268 1524
Fragments arranged by size:
1524 1494 1124 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3161 3161
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 2111 2225 2226 2111
Size: 114 1 6072
Fragments arranged by size:
6072 114 1
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 2612 4693 2612
Size: 2081 4106
HaeII r_GCGC'y
Cuts at: 1678 2197 2392 2502 5334 1678
Size: 519 195 110 2832 2531
Fragments arranged by size:
2832 2531 519 195 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 3084 3116 4828
Size: 32 135 32 1013 32 467 32 1712
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1712 1013 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 3531 3777 669
Size: 988 281 705 888 246 3079
Fragments arranged by size:
3079 988 888 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2498 2498
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3246 5301 5485 1305
Size: 1941 2055 184 2007
Fragments arranged by size:
2055 2007 1941 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3338 10
Size: 54 1425 343 1506 2859
Fragments arranged by size:
2859 1506 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 3173 3371 3419 4145 4504 4663
Size: 648 1659 348 198 48 726 359 159
Cuts at: 4663 518
Size: 2042
Fragments arranged by size:
2042 1659 726 648 359 348 198 159
48
MspA1I CmG'CkG
Cuts at: 1606 2301 3603 4280 5428 5673 1606
Size: 695 1302 677 1148 245 2120
Fragments arranged by size:
2120 1302 1148 695 677 245
NarI GG'CG_CC
Cuts at: 2499 2499
Size: 6187
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2830 3545 5090 1132
Size: 97 1601 715 1545 2229
Fragments arranged by size:
2229 1601 1545 715 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2107 2451 2107
Size: 344 5843
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PmlI CAC'GTG
Cuts at: 3436 3436
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2111
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspOMI G'GGCC_C
Cuts at: 2225 2225
Size: 6187
PstI C_TGCA'G
Cuts at: 830 3539 830
Size: 2709 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SacI G_AGCT'C
Cuts at: 3351 3351
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 2111 2111
Size: 6187
SapI GCTCTTCn'nnn_
Cuts at: 2081 2081
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SexAI A'CCwGG_T
Cuts at: 2874 2874
Size: 6187
SfcI C'TryA_G
Cuts at: 826 1045 3535 4670 5351 5542 826
Size: 219 2490 1135 681 191 1471
Fragments arranged by size:
2490 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2500 2500
Size: 6187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3389 4291 5192 5454 820
Size: 197 926 1446 902 901 262 1553
Fragments arranged by size:
1553 1446 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 2830 3545 1132
Size: 1698 715 3774
Fragments arranged by size:
3774 1698 715
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 3333 1517
Size: 1197 619 4371
Fragments arranged by size:
4371 1197 619
StyI C'CwwG_G
Cuts at: 513 1065 2979 3626 513
Size: 552 1914 647 3074
Fragments arranged by size:
3074 1914 647 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2979 4214 4231 4384 4569 5014 325
Size: 1255 1399 1235 17 153 185 445 1498
Fragments arranged by size:
1498 1399 1255 1235 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 4433 6145
Size: 274 80 33 51 1443 189 2267 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
2267 1712 1443 274 189 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 2330 1316
Size: 1014 5173
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 2897 3320 4316 2897
Size: 423 996 4768
Fragments arranged by size:
4768 996 423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PsrI PvuII RsrII SacII SfiI SrfI SwaI XcmI