Restriction map for
pFN21AE5625
(Circular) (Six-base) MAPSORT of: pFN21AE5625.seq Check: 8451 from: 1 to: 6184
pFN21AE5625 6184 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3239 3527 1937
Size: 1302 288 4594
Fragments arranged by size:
4594 1302 288
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 3003 3063 4247 5631 5899 1632
Size: 627 744 60 1184 1384 268 1917
Fragments arranged by size:
1917 1384 1184 744 627 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6184
AlwNI CAG_nnn'CTG
Cuts at: 2405 2983 3927 5499 2405
Size: 578 944 1572 3090
Fragments arranged by size:
3090 1572 944 578
ApaI G_GGCC'C
Cuts at: 3392 3392
Size: 6184
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 2931 3493 3719 3871 5980 1493
Size: 1438 562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1438 562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 2925 3509 1870
Size: 73 787 195 584 4545
Fragments arranged by size:
4545 787 584 195 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3215 3248 3215
Size: 33 6151
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2954 3135 3505 4960 618
Size: 325 217 627 1167 181 370 1455 1842
Fragments arranged by size:
1842 1455 1167 627 370 325 217 181
BanII G_rGCy'C
Cuts at: 1391 2293 3341 3392 3400 3503 1391
Size: 902 1048 51 8 103 4072
Fragments arranged by size:
4072 1048 902 103 51 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 2251 2913 928
Size: 971 316 36 662 4199
Fragments arranged by size:
4199 971 662 316 36
BbvCI CC'TCA_GC
Cuts at: 2426 2577 2426
Size: 151 6033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2996 4795 136
Size: 107 122 71 631 605 1324 1799 1525
Fragments arranged by size:
1799 1525 1324 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6179 6179
Size: 6184
BlpI GC'TnA_GC
Cuts at: 2183 3601 2183
Size: 1418 4766
Bme1580I G_kGCm'C
Cuts at: 1790 3392 4245 5401 1790
Size: 1602 853 1156 2573
Fragments arranged by size:
2573 1602 1156 853
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2911 3915 4873
Size: 416 53 83 399 24 1494 1004 958
Cuts at: 4873 442
Size: 1753
Fragments arranged by size:
1753 1494 1004 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3410 1056
Size: 2354 3830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3384 3384
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2903 4844 1239
Size: 1565 99 1941 2579
Fragments arranged by size:
2579 1941 1565 99
Bpu10I CC'TnA_GC
Cuts at: 2426 2501 2577 3399 2426
Size: 75 76 822 5211
Fragments arranged by size:
5211 822 76 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2747 4309 5174 5472 2129
Size: 618 1562 865 298 2841
Fragments arranged by size:
2841 1562 865 618 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2963 4847 882
Size: 617 1464 1884 2219
Fragments arranged by size:
2219 1884 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 2474 6178 2474
Size: 3704 2480
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3084 4617 5015 5289 5436 5847 3084
Size: 1533 398 274 147 411 3421
Fragments arranged by size:
3421 1533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2582 2801 2981 3193 3347 1186
Size: 958 438 219 180 212 154 4023
Fragments arranged by size:
4023 958 438 219 212 180 154
BseYI C'CCAG_C
Cuts at: 2991 3097 3325 3480 5387 5883 2991
Size: 106 228 155 1907 496 3292
Fragments arranged by size:
3292 1907 496 228 155 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2840 1163
Size: 573 1104 4507
Fragments arranged by size:
4507 1104 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2184 3400 4245 4330 5401 2184
Size: 1216 845 85 1071 2967
Fragments arranged by size:
2967 1216 1071 845 85
BsmI GAATG_Cn'
Cuts at: 3695 3788 3695
Size: 93 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2293 3341 3392 3400 3503 4245
Size: 399 394 109 1048 51 8 103 742
Cuts at: 4245 4330 5401 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1048 742 399 394 109 103
85 51 8
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 2656 4073 5803 2656
Size: 1417 1730 3037
Fragments arranged by size:
3037 1730 1417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2471 3539 844
Size: 1627 1068 3489
Fragments arranged by size:
3489 1627 1068
BsrBI CCG'CTC
Cuts at: 1394 1823 2111 4071 1394
Size: 429 288 1960 3507
Fragments arranged by size:
3507 1960 429 288
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 2610 4679 4853 65
Size: 1183 1284 78 2069 174 1396
Fragments arranged by size:
2069 1396 1284 1183 174 78
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6184
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 3265 3383 3265
Size: 118 6066
BstYI r'GATC_y
Cuts at: 1972 2421 2651 3421 3514 3881 4266 4283 5735
Size: 449 230 770 93 367 385 17 1452
Cuts at: 5735 5821 5833 6179 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 770 449 385 367 346 230
93 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3315 3315
Size: 6184
BtgI C'CryG_G
Cuts at: 513 1065 2703 513
Size: 552 1638 3994
Fragments arranged by size:
3994 1638 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2469 2527 487
Size: 683 56 1243 58 4144
Fragments arranged by size:
4144 1243 683 58 56
BtsI GCAGTG_nn'
Cuts at: 3700 4494 4514 3700
Size: 794 20 5370
Fragments arranged by size:
5370 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 3631 4520
Size: 54 1425 50 275 18 471 1330 889
Cuts at: 4520 6149 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1330 889 471 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2719 4114 1353
Size: 729 637 1395 3423
Fragments arranged by size:
3423 1395 729 637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3284 4771 5145 5291 387
Size: 1064 138 1695 1487 374 146 1280
Fragments arranged by size:
1695 1487 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3398 3398
Size: 6184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2804 2903 3003 3063 4247 4844
Size: 393 627 545 99 100 60 1184 597
Cuts at: 4844 5631 5899 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1184 787 627 597 545 393 268
100 99 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2786 3314 2765
Size: 21 528 5635
Fragments arranged by size:
5635 528 21
EcoO109I rG'GnC_Cy
Cuts at: 3307 3388 3389 3307
Size: 81 1 6102
Fragments arranged by size:
6102 81 1
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 3454 2872
Size: 582 5602
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 5331 1678
Size: 3653 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 4825 4857 4899
Size: 32 135 32 1037 32 2184 32 42
Cuts at: 4899 4931 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 1037 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2341 3528 3774 669
Size: 988 281 403 1187 246 3079
Fragments arranged by size:
3079 1187 988 403 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2692 5298 5482 1305
Size: 1387 2606 184 2007
Fragments arranged by size:
2606 2007 1387 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 10
Size: 54 1425 343 471 3891
Fragments arranged by size:
3891 1425 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3065 4142 4501 4660 518
Size: 648 1899 1077 359 159 2042
Fragments arranged by size:
2042 1899 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2592 2881 3041 3600 4277 5425 5670 1606
Size: 986 289 160 559 677 1148 245 2120
Fragments arranged by size:
2120 1148 986 677 559 289 245 160
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 3406 1052
Size: 2354 3830
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3542 5087 1132
Size: 97 2313 1545 2229
Fragments arranged by size:
2313 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2893 3383 1348
Size: 1545 490 4149
Fragments arranged by size:
4149 1545 490
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspOMI G'GGCC_C
Cuts at: 3388 3388
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3283 3536 830
Size: 2453 253 3478
Fragments arranged by size:
3478 2453 253
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2592 2881 3041 2592
Size: 289 160 5735
Fragments arranged by size:
5735 289 160
SacI G_AGCT'C
Cuts at: 3400 3400
Size: 6184
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 2852 4432 6144 2852
Size: 1580 1712 2892
Fragments arranged by size:
2892 1712 1580
SfcI C'TryA_G
Cuts at: 826 1045 3279 3532 4667 5348 5539 826
Size: 219 2234 253 1135 681 191 1471
Fragments arranged by size:
2234 1471 1135 681 253 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2927 3511 1872
Size: 1055 584 4545
Fragments arranged by size:
4545 1055 584
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2762 4288 5189 5451 820
Size: 197 926 201 618 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 618 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 2363 4108 5
Size: 47 2311 1745 2081
Fragments arranged by size:
2311 2081 1745 47
StuI AGG'CCT
Cuts at: 1517 2942 1517
Size: 1425 4759
StyI C'CwwG_G
Cuts at: 513 1065 2370 2950 3447 3623 513
Size: 552 1305 580 497 176 3074
Fragments arranged by size:
3074 1305 580 552 497 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3317 4211 4228 4381 4566 5011 325
Size: 1255 1737 894 17 153 185 445 1498
Fragments arranged by size:
1737 1498 1255 894 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2850 4430 6142
Size: 274 80 33 51 1443 873 1580 1712
Cuts at: 6142 96
Size: 138
Fragments arranged by size:
1712 1580 1443 873 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6144 6176 6144
Size: 32 6152
Tth111I GACn'n_nGTC
Cuts at: 1316 2198 1316
Size: 882 5302
XbaI T'CTAG_A
Cuts at: 3187 3520 3187
Size: 333 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2377 2980 2377
Size: 603 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2925 3509 1870
Size: 1055 584 4545
Fragments arranged by size:
4545 1055 584
XmnI GAAnn'nnTTC
Cuts at: 3174 4313 3174
Size: 1139 5045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI