Restriction map for
pFN21AE5645
(Circular) (Six-base) MAPSORT of: pFN21AE5645.seq Check: 3633 from: 1 to: 6145
pFN21AE5645 6145 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2000 2000
Size: 6145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3018 3380 4208 5592 5860 1632
Size: 1386 362 828 1384 268 1917
Fragments arranged by size:
1917 1386 1384 828 362 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 2628 1166
Size: 1462 4683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2363 2395 2363
Size: 32 6113
AlwNI CAG_nnn'CTG
Cuts at: 3888 5460 3888
Size: 1572 4573
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2862 4699 160
Size: 872 1830 1837 1606
Fragments arranged by size:
1837 1830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2228 3470 1870
Size: 73 285 1242 4545
Fragments arranged by size:
4545 1242 285 73
BamHI G'GATC_C
Cuts at: 3002 3475 3842 3002
Size: 473 367 5305
Fragments arranged by size:
5305 473 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 3466 4921 618
Size: 325 217 627 1457 222 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 222 217
BanII G_rGCy'C
Cuts at: 1391 3024 3150 3464 1391
Size: 1633 126 314 4072
Fragments arranged by size:
4072 1633 314 126
BbeI G_GCGC'C
Cuts at: 3248 3248
Size: 6145
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2354 3025 928
Size: 971 231 224 671 4048
Fragments arranged by size:
4048 971 671 231 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2738 2772 4336 4370 1903
Size: 34 801 34 1564 34 3678
Fragments arranged by size:
3678 1564 801 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 3063 1548
Size: 1515 4630
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 2730 3562 2730
Size: 832 5313
Bme1580I G_kGCm'C
Cuts at: 1790 3271 4206 5362 1790
Size: 1481 935 1156 2573
Fragments arranged by size:
2573 1481 1156 935
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3876 4834 442
Size: 416 53 83 399 24 2459 958 1753
Fragments arranged by size:
2459 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 4805 1239
Size: 1124 2442 2579
Fragments arranged by size:
2579 2442 1124
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 6145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2265 3244 4270 5135 5433 2265
Size: 979 1026 865 298 2977
Fragments arranged by size:
2977 1026 979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 2247 6139 2247
Size: 3892 2253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3245 3952 4334 4969
Size: 53 83 186 936 1712 707 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1712 1451 936 707 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4578 4976 5250 5397 5808 4578
Size: 398 274 147 411 4915
Fragments arranged by size:
4915 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 1178
Size: 30 165 30 1080 30 4810
Fragments arranged by size:
4810 1080 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 3251 3254 1186
Size: 1321 744 3 4077
Fragments arranged by size:
4077 1321 744 3
BseYI C'CCAG_C
Cuts at: 3335 5348 5844 3335
Size: 2013 496 3636
Fragments arranged by size:
3636 2013 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 1163
Size: 573 337 5235
Fragments arranged by size:
5235 573 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2033 2625 2722 4206 4291 5362 2033
Size: 592 97 1484 85 1071 2816
Fragments arranged by size:
2816 1484 1071 592 97 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
BsmBI CGTCTCn'nnnn_
Cuts at: 3340 3340
Size: 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2625 2722 3024 3150 3271 3464
Size: 399 243 592 97 302 126 121 193
Cuts at: 3464 4206 4291 5362 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 399 302 243 193
126 121 97 85
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2367 3413 3500 844
Size: 1156 367 1046 87 3489
Fragments arranged by size:
3489 1156 1046 367 87
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2308 2322 96
Size: 2212 14 3919
Fragments arranged by size:
3919 2212 14
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 2548 4949 2548
Size: 2401 3744
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 3002 3475 3842 4227 4244 5696 5782 5794
Size: 1030 473 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1030 473 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2848 3150 513
Size: 552 939 844 302 3508
Fragments arranged by size:
3508 939 844 552 302
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2400 487
Size: 683 56 1174 4232
Fragments arranged by size:
4232 1174 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3661 4455 4475 2992
Size: 669 794 20 4662
Fragments arranged by size:
4662 794 669 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2995 3030 529
Size: 35 2431 35 3644
Fragments arranged by size:
3644 2431 35 35
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2975 5152 809
Size: 507 1659 2177 1802
Fragments arranged by size:
2177 1802 1659 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2561 3592 4481
Size: 54 1425 50 275 18 731 1031 889
Cuts at: 4481 6110 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1031 889 731 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2478 3338 3384 3418 4075 1353
Size: 1125 860 46 34 657 3423
Fragments arranged by size:
3423 1125 860 657 46 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 3018 3380 4208 4805 5592 5860
Size: 393 731 655 362 828 597 787 268
Cuts at: 5860 1239
Size: 1524
Fragments arranged by size:
1524 828 787 731 655 597 393 362
268
EcoO109I rG'GnC_Cy
Cuts at: 2233 2233
Size: 6145
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2574 2574
Size: 6145
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 3248 5292 1678
Size: 1570 2044 2531
Fragments arranged by size:
2531 2044 1570
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2483 2515 4786
Size: 32 135 32 493 32 386 32 2271
Cuts at: 4786 4818 4860 4892 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2271 493 386 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 3489 3735 669
Size: 988 281 454 1097 246 3079
Fragments arranged by size:
3079 1097 988 454 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KasI G'GCGC_C
Cuts at: 3244 3244
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2533 5259 5443 1305
Size: 1228 2726 184 2007
Fragments arranged by size:
2726 2007 1228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2563 10
Size: 54 1425 343 731 3592
Fragments arranged by size:
3592 1425 731 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2628 2847 4103 4462 4621 518
Size: 648 1462 219 1256 359 159 2042
Fragments arranged by size:
2042 1462 1256 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 3561 4238 5386 5631 1606
Size: 1955 677 1148 245 2120
Fragments arranged by size:
2120 1955 1148 677 245
NarI GG'CG_CC
Cuts at: 3245 3245
Size: 6145
NcoI C'CATG_G
Cuts at: 513 1065 2004 2848 3150 513
Size: 552 939 844 302 3508
Fragments arranged by size:
3508 939 844 552 302
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 2398 3503 5048 1132
Size: 97 1169 1105 1545 2229
Fragments arranged by size:
2229 1545 1169 1105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 2427 3715 2427
Size: 1288 4857
PspXI vC'TCGA_Gb
Cuts at: 2228 2228
Size: 6145
PstI C_TGCA'G
Cuts at: 830 3410 3497 830
Size: 2580 87 3478
Fragments arranged by size:
3478 2580 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SapI GCTCTTCn'nnn_
Cuts at: 3384 3384
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2298 2298
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 3406 3493 4628 5309 5500 826
Size: 219 2361 87 1135 681 191 1471
Fragments arranged by size:
2361 1471 1135 681 219 191 87
SfoI GGC'GCC
Cuts at: 3246 3246
Size: 6145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 2280 3259 4249 5150 5412
Size: 197 926 285 52 979 990 901 262
Cuts at: 5412 820
Size: 1553
Fragments arranged by size:
1553 990 979 926 901 285 262 197
52
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2848 3150 3584 513
Size: 552 939 844 302 434 3074
Fragments arranged by size:
3074 939 844 552 434 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4172 4189 4342 4527 4972 325
Size: 1255 2592 17 153 185 445 1498
Fragments arranged by size:
2592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2308 2322 3314
Size: 274 80 33 51 1443 331 14 992
Cuts at: 3314 4391 6103 96
Size: 1077 1712 138
Fragments arranged by size:
1712 1443 1077 992 331 274 138 80
51 33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6105 6137 6105
Size: 32 6113
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3308 3481 3308
Size: 173 5972
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2771 2771
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 2228 1943
Size: 285 5860
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 3393 4274 3393
Size: 881 5264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SfiI SrfI SwaI