Restriction map for
pFN21AE5623
(Circular) (Six-base) MAPSORT of: pFN21AE5623.seq Check: 5363 from: 1 to: 6127
pFN21AE5623 6127 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2796 4190 5574 5842 1632
Size: 1164 1394 1384 268 1917
Fragments arranged by size:
1917 1394 1384 1164 268
AfeI AGC'GCT
Cuts at: 2135 2135
Size: 6127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 3870 5442 3870
Size: 1572 4555
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2420 2690 3436 3662 3814 5923 1493
Size: 927 270 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 927 746 270 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 3452 1870
Size: 73 104 1405 4545
Fragments arranged by size:
4545 1405 104 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2375 2408 2375
Size: 33 6094
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3446 1391
Size: 2055 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BbvCI CC'TCA_GC
Cuts at: 2126 2126
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2194 2228 4318 4352 1903
Size: 34 257 34 2090 34 3678
Fragments arranged by size:
3678 2090 257 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 2097 1548
Size: 549 5578
BfrBI ATG'CAT
Cuts at: 2263 2263
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 4738 136
Size: 107 122 71 631 605 574 2492 1525
Fragments arranged by size:
2492 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 2407 2986 6122 2407
Size: 579 3136 2412
Fragments arranged by size:
3136 2412 579
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 4188 5344 1790
Size: 2398 1156 2573
Fragments arranged by size:
2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2496 4787 1239
Size: 1257 2291 2579
Fragments arranged by size:
2579 2291 1257
Bpu10I CC'TnA_GC
Cuts at: 2126 2126
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 3918 493
Size: 711 1296 1418 2702
Fragments arranged by size:
2702 1418 1296 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5902
Fragments arranged by size:
5902 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 1186
Size: 1084 5043
BseYI C'CCAG_C
Cuts at: 5330 5826 5330
Size: 496 5631
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3006 1163
Size: 573 1270 4284
Fragments arranged by size:
4284 1270 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4188 4273 5344 4188
Size: 85 1071 4971
Fragments arranged by size:
4971 1071 85
BsiWI C'GTAC_G
Cuts at: 2500 2500
Size: 6127
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3446 4188 4273 5344 1391
Size: 399 1656 742 85 1071 2174
Fragments arranged by size:
2174 1656 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 4014 1394
Size: 429 208 1983 3507
Fragments arranged by size:
3507 1983 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 2205 2310 3327 4622 4796 65
Size: 1183 957 105 1017 1295 174 1396
Fragments arranged by size:
1396 1295 1183 1017 957 174 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3029 794
Size: 2235 3892
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 3245 1184
Size: 2061 4066
BstYI r'GATC_y
Cuts at: 1972 2407 2839 2986 3457 3824 4209 4226 5678
Size: 435 432 147 471 367 385 17 1452
Cuts at: 5678 5764 5776 6122 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 471 435 432 385 367 346
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 4390
Fragments arranged by size:
4390 939 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 3574 4463
Size: 54 1425 50 275 18 436 1308 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1308 889 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 4714 5088 5234 387
Size: 1064 138 444 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2496 2796 4190 4787 5574 5842 1239
Size: 393 864 300 1394 597 787 268 1524
Fragments arranged by size:
1524 1394 864 787 597 393 300 268
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
EcoRV GAT'ATC
Cuts at: 2440 2440
Size: 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2784 2801 3194 3281 2784
Size: 17 393 87 5630
Fragments arranged by size:
5630 393 87 17
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2137 5274 1678
Size: 459 3137 2531
Fragments arranged by size:
3137 2531 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4768 4800 4842 4874 1373
Size: 32 135 32 3196 32 42 32 2626
Fragments arranged by size:
3196 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2637 748
Size: 1889 4238
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2268 10
Size: 54 1425 343 436 3869
Fragments arranged by size:
3869 1425 436 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2343 4085 4444 4603 518
Size: 648 1002 175 1742 359 159 2042
Fragments arranged by size:
2042 1742 1002 648 359 175 159
MspA1I CmG'CkG
Cuts at: 1606 3543 4220 5368 5613 1606
Size: 1937 677 1148 245 2120
Fragments arranged by size:
2120 1937 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2250 513
Size: 552 939 246 4390
Fragments arranged by size:
4390 939 552 246
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2265 2265
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 3485 5030 1132
Size: 97 2256 1545 2229
Fragments arranged by size:
2256 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PfoI T'CCnGG_A
Cuts at: 2157 2157
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2748 2780 2748
Size: 32 6095
PstI C_TGCA'G
Cuts at: 830 2246 3479 830
Size: 1416 1233 3478
Fragments arranged by size:
3478 1416 1233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 2246 3479 2246
Size: 1233 4894
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2242 3475 4610 5291 5482 826
Size: 219 1197 1233 1135 681 191 1471
Fragments arranged by size:
1471 1233 1197 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2246 2246
Size: 6127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 2049 3454 1872
Size: 177 1405 4545
Fragments arranged by size:
4545 1405 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4231 5132 5394 820
Size: 197 926 2288 901 262 1553
Fragments arranged by size:
2288 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2500 3918 493
Size: 2007 1418 2702
Fragments arranged by size:
2702 2007 1418
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SrfI GCCC'GGGC
Cuts at: 2049 2049
Size: 6127
SspI AAT'ATT
Cuts at: 5 52 2822 4051 5
Size: 47 2770 1229 2081
Fragments arranged by size:
2770 2081 1229 47
StuI AGG'CCT
Cuts at: 1517 2240 1517
Size: 723 5404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2199 2250 3566 513
Size: 552 939 186 9 51 1316 3074
Fragments arranged by size:
3074 1316 939 552 186 51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 4154 4171 4324 4509 4954 325
Size: 1255 578 1996 17 153 185 445 1498
Fragments arranged by size:
1996 1498 1255 578 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 3124 4373
Size: 274 80 33 51 1443 129 1018 1249
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1249 1018 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3267 3299 6087 6119 3267
Size: 32 2788 32 3275
Fragments arranged by size:
3275 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 2047 3452 1870
Size: 177 1405 4545
Fragments arranged by size:
4545 1405 177
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsmBI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfoI SwaI XcmI