Restriction map for
pFN21AE5622
(Circular) (Six-base) MAPSORT of: pFN21AE5622.seq Check: 3115 from: 1 to: 6313
pFN21AE5622 6313 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2620 2620
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 3656 1937
Size: 388 1331 4594
Fragments arranged by size:
4594 1331 388
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4376 5760 6028 1632
Size: 2744 1384 268 1917
Fragments arranged by size:
2744 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 2965 5212 6189 1203
Size: 1762 2247 977 1327
Fragments arranged by size:
2247 1762 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3556 1184
Size: 2372 3941
AlwNI CAG_nnn'CTG
Cuts at: 2255 4056 5628 2255
Size: 1801 1572 2940
Fragments arranged by size:
2940 1801 1572
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 3622 3848 4000 6109 1493
Size: 2129 226 152 2109 1697
Fragments arranged by size:
2129 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
BamHI G'GATC_C
Cuts at: 2834 3643 4010 2834
Size: 809 367 5137
Fragments arranged by size:
5137 809 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2602 3634 5089 618
Size: 325 217 627 815 1032 1455 1842
Fragments arranged by size:
1842 1455 1032 815 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3632 1391
Size: 2241 4072
BbeI G_GCGC'C
Cuts at: 2606 2606
Size: 6313
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 2107 2692 3306 1548
Size: 559 585 614 4555
Fragments arranged by size:
4555 614 585 559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4924 136
Size: 107 122 71 631 605 3252 1525
Fragments arranged by size:
3252 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 4374 5530 1790
Size: 2584 1156 2573
Fragments arranged by size:
2584 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4044 5002 442
Size: 416 53 83 399 24 2627 958 1753
Fragments arranged by size:
2627 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2494 2655 4973 1239
Size: 1255 161 2318 2579
Fragments arranged by size:
2579 2318 1255 161
Bpu10I CC'TnA_GC
Cuts at: 2881 3043 3593 2881
Size: 162 550 5601
Fragments arranged by size:
5601 550 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3025 4438 5303 5601 3025
Size: 1413 865 298 3737
Fragments arranged by size:
3737 1413 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2631 3176 4976 882
Size: 617 1132 545 1800 2219
Fragments arranged by size:
2219 1800 1132 617 545
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 2925 6307 2925
Size: 3382 2931
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2603 4120 4502 5137
Size: 53 83 186 936 1070 1517 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
1517 1451 1070 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3123 4746 5144 5418 5565 5976 3123
Size: 1623 398 274 147 411 3460
Fragments arranged by size:
3460 1623 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 4969
Fragments arranged by size:
4969 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2963 3164 1186
Size: 1777 201 4335
Fragments arranged by size:
4335 1777 201
BseYI C'CCAG_C
Cuts at: 2504 3318 5516 6012 2504
Size: 814 2198 496 2805
Fragments arranged by size:
2805 2198 814 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2592 1163
Size: 573 856 4884
Fragments arranged by size:
4884 856 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2555 4374 4459 5530 2555
Size: 1819 85 1071 3338
Fragments arranged by size:
3338 1819 1071 85
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2555 3632 4374 4459 5530 1391
Size: 399 765 1077 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 765 742 399 85
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2620 3668 844
Size: 1776 1048 3489
Fragments arranged by size:
3489 1776 1048
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4808 4982 65
Size: 1183 3560 174 1396
Fragments arranged by size:
3560 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2348 2852 3322 3584 2348
Size: 504 470 262 5077
Fragments arranged by size:
5077 504 470 262
BstYI r'GATC_y
Cuts at: 1972 2834 3643 4010 4395 4412 5864 5950 5962
Size: 862 809 367 385 17 1452 86 12
Cuts at: 5962 6308 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 862 809 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2331 2331
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2004 3061 513
Size: 552 939 1057 3765
Fragments arranged by size:
3765 1057 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3197 3829 4623 4643 3197
Size: 632 794 20 4867
Fragments arranged by size:
4867 794 632 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3148 3183 529
Size: 35 2584 35 3659
Fragments arranged by size:
3659 2584 35 35
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2995 3336 3760
Size: 54 1425 50 275 18 1165 341 424
Cuts at: 3760 4649 6278 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1165 889 424 341 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 3575 4243 1353
Size: 654 1568 668 3423
Fragments arranged by size:
3423 1568 668 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2494 2655 4376 4973 5760 6028 1239
Size: 393 862 161 1721 597 787 268 1524
Fragments arranged by size:
1721 1524 862 787 597 393 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 3338 3338
Size: 6313
EcoO109I rG'GnC_Cy
Cuts at: 2860 2860
Size: 6313
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
EcoRV GAT'ATC
Cuts at: 2391 2391
Size: 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2151 2673 2151
Size: 522 5791
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2606 5460 1678
Size: 928 2854 2531
Fragments arranged by size:
2854 2531 928
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2681 2713 4954
Size: 32 135 32 620 32 457 32 2241
Cuts at: 4954 4986 5028 5060 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2241 620 457 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KasI G'GCGC_C
Cuts at: 2602 2602
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 2406 3912 2406
Size: 1506 4807
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 5427 5611 1305
Size: 1257 2865 184 2007
Fragments arranged by size:
2865 2007 1257 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2997 3338 10
Size: 54 1425 343 1165 341 2985
Fragments arranged by size:
2985 1425 1165 343 341 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4271 4630 4789 518
Size: 648 3105 359 159 2042
Fragments arranged by size:
3105 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3322 3729 4406 5554 5799 1606
Size: 1716 407 677 1148 245 2120
Fragments arranged by size:
2120 1716 1148 677 407 245
NarI GG'CG_CC
Cuts at: 2603 2603
Size: 6313
NcoI C'CATG_G
Cuts at: 513 1065 2004 3061 513
Size: 552 939 1057 3765
Fragments arranged by size:
3765 1057 939 552
NdeI CA'TA_TG
Cuts at: 387 3460 387
Size: 3073 3240
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 2172 2969 3671 5216 1132
Size: 97 943 797 702 1545 2229
Fragments arranged by size:
2229 1545 943 797 702 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 2965 5212 2965
Size: 2247 4066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6313
PfoI T'CCnGG_A
Cuts at: 2699 2699
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 6313
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PstI C_TGCA'G
Cuts at: 830 2617 3204 3665 830
Size: 1787 587 461 3478
Fragments arranged by size:
3478 1787 587 461
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 3322 3322
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SapI GCTCTTCn'nnn_
Cuts at: 2007 2007
Size: 6313
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 2958 2958
Size: 6313
SfcI C'TryA_G
Cuts at: 826 1045 2300 2613 3013 3200 3388 3661 4796
Size: 219 1255 313 400 187 188 273 1135
Cuts at: 4796 5477 5668 826
Size: 681 191 1471
Fragments arranged by size:
1471 1255 1135 681 400 313 273 219
191 188 187
SfoI GGC'GCC
Cuts at: 2604 2604
Size: 6313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3004 4417 5318 5580 820
Size: 197 926 1061 1413 901 262 1553
Fragments arranged by size:
1553 1413 1061 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 3610 152
Size: 3458 2855
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 2416 3369 4237 5
Size: 47 2364 953 868 2081
Fragments arranged by size:
2364 2081 953 868 47
StuI AGG'CCT
Cuts at: 1517 2495 1517
Size: 978 5335
StyI C'CwwG_G
Cuts at: 513 1065 2004 3061 3339 3752 513
Size: 552 939 1057 278 413 3074
Fragments arranged by size:
3074 1057 939 552 413 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3065 3155 3332 4340 4357 4510 4695
Size: 1255 1485 90 177 1008 17 153 185
Cuts at: 4695 5140 325
Size: 445 1498
Fragments arranged by size:
1498 1485 1255 1008 445 185 177 153
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4559 6271 96
Size: 274 80 33 51 1443 2582 1712 138
Fragments arranged by size:
2582 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6273 6305 6273
Size: 32 6281
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 3369 4442 3369
Size: 1073 5240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI
XcmI