Restriction map for
pFN21AE5631
(Circular) (Six-base) MAPSORT of: pFN21AE5631.seq Check: 8137 from: 1 to: 6136
pFN21AE5631 6136 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 2221 4865 2221
Size: 2644 3492
AleI CACnn'nnGTG
Cuts at: 1166 2603 2834 1166
Size: 1437 231 4468
Fragments arranged by size:
4468 1437 231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AlwNI CAG_nnn'CTG
Cuts at: 2212 2634 3103 3233 3262 3879 5451 2212
Size: 422 469 130 29 617 1572 2897
Fragments arranged by size:
2897 1572 617 469 422 130 29
ApaI G_GGCC'C
Cuts at: 2644 2722 2644
Size: 78 6058
ApaLI G'TGCA_C
Cuts at: 3023 4193 5349 3023
Size: 1170 1156 3810
Fragments arranged by size:
3810 1170 1156
ApoI r'AATT_y
Cuts at: 1493 3445 3671 3823 5932 1493
Size: 1952 226 152 2109 1697
Fragments arranged by size:
2109 1952 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2185 2384 2777 3008 3143 3461 1870
Size: 73 242 199 393 231 135 318 4545
Fragments arranged by size:
4545 393 318 242 231 199 135 73
BamHI G'GATC_C
Cuts at: 3247 3466 3833 3247
Size: 219 367 5550
Fragments arranged by size:
5550 367 219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3093 3383 3457 4912
Size: 325 217 627 246 1060 290 74 1455
Cuts at: 4912 618
Size: 1842
Fragments arranged by size:
1842 1455 1060 627 325 290 246 217
74
BanII G_rGCy'C
Cuts at: 1391 2012 2052 2451 2644 2722 3455 1391
Size: 621 40 399 193 78 733 4072
Fragments arranged by size:
4072 733 621 399 193 78 40
BbeI G_GCGC'C
Cuts at: 2037 3387 2037
Size: 1350 4786
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 3123 928
Size: 971 590 634 3941
Fragments arranged by size:
3941 971 634 590
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2822 3324
Size: 107 122 71 631 605 566 584 502
Cuts at: 3324 4747 136
Size: 1423 1525
Fragments arranged by size:
1525 1423 631 605 584 566 502 122
107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3263 3553 3263
Size: 290 5846
Bme1580I G_kGCm'C
Cuts at: 1790 2644 2722 3027 4197 5353 1790
Size: 854 78 305 1170 1156 2573
Fragments arranged by size:
2573 1170 1156 854 305 78
BmgBI CAC'GTC
Cuts at: 1318 3289 1318
Size: 1971 4165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2480 2635 2840 4796 1239
Size: 760 481 155 205 1956 2579
Fragments arranged by size:
2579 1956 760 481 205 155
Bpu10I CC'TnA_GC
Cuts at: 2426 3410 2426
Size: 984 5152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2197 3175 3413 4799 882
Size: 617 698 978 238 1386 2219
Fragments arranged by size:
2219 1386 978 698 617 238
BsaAI yAC'GTr
Cuts at: 493 1204 2362 2551 3927 493
Size: 711 1158 189 1376 2702
Fragments arranged by size:
2702 1376 1158 711 189
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 3384 3943 4325
Size: 53 83 186 936 501 1350 559 382
Cuts at: 4325 4960 275
Size: 635 1451
Fragments arranged by size:
1451 1350 936 635 559 501 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2337 2585 4569 4967 5241 5388 5799 2337
Size: 248 1984 398 274 147 411 2674
Fragments arranged by size:
2674 1984 411 398 274 248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2093 2123 3314 3344 1178
Size: 30 165 30 690 30 1191 30 3970
Fragments arranged by size:
3970 1191 690 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2002 1186
Size: 816 5320
BseYI C'CCAG_C
Cuts at: 2627 2697 2767 3059 5339 5835 2627
Size: 70 70 292 2280 496 2928
Fragments arranged by size:
2928 2280 496 292 70 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3395 1163
Size: 573 1659 3904
Fragments arranged by size:
3904 1659 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2898 3586 4347 5375 1540
Size: 275 186 897 688 761 1028 2301
Fragments arranged by size:
2301 1028 897 761 688 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2349 3027 3314 4197 4282 5353 2012
Size: 337 678 287 883 85 1071 2795
Fragments arranged by size:
2795 1071 883 678 337 287 85
BsmI GAATG_Cn'
Cuts at: 3647 3740 3647
Size: 93 6043
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2052 2349 2451 2644 2722 3027
Size: 399 222 40 297 102 193 78 305
Cuts at: 3027 3314 3455 4197 4282 5353 1391
Size: 287 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 297 287 222
193 141 102 85 78 40
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 3491 844
Size: 1268 1379 3489
Fragments arranged by size:
3489 1379 1268
BsrBI CCG'CTC
Cuts at: 1394 1823 2673 4023 1394
Size: 429 850 1350 3507
Fragments arranged by size:
3507 1350 850 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3100 794
Size: 2306 3830
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 2845 3074 1184
Size: 1661 229 4246
Fragments arranged by size:
4246 1661 229
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 3289 2836
Size: 453 5683
BstYI r'GATC_y
Cuts at: 1972 3247 3466 3833 4218 4235 5687 5773 5785
Size: 1275 219 367 385 17 1452 86 12
Cuts at: 5785 6131 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1275 385 367 346 219 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2256 2415 2604 2958 3336 513
Size: 552 1191 159 189 354 378 3313
Fragments arranged by size:
3313 1191 552 378 354 189 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 487
Size: 683 56 1286 4111
Fragments arranged by size:
4111 1286 683 56
BtsI GCAGTG_nn'
Cuts at: 2514 3249 3652 4446 4466 2514
Size: 735 403 794 20 4184
Fragments arranged by size:
4184 794 735 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2420 2455 529
Size: 35 1856 35 4210
Fragments arranged by size:
4210 1856 35 35
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DraIII CAC_nnn'GTG
Cuts at: 3022 3022
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2462 2552 5143 809
Size: 507 1146 90 2591 1802
Fragments arranged by size:
2591 1802 1146 507 90
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2895 3348 3396
Size: 54 1425 50 275 18 1065 453 48
Cuts at: 3396 3583 4472 6101 8
Size: 187 889 1629 43
Fragments arranged by size:
1629 1425 1065 889 453 275 187 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2895 3583 1537
Size: 275 1083 688 4090
Fragments arranged by size:
4090 1083 688 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2485 2666 4066 1353
Size: 1132 181 1400 3423
Fragments arranged by size:
3423 1400 1132 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2939 4723 5097 5243 387
Size: 1064 138 1350 1784 374 146 1280
Fragments arranged by size:
1784 1350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2480 2635 2840 4199 4796 5583
Size: 393 367 481 155 205 1359 597 787
Cuts at: 5583 5851 1239
Size: 268 1524
Fragments arranged by size:
1524 1359 787 597 481 393 367 268
205 155
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2663 3225 2204
Size: 459 562 5115
Fragments arranged by size:
5115 562 459
EcoO109I rG'GnC_Cy
Cuts at: 2182 2209 2616 2640 2785 3139 3325 2182
Size: 27 407 24 145 354 186 4993
Fragments arranged by size:
4993 407 354 186 145 27 24
EcoRI G'AATT_C
Cuts at: 3445 3823 3445
Size: 378 5758
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 2037 3270 3387 5283 1678
Size: 359 1233 117 1896 2531
Fragments arranged by size:
2531 1896 1233 359 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 2608 2640 4777
Size: 32 135 32 635 32 369 32 2137
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2137 635 369 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KasI G'GCGC_C
Cuts at: 2033 3383 2033
Size: 1350 4786
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5250 5434 1305
Size: 3945 184 2007
Fragments arranged by size:
3945 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3398 10
Size: 54 1425 343 1566 2748
Fragments arranged by size:
2748 1566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2603 2834 2933 3287 4094 4453
Size: 648 900 537 231 99 354 807 359
Cuts at: 4453 4612 518
Size: 159 2042
Fragments arranged by size:
2042 900 807 648 537 359 354 231
159 99
MspA1I CmG'CkG
Cuts at: 1606 2634 2894 2989 3100 3262 3346 3552 4229
Size: 1028 260 95 111 162 84 206 677
Cuts at: 4229 5377 5622 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1028 677 260 245 206 162
111 95 84
NarI GG'CG_CC
Cuts at: 2034 3384 2034
Size: 1350 4786
NcoI C'CATG_G
Cuts at: 513 1065 2415 3336 513
Size: 552 1350 921 3313
Fragments arranged by size:
3313 1350 921 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2443 1108
Size: 1104 231 4801
Fragments arranged by size:
4801 1104 231
PfoI T'CCnGG_A
Cuts at: 2199 2451 3135 2199
Size: 252 684 5200
Fragments arranged by size:
5200 684 252
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PmlI CAC'GTG
Cuts at: 2362 2362
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2616 3139 2209
Size: 407 523 5206
Fragments arranged by size:
5206 523 407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2640 2718 2640
Size: 78 6058
PspXI vC'TCGA_Gb
Cuts at: 3143 3143
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2166 3488 830
Size: 1336 1322 3478
Fragments arranged by size:
3478 1336 1322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2634 2989 3100 2634
Size: 355 111 5670
Fragments arranged by size:
5670 355 111
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SanDI GG'GwC_CC
Cuts at: 2616 2616
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SfcI C'TryA_G
Cuts at: 826 1045 2162 2539 2592 3484 4619 5300 5491
Size: 219 1117 377 53 892 1135 681 191
Cuts at: 5491 826
Size: 1471
Fragments arranged by size:
1471 1135 1117 892 681 377 219 191
53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2238 2238
Size: 6136
SfoI GGC'GCC
Cuts at: 2035 3385 2035
Size: 1350 4786
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 2187 2386 2779 3463 1872
Size: 315 199 393 684 4545
Fragments arranged by size:
4545 684 393 315 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3143 4240 5141 5403 820
Size: 197 926 1200 1097 901 262 1553
Fragments arranged by size:
1553 1200 1097 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 1517
Size: 715 5421
StyI C'CwwG_G
Cuts at: 513 1065 2415 2431 3336 3387 3575 513
Size: 552 1350 16 905 51 188 3074
Fragments arranged by size:
3074 1350 905 552 188 51 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2163 2308 2828 4163 4180 4333 4518
Size: 1255 583 145 520 1335 17 153 185
Cuts at: 4518 4963 325
Size: 445 1498
Fragments arranged by size:
1498 1335 1255 583 520 445 185 153
145 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4382 6094 96
Size: 274 80 33 51 1443 2405 1712 138
Fragments arranged by size:
2405 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 2864 2903 1316
Size: 1548 39 4549
Fragments arranged by size:
4549 1548 39
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 3343 2359
Size: 984 5152
XhoI C'TCGA_G
Cuts at: 1943 3143 1943
Size: 1200 4936
XmaI C'CCGG_G
Cuts at: 1870 2185 2384 2777 3461 1870
Size: 315 199 393 684 4545
Fragments arranged by size:
4545 684 393 315 199
XmnI GAAnn'nnTTC
Cuts at: 4265 4265
Size: 6136
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII
SacII SapI SexAI SrfI SwaI