Restriction map for
pFN21AE5636
(Circular) (Six-base) MAPSORT of: pFN21AE5636.seq Check: 405 from: 1 to: 6151
pFN21AE5636 6151 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 2453 3472 2453
Size: 1019 5132
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 4214 5598 5866 1632
Size: 458 2124 1384 268 1917
Fragments arranged by size:
2124 1917 1384 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2121 1184
Size: 937 5214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3358 3390 3358
Size: 32 6119
AlwNI CAG_nnn'CTG
Cuts at: 3894 5466 3894
Size: 1572 4579
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2258 2903 3017 3460 3686 3838 5947 1493
Size: 765 645 114 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 765 645 443 226 152 114
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2033 2738 3476 1870
Size: 73 72 18 705 738 4545
Fragments arranged by size:
4545 738 705 73 72 18
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 3472 4927 618
Size: 325 217 627 666 1019 1455 1842
Fragments arranged by size:
1842 1455 1019 666 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3470 1391
Size: 2079 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3882 4840 442
Size: 416 53 83 399 24 2465 958 1753
Fragments arranged by size:
2465 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 3036 4811 1239
Size: 1460 337 1775 2579
Fragments arranged by size:
2579 1775 1460 337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4814 882
Size: 617 3315 2219
Fragments arranged by size:
3315 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2570 3942 493
Size: 711 1366 1372 2702
Fragments arranged by size:
2702 1372 1366 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3958 4340 4975 275
Size: 53 83 186 936 2425 382 635 1451
Fragments arranged by size:
2425 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3358 3388 3397 3427 1178
Size: 30 165 30 1955 30 9 30 3902
Fragments arranged by size:
3902 1955 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2237 2381 2384 3058 3418 1186
Size: 976 75 144 3 674 360 3919
Fragments arranged by size:
3919 976 674 360 144 75 3
BseYI C'CCAG_C
Cuts at: 5354 5850 5354
Size: 496 5655
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2665 1163
Size: 573 929 4649
Fragments arranged by size:
4649 929 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 3662 3755 2846
Size: 816 93 5242
Fragments arranged by size:
5242 816 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3470 4212 4297 5368 1391
Size: 399 1680 742 85 1071 2174
Fragments arranged by size:
2174 1680 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3506 844
Size: 2662 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2815 4646 4820 65
Size: 1183 1567 1831 174 1396
Fragments arranged by size:
1831 1567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 794
Size: 1625 4526
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146
Size: 1509 367 385 17 1452 86 12 346
Cuts at: 6146 1972
Size: 1977
Fragments arranged by size:
1977 1509 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2801 2801
Size: 6151
BtgI C'CryG_G
Cuts at: 513 1065 2004 2481 513
Size: 552 939 477 4183
Fragments arranged by size:
4183 939 552 477
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2648 2903 2977 3667 4461 4481 2648
Size: 255 74 690 794 20 4318
Fragments arranged by size:
4318 794 690 255 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116
Size: 54 1425 50 275 18 1768 889 1629
Cuts at: 6116 8
Size: 43
Fragments arranged by size:
1768 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2178 2277 2358 4081 1353
Size: 786 39 99 81 1723 3423
Fragments arranged by size:
3423 1723 786 99 81 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2699 3036 4214 4811 5598 5866
Size: 393 458 609 337 1178 597 787 268
Cuts at: 5866 1239
Size: 1524
Fragments arranged by size:
1524 1178 787 609 597 458 393 337
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2072
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 3384 3384
Size: 6151
EcoRI G'AATT_C
Cuts at: 2258 2903 3017 3460 3838 2258
Size: 645 114 443 378 4571
Fragments arranged by size:
4571 645 443 378 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2870 2870
Size: 6151
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 4792 4824 4866
Size: 32 135 32 838 32 2350 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 2350 838 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2406 748
Size: 1658 4493
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KpnI G_GTAC'C
Cuts at: 2457 3476 2457
Size: 1019 5132
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 2811 5265 5449 1305
Size: 1119 387 2454 184 2007
Fragments arranged by size:
2454 2007 1119 387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4109 4468 4627 518
Size: 648 2943 359 159 2042
Fragments arranged by size:
2943 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2781 2893 3567 4244 5392 5637 1606
Size: 1175 112 674 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 674 245 112
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2620 3509 5054 1132
Size: 97 1391 889 1545 2229
Fragments arranged by size:
2229 1545 1391 889 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3358 3390 4220 4252 5733 5765 3358
Size: 32 830 32 1481 32 3744
Fragments arranged by size:
3744 1481 830 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3384 3384
Size: 6151
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PstI C_TGCA'G
Cuts at: 830 2898 3503 830
Size: 2068 605 3478
Fragments arranged by size:
3478 2068 605
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2781 2893 2781
Size: 112 6039
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2527 2894 3124 3499 4634 5315 5506
Size: 219 1482 367 230 375 1135 681 191
Cuts at: 5506 826
Size: 1471
Fragments arranged by size:
1482 1471 1135 681 375 367 230 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4255 5156 5418 820
Size: 197 926 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3590 513
Size: 552 939 1586 3074
Fragments arranged by size:
3074 1586 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4178 4195 4348 4533 4978 325
Size: 1255 2598 17 153 185 445 1498
Fragments arranged by size:
2598 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 2682 4397
Size: 274 80 33 51 1443 78 627 1715
Cuts at: 4397 6109 96
Size: 1712 138
Fragments arranged by size:
1715 1712 1443 627 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI