Restriction map for
pFN21AE5640
(Circular) (Six-base) MAPSORT of: pFN21AE5640.seq Check: 6594 from: 1 to: 6400
pFN21AE5640 6400 bp
With 182 enzymes: SgfI *
December 8, 2014 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3743 1937
Size: 1806 4594
Acc65I G'GTAC_C
Cuts at: 2654 3721 2654
Size: 1067 5333
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 2888 3338 3480 4463 5847 6115 1632
Size: 1076 180 450 142 983 1384 268 1917
Fragments arranged by size:
1917 1384 1076 983 450 268 180 142
AfeI AGC'GCT
Cuts at: 3323 3323
Size: 6400
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 2881 5299 6276 1203
Size: 1678 2418 977 1327
Fragments arranged by size:
2418 1678 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 2971 5129 2971
Size: 2158 4242
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 4990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2162 2625 1184
Size: 978 463 4959
Fragments arranged by size:
4959 978 463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 6368
AlwNI CAG_nnn'CTG
Cuts at: 4143 5715 4143
Size: 1572 4828
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 2869 3709 3935 4087 6196 1493
Size: 1376 840 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 840 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2640 3725 1870
Size: 73 190 507 1085 4545
Fragments arranged by size:
4545 1085 507 190 73
BamHI G'GATC_C
Cuts at: 3549 3730 4097 3549
Size: 181 367 5852
Fragments arranged by size:
5852 367 181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2654 3721 5176 618
Size: 325 217 627 867 1067 1455 1842
Fragments arranged by size:
1842 1455 1067 867 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2132 3542 3719 1391
Size: 741 1410 177 4072
Fragments arranged by size:
4072 1410 741 177
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2778 2936 3035 928
Size: 971 879 158 99 4293
Fragments arranged by size:
4293 971 879 158 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4591 4625 1903
Size: 34 2654 34 3678
Fragments arranged by size:
3678 2654 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 5502 4292
Size: 1210 5190
BclI T'GATC_A
Cuts at: 1548 3627 1548
Size: 2079 4321
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6395 6395
Size: 6400
BlpI GC'TnA_GC
Cuts at: 3817 3817
Size: 6400
Bme1580I G_kGCm'C
Cuts at: 1790 3014 4461 5617 1790
Size: 1224 1447 1156 2573
Fragments arranged by size:
2573 1447 1224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4131 5089 442
Size: 416 53 83 399 24 2714 958 1753
Fragments arranged by size:
2714 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2784 3584 5060 1239
Size: 1545 800 1476 2579
Fragments arranged by size:
2579 1545 1476 800
Bpu10I CC'TnA_GC
Cuts at: 2256 2256
Size: 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 4525 5390 5688 2498
Size: 2027 865 298 3210
Fragments arranged by size:
3210 2027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5063 882
Size: 617 3564 2219
Fragments arranged by size:
3564 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2574 3556 4191 493
Size: 711 1370 982 635 2702
Fragments arranged by size:
2702 1370 982 711 635
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4207 4589 5224 275
Size: 53 83 186 936 2674 382 635 1451
Fragments arranged by size:
2674 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2078 2301 2751 3546 4833 5231 5505 5652 6063
Size: 223 450 795 1287 398 274 147 411
Cuts at: 6063 2078
Size: 2415
Fragments arranged by size:
2415 1287 795 450 411 398 274 223
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2946 2976 3582 3612 1178
Size: 30 165 30 1543 30 606 30 3966
Fragments arranged by size:
3966 1543 606 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2320 2736 1186
Size: 1134 416 4850
Fragments arranged by size:
4850 1134 416
BseYI C'CCAG_C
Cuts at: 5603 6099 5603
Size: 496 5904
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2978 1163
Size: 573 1242 4585
Fragments arranged by size:
4585 1242 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2014 2083 3850 4611 5639 1540
Size: 275 186 13 69 1767 761 1028 2301
Fragments arranged by size:
2301 1767 1028 761 275 186 69 13
BsiHKAI G_wGCw'C
Cuts at: 2132 4461 4546 5617 2132
Size: 2329 85 1071 2915
Fragments arranged by size:
2915 2329 1071 85
BsmI GAATG_Cn'
Cuts at: 2992 3911 4004 2992
Size: 919 93 5388
Fragments arranged by size:
5388 919 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 3014 3542 3719 4461 4546 5617
Size: 399 342 882 528 177 742 85 1071
Cuts at: 5617 1391
Size: 2174
Fragments arranged by size:
2174 1071 882 742 528 399 342 177
85
BspEI T'CCGG_A
Cuts at: 2751 6063 2751
Size: 3312 3088
BspHI T'CATG_A
Cuts at: 4289 6019 4289
Size: 1730 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 3755 844
Size: 1829 1082 3489
Fragments arranged by size:
3489 1829 1082
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 2260 4287 1394
Size: 429 287 150 2027 3507
Fragments arranged by size:
3507 2027 429 287 150
BsrDI GCAATG_nn'
Cuts at: 65 1248 3501 4895 5069 65
Size: 1183 2253 1394 174 1396
Fragments arranged by size:
2253 1396 1394 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 2170 5044 5231 1700
Size: 110 203 157 2874 187 2869
Fragments arranged by size:
2874 2869 203 187 157 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 2178 2180 1925
Size: 253 2 6145
Fragments arranged by size:
6145 253 2
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6400
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstXI CCAn_nnnn'nTGG
Cuts at: 2834 2834
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 3066 3439 3549 3730 4097 4482 4499 5951
Size: 1094 373 110 181 367 385 17 1452
Cuts at: 5951 6037 6049 6395 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1094 385 373 367 346 181
110 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2118 2118
Size: 6400
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 2314 3916 4710 4730 2314
Size: 1602 794 20 3984
Fragments arranged by size:
3984 1602 794 20
ClaI AT'CG_AT
Cuts at: 1554 2550 1554
Size: 996 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5407 809
Size: 507 4091 1802
Fragments arranged by size:
4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2281 3847
Size: 54 1425 50 275 18 181 270 1566
Cuts at: 3847 4736 6365 8
Size: 889 1629 43
Fragments arranged by size:
1629 1566 1425 889 275 270 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2011 3847 1537
Size: 275 199 1836 4090
Fragments arranged by size:
4090 1836 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 3254 3656 4330 1353
Size: 1901 402 674 3423
Fragments arranged by size:
3423 1901 674 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4987 5361 5507 387
Size: 1064 138 3398 374 146 1280
Fragments arranged by size:
3398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2130 2130
Size: 6400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2708 2784 2888 3338 3480 3584 4463
Size: 393 1076 76 104 450 142 104 879
Cuts at: 4463 5060 5847 6115 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1076 879 787 597 450 393 268
142 104 104 76
EcoNI CCTnn'n_nnAGG
Cuts at: 3206 3206
Size: 6400
EcoO109I rG'GnC_Cy
Cuts at: 2188 2188
Size: 6400
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
EcoRV GAT'ATC
Cuts at: 2569 2809 2569
Size: 240 6160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3328 3403 3328
Size: 75 6325
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 3325 5547 1678
Size: 1647 2222 2531
Fragments arranged by size:
2531 2222 1647
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2231 2263 2957
Size: 32 135 32 476 32 151 32 694
Cuts at: 2957 2989 5041 5073 5115 5147 1373
Size: 32 2052 32 42 32 2626
Fragments arranged by size:
2626 2052 694 476 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 3744 3990 669
Size: 988 281 1027 779 246 3079
Fragments arranged by size:
3079 1027 988 779 281 246
HindIII A'AGCT_T
Cuts at: 748 3080 748
Size: 2332 4068
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KpnI G_GTAC'C
Cuts at: 2658 3725 2658
Size: 1067 5333
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3431 5514 5698 1305
Size: 2126 2083 184 2007
Fragments arranged by size:
2126 2083 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4578
Fragments arranged by size:
4578 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2397 2576 3167 4358 4717 4876 518
Size: 648 1231 179 591 1191 359 159 2042
Fragments arranged by size:
2042 1231 1191 648 591 359 179 159
MspA1I CmG'CkG
Cuts at: 1606 3816 4493 5641 5886 1606
Size: 2210 677 1148 245 2120
Fragments arranged by size:
2210 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2172 2172
Size: 6400
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5848
NdeI CA'TA_TG
Cuts at: 387 2647 387
Size: 2260 4140
NgoMIV G'CCGG_C
Cuts at: 2170 2170
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 2885 3758 5303 1132
Size: 97 1656 873 1545 2229
Fragments arranged by size:
2229 1656 1545 873 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6400
PciI A'CATG_T
Cuts at: 2881 5299 2881
Size: 2418 3982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6400
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PmlI CAC'GTG
Cuts at: 3556 3556
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 2781 3970 2781
Size: 1189 5211
PstI C_TGCA'G
Cuts at: 830 3752 830
Size: 2922 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
SacI G_AGCT'C
Cuts at: 2132 2132
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 2429 4648 6360 2429
Size: 2219 1712 2469
Fragments arranged by size:
2469 2219 1712
SfcI C'TryA_G
Cuts at: 826 1045 3748 4883 5564 5755 826
Size: 219 2703 1135 681 191 1471
Fragments arranged by size:
2703 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 2135 2642 3727 1872
Size: 263 507 1085 4545
Fragments arranged by size:
4545 1085 507 263
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 4504 5405 5667 820
Size: 197 926 570 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 4324 5
Size: 47 4272 2081
Fragments arranged by size:
4272 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6400
StyI C'CwwG_G
Cuts at: 513 1065 3839 513
Size: 552 2774 3074
Fragments arranged by size:
3074 2774 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4427 4444 4597 4782 5227 325
Size: 1255 2847 17 153 185 445 1498
Fragments arranged by size:
2847 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 2914 3466
Size: 274 80 33 51 1443 450 487 552
Cuts at: 3466 3475 4646 6358 96
Size: 9 1171 1712 138
Fragments arranged by size:
1712 1443 1171 552 487 450 274 138
80 51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6360 6392 6360
Size: 32 6368
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6400
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 2133 2640 3725 1870
Size: 263 507 1085 4545
Fragments arranged by size:
4545 1085 507 263
XmnI GAAnn'nnTTC
Cuts at: 3323 3386 4529 3323
Size: 63 1143 5194
Fragments arranged by size:
5194 1143 63
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI
NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI