Restriction map for
pFN21AE5646
(Circular) (Six-base) MAPSORT of: pFN21AE5646.seq Check: 8727 from: 1 to: 6181
pFN21AE5646 6181 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2040 3991 278
Size: 53 83 186 936 504 1951 2468
Fragments arranged by size:
2468 1951 936 504 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3204 4244 5628 5896 1632
Size: 1572 1040 1384 268 1917
Fragments arranged by size:
1917 1572 1384 1040 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 2938 5080 6057 1203
Size: 1735 2142 977 1327
Fragments arranged by size:
2142 1735 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 4910 4910
Size: 6181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6181
AlwNI CAG_nnn'CTG
Cuts at: 2180 3924 5496 2180
Size: 1744 1572 2865
Fragments arranged by size:
2865 1744 1572
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 2083 2864 3490 3716 3868 5977 1493
Size: 590 781 626 226 152 2109 1697
Fragments arranged by size:
2109 1697 781 626 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2421 2522 4735 160
Size: 872 1389 101 2213 1606
Fragments arranged by size:
2213 1606 1389 872 101
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 3342 3506 1870
Size: 73 1399 164 4545
Fragments arranged by size:
4545 1399 164 73
BamHI G'GATC_C
Cuts at: 3511 3878 3511
Size: 367 5814
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3502 4957 618
Size: 325 217 627 1715 1455 1842
Fragments arranged by size:
1842 1715 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3500 1391
Size: 2109 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2837 3126 928
Size: 971 938 289 3983
Fragments arranged by size:
3983 971 938 289
BbvCI CC'TCA_GC
Cuts at: 2619 2619
Size: 6181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4372 4406 1903
Size: 34 2435 34 3678
Fragments arranged by size:
3678 2435 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3076 4073 5283 3076
Size: 997 1210 3974
Fragments arranged by size:
3974 1210 997
BclI T'GATC_A
Cuts at: 1548 2221 2308 1548
Size: 673 87 5421
Fragments arranged by size:
5421 673 87
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3394 6176 3394
Size: 2782 3399
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 4242 5398 1790
Size: 2452 1156 2573
Fragments arranged by size:
2573 2452 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2744 3912 4870
Size: 416 53 83 399 24 1327 1168 958
Cuts at: 4870 442
Size: 1753
Fragments arranged by size:
1753 1327 1168 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2861
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2700 4841 1239
Size: 800 661 2141 2579
Fragments arranged by size:
2579 2141 800 661
Bpu10I CC'TnA_GC
Cuts at: 2619 2619
Size: 6181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4306 5171 5469 4306
Size: 865 298 5018
Fragments arranged by size:
5018 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2420 2682 3303 3420 4844 882
Size: 617 516 405 262 621 117 1424 2219
Fragments arranged by size:
2219 1424 621 617 516 405 262 117
BsaAI yAC'GTr
Cuts at: 493 1204 3972 493
Size: 711 2768 2702
Fragments arranged by size:
2768 2702 711
BsaBI GATnn'nnATC
Cuts at: 2766 6175 2766
Size: 3409 2772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3988 4370 5005
Size: 53 83 186 936 504 1951 382 635
Cuts at: 5005 275
Size: 1451
Fragments arranged by size:
1951 1451 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4614 5012 5286 5433 5844 4614
Size: 398 274 147 411 4951
Fragments arranged by size:
4951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5956
Fragments arranged by size:
5956 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 1186
Size: 1019 5162
BseYI C'CCAG_C
Cuts at: 5384 5880 5384
Size: 496 5685
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5608
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 3631 4392 5420 1540
Size: 275 186 68 1562 761 1028 2301
Fragments arranged by size:
2301 1562 1028 761 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 4242 4327 5398 4242
Size: 85 1071 5025
Fragments arranged by size:
5025 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 3692 3785 2894
Size: 798 93 5290
Fragments arranged by size:
5290 798 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3500 4242 4327 5398 1391
Size: 399 1710 742 85 1071 2174
Fragments arranged by size:
2174 1710 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 4070 5800 4070
Size: 1730 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3536 844
Size: 2692 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4676 4850 65
Size: 1183 3428 174 1396
Fragments arranged by size:
3428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4825 5012 1700
Size: 110 3015 187 2869
Fragments arranged by size:
3015 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2052 2137 96
Size: 1956 85 4140
Fragments arranged by size:
4140 1956 85
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6181
BstXI CCAn_nnnn'nTGG
Cuts at: 2216 2216
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2318 2767 3394 3511 3878 4263 4280 5732
Size: 346 449 627 117 367 385 17 1452
Cuts at: 5732 5818 5830 6176 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 627 449 385 367 346 346
117 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 2289 513
Size: 552 939 181 104 4405
Fragments arranged by size:
4405 939 552 181 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 2311 3697 4491 4511 2311
Size: 1386 794 20 3981
Fragments arranged by size:
3981 1386 794 20
ClaI AT'CG_AT
Cuts at: 1554 2533 1554
Size: 979 5202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3030 3065 529
Size: 35 2466 35 3645
Fragments arranged by size:
3645 2466 35 35
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2839 5188 809
Size: 507 1523 2349 1802
Fragments arranged by size:
2349 1802 1523 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2182 2206
Size: 54 1425 50 275 18 236 116 24
Cuts at: 2206 3355 3628 4517 6146 8
Size: 1149 273 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 275 273 236 116
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2066 3628 1537
Size: 275 254 1562 4090
Fragments arranged by size:
4090 1562 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 3180 4111 1353
Size: 1827 931 3423
Fragments arranged by size:
3423 1827 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4768 5142 5288 387
Size: 1064 138 3179 374 146 1280
Fragments arranged by size:
3179 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2700 3204 4244 4841 5628 5896
Size: 393 407 661 504 1040 597 787 268
Cuts at: 5896 1239
Size: 1524
Fragments arranged by size:
1524 1040 787 661 597 504 407 393
268
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
EcoRV GAT'ATC
Cuts at: 2464 3235 2464
Size: 771 5410
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 5328 1678
Size: 3650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 3113 3145 4822
Size: 32 135 32 612 32 897 32 1677
Cuts at: 4822 4854 4896 4928 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1677 897 612 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 3525 3771 669
Size: 988 281 499 1088 246 3079
Fragments arranged by size:
3079 1088 988 499 281 246
HindIII A'AGCT_T
Cuts at: 748 2230 748
Size: 1482 4699
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2208 3357 10
Size: 54 1425 343 376 1149 2834
Fragments arranged by size:
2834 1425 1149 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4139 4498 4657 518
Size: 648 2973 359 159 2042
Fragments arranged by size:
2973 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2618 3597 4274 5422 5667 1606
Size: 1012 979 677 1148 245 2120
Fragments arranged by size:
2120 1148 1012 979 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2289 513
Size: 552 939 285 4405
Fragments arranged by size:
4405 939 552 285
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 2942 3211 3539 5084 1132
Size: 97 1713 269 328 1545 2229
Fragments arranged by size:
2229 1713 1545 328 269 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 2938 5080 2938
Size: 2142 4039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6181
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 5763 5795 4250
Size: 32 1481 32 4636
Fragments arranged by size:
4636 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PstI C_TGCA'G
Cuts at: 830 3533 830
Size: 2703 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
PvuII CAG'CTG
Cuts at: 2618 2618
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 4429 6141 4429
Size: 1712 4469
SfcI C'TryA_G
Cuts at: 826 1045 2172 3238 3529 4664 5345 5536 826
Size: 219 1127 1066 291 1135 681 191 1471
Fragments arranged by size:
1471 1135 1127 1066 681 291 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3508 1872
Size: 1636 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3342 4285 5186 5448 820
Size: 197 926 1399 943 901 262 1553
Fragments arranged by size:
1553 1399 943 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 3539 1132
Size: 2407 3774
SspI AAT'ATT
Cuts at: 5 52 2111 2781 4105 5
Size: 47 2059 670 1324 2081
Fragments arranged by size:
2081 2059 1324 670 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6181
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 3620 513
Size: 552 939 285 1331 3074
Fragments arranged by size:
3074 1331 939 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3327 3444 4208 4225 4378 4563 5008
Size: 1255 1747 117 764 17 153 185 445
Cuts at: 5008 325
Size: 1498
Fragments arranged by size:
1747 1498 1255 764 445 185 153 117
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2137 2725
Size: 274 80 33 51 1443 75 85 588
Cuts at: 2725 4427 6139 96
Size: 1702 1712 138
Fragments arranged by size:
1712 1702 1443 588 274 138 85 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2170 2202 6141 6173 2170
Size: 32 3939 32 2178
Fragments arranged by size:
3939 2178 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2039 1316
Size: 723 5458
XbaI T'CTAG_A
Cuts at: 2350 2786 3517 2350
Size: 436 731 5014
Fragments arranged by size:
5014 731 436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 2378
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 3342 1943
Size: 1399 4782
XmaI C'CCGG_G
Cuts at: 1870 3506 1870
Size: 1636 4545
XmnI GAAnn'nnTTC
Cuts at: 2894 4310 2894
Size: 1416 4765
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2038 3989 276
Size: 53 83 186 936 504 1951 2468
Fragments arranged by size:
2468 1951 936 504 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI